Amino acid dipepetide frequency for Citrobacter phage SH4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.002AlaAla: 9.002 ± 1.078
0.73AlaCys: 0.73 ± 0.232
5.596AlaAsp: 5.596 ± 0.87
5.92AlaGlu: 5.92 ± 0.7
2.919AlaPhe: 2.919 ± 0.337
8.434AlaGly: 8.434 ± 0.935
1.054AlaHis: 1.054 ± 0.312
5.758AlaIle: 5.758 ± 0.604
7.055AlaLys: 7.055 ± 0.637
7.218AlaLeu: 7.218 ± 0.945
2.595AlaMet: 2.595 ± 0.417
3.406AlaAsn: 3.406 ± 0.448
2.109AlaPro: 2.109 ± 0.446
2.595AlaGln: 2.595 ± 0.499
3.325AlaArg: 3.325 ± 0.5
4.704AlaSer: 4.704 ± 0.74
4.055AlaThr: 4.055 ± 0.667
6.569AlaVal: 6.569 ± 0.994
1.216AlaTrp: 1.216 ± 0.322
2.352AlaTyr: 2.352 ± 0.578
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.224
0.0CysCys: 0.0 ± 0.0
0.811CysAsp: 0.811 ± 0.366
0.405CysGlu: 0.405 ± 0.185
0.811CysPhe: 0.811 ± 0.274
0.811CysGly: 0.811 ± 0.303
0.405CysHis: 0.405 ± 0.191
0.243CysIle: 0.243 ± 0.15
0.568CysLys: 0.568 ± 0.229
1.135CysLeu: 1.135 ± 0.289
0.243CysMet: 0.243 ± 0.2
0.162CysAsn: 0.162 ± 0.126
0.487CysPro: 0.487 ± 0.2
0.324CysGln: 0.324 ± 0.187
0.649CysArg: 0.649 ± 0.277
0.324CysSer: 0.324 ± 0.188
0.405CysThr: 0.405 ± 0.2
0.487CysVal: 0.487 ± 0.218
0.162CysTrp: 0.162 ± 0.177
0.162CysTyr: 0.162 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
6.569AspAla: 6.569 ± 0.721
0.892AspCys: 0.892 ± 0.502
4.055AspAsp: 4.055 ± 0.669
4.379AspGlu: 4.379 ± 0.431
2.027AspPhe: 2.027 ± 0.513
6.569AspGly: 6.569 ± 0.649
1.054AspHis: 1.054 ± 0.269
3.812AspIle: 3.812 ± 0.402
3.325AspLys: 3.325 ± 0.618
5.028AspLeu: 5.028 ± 0.562
1.784AspMet: 1.784 ± 0.334
2.109AspAsn: 2.109 ± 0.494
2.676AspPro: 2.676 ± 0.488
2.19AspGln: 2.19 ± 0.403
2.676AspArg: 2.676 ± 0.429
3.082AspSer: 3.082 ± 0.333
3.487AspThr: 3.487 ± 0.603
4.055AspVal: 4.055 ± 0.511
1.135AspTrp: 1.135 ± 0.374
2.433AspTyr: 2.433 ± 0.448
0.0AspXaa: 0.0 ± 0.0
Glu
6.974GluAla: 6.974 ± 0.956
0.73GluCys: 0.73 ± 0.32
4.704GluAsp: 4.704 ± 0.738
4.298GluGlu: 4.298 ± 0.747
2.271GluPhe: 2.271 ± 0.439
5.596GluGly: 5.596 ± 0.737
0.811GluHis: 0.811 ± 0.256
2.838GluIle: 2.838 ± 0.39
3.082GluLys: 3.082 ± 0.494
5.433GluLeu: 5.433 ± 0.671
1.946GluMet: 1.946 ± 0.445
2.352GluAsn: 2.352 ± 0.486
2.352GluPro: 2.352 ± 0.442
3.568GluGln: 3.568 ± 0.548
4.136GluArg: 4.136 ± 0.52
3.568GluSer: 3.568 ± 0.568
3.812GluThr: 3.812 ± 0.531
4.217GluVal: 4.217 ± 0.675
1.298GluTrp: 1.298 ± 0.323
2.838GluTyr: 2.838 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
2.676PheAla: 2.676 ± 0.548
0.324PheCys: 0.324 ± 0.188
2.757PheAsp: 2.757 ± 0.481
1.784PheGlu: 1.784 ± 0.399
1.216PhePhe: 1.216 ± 0.38
2.514PheGly: 2.514 ± 0.589
0.811PheHis: 0.811 ± 0.225
1.784PheIle: 1.784 ± 0.422
2.676PheLys: 2.676 ± 0.513
3.325PheLeu: 3.325 ± 0.447
0.892PheMet: 0.892 ± 0.263
2.595PheAsn: 2.595 ± 0.394
1.541PhePro: 1.541 ± 0.424
0.649PheGln: 0.649 ± 0.254
1.784PheArg: 1.784 ± 0.339
2.352PheSer: 2.352 ± 0.377
2.514PheThr: 2.514 ± 0.393
2.838PheVal: 2.838 ± 0.596
0.162PheTrp: 0.162 ± 0.097
1.216PheTyr: 1.216 ± 0.307
0.0PheXaa: 0.0 ± 0.0
Gly
6.326GlyAla: 6.326 ± 0.796
0.649GlyCys: 0.649 ± 0.292
5.028GlyAsp: 5.028 ± 0.926
5.352GlyGlu: 5.352 ± 0.667
1.946GlyPhe: 1.946 ± 0.337
5.433GlyGly: 5.433 ± 0.855
0.811GlyHis: 0.811 ± 0.256
3.73GlyIle: 3.73 ± 0.585
7.38GlyLys: 7.38 ± 0.809
6.812GlyLeu: 6.812 ± 0.807
2.757GlyMet: 2.757 ± 0.62
2.595GlyAsn: 2.595 ± 0.481
1.541GlyPro: 1.541 ± 0.443
3.244GlyGln: 3.244 ± 0.558
5.433GlyArg: 5.433 ± 0.467
6.488GlySer: 6.488 ± 0.656
4.379GlyThr: 4.379 ± 0.733
4.704GlyVal: 4.704 ± 0.679
1.622GlyTrp: 1.622 ± 0.341
4.298GlyTyr: 4.298 ± 0.651
0.0GlyXaa: 0.0 ± 0.0
His
0.649HisAla: 0.649 ± 0.258
0.243HisCys: 0.243 ± 0.156
1.298HisAsp: 1.298 ± 0.451
1.054HisGlu: 1.054 ± 0.253
0.324HisPhe: 0.324 ± 0.175
0.973HisGly: 0.973 ± 0.299
0.324HisHis: 0.324 ± 0.148
1.054HisIle: 1.054 ± 0.286
0.973HisLys: 0.973 ± 0.238
1.865HisLeu: 1.865 ± 0.412
0.487HisMet: 0.487 ± 0.198
0.568HisAsn: 0.568 ± 0.216
0.649HisPro: 0.649 ± 0.197
0.487HisGln: 0.487 ± 0.204
0.73HisArg: 0.73 ± 0.186
0.892HisSer: 0.892 ± 0.283
1.46HisThr: 1.46 ± 0.42
0.892HisVal: 0.892 ± 0.263
0.568HisTrp: 0.568 ± 0.239
0.568HisTyr: 0.568 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
3.893IleAla: 3.893 ± 0.603
0.73IleCys: 0.73 ± 0.349
3.244IleAsp: 3.244 ± 0.416
2.676IleGlu: 2.676 ± 0.381
0.811IlePhe: 0.811 ± 0.249
4.46IleGly: 4.46 ± 0.504
1.216IleHis: 1.216 ± 0.456
1.865IleIle: 1.865 ± 0.318
3.487IleLys: 3.487 ± 0.632
3.406IleLeu: 3.406 ± 0.565
1.216IleMet: 1.216 ± 0.381
2.838IleAsn: 2.838 ± 0.658
2.109IlePro: 2.109 ± 0.433
2.027IleGln: 2.027 ± 0.534
2.919IleArg: 2.919 ± 0.532
2.271IleSer: 2.271 ± 0.425
3.082IleThr: 3.082 ± 0.349
3.893IleVal: 3.893 ± 0.597
0.649IleTrp: 0.649 ± 0.201
1.379IleTyr: 1.379 ± 0.362
0.0IleXaa: 0.0 ± 0.0
Lys
6.731LysAla: 6.731 ± 0.657
0.892LysCys: 0.892 ± 0.331
3.406LysAsp: 3.406 ± 0.436
4.055LysGlu: 4.055 ± 0.613
2.757LysPhe: 2.757 ± 0.43
4.298LysGly: 4.298 ± 0.64
1.135LysHis: 1.135 ± 0.358
1.946LysIle: 1.946 ± 0.422
3.893LysLys: 3.893 ± 0.816
5.109LysLeu: 5.109 ± 0.568
2.19LysMet: 2.19 ± 0.354
2.757LysAsn: 2.757 ± 0.41
2.757LysPro: 2.757 ± 0.555
2.352LysGln: 2.352 ± 0.463
3.73LysArg: 3.73 ± 0.531
5.028LysSer: 5.028 ± 0.585
3.73LysThr: 3.73 ± 0.479
5.028LysVal: 5.028 ± 0.658
1.135LysTrp: 1.135 ± 0.304
2.433LysTyr: 2.433 ± 0.472
0.0LysXaa: 0.0 ± 0.0
Leu
7.218LeuAla: 7.218 ± 0.925
0.324LeuCys: 0.324 ± 0.196
4.704LeuAsp: 4.704 ± 0.483
6.488LeuGlu: 6.488 ± 0.693
2.838LeuPhe: 2.838 ± 0.442
5.028LeuGly: 5.028 ± 0.545
1.054LeuHis: 1.054 ± 0.33
3.487LeuIle: 3.487 ± 0.531
7.055LeuLys: 7.055 ± 0.943
6.001LeuLeu: 6.001 ± 0.89
2.595LeuMet: 2.595 ± 0.383
4.217LeuAsn: 4.217 ± 0.658
3.568LeuPro: 3.568 ± 0.364
3.974LeuGln: 3.974 ± 0.564
5.109LeuArg: 5.109 ± 0.459
5.515LeuSer: 5.515 ± 0.677
5.677LeuThr: 5.677 ± 0.997
5.758LeuVal: 5.758 ± 0.711
0.73LeuTrp: 0.73 ± 0.284
2.19LeuTyr: 2.19 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
3.325MetAla: 3.325 ± 0.457
0.405MetCys: 0.405 ± 0.214
1.46MetAsp: 1.46 ± 0.366
2.027MetGlu: 2.027 ± 0.312
0.892MetPhe: 0.892 ± 0.269
2.271MetGly: 2.271 ± 0.43
0.243MetHis: 0.243 ± 0.121
1.216MetIle: 1.216 ± 0.301
1.054MetLys: 1.054 ± 0.254
2.595MetLeu: 2.595 ± 0.423
0.73MetMet: 0.73 ± 0.213
1.298MetAsn: 1.298 ± 0.342
0.973MetPro: 0.973 ± 0.352
0.973MetGln: 0.973 ± 0.259
1.46MetArg: 1.46 ± 0.362
1.865MetSer: 1.865 ± 0.406
2.19MetThr: 2.19 ± 0.362
2.676MetVal: 2.676 ± 0.463
0.324MetTrp: 0.324 ± 0.167
0.811MetTyr: 0.811 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
3.893AsnAla: 3.893 ± 0.59
0.487AsnCys: 0.487 ± 0.195
2.757AsnAsp: 2.757 ± 0.526
2.109AsnGlu: 2.109 ± 0.395
1.946AsnPhe: 1.946 ± 0.293
3.649AsnGly: 3.649 ± 0.546
0.568AsnHis: 0.568 ± 0.205
2.109AsnIle: 2.109 ± 0.458
2.109AsnLys: 2.109 ± 0.355
3.649AsnLeu: 3.649 ± 0.609
1.054AsnMet: 1.054 ± 0.271
1.703AsnAsn: 1.703 ± 0.427
2.919AsnPro: 2.919 ± 0.435
1.541AsnGln: 1.541 ± 0.289
2.352AsnArg: 2.352 ± 0.517
2.676AsnSer: 2.676 ± 0.578
2.027AsnThr: 2.027 ± 0.497
2.514AsnVal: 2.514 ± 0.432
0.243AsnTrp: 0.243 ± 0.175
1.298AsnTyr: 1.298 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
2.838ProAla: 2.838 ± 0.493
0.405ProCys: 0.405 ± 0.213
1.784ProAsp: 1.784 ± 0.286
3.406ProGlu: 3.406 ± 0.558
1.703ProPhe: 1.703 ± 0.319
1.946ProGly: 1.946 ± 0.3
0.649ProHis: 0.649 ± 0.229
1.865ProIle: 1.865 ± 0.389
2.757ProLys: 2.757 ± 0.668
2.595ProLeu: 2.595 ± 0.433
1.135ProMet: 1.135 ± 0.34
2.433ProAsn: 2.433 ± 0.401
1.054ProPro: 1.054 ± 0.361
1.784ProGln: 1.784 ± 0.417
1.703ProArg: 1.703 ± 0.362
2.433ProSer: 2.433 ± 0.312
3.244ProThr: 3.244 ± 0.443
3.163ProVal: 3.163 ± 0.554
0.811ProTrp: 0.811 ± 0.268
1.135ProTyr: 1.135 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.73GlnAla: 3.73 ± 0.589
0.0GlnCys: 0.0 ± 0.0
3.649GlnAsp: 3.649 ± 0.725
2.514GlnGlu: 2.514 ± 0.5
1.946GlnPhe: 1.946 ± 0.371
2.919GlnGly: 2.919 ± 0.456
0.568GlnHis: 0.568 ± 0.248
1.379GlnIle: 1.379 ± 0.322
1.946GlnLys: 1.946 ± 0.437
5.028GlnLeu: 5.028 ± 0.833
1.135GlnMet: 1.135 ± 0.274
1.703GlnAsn: 1.703 ± 0.444
1.298GlnPro: 1.298 ± 0.38
1.865GlnGln: 1.865 ± 0.478
2.676GlnArg: 2.676 ± 0.661
2.676GlnSer: 2.676 ± 0.527
2.271GlnThr: 2.271 ± 0.466
2.19GlnVal: 2.19 ± 0.331
0.73GlnTrp: 0.73 ± 0.198
1.054GlnTyr: 1.054 ± 0.425
0.0GlnXaa: 0.0 ± 0.0
Arg
4.785ArgAla: 4.785 ± 0.765
0.649ArgCys: 0.649 ± 0.253
4.298ArgAsp: 4.298 ± 0.453
3.974ArgGlu: 3.974 ± 0.527
2.838ArgPhe: 2.838 ± 0.408
3.974ArgGly: 3.974 ± 0.465
0.811ArgHis: 0.811 ± 0.243
3.406ArgIle: 3.406 ± 0.62
3.163ArgLys: 3.163 ± 0.6
5.758ArgLeu: 5.758 ± 0.724
1.135ArgMet: 1.135 ± 0.413
1.865ArgAsn: 1.865 ± 0.339
1.865ArgPro: 1.865 ± 0.346
2.595ArgGln: 2.595 ± 0.501
1.865ArgArg: 1.865 ± 0.372
3.325ArgSer: 3.325 ± 0.513
2.027ArgThr: 2.027 ± 0.395
3.244ArgVal: 3.244 ± 0.559
1.216ArgTrp: 1.216 ± 0.347
1.379ArgTyr: 1.379 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
4.947SerAla: 4.947 ± 0.743
0.73SerCys: 0.73 ± 0.249
4.785SerAsp: 4.785 ± 0.507
3.244SerGlu: 3.244 ± 0.515
2.757SerPhe: 2.757 ± 0.503
6.812SerGly: 6.812 ± 1.242
1.946SerHis: 1.946 ± 0.448
2.352SerIle: 2.352 ± 0.431
3.406SerLys: 3.406 ± 0.477
3.568SerLeu: 3.568 ± 0.562
1.784SerMet: 1.784 ± 0.463
1.703SerAsn: 1.703 ± 0.352
3.001SerPro: 3.001 ± 0.583
2.352SerGln: 2.352 ± 0.507
3.001SerArg: 3.001 ± 0.473
3.893SerSer: 3.893 ± 0.653
3.568SerThr: 3.568 ± 0.595
4.136SerVal: 4.136 ± 0.472
0.973SerTrp: 0.973 ± 0.189
2.514SerTyr: 2.514 ± 0.469
0.0SerXaa: 0.0 ± 0.0
Thr
3.406ThrAla: 3.406 ± 0.716
0.243ThrCys: 0.243 ± 0.137
2.757ThrAsp: 2.757 ± 0.473
5.19ThrGlu: 5.19 ± 0.555
2.19ThrPhe: 2.19 ± 0.431
5.677ThrGly: 5.677 ± 0.738
0.405ThrHis: 0.405 ± 0.182
3.649ThrIle: 3.649 ± 0.585
3.568ThrLys: 3.568 ± 0.543
5.19ThrLeu: 5.19 ± 0.803
1.46ThrMet: 1.46 ± 0.342
1.946ThrAsn: 1.946 ± 0.486
3.325ThrPro: 3.325 ± 0.39
2.109ThrGln: 2.109 ± 0.426
3.082ThrArg: 3.082 ± 0.431
2.595ThrSer: 2.595 ± 0.596
3.406ThrThr: 3.406 ± 0.539
4.947ThrVal: 4.947 ± 0.899
0.973ThrTrp: 0.973 ± 0.313
1.541ThrTyr: 1.541 ± 0.344
0.0ThrXaa: 0.0 ± 0.0
Val
4.785ValAla: 4.785 ± 0.597
0.487ValCys: 0.487 ± 0.22
3.568ValAsp: 3.568 ± 0.545
5.19ValGlu: 5.19 ± 0.609
2.352ValPhe: 2.352 ± 0.588
4.947ValGly: 4.947 ± 0.544
1.379ValHis: 1.379 ± 0.49
3.73ValIle: 3.73 ± 0.476
4.866ValLys: 4.866 ± 0.705
5.109ValLeu: 5.109 ± 0.626
2.027ValMet: 2.027 ± 0.45
2.676ValAsn: 2.676 ± 0.673
2.838ValPro: 2.838 ± 0.452
4.055ValGln: 4.055 ± 0.686
4.379ValArg: 4.379 ± 0.672
5.028ValSer: 5.028 ± 0.863
3.812ValThr: 3.812 ± 0.552
6.082ValVal: 6.082 ± 1.057
0.892ValTrp: 0.892 ± 0.29
2.514ValTyr: 2.514 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
0.487TrpAla: 0.487 ± 0.192
0.162TrpCys: 0.162 ± 0.119
0.568TrpAsp: 0.568 ± 0.181
1.054TrpGlu: 1.054 ± 0.245
0.73TrpPhe: 0.73 ± 0.274
1.379TrpGly: 1.379 ± 0.354
0.405TrpHis: 0.405 ± 0.152
0.487TrpIle: 0.487 ± 0.183
1.135TrpLys: 1.135 ± 0.385
2.19TrpLeu: 2.19 ± 0.356
0.243TrpMet: 0.243 ± 0.145
1.054TrpAsn: 1.054 ± 0.274
0.243TrpPro: 0.243 ± 0.137
0.811TrpGln: 0.811 ± 0.268
0.892TrpArg: 0.892 ± 0.256
1.135TrpSer: 1.135 ± 0.453
0.649TrpThr: 0.649 ± 0.247
1.298TrpVal: 1.298 ± 0.321
0.162TrpTrp: 0.162 ± 0.109
0.568TrpTyr: 0.568 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.649TyrAla: 3.649 ± 0.609
0.243TyrCys: 0.243 ± 0.153
2.109TyrAsp: 2.109 ± 0.485
1.703TyrGlu: 1.703 ± 0.414
1.216TyrPhe: 1.216 ± 0.333
2.838TyrGly: 2.838 ± 0.541
0.405TyrHis: 0.405 ± 0.172
1.46TyrIle: 1.46 ± 0.467
1.865TyrLys: 1.865 ± 0.326
2.514TyrLeu: 2.514 ± 0.448
1.298TyrMet: 1.298 ± 0.243
1.622TyrAsn: 1.622 ± 0.355
1.541TyrPro: 1.541 ± 0.373
1.784TyrGln: 1.784 ± 0.562
2.271TyrArg: 2.271 ± 0.504
1.622TyrSer: 1.622 ± 0.355
1.865TyrThr: 1.865 ± 0.389
2.027TyrVal: 2.027 ± 0.375
0.649TyrTrp: 0.649 ± 0.251
0.973TyrTyr: 0.973 ± 0.214
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski