Actinomyces massiliensis F0489

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces massiliensis

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J1HN19|J1HN19_9ACTO Uncharacterized protein OS=Actinomyces massiliensis F0489 OX=1125718 GN=HMPREF1318_2069 PE=4 SV=1
MM1 pKa = 6.23SQKK4 pKa = 10.35RR5 pKa = 11.84FVSRR9 pKa = 11.84PRR11 pKa = 11.84AYY13 pKa = 9.93GVVGLVVVALCAWGLGSQHH32 pKa = 6.06GAAGGAKK39 pKa = 8.59DD40 pKa = 3.94TEE42 pKa = 4.58DD43 pKa = 3.73AEE45 pKa = 4.51HH46 pKa = 6.96AKK48 pKa = 10.35GAGYY52 pKa = 10.55FEE54 pKa = 4.99TVQSSGSSSVLLGDD68 pKa = 4.56DD69 pKa = 3.94SPVIDD74 pKa = 3.76TMRR77 pKa = 11.84SQDD80 pKa = 3.24VGAQEE85 pKa = 4.93LLVTGDD91 pKa = 3.7GVSTGSVSAVDD102 pKa = 3.45VDD104 pKa = 4.16GDD106 pKa = 4.32AEE108 pKa = 4.76LGTADD113 pKa = 3.37STTYY117 pKa = 10.87VYY119 pKa = 10.03TPPGYY124 pKa = 10.18DD125 pKa = 2.87AGSSTRR131 pKa = 11.84YY132 pKa = 8.6SVVYY136 pKa = 9.91LFHH139 pKa = 6.81GTPGWSSDD147 pKa = 3.27WFSGTALASRR157 pKa = 11.84LDD159 pKa = 3.63RR160 pKa = 11.84LIDD163 pKa = 4.35DD164 pKa = 4.39GAIPPVIAVSPEE176 pKa = 3.16ISTPEE181 pKa = 3.9VSDD184 pKa = 3.73TRR186 pKa = 11.84CLDD189 pKa = 3.4STTDD193 pKa = 3.36GPLIDD198 pKa = 4.19TYY200 pKa = 10.31LTQGVVPWVDD210 pKa = 3.45EE211 pKa = 4.64TYY213 pKa = 9.68STYY216 pKa = 11.41ADD218 pKa = 3.58ADD220 pKa = 3.57HH221 pKa = 6.62RR222 pKa = 11.84VLMGFSMGAFCATNLLFQHH241 pKa = 6.28QDD243 pKa = 3.15MFSSAAAMADD253 pKa = 3.48YY254 pKa = 10.95GEE256 pKa = 5.15PGADD260 pKa = 3.59AAPLLADD267 pKa = 3.56DD268 pKa = 4.84VEE270 pKa = 4.67YY271 pKa = 10.84EE272 pKa = 4.13RR273 pKa = 11.84QSPAVYY279 pKa = 10.48LSDD282 pKa = 3.93PDD284 pKa = 3.51FALRR288 pKa = 11.84DD289 pKa = 3.85GLSLYY294 pKa = 9.5LTVGGQSLDD303 pKa = 3.26VDD305 pKa = 3.87VDD307 pKa = 4.0DD308 pKa = 5.09TPLLADD314 pKa = 3.74MAAEE318 pKa = 4.01RR319 pKa = 11.84GVTVIYY325 pKa = 10.55EE326 pKa = 3.93PDD328 pKa = 3.06EE329 pKa = 4.57AADD332 pKa = 4.55HH333 pKa = 6.67DD334 pKa = 3.99WQMVSDD340 pKa = 4.27HH341 pKa = 6.86VDD343 pKa = 3.01GALEE347 pKa = 3.79MLLTDD352 pKa = 4.51GAA354 pKa = 4.26

Molecular weight: 37.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J1HLS1|J1HLS1_9ACTO Chaperone protein DnaK OS=Actinomyces massiliensis F0489 OX=1125718 GN=dnaK PE=2 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84HH12 pKa = 5.28RR13 pKa = 11.84AKK15 pKa = 10.38VHH17 pKa = 5.28GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.68GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3129

0

3129

984542

34

3202

314.7

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.608 ± 0.066

0.785 ± 0.013

6.133 ± 0.042

5.617 ± 0.04

2.469 ± 0.025

9.036 ± 0.041

2.025 ± 0.019

4.139 ± 0.032

1.865 ± 0.028

9.998 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.018

1.862 ± 0.024

5.671 ± 0.037

2.728 ± 0.026

7.585 ± 0.053

6.369 ± 0.035

6.191 ± 0.039

8.424 ± 0.038

1.485 ± 0.017

1.891 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski