Mycoplasma agassizii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A269THY3|A0A269THY3_9MOLU Methionine adenosyltransferase OS=Mycoplasma agassizii OX=33922 GN=CJJ23_03955 PE=3 SV=1
MM1 pKa = 7.74ALLSILVVFEE11 pKa = 4.01YY12 pKa = 10.47TSAPFFVSEE21 pKa = 5.12LLLQATSEE29 pKa = 4.17ITTSEE34 pKa = 4.06LSSILVIVNNNHH46 pKa = 6.32LFLNFWVCEE55 pKa = 3.87RR56 pKa = 11.84NIWITFQYY64 pKa = 11.06LFGFEE69 pKa = 3.91II70 pKa = 4.47

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A269THZ9|A0A269THZ9_9MOLU Uncharacterized protein OS=Mycoplasma agassizii OX=33922 GN=CJJ23_04085 PE=4 SV=1
MM1 pKa = 7.39FKK3 pKa = 10.55NRR5 pKa = 11.84SARR8 pKa = 11.84VRR10 pKa = 11.84KK11 pKa = 9.02IRR13 pKa = 11.84KK14 pKa = 9.42RR15 pKa = 11.84NWLWKK20 pKa = 9.87QLVLFFKK27 pKa = 10.01EE28 pKa = 4.38LKK30 pKa = 9.37RR31 pKa = 11.84VRR33 pKa = 11.84WPKK36 pKa = 9.69TRR38 pKa = 11.84TSTHH42 pKa = 5.24QFLKK46 pKa = 10.55IIIFTIVFSLFVLAVATASSSVLNALGVGVV76 pKa = 4.1

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

955

0

955

356451

37

4582

373.2

42.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.014 ± 0.093

0.228 ± 0.011

5.628 ± 0.057

6.54 ± 0.095

5.401 ± 0.069

4.684 ± 0.072

1.43 ± 0.03

8.569 ± 0.09

9.53 ± 0.109

9.493 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.629 ± 0.036

7.43 ± 0.091

2.689 ± 0.048

3.297 ± 0.057

3.079 ± 0.053

7.296 ± 0.086

5.965 ± 0.114

6.017 ± 0.063

1.058 ± 0.024

4.025 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski