Klebsiella phage Marfa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marfavirus; Klebsiella virus Marfa

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 279 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TQS1|A0A4Y5TQS1_9CAUD Uncharacterized protein OS=Klebsiella phage Marfa OX=2587809 GN=CPT_Marfa_069 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.56DD3 pKa = 3.23EE4 pKa = 5.04IIVLNEE10 pKa = 3.75AKK12 pKa = 10.68GEE14 pKa = 3.96EE15 pKa = 4.18DD16 pKa = 4.41LEE18 pKa = 4.29WMLYY22 pKa = 9.95EE23 pKa = 4.91FMVKK27 pKa = 10.08RR28 pKa = 11.84ANEE31 pKa = 4.09LGIDD35 pKa = 3.54WAVQNAYY42 pKa = 10.37GEE44 pKa = 4.32NEE46 pKa = 4.15VVIKK50 pKa = 10.48GVAYY54 pKa = 8.13TVQWQYY60 pKa = 11.94VGLEE64 pKa = 3.73PADD67 pKa = 3.48YY68 pKa = 10.98KK69 pKa = 10.94EE70 pKa = 4.58IYY72 pKa = 9.9NKK74 pKa = 8.7EE75 pKa = 4.24TGEE78 pKa = 3.93VDD80 pKa = 3.69YY81 pKa = 11.35DD82 pKa = 4.23PVGPWSWEE90 pKa = 3.56YY91 pKa = 11.1GGPDD95 pKa = 3.89FEE97 pKa = 5.11VSSYY101 pKa = 9.58WRR103 pKa = 11.84DD104 pKa = 3.19EE105 pKa = 3.95

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TR62|A0A4Y5TR62_9CAUD Uncharacterized protein OS=Klebsiella phage Marfa OX=2587809 GN=CPT_Marfa_232 PE=4 SV=1
MM1 pKa = 7.16QLEE4 pKa = 4.6NKK6 pKa = 9.27RR7 pKa = 11.84QVHH10 pKa = 5.15ATPRR14 pKa = 11.84RR15 pKa = 11.84WPIGSTPQGLYY26 pKa = 9.12IHH28 pKa = 7.09RR29 pKa = 11.84CISYY33 pKa = 9.48PRR35 pKa = 4.18

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

279

0

279

52116

30

1508

186.8

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.493 ± 0.179

1.13 ± 0.075

6.077 ± 0.101

7.159 ± 0.189

4.212 ± 0.125

6.297 ± 0.201

1.758 ± 0.094

6.867 ± 0.132

7.199 ± 0.18

7.506 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.08

5.175 ± 0.155

3.58 ± 0.106

3.438 ± 0.095

4.599 ± 0.099

6.426 ± 0.156

5.785 ± 0.228

6.858 ± 0.126

1.47 ± 0.066

4.166 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski