Streptococcus phage Javan316

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B2I8|A0A4D6B2I8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan316 OX=2548104 GN=Javan316_0039 PE=4 SV=1
MM1 pKa = 7.91AILDD5 pKa = 4.57DD6 pKa = 4.75LQALYY11 pKa = 11.25DD12 pKa = 4.06NGWDD16 pKa = 3.14ASFVYY21 pKa = 10.04KK22 pKa = 10.89GQDD25 pKa = 3.32CAILPNSATDD35 pKa = 3.38IQVSIGAQTYY45 pKa = 8.5VVSSLDD51 pKa = 3.5DD52 pKa = 4.01LVNLDD57 pKa = 3.75IDD59 pKa = 4.11GQKK62 pKa = 10.62LSNIMSKK69 pKa = 9.13TEE71 pKa = 3.68VQYY74 pKa = 11.16YY75 pKa = 10.05

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AY47|A0A4D6AY47_9CAUD Uncharacterized protein OS=Streptococcus phage Javan316 OX=2548104 GN=Javan316_0046 PE=4 SV=1
MM1 pKa = 7.49GGASVKK7 pKa = 9.91IDD9 pKa = 3.47LKK11 pKa = 10.88GVEE14 pKa = 4.59KK15 pKa = 10.56KK16 pKa = 10.77VSPEE20 pKa = 3.45NFAKK24 pKa = 10.68GKK26 pKa = 9.91LAIANQMLLDD36 pKa = 3.67MDD38 pKa = 4.16RR39 pKa = 11.84FVPKK43 pKa = 10.24RR44 pKa = 11.84KK45 pKa = 10.15GILRR49 pKa = 11.84SSGHH53 pKa = 5.19VRR55 pKa = 11.84QDD57 pKa = 3.47SIIYY61 pKa = 8.08AAPHH65 pKa = 6.12ARR67 pKa = 11.84LLYY70 pKa = 9.83YY71 pKa = 10.24GKK73 pKa = 9.95KK74 pKa = 9.48RR75 pKa = 11.84KK76 pKa = 9.93GFFSEE81 pKa = 4.06KK82 pKa = 8.54QRR84 pKa = 11.84KK85 pKa = 8.15FFFANKK91 pKa = 9.44EE92 pKa = 3.94KK93 pKa = 10.8LLSQKK98 pKa = 7.49PTPGTGPRR106 pKa = 11.84WDD108 pKa = 3.86KK109 pKa = 10.91KK110 pKa = 11.03ASALYY115 pKa = 9.53AKK117 pKa = 10.06NWAEE121 pKa = 4.16VGAKK125 pKa = 10.44AMGVKK130 pKa = 10.02

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10994

42

1145

215.6

24.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.177 ± 0.773

0.528 ± 0.088

6.449 ± 0.307

7.904 ± 0.624

4.211 ± 0.215

6.886 ± 0.561

1.628 ± 0.216

6.813 ± 0.266

8.177 ± 0.519

8.459 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.203

5.085 ± 0.335

2.665 ± 0.21

4.12 ± 0.21

4.148 ± 0.311

6.558 ± 0.424

5.658 ± 0.241

6.203 ± 0.271

1.201 ± 0.151

3.62 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski