Roseovarius sp. TE539

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius; unclassified Roseovarius

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A365TST2|A0A365TST2_9RHOB HNH endonuclease OS=Roseovarius sp. TE539 OX=2249812 GN=DQW77_15690 PE=4 SV=1
MM1 pKa = 7.5NMKK4 pKa = 9.46TPFAATAFAVLAAPVVAGSAEE25 pKa = 4.26TPEE28 pKa = 4.56PDD30 pKa = 3.63PVISEE35 pKa = 4.25PAPVVASSPDD45 pKa = 2.89WTGFYY50 pKa = 10.67AGGQIGYY57 pKa = 10.14GDD59 pKa = 4.17VDD61 pKa = 4.69LSPGGSGDD69 pKa = 3.8DD70 pKa = 3.72VIGGIVGGYY79 pKa = 10.08DD80 pKa = 3.13HH81 pKa = 7.81DD82 pKa = 5.44LGNGWVIGGGLDD94 pKa = 3.56YY95 pKa = 11.24DD96 pKa = 3.98FADD99 pKa = 3.51ISVGGLDD106 pKa = 3.39VEE108 pKa = 4.69EE109 pKa = 4.31VFRR112 pKa = 11.84AKK114 pKa = 10.64LRR116 pKa = 11.84GGYY119 pKa = 9.92KK120 pKa = 9.58IGSGLIYY127 pKa = 9.52GTGGYY132 pKa = 9.91AWADD136 pKa = 3.27TDD138 pKa = 4.88VAGDD142 pKa = 3.65DD143 pKa = 3.65DD144 pKa = 5.75GYY146 pKa = 11.22FIGGGYY152 pKa = 7.75EE153 pKa = 3.99HH154 pKa = 7.18FVTEE158 pKa = 4.11QFTVGGEE165 pKa = 4.16VLYY168 pKa = 11.1HH169 pKa = 6.32EE170 pKa = 5.49FDD172 pKa = 3.62NFTGTTDD179 pKa = 3.38VEE181 pKa = 4.39ATTVQLRR188 pKa = 11.84GTFRR192 pKa = 11.84FF193 pKa = 3.79

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A365TNN1|A0A365TNN1_9RHOB Tnp_DDE_dom domain-containing protein OS=Roseovarius sp. TE539 OX=2249812 GN=DQW77_17475 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3470

0

3470

1097512

31

1809

316.3

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.252 ± 0.063

0.95 ± 0.013

6.26 ± 0.036

6.166 ± 0.039

3.594 ± 0.026

9.044 ± 0.044

2.219 ± 0.02

4.934 ± 0.028

2.751 ± 0.037

9.87 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.021

2.426 ± 0.022

5.213 ± 0.034

2.854 ± 0.024

7.619 ± 0.049

4.942 ± 0.027

5.401 ± 0.034

7.19 ± 0.037

1.398 ± 0.018

2.131 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski