Amino acid dipepetide frequency for Paenibacillus phage PBL1c

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.413AlaAla: 5.413 ± 0.987
0.796AlaCys: 0.796 ± 0.186
4.378AlaAsp: 4.378 ± 0.675
6.13AlaGlu: 6.13 ± 0.777
2.07AlaPhe: 2.07 ± 0.386
4.139AlaGly: 4.139 ± 0.65
1.035AlaHis: 1.035 ± 0.272
4.697AlaIle: 4.697 ± 0.846
5.652AlaLys: 5.652 ± 0.744
5.572AlaLeu: 5.572 ± 0.779
1.194AlaMet: 1.194 ± 0.263
2.866AlaAsn: 2.866 ± 0.444
1.512AlaPro: 1.512 ± 0.311
2.547AlaGln: 2.547 ± 0.423
2.149AlaArg: 2.149 ± 0.421
4.299AlaSer: 4.299 ± 0.795
3.741AlaThr: 3.741 ± 0.779
3.821AlaVal: 3.821 ± 0.542
1.433AlaTrp: 1.433 ± 0.329
2.468AlaTyr: 2.468 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.318CysAla: 0.318 ± 0.159
0.239CysCys: 0.239 ± 0.133
0.637CysAsp: 0.637 ± 0.219
0.557CysGlu: 0.557 ± 0.251
0.398CysPhe: 0.398 ± 0.229
0.955CysGly: 0.955 ± 0.258
0.239CysHis: 0.239 ± 0.153
0.955CysIle: 0.955 ± 0.279
1.353CysLys: 1.353 ± 0.377
0.955CysLeu: 0.955 ± 0.247
0.08CysMet: 0.08 ± 0.079
0.398CysAsn: 0.398 ± 0.193
0.557CysPro: 0.557 ± 0.233
0.318CysGln: 0.318 ± 0.153
0.318CysArg: 0.318 ± 0.166
0.796CysSer: 0.796 ± 0.257
0.239CysThr: 0.239 ± 0.14
0.478CysVal: 0.478 ± 0.211
0.239CysTrp: 0.239 ± 0.19
0.239CysTyr: 0.239 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
3.98AspAla: 3.98 ± 0.551
0.478AspCys: 0.478 ± 0.193
4.299AspAsp: 4.299 ± 0.707
4.139AspGlu: 4.139 ± 0.807
3.343AspPhe: 3.343 ± 0.492
3.662AspGly: 3.662 ± 0.578
0.796AspHis: 0.796 ± 0.245
3.184AspIle: 3.184 ± 0.398
3.98AspLys: 3.98 ± 0.572
5.334AspLeu: 5.334 ± 0.656
2.229AspMet: 2.229 ± 0.419
2.309AspAsn: 2.309 ± 0.403
1.831AspPro: 1.831 ± 0.354
2.468AspGln: 2.468 ± 0.454
2.627AspArg: 2.627 ± 0.449
2.945AspSer: 2.945 ± 0.425
2.866AspThr: 2.866 ± 0.487
3.741AspVal: 3.741 ± 0.56
0.876AspTrp: 0.876 ± 0.279
2.07AspTyr: 2.07 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
5.732GluAla: 5.732 ± 0.707
0.318GluCys: 0.318 ± 0.146
5.015GluAsp: 5.015 ± 0.772
6.528GluGlu: 6.528 ± 0.974
2.627GluPhe: 2.627 ± 0.409
3.901GluGly: 3.901 ± 0.532
1.353GluHis: 1.353 ± 0.412
6.607GluIle: 6.607 ± 0.814
8.279GluLys: 8.279 ± 0.901
7.483GluLeu: 7.483 ± 0.939
4.458GluMet: 4.458 ± 0.481
3.741GluAsn: 3.741 ± 0.627
2.149GluPro: 2.149 ± 0.406
4.139GluGln: 4.139 ± 0.745
4.219GluArg: 4.219 ± 0.616
3.662GluSer: 3.662 ± 0.495
4.378GluThr: 4.378 ± 0.521
6.13GluVal: 6.13 ± 0.868
0.955GluTrp: 0.955 ± 0.292
2.945GluTyr: 2.945 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
1.911PheAla: 1.911 ± 0.39
1.114PheCys: 1.114 ± 0.306
2.468PheAsp: 2.468 ± 0.447
3.662PheGlu: 3.662 ± 0.602
0.876PhePhe: 0.876 ± 0.281
2.07PheGly: 2.07 ± 0.435
1.035PheHis: 1.035 ± 0.306
3.105PheIle: 3.105 ± 0.499
2.547PheLys: 2.547 ± 0.582
2.388PheLeu: 2.388 ± 0.511
0.796PheMet: 0.796 ± 0.287
2.149PheAsn: 2.149 ± 0.383
0.557PhePro: 0.557 ± 0.239
1.433PheGln: 1.433 ± 0.314
1.114PheArg: 1.114 ± 0.298
2.786PheSer: 2.786 ± 0.47
1.911PheThr: 1.911 ± 0.339
2.707PheVal: 2.707 ± 0.466
0.478PheTrp: 0.478 ± 0.226
1.274PheTyr: 1.274 ± 0.339
0.0PheXaa: 0.0 ± 0.0
Gly
3.264GlyAla: 3.264 ± 0.604
0.955GlyCys: 0.955 ± 0.248
2.945GlyAsp: 2.945 ± 0.507
5.254GlyGlu: 5.254 ± 0.744
2.07GlyPhe: 2.07 ± 0.415
3.343GlyGly: 3.343 ± 0.621
0.796GlyHis: 0.796 ± 0.257
4.936GlyIle: 4.936 ± 0.943
6.528GlyLys: 6.528 ± 0.631
4.537GlyLeu: 4.537 ± 0.835
1.831GlyMet: 1.831 ± 0.623
3.105GlyAsn: 3.105 ± 0.537
0.796GlyPro: 0.796 ± 0.248
2.309GlyGln: 2.309 ± 0.437
3.582GlyArg: 3.582 ± 0.555
3.264GlySer: 3.264 ± 0.682
2.547GlyThr: 2.547 ± 0.584
3.582GlyVal: 3.582 ± 0.536
1.114GlyTrp: 1.114 ± 0.25
2.547GlyTyr: 2.547 ± 0.421
0.0GlyXaa: 0.0 ± 0.0
His
1.274HisAla: 1.274 ± 0.303
0.398HisCys: 0.398 ± 0.183
0.557HisAsp: 0.557 ± 0.225
1.433HisGlu: 1.433 ± 0.37
0.637HisPhe: 0.637 ± 0.266
0.955HisGly: 0.955 ± 0.308
0.637HisHis: 0.637 ± 0.204
1.751HisIle: 1.751 ± 0.38
1.194HisLys: 1.194 ± 0.311
1.99HisLeu: 1.99 ± 0.358
0.478HisMet: 0.478 ± 0.214
0.796HisAsn: 0.796 ± 0.252
0.955HisPro: 0.955 ± 0.287
0.398HisGln: 0.398 ± 0.174
1.274HisArg: 1.274 ± 0.286
0.796HisSer: 0.796 ± 0.249
0.478HisThr: 0.478 ± 0.237
1.194HisVal: 1.194 ± 0.252
0.318HisTrp: 0.318 ± 0.141
0.557HisTyr: 0.557 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.139IleAla: 4.139 ± 0.65
0.796IleCys: 0.796 ± 0.308
4.776IleAsp: 4.776 ± 0.695
6.846IleGlu: 6.846 ± 0.774
3.105IlePhe: 3.105 ± 0.493
3.582IleGly: 3.582 ± 0.703
1.672IleHis: 1.672 ± 0.364
2.945IleIle: 2.945 ± 0.739
7.403IleLys: 7.403 ± 1.083
5.572IleLeu: 5.572 ± 0.598
1.831IleMet: 1.831 ± 0.383
3.901IleAsn: 3.901 ± 0.521
2.866IlePro: 2.866 ± 0.4
3.105IleGln: 3.105 ± 0.465
3.741IleArg: 3.741 ± 0.705
3.582IleSer: 3.582 ± 0.669
4.06IleThr: 4.06 ± 0.53
5.015IleVal: 5.015 ± 0.665
0.557IleTrp: 0.557 ± 0.252
2.707IleTyr: 2.707 ± 0.445
0.0IleXaa: 0.0 ± 0.0
Lys
6.448LysAla: 6.448 ± 0.731
0.398LysCys: 0.398 ± 0.164
4.617LysAsp: 4.617 ± 0.49
9.553LysGlu: 9.553 ± 0.865
2.309LysPhe: 2.309 ± 0.312
5.413LysGly: 5.413 ± 0.526
1.433LysHis: 1.433 ± 0.294
5.572LysIle: 5.572 ± 0.485
8.757LysLys: 8.757 ± 1.027
5.97LysLeu: 5.97 ± 0.564
3.105LysMet: 3.105 ± 0.471
4.06LysAsn: 4.06 ± 0.68
2.786LysPro: 2.786 ± 0.501
4.458LysGln: 4.458 ± 0.646
5.97LysArg: 5.97 ± 0.668
5.254LysSer: 5.254 ± 0.645
5.572LysThr: 5.572 ± 0.711
5.015LysVal: 5.015 ± 0.571
1.194LysTrp: 1.194 ± 0.255
3.105LysTyr: 3.105 ± 0.472
0.0LysXaa: 0.0 ± 0.0
Leu
6.289LeuAla: 6.289 ± 0.681
1.035LeuCys: 1.035 ± 0.3
4.299LeuAsp: 4.299 ± 0.679
6.528LeuGlu: 6.528 ± 0.766
3.105LeuPhe: 3.105 ± 0.651
4.299LeuGly: 4.299 ± 0.467
1.911LeuHis: 1.911 ± 0.412
5.572LeuIle: 5.572 ± 0.504
6.846LeuLys: 6.846 ± 0.667
4.776LeuLeu: 4.776 ± 0.683
1.831LeuMet: 1.831 ± 0.408
3.901LeuAsn: 3.901 ± 0.481
3.264LeuPro: 3.264 ± 0.491
3.821LeuGln: 3.821 ± 0.528
3.503LeuArg: 3.503 ± 0.46
5.732LeuSer: 5.732 ± 0.594
4.856LeuThr: 4.856 ± 0.632
3.662LeuVal: 3.662 ± 0.456
0.876LeuTrp: 0.876 ± 0.329
3.025LeuTyr: 3.025 ± 0.504
0.0LeuXaa: 0.0 ± 0.0
Met
1.911MetAla: 1.911 ± 0.356
0.08MetCys: 0.08 ± 0.078
1.433MetAsp: 1.433 ± 0.319
2.627MetGlu: 2.627 ± 0.399
1.035MetPhe: 1.035 ± 0.211
1.433MetGly: 1.433 ± 0.343
0.239MetHis: 0.239 ± 0.127
2.707MetIle: 2.707 ± 0.489
3.821MetLys: 3.821 ± 0.536
2.627MetLeu: 2.627 ± 0.462
0.398MetMet: 0.398 ± 0.203
2.229MetAsn: 2.229 ± 0.413
1.194MetPro: 1.194 ± 0.334
1.274MetGln: 1.274 ± 0.273
1.114MetArg: 1.114 ± 0.294
2.229MetSer: 2.229 ± 0.351
1.353MetThr: 1.353 ± 0.309
1.274MetVal: 1.274 ± 0.292
0.318MetTrp: 0.318 ± 0.141
1.035MetTyr: 1.035 ± 0.314
0.0MetXaa: 0.0 ± 0.0
Asn
2.547AsnAla: 2.547 ± 0.398
0.398AsnCys: 0.398 ± 0.178
1.99AsnAsp: 1.99 ± 0.494
4.299AsnGlu: 4.299 ± 0.736
1.433AsnPhe: 1.433 ± 0.364
4.06AsnGly: 4.06 ± 0.576
0.796AsnHis: 0.796 ± 0.255
3.264AsnIle: 3.264 ± 0.546
3.901AsnLys: 3.901 ± 0.497
3.901AsnLeu: 3.901 ± 0.481
1.592AsnMet: 1.592 ± 0.426
1.99AsnAsn: 1.99 ± 0.414
2.309AsnPro: 2.309 ± 0.566
2.149AsnGln: 2.149 ± 0.432
3.582AsnArg: 3.582 ± 0.712
2.866AsnSer: 2.866 ± 0.469
2.309AsnThr: 2.309 ± 0.443
2.866AsnVal: 2.866 ± 0.428
0.478AsnTrp: 0.478 ± 0.203
1.751AsnTyr: 1.751 ± 0.411
0.0AsnXaa: 0.0 ± 0.0
Pro
2.149ProAla: 2.149 ± 0.361
0.159ProCys: 0.159 ± 0.111
1.353ProAsp: 1.353 ± 0.347
2.945ProGlu: 2.945 ± 0.713
1.274ProPhe: 1.274 ± 0.315
2.229ProGly: 2.229 ± 0.447
0.637ProHis: 0.637 ± 0.257
2.309ProIle: 2.309 ± 0.433
3.025ProLys: 3.025 ± 0.536
2.309ProLeu: 2.309 ± 0.496
0.637ProMet: 0.637 ± 0.22
1.911ProAsn: 1.911 ± 0.432
1.035ProPro: 1.035 ± 0.288
0.876ProGln: 0.876 ± 0.27
1.194ProArg: 1.194 ± 0.311
2.388ProSer: 2.388 ± 0.642
1.672ProThr: 1.672 ± 0.302
1.672ProVal: 1.672 ± 0.472
0.478ProTrp: 0.478 ± 0.183
1.353ProTyr: 1.353 ± 0.313
0.0ProXaa: 0.0 ± 0.0
Gln
3.184GlnAla: 3.184 ± 0.544
0.08GlnCys: 0.08 ± 0.09
1.911GlnAsp: 1.911 ± 0.323
3.662GlnGlu: 3.662 ± 0.561
1.751GlnPhe: 1.751 ± 0.406
2.388GlnGly: 2.388 ± 0.477
1.035GlnHis: 1.035 ± 0.264
3.662GlnIle: 3.662 ± 0.7
3.105GlnLys: 3.105 ± 0.587
4.139GlnLeu: 4.139 ± 0.682
1.831GlnMet: 1.831 ± 0.43
1.751GlnAsn: 1.751 ± 0.303
1.751GlnPro: 1.751 ± 0.531
1.911GlnGln: 1.911 ± 0.395
2.547GlnArg: 2.547 ± 0.483
2.309GlnSer: 2.309 ± 0.372
1.911GlnThr: 1.911 ± 0.367
1.99GlnVal: 1.99 ± 0.46
0.398GlnTrp: 0.398 ± 0.221
1.274GlnTyr: 1.274 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
2.945ArgAla: 2.945 ± 0.467
0.557ArgCys: 0.557 ± 0.212
2.945ArgAsp: 2.945 ± 0.5
4.617ArgGlu: 4.617 ± 0.789
1.353ArgPhe: 1.353 ± 0.398
2.786ArgGly: 2.786 ± 0.512
0.876ArgHis: 0.876 ± 0.272
3.582ArgIle: 3.582 ± 0.526
5.811ArgLys: 5.811 ± 0.732
4.219ArgLeu: 4.219 ± 0.616
1.911ArgMet: 1.911 ± 0.38
2.707ArgAsn: 2.707 ± 0.409
1.353ArgPro: 1.353 ± 0.383
2.627ArgGln: 2.627 ± 0.515
2.866ArgArg: 2.866 ± 0.485
2.388ArgSer: 2.388 ± 0.393
2.07ArgThr: 2.07 ± 0.395
2.149ArgVal: 2.149 ± 0.395
0.796ArgTrp: 0.796 ± 0.27
1.672ArgTyr: 1.672 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
5.174SerAla: 5.174 ± 0.809
0.398SerCys: 0.398 ± 0.168
3.662SerAsp: 3.662 ± 0.501
4.378SerGlu: 4.378 ± 0.484
2.627SerPhe: 2.627 ± 0.519
3.582SerGly: 3.582 ± 0.666
0.637SerHis: 0.637 ± 0.249
6.05SerIle: 6.05 ± 0.809
4.537SerLys: 4.537 ± 0.738
4.776SerLeu: 4.776 ± 0.605
1.592SerMet: 1.592 ± 0.366
1.911SerAsn: 1.911 ± 0.355
1.831SerPro: 1.831 ± 0.311
2.786SerGln: 2.786 ± 0.542
3.025SerArg: 3.025 ± 0.503
3.662SerSer: 3.662 ± 0.635
3.343SerThr: 3.343 ± 0.495
3.741SerVal: 3.741 ± 0.699
0.398SerTrp: 0.398 ± 0.176
1.751SerTyr: 1.751 ± 0.306
0.0SerXaa: 0.0 ± 0.0
Thr
3.741ThrAla: 3.741 ± 0.747
0.478ThrCys: 0.478 ± 0.193
2.707ThrAsp: 2.707 ± 0.432
3.264ThrGlu: 3.264 ± 0.49
1.592ThrPhe: 1.592 ± 0.353
4.139ThrGly: 4.139 ± 0.595
1.114ThrHis: 1.114 ± 0.28
3.582ThrIle: 3.582 ± 0.584
4.537ThrLys: 4.537 ± 0.655
4.617ThrLeu: 4.617 ± 0.641
1.512ThrMet: 1.512 ± 0.401
2.149ThrAsn: 2.149 ± 0.379
2.388ThrPro: 2.388 ± 0.481
2.07ThrGln: 2.07 ± 0.383
2.07ThrArg: 2.07 ± 0.358
3.582ThrSer: 3.582 ± 0.533
2.627ThrThr: 2.627 ± 0.456
3.662ThrVal: 3.662 ± 0.523
0.239ThrTrp: 0.239 ± 0.112
2.627ThrTyr: 2.627 ± 0.491
0.0ThrXaa: 0.0 ± 0.0
Val
3.503ValAla: 3.503 ± 0.573
0.637ValCys: 0.637 ± 0.248
3.503ValAsp: 3.503 ± 0.57
4.299ValGlu: 4.299 ± 0.703
2.627ValPhe: 2.627 ± 0.425
2.707ValGly: 2.707 ± 0.554
0.716ValHis: 0.716 ± 0.301
3.901ValIle: 3.901 ± 0.516
5.334ValLys: 5.334 ± 0.655
3.98ValLeu: 3.98 ± 0.462
1.831ValMet: 1.831 ± 0.352
3.105ValAsn: 3.105 ± 0.647
1.433ValPro: 1.433 ± 0.309
1.831ValGln: 1.831 ± 0.386
3.105ValArg: 3.105 ± 0.519
5.174ValSer: 5.174 ± 0.592
4.219ValThr: 4.219 ± 0.552
3.582ValVal: 3.582 ± 0.507
1.99ValTrp: 1.99 ± 0.965
1.99ValTyr: 1.99 ± 0.463
0.0ValXaa: 0.0 ± 0.0
Trp
0.557TrpAla: 0.557 ± 0.202
0.239TrpCys: 0.239 ± 0.144
0.557TrpAsp: 0.557 ± 0.315
0.955TrpGlu: 0.955 ± 0.298
0.716TrpPhe: 0.716 ± 0.204
1.114TrpGly: 1.114 ± 0.26
0.637TrpHis: 0.637 ± 0.216
0.876TrpIle: 0.876 ± 0.224
1.114TrpLys: 1.114 ± 0.3
1.353TrpLeu: 1.353 ± 0.336
0.318TrpMet: 0.318 ± 0.156
1.751TrpAsn: 1.751 ± 1.069
0.159TrpPro: 0.159 ± 0.119
0.637TrpGln: 0.637 ± 0.224
0.318TrpArg: 0.318 ± 0.14
0.637TrpSer: 0.637 ± 0.238
0.239TrpThr: 0.239 ± 0.122
0.716TrpVal: 0.716 ± 0.215
0.159TrpTrp: 0.159 ± 0.122
0.796TrpTyr: 0.796 ± 0.25
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.433TyrAla: 1.433 ± 0.399
0.716TyrCys: 0.716 ± 0.289
3.105TyrAsp: 3.105 ± 0.539
2.945TyrGlu: 2.945 ± 0.488
1.433TyrPhe: 1.433 ± 0.313
2.547TyrGly: 2.547 ± 0.567
0.478TyrHis: 0.478 ± 0.199
2.945TyrIle: 2.945 ± 0.507
3.264TyrLys: 3.264 ± 0.43
2.627TyrLeu: 2.627 ± 0.51
0.796TyrMet: 0.796 ± 0.261
1.751TyrAsn: 1.751 ± 0.308
0.876TyrPro: 0.876 ± 0.29
1.433TyrGln: 1.433 ± 0.301
1.911TyrArg: 1.911 ± 0.342
1.512TyrSer: 1.512 ± 0.338
2.309TyrThr: 2.309 ± 0.465
2.547TyrVal: 2.547 ± 0.441
0.557TyrTrp: 0.557 ± 0.199
1.672TyrTyr: 1.672 ± 0.416
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (12563 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski