Nematocida displodere

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Nematocida

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177EAE3|A0A177EAE3_9MICR Uncharacterized protein OS=Nematocida displodere OX=1805483 GN=NEDG_01024 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84RR3 pKa = 11.84QAQHH7 pKa = 5.66RR8 pKa = 11.84CKK10 pKa = 10.59AILLLASLYY19 pKa = 9.31VLCGCIEE26 pKa = 4.18IDD28 pKa = 3.47PVVISTLEE36 pKa = 4.08DD37 pKa = 3.33FAKK40 pKa = 10.68DD41 pKa = 3.64LNEE44 pKa = 5.32KK45 pKa = 8.73PTIDD49 pKa = 3.09QNMFDD54 pKa = 4.47EE55 pKa = 5.26EE56 pKa = 4.27IKK58 pKa = 11.09ALTEE62 pKa = 3.92AKK64 pKa = 10.36LATEE68 pKa = 4.27EE69 pKa = 5.21KK70 pKa = 10.02IDD72 pKa = 3.87LLKK75 pKa = 10.77QRR77 pKa = 11.84GVQSGLATEE86 pKa = 4.6EE87 pKa = 4.48SNVPEE92 pKa = 4.19EE93 pKa = 3.92QSPAEE98 pKa = 4.03EE99 pKa = 3.76QLQAEE104 pKa = 4.57EE105 pKa = 4.06LAIGNDD111 pKa = 3.49EE112 pKa = 4.02QSEE115 pKa = 4.43VEE117 pKa = 4.17PEE119 pKa = 4.09VEE121 pKa = 4.05EE122 pKa = 4.36EE123 pKa = 3.97PTVFDD128 pKa = 3.64EE129 pKa = 4.01QSEE132 pKa = 4.55YY133 pKa = 11.06EE134 pKa = 4.09EE135 pKa = 4.41EE136 pKa = 4.07EE137 pKa = 4.19QYY139 pKa = 10.84EE140 pKa = 4.33AEE142 pKa = 4.52PATEE146 pKa = 3.74PTVFDD151 pKa = 3.66EE152 pKa = 4.29QLEE155 pKa = 4.35EE156 pKa = 4.22EE157 pKa = 4.87YY158 pKa = 10.69EE159 pKa = 4.32AEE161 pKa = 4.26PEE163 pKa = 4.06AADD166 pKa = 3.49EE167 pKa = 4.64AEE169 pKa = 4.11YY170 pKa = 10.9DD171 pKa = 3.88GQPEE175 pKa = 4.22EE176 pKa = 4.26QYY178 pKa = 10.53EE179 pKa = 4.12AQPEE183 pKa = 4.22PEE185 pKa = 4.25AEE187 pKa = 3.94EE188 pKa = 4.46EE189 pKa = 4.38YY190 pKa = 10.84EE191 pKa = 4.29PEE193 pKa = 4.48AEE195 pKa = 4.16PEE197 pKa = 3.97AEE199 pKa = 4.31EE200 pKa = 5.22EE201 pKa = 4.12YY202 pKa = 10.86DD203 pKa = 3.77GQPEE207 pKa = 4.09EE208 pKa = 4.21QYY210 pKa = 10.66EE211 pKa = 4.44AEE213 pKa = 4.46PEE215 pKa = 3.92AADD218 pKa = 4.44GIEE221 pKa = 3.79YY222 pKa = 10.43DD223 pKa = 3.92GQSEE227 pKa = 4.37EE228 pKa = 4.18QYY230 pKa = 10.2EE231 pKa = 4.24AQPEE235 pKa = 4.22AEE237 pKa = 5.05AEE239 pKa = 4.13AEE241 pKa = 4.21EE242 pKa = 4.15QSEE245 pKa = 4.39PEE247 pKa = 4.2EE248 pKa = 4.13QPEE251 pKa = 4.08AEE253 pKa = 4.17PEE255 pKa = 4.22VFDD258 pKa = 4.25EE259 pKa = 4.21QLEE262 pKa = 4.35AEE264 pKa = 4.66PEE266 pKa = 4.12PEE268 pKa = 4.17PEE270 pKa = 5.17AEE272 pKa = 4.37AEE274 pKa = 3.88YY275 pKa = 10.85DD276 pKa = 3.94GQSEE280 pKa = 4.61EE281 pKa = 3.98QDD283 pKa = 3.15EE284 pKa = 4.57AQPEE288 pKa = 4.44TQLEE292 pKa = 4.25AEE294 pKa = 4.43PEE296 pKa = 3.99AEE298 pKa = 4.22EE299 pKa = 5.15EE300 pKa = 4.01YY301 pKa = 10.86DD302 pKa = 3.79GQSEE306 pKa = 4.18EE307 pKa = 4.22QYY309 pKa = 10.84EE310 pKa = 4.38PEE312 pKa = 4.31AQPEE316 pKa = 4.17AEE318 pKa = 4.31PEE320 pKa = 3.96AEE322 pKa = 4.21AEE324 pKa = 4.48AEE326 pKa = 4.05AEE328 pKa = 3.84YY329 pKa = 10.87DD330 pKa = 3.83GQSEE334 pKa = 4.44EE335 pKa = 4.18QYY337 pKa = 9.98EE338 pKa = 4.34AQPEE342 pKa = 4.22TEE344 pKa = 3.76PAVFDD349 pKa = 3.96EE350 pKa = 4.26QLEE353 pKa = 4.34AEE355 pKa = 4.59PEE357 pKa = 4.11AEE359 pKa = 4.16PEE361 pKa = 4.08AEE363 pKa = 4.58PEE365 pKa = 4.49AEE367 pKa = 4.24TQLEE371 pKa = 4.24AEE373 pKa = 4.62PEE375 pKa = 4.06AEE377 pKa = 4.2AEE379 pKa = 4.48AEE381 pKa = 4.05AEE383 pKa = 3.84YY384 pKa = 10.87DD385 pKa = 3.83GQSEE389 pKa = 4.3EE390 pKa = 4.17QYY392 pKa = 10.24EE393 pKa = 4.25AEE395 pKa = 4.78PEE397 pKa = 4.14PEE399 pKa = 4.22TQLEE403 pKa = 4.34AEE405 pKa = 4.62PEE407 pKa = 4.09AEE409 pKa = 4.27PEE411 pKa = 4.2AEE413 pKa = 4.28ADD415 pKa = 3.37YY416 pKa = 11.23DD417 pKa = 3.97GQSEE421 pKa = 4.61YY422 pKa = 10.56EE423 pKa = 3.83AEE425 pKa = 4.0YY426 pKa = 10.9DD427 pKa = 4.57GEE429 pKa = 4.79SEE431 pKa = 4.26TQLEE435 pKa = 4.24EE436 pKa = 4.09EE437 pKa = 4.4EE438 pKa = 4.36QYY440 pKa = 10.85EE441 pKa = 4.37AEE443 pKa = 4.53PEE445 pKa = 3.96PTVYY449 pKa = 10.62DD450 pKa = 3.82EE451 pKa = 4.23QLEE454 pKa = 4.37AEE456 pKa = 4.58PEE458 pKa = 4.07AEE460 pKa = 3.97PEE462 pKa = 4.12AEE464 pKa = 4.21EE465 pKa = 5.15EE466 pKa = 4.01YY467 pKa = 10.86DD468 pKa = 3.79GQSEE472 pKa = 4.21EE473 pKa = 4.17QYY475 pKa = 10.29EE476 pKa = 4.3AEE478 pKa = 4.89AEE480 pKa = 4.22PEE482 pKa = 4.05AQPEE486 pKa = 4.29DD487 pKa = 3.51QTEE490 pKa = 4.36TQLEE494 pKa = 4.39AEE496 pKa = 4.83PEE498 pKa = 4.49TPAGPLPIAEE508 pKa = 4.98DD509 pKa = 3.84KK510 pKa = 11.46DD511 pKa = 4.37NIWVSTGPHH520 pKa = 5.01TEE522 pKa = 3.46EE523 pKa = 4.69DD524 pKa = 3.71VAHH527 pKa = 7.06RR528 pKa = 11.84EE529 pKa = 4.16SAEE532 pKa = 4.11CTKK535 pKa = 11.02DD536 pKa = 3.37DD537 pKa = 3.75EE538 pKa = 4.52KK539 pKa = 11.62VYY541 pKa = 10.88GFIMKK546 pKa = 10.06HH547 pKa = 5.22NPSLLGDD554 pKa = 3.87AEE556 pKa = 4.36QPFRR560 pKa = 11.84CDD562 pKa = 4.68LYY564 pKa = 9.41NQCRR568 pKa = 11.84NTITHH573 pKa = 5.73VSIDD577 pKa = 3.35LSIYY581 pKa = 10.55EE582 pKa = 4.8KK583 pKa = 11.07GSLPNQEE590 pKa = 3.4VWKK593 pKa = 10.62SFKK596 pKa = 10.45NLQGFEE602 pKa = 3.76IAGNKK607 pKa = 7.1NTEE610 pKa = 4.01ALPNLSFALEE620 pKa = 4.17DD621 pKa = 3.37VSAAVSDD628 pKa = 4.4QEE630 pKa = 4.15DD631 pKa = 3.4QKK633 pKa = 11.46LSKK636 pKa = 10.57LVLSGVDD643 pKa = 3.67LSEE646 pKa = 4.1QTEE649 pKa = 4.07ALQNLKK655 pKa = 10.15NVRR658 pKa = 11.84KK659 pKa = 9.86LYY661 pKa = 10.95LFDD664 pKa = 3.5STFNASEE671 pKa = 4.1GAPNLLLTLFQCPGLSEE688 pKa = 4.6LKK690 pKa = 10.46VISSSISAIPSAGEE704 pKa = 3.74PASSCLSLHH713 pKa = 6.77ILRR716 pKa = 11.84CSQEE720 pKa = 4.07TVRR723 pKa = 11.84ALACTPASTFATVILNEE740 pKa = 4.08NTLGDD745 pKa = 4.38LEE747 pKa = 4.67CLNDD751 pKa = 3.96AEE753 pKa = 4.79SSIASLVIQSDD764 pKa = 3.61QTIKK768 pKa = 10.69SVKK771 pKa = 10.26KK772 pKa = 10.34SDD774 pKa = 3.82LQALPALKK782 pKa = 10.24SLCLFDD788 pKa = 5.16LQPSYY793 pKa = 11.05EE794 pKa = 4.39IDD796 pKa = 3.77PEE798 pKa = 4.06ILGPDD803 pKa = 3.55MPVSEE808 pKa = 4.81IFFSEE813 pKa = 3.9NEE815 pKa = 3.92EE816 pKa = 4.46FNFSSALPKK825 pKa = 10.26EE826 pKa = 4.17YY827 pKa = 10.67LKK829 pKa = 11.05NKK831 pKa = 9.14HH832 pKa = 6.2LGVYY836 pKa = 7.15THH838 pKa = 6.61GKK840 pKa = 9.0IEE842 pKa = 3.96NEE844 pKa = 4.28RR845 pKa = 11.84IFHH848 pKa = 6.4LVVDD852 pKa = 4.97PDD854 pKa = 3.69AKK856 pKa = 10.13TALLVIDD863 pKa = 4.92SPNDD867 pKa = 3.28FANTSEE873 pKa = 4.34AFKK876 pKa = 11.34KK877 pKa = 9.83MVEE880 pKa = 4.44SVNEE884 pKa = 3.87LDD886 pKa = 4.05TINVVVNTTDD896 pKa = 4.16DD897 pKa = 3.78SQKK900 pKa = 10.72EE901 pKa = 4.07AVTQKK906 pKa = 9.88ILSIFSHH913 pKa = 7.0LKK915 pKa = 7.0TQPTVDD921 pKa = 3.74YY922 pKa = 9.94TPKK925 pKa = 10.67NN926 pKa = 3.48

Molecular weight: 103.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177EJP7|A0A177EJP7_9MICR Serine/threonine-protein kinase MRCK OS=Nematocida displodere OX=1805483 GN=NEDG_01622 PE=4 SV=1
MM1 pKa = 7.08IWGIEE6 pKa = 3.96RR7 pKa = 11.84LSVHH11 pKa = 5.81RR12 pKa = 11.84QKK14 pKa = 11.16EE15 pKa = 4.23WTHH18 pKa = 5.03TLAAPFLKK26 pKa = 9.65LTASLTRR33 pKa = 11.84THH35 pKa = 6.56VIISATYY42 pKa = 8.23FGSEE46 pKa = 3.97EE47 pKa = 4.06VLVCVLAAAATSVHH61 pKa = 6.22CSKK64 pKa = 11.05DD65 pKa = 3.22LALFAGPLRR74 pKa = 11.84VQLKK78 pKa = 9.7LCSARR83 pKa = 11.84AAQTLHH89 pKa = 6.51RR90 pKa = 11.84RR91 pKa = 11.84LIYY94 pKa = 6.91TQKK97 pKa = 9.5MLRR100 pKa = 11.84FIQGMQKK107 pKa = 9.76VRR109 pKa = 11.84LPSASAPGPPRR120 pKa = 4.47

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2241

0

2241

862370

52

3243

384.8

43.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.047

1.76 ± 0.024

4.327 ± 0.03

7.322 ± 0.062

4.13 ± 0.036

5.61 ± 0.044

2.278 ± 0.02

6.396 ± 0.036

6.806 ± 0.048

10.434 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.019

3.686 ± 0.037

4.688 ± 0.053

3.469 ± 0.027

5.04 ± 0.034

7.65 ± 0.047

6.499 ± 0.041

6.659 ± 0.04

0.869 ± 0.014

3.096 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski