West African Asystasia virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9CM36|G9CM36_9GEMI AC4 protein OS=West African Asystasia virus 2 OX=1046574 GN=AC4 PE=3 SV=1
MM1 pKa = 7.65APPKK5 pKa = 10.4RR6 pKa = 11.84FLINCKK12 pKa = 10.28NYY14 pKa = 9.78FLTYY18 pKa = 8.41PQCPLTKK25 pKa = 10.33EE26 pKa = 3.99EE27 pKa = 4.77ALSQIINLSTPTNKK41 pKa = 10.01LFIKK45 pKa = 10.0ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.41PHH57 pKa = 7.12LHH59 pKa = 6.03MLVQFEE65 pKa = 5.29GKK67 pKa = 9.97FKK69 pKa = 10.6CQNKK73 pKa = 9.52RR74 pKa = 11.84FFDD77 pKa = 3.9LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.83IEE105 pKa = 4.16KK106 pKa = 10.94DD107 pKa = 3.04GDD109 pKa = 3.7TLEE112 pKa = 4.34WGTFQIDD119 pKa = 3.03GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQNANDD132 pKa = 4.04ACAEE136 pKa = 3.94ALNAGSADD144 pKa = 3.29AALAIIRR151 pKa = 11.84EE152 pKa = 4.23KK153 pKa = 10.87LPKK156 pKa = 10.46DD157 pKa = 4.77FIFQYY162 pKa = 11.18HH163 pKa = 5.38NLKK166 pKa = 10.92SNLDD170 pKa = 4.37RR171 pKa = 11.84IFMSPPEE178 pKa = 4.46AYY180 pKa = 9.95VSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.87EE195 pKa = 4.25LEE197 pKa = 4.08VWVSEE202 pKa = 4.06NVMGPAARR210 pKa = 11.84PWRR213 pKa = 11.84PNSIVIEE220 pKa = 4.34GDD222 pKa = 3.08SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.4FMGAQRR279 pKa = 11.84NWNSNVKK286 pKa = 9.8YY287 pKa = 10.24GKK289 pKa = 9.2PVQIKK294 pKa = 10.41GGIPTIFLCNPGPTSSYY311 pKa = 10.9KK312 pKa = 10.53EE313 pKa = 3.85YY314 pKa = 11.17LEE316 pKa = 4.21EE317 pKa = 4.38EE318 pKa = 4.59KK319 pKa = 11.1NSSLKK324 pKa = 9.25TWAIKK329 pKa = 9.98NASFITLVGPLYY341 pKa = 10.93SGANQSQPQTRR352 pKa = 11.84EE353 pKa = 3.86TQTNPQEE360 pKa = 3.98EE361 pKa = 4.57DD362 pKa = 2.96

Molecular weight: 40.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9CM34|G9CM34_9GEMI Transcriptional activator protein OS=West African Asystasia virus 2 OX=1046574 GN=AC2 PE=3 SV=1
MM1 pKa = 8.51DD2 pKa = 4.64SRR4 pKa = 11.84TEE6 pKa = 3.97EE7 pKa = 4.41NITAAQAKK15 pKa = 8.49NGVYY19 pKa = 9.4IWEE22 pKa = 4.4ISNPLYY28 pKa = 10.66FKK30 pKa = 10.6ILNHH34 pKa = 6.29DD35 pKa = 4.05VRR37 pKa = 11.84PMSRR41 pKa = 11.84PWDD44 pKa = 3.64IIKK47 pKa = 10.04IQVRR51 pKa = 11.84FNHH54 pKa = 6.14NLRR57 pKa = 11.84KK58 pKa = 9.81ALGIHH63 pKa = 6.15RR64 pKa = 11.84CLLTFQAWTSLRR76 pKa = 11.84PQTSHH81 pKa = 7.05FLRR84 pKa = 11.84VFSKK88 pKa = 10.83HH89 pKa = 4.94VIRR92 pKa = 11.84YY93 pKa = 8.0LNSLGVLSINNLIRR107 pKa = 11.84AIKK110 pKa = 9.31FVLYY114 pKa = 10.53NVLHH118 pKa = 5.62GTT120 pKa = 3.73

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1075

85

362

179.2

20.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.395 ± 0.782

2.512 ± 0.499

4.651 ± 0.399

4.744 ± 0.864

4.279 ± 0.395

5.209 ± 0.575

4.0 ± 0.653

5.488 ± 0.845

6.047 ± 0.615

7.814 ± 0.734

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.68

6.233 ± 0.503

6.419 ± 0.641

4.372 ± 0.555

6.791 ± 1.165

7.814 ± 1.132

5.116 ± 0.268

5.209 ± 0.79

1.395 ± 0.282

3.628 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski