Caenorhabditis elegans

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28314 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O61919|O61919_CAEEL Glycosyltransferase family 92 protein OS=Caenorhabditis elegans OX=6239 GN=C18G1.6 PE=3 SV=1
MM1 pKa = 7.71LMLTFASTSSDD12 pKa = 4.19LLPMSNVFDD21 pKa = 3.85VQSSVFYY28 pKa = 10.67NEE30 pKa = 4.27KK31 pKa = 10.85NMFYY35 pKa = 10.63SSSQDD40 pKa = 3.17FSSCEE45 pKa = 3.99DD46 pKa = 3.1SSQFADD52 pKa = 3.86DD53 pKa = 4.88SGFFDD58 pKa = 4.84DD59 pKa = 6.24SEE61 pKa = 4.39ISSIGYY67 pKa = 9.65EE68 pKa = 3.89IGSKK72 pKa = 9.68LAAMCDD78 pKa = 3.71DD79 pKa = 5.37FDD81 pKa = 5.08AQMMSYY87 pKa = 9.93SAHH90 pKa = 6.27ASDD93 pKa = 4.2RR94 pKa = 11.84SLFHH98 pKa = 7.22RR99 pKa = 11.84LLDD102 pKa = 4.1FFAFF106 pKa = 4.14

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q17749|Q17749_CAEEL Uncharacterized protein OS=Caenorhabditis elegans OX=6239 GN=C06G3.8 PE=1 SV=1
MM1 pKa = 7.76PKK3 pKa = 10.24SKK5 pKa = 10.31SQKK8 pKa = 9.3NKK10 pKa = 9.77LRR12 pKa = 11.84PRR14 pKa = 11.84DD15 pKa = 3.62SKK17 pKa = 11.69GRR19 pKa = 11.84FTPLAKK25 pKa = 9.69TKK27 pKa = 9.08RR28 pKa = 11.84TVVSRR33 pKa = 11.84SRR35 pKa = 11.84SRR37 pKa = 11.84SASRR41 pKa = 11.84ARR43 pKa = 11.84RR44 pKa = 11.84SRR46 pKa = 11.84SVGRR50 pKa = 11.84PASRR54 pKa = 11.84RR55 pKa = 11.84ISVRR59 pKa = 11.84SVSTHH64 pKa = 4.64TAKK67 pKa = 10.76SPSLVVVISPSPRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84SSTSRR88 pKa = 11.84SSRR91 pKa = 11.84RR92 pKa = 11.84SVVRR96 pKa = 11.84SASRR100 pKa = 11.84GSVSRR105 pKa = 11.84RR106 pKa = 11.84SSSRR110 pKa = 11.84GSVYY114 pKa = 10.61GRR116 pKa = 11.84TGRR119 pKa = 11.84SRR121 pKa = 11.84SSKK124 pKa = 9.41RR125 pKa = 11.84SRR127 pKa = 11.84SRR129 pKa = 11.84SRR131 pKa = 11.84SRR133 pKa = 11.84SRR135 pKa = 11.84SRR137 pKa = 11.84SRR139 pKa = 11.84SYY141 pKa = 10.76SRR143 pKa = 11.84SRR145 pKa = 11.84SGSRR149 pKa = 11.84RR150 pKa = 11.84SSRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84SRR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84SSLKK163 pKa = 9.91ARR165 pKa = 11.84GRR167 pKa = 11.84RR168 pKa = 11.84ASTAKK173 pKa = 8.92KK174 pKa = 8.07TPAKK178 pKa = 9.72RR179 pKa = 11.84RR180 pKa = 11.84SRR182 pKa = 11.84SRR184 pKa = 11.84SQQKK188 pKa = 9.36VKK190 pKa = 10.86RR191 pKa = 11.84SMSRR195 pKa = 11.84KK196 pKa = 9.01GG197 pKa = 3.21

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19813

8501

28314

13466555

9

18562

475.6

53.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.519 ± 0.017

1.951 ± 0.015

5.444 ± 0.018

6.784 ± 0.031

4.373 ± 0.018

5.432 ± 0.022

2.33 ± 0.009

5.854 ± 0.015

6.216 ± 0.024

8.364 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.011

4.778 ± 0.012

5.164 ± 0.022

4.319 ± 0.018

5.282 ± 0.016

8.281 ± 0.023

6.005 ± 0.022

6.226 ± 0.014

1.062 ± 0.005

3.022 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski