Amino acid dipepetide frequency for Staphylococcus phage Stab22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.07AlaAla: 0.07 ± 0.043
0.21AlaCys: 0.21 ± 0.067
2.334AlaAsp: 2.334 ± 0.257
3.127AlaGlu: 3.127 ± 0.382
1.727AlaPhe: 1.727 ± 0.173
2.45AlaGly: 2.45 ± 0.357
0.723AlaHis: 0.723 ± 0.13
3.571AlaIle: 3.571 ± 0.311
4.177AlaLys: 4.177 ± 0.402
3.43AlaLeu: 3.43 ± 0.333
1.214AlaMet: 1.214 ± 0.222
2.474AlaAsn: 2.474 ± 0.255
1.167AlaPro: 1.167 ± 0.207
1.844AlaGln: 1.844 ± 0.288
1.54AlaArg: 1.54 ± 0.177
3.384AlaSer: 3.384 ± 0.364
3.244AlaThr: 3.244 ± 0.303
3.034AlaVal: 3.034 ± 0.266
0.443AlaTrp: 0.443 ± 0.116
2.147AlaTyr: 2.147 ± 0.238
0.0AlaXaa: 0.0 ± 0.0
Cys
0.21CysAla: 0.21 ± 0.064
0.07CysCys: 0.07 ± 0.041
0.397CysAsp: 0.397 ± 0.108
0.257CysGlu: 0.257 ± 0.085
0.21CysPhe: 0.21 ± 0.075
0.327CysGly: 0.327 ± 0.088
0.093CysHis: 0.093 ± 0.047
0.373CysIle: 0.373 ± 0.093
0.583CysLys: 0.583 ± 0.153
0.49CysLeu: 0.49 ± 0.12
0.093CysMet: 0.093 ± 0.051
0.047CysAsn: 0.047 ± 0.033
0.257CysPro: 0.257 ± 0.067
0.21CysGln: 0.21 ± 0.083
0.257CysArg: 0.257 ± 0.092
0.35CysSer: 0.35 ± 0.081
0.303CysThr: 0.303 ± 0.074
0.373CysVal: 0.373 ± 0.088
0.047CysTrp: 0.047 ± 0.032
0.233CysTyr: 0.233 ± 0.072
0.0CysXaa: 0.0 ± 0.0
Asp
2.917AspAla: 2.917 ± 0.365
0.397AspCys: 0.397 ± 0.116
4.084AspAsp: 4.084 ± 0.389
5.694AspGlu: 5.694 ± 0.423
3.547AspPhe: 3.547 ± 0.34
3.36AspGly: 3.36 ± 0.288
0.513AspHis: 0.513 ± 0.115
6.581AspIle: 6.581 ± 0.432
6.231AspLys: 6.231 ± 0.375
5.974AspLeu: 5.974 ± 0.383
2.1AspMet: 2.1 ± 0.217
6.184AspAsn: 6.184 ± 0.5
1.587AspPro: 1.587 ± 0.186
1.027AspGln: 1.027 ± 0.192
2.45AspArg: 2.45 ± 0.274
4.574AspSer: 4.574 ± 0.374
4.224AspThr: 4.224 ± 0.315
4.737AspVal: 4.737 ± 0.374
0.537AspTrp: 0.537 ± 0.105
4.177AspTyr: 4.177 ± 0.391
0.0AspXaa: 0.0 ± 0.0
Glu
4.084GluAla: 4.084 ± 0.34
0.397GluCys: 0.397 ± 0.124
7.001GluAsp: 7.001 ± 0.51
9.801GluGlu: 9.801 ± 0.842
3.267GluPhe: 3.267 ± 0.286
4.784GluGly: 4.784 ± 0.29
1.704GluHis: 1.704 ± 0.235
5.344GluIle: 5.344 ± 0.427
7.118GluLys: 7.118 ± 0.47
8.028GluLeu: 8.028 ± 0.433
2.334GluMet: 2.334 ± 0.249
4.341GluAsn: 4.341 ± 0.316
2.007GluPro: 2.007 ± 0.399
4.014GluGln: 4.014 ± 0.35
3.174GluArg: 3.174 ± 0.277
4.691GluSer: 4.691 ± 0.316
3.804GluThr: 3.804 ± 0.295
5.601GluVal: 5.601 ± 0.455
0.7GluTrp: 0.7 ± 0.124
4.037GluTyr: 4.037 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
1.517PheAla: 1.517 ± 0.164
0.233PheCys: 0.233 ± 0.077
2.987PheAsp: 2.987 ± 0.268
2.707PheGlu: 2.707 ± 0.239
1.447PhePhe: 1.447 ± 0.153
1.937PheGly: 1.937 ± 0.241
0.747PheHis: 0.747 ± 0.133
3.501PheIle: 3.501 ± 0.307
3.874PheLys: 3.874 ± 0.319
3.314PheLeu: 3.314 ± 0.311
0.98PheMet: 0.98 ± 0.172
3.384PheAsn: 3.384 ± 0.265
0.98PhePro: 0.98 ± 0.129
1.237PheGln: 1.237 ± 0.156
1.167PheArg: 1.167 ± 0.16
2.94PheSer: 2.94 ± 0.288
2.497PheThr: 2.497 ± 0.247
2.357PheVal: 2.357 ± 0.276
0.397PheTrp: 0.397 ± 0.106
2.427PheTyr: 2.427 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
2.45GlyAla: 2.45 ± 0.455
0.303GlyCys: 0.303 ± 0.076
4.014GlyAsp: 4.014 ± 0.38
4.364GlyGlu: 4.364 ± 0.355
2.24GlyPhe: 2.24 ± 0.258
3.991GlyGly: 3.991 ± 0.816
1.05GlyHis: 1.05 ± 0.161
4.224GlyIle: 4.224 ± 0.314
5.577GlyLys: 5.577 ± 0.412
4.994GlyLeu: 4.994 ± 0.355
1.237GlyMet: 1.237 ± 0.184
3.664GlyAsn: 3.664 ± 0.278
0.0GlyPro: 0.0 ± 0.0
1.704GlyGln: 1.704 ± 0.255
2.147GlyArg: 2.147 ± 0.263
4.644GlySer: 4.644 ± 0.507
3.804GlyThr: 3.804 ± 0.446
3.827GlyVal: 3.827 ± 0.307
0.467GlyTrp: 0.467 ± 0.138
3.501GlyTyr: 3.501 ± 0.292
0.023GlyXaa: 0.023 ± 0.023
His
0.607HisAla: 0.607 ± 0.108
0.233HisCys: 0.233 ± 0.068
1.05HisAsp: 1.05 ± 0.157
1.143HisGlu: 1.143 ± 0.183
0.747HisPhe: 0.747 ± 0.132
1.073HisGly: 1.073 ± 0.134
0.187HisHis: 0.187 ± 0.069
1.47HisIle: 1.47 ± 0.228
1.167HisLys: 1.167 ± 0.152
1.494HisLeu: 1.494 ± 0.184
0.303HisMet: 0.303 ± 0.094
1.027HisAsn: 1.027 ± 0.145
0.537HisPro: 0.537 ± 0.113
0.537HisGln: 0.537 ± 0.11
0.677HisArg: 0.677 ± 0.134
0.747HisSer: 0.747 ± 0.144
1.097HisThr: 1.097 ± 0.176
1.073HisVal: 1.073 ± 0.151
0.257HisTrp: 0.257 ± 0.105
0.677HisTyr: 0.677 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
2.987IleAla: 2.987 ± 0.276
0.443IleCys: 0.443 ± 0.13
5.741IleAsp: 5.741 ± 0.371
6.604IleGlu: 6.604 ± 0.426
2.31IlePhe: 2.31 ± 0.245
3.827IleGly: 3.827 ± 0.374
1.167IleHis: 1.167 ± 0.159
6.044IleIle: 6.044 ± 0.448
7.094IleLys: 7.094 ± 0.431
5.344IleLeu: 5.344 ± 0.415
1.797IleMet: 1.797 ± 0.232
5.484IleAsn: 5.484 ± 0.354
2.124IlePro: 2.124 ± 0.189
2.38IleGln: 2.38 ± 0.237
2.684IleArg: 2.684 ± 0.246
4.737IleSer: 4.737 ± 0.314
5.251IleThr: 5.251 ± 0.368
4.504IleVal: 4.504 ± 0.325
0.467IleTrp: 0.467 ± 0.104
2.754IleTyr: 2.754 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
3.874LysAla: 3.874 ± 0.403
0.327LysCys: 0.327 ± 0.114
7.048LysAsp: 7.048 ± 0.439
10.291LysGlu: 10.291 ± 0.675
2.917LysPhe: 2.917 ± 0.239
5.974LysGly: 5.974 ± 0.513
1.634LysHis: 1.634 ± 0.191
4.341LysIle: 4.341 ± 0.263
7.024LysLys: 7.024 ± 0.541
6.581LysLeu: 6.581 ± 0.349
2.147LysMet: 2.147 ± 0.273
5.041LysAsn: 5.041 ± 0.354
2.544LysPro: 2.544 ± 0.271
3.594LysGln: 3.594 ± 0.325
2.94LysArg: 2.94 ± 0.313
4.737LysSer: 4.737 ± 0.325
4.691LysThr: 4.691 ± 0.38
6.768LysVal: 6.768 ± 0.313
0.653LysTrp: 0.653 ± 0.101
4.387LysTyr: 4.387 ± 0.364
0.0LysXaa: 0.0 ± 0.0
Leu
3.921LeuAla: 3.921 ± 0.358
0.49LeuCys: 0.49 ± 0.117
6.021LeuAsp: 6.021 ± 0.353
7.631LeuGlu: 7.631 ± 0.576
3.36LeuPhe: 3.36 ± 0.253
4.737LeuGly: 4.737 ± 0.466
0.933LeuHis: 0.933 ± 0.156
5.858LeuIle: 5.858 ± 0.386
6.791LeuLys: 6.791 ± 0.402
6.184LeuLeu: 6.184 ± 0.427
2.1LeuMet: 2.1 ± 0.231
5.391LeuAsn: 5.391 ± 0.349
2.707LeuPro: 2.707 ± 0.256
3.22LeuGln: 3.22 ± 0.307
3.15LeuArg: 3.15 ± 0.263
5.437LeuSer: 5.437 ± 0.423
5.344LeuThr: 5.344 ± 0.387
4.551LeuVal: 4.551 ± 0.349
0.607LeuTrp: 0.607 ± 0.108
2.987LeuTyr: 2.987 ± 0.315
0.0LeuXaa: 0.0 ± 0.0
Met
1.564MetAla: 1.564 ± 0.187
0.21MetCys: 0.21 ± 0.085
1.774MetAsp: 1.774 ± 0.196
2.007MetGlu: 2.007 ± 0.271
1.073MetPhe: 1.073 ± 0.156
0.957MetGly: 0.957 ± 0.19
0.35MetHis: 0.35 ± 0.094
1.517MetIle: 1.517 ± 0.195
2.287MetLys: 2.287 ± 0.244
1.774MetLeu: 1.774 ± 0.202
0.723MetMet: 0.723 ± 0.169
1.774MetAsn: 1.774 ± 0.229
0.56MetPro: 0.56 ± 0.135
0.817MetGln: 0.817 ± 0.136
1.377MetArg: 1.377 ± 0.19
1.984MetSer: 1.984 ± 0.247
1.657MetThr: 1.657 ± 0.172
1.167MetVal: 1.167 ± 0.172
0.14MetTrp: 0.14 ± 0.053
0.933MetTyr: 0.933 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
2.894AsnAla: 2.894 ± 0.268
0.257AsnCys: 0.257 ± 0.079
3.874AsnAsp: 3.874 ± 0.321
4.667AsnGlu: 4.667 ± 0.382
2.544AsnPhe: 2.544 ± 0.269
4.014AsnGly: 4.014 ± 0.279
1.12AsnHis: 1.12 ± 0.181
5.064AsnIle: 5.064 ± 0.391
6.651AsnLys: 6.651 ± 0.417
5.694AsnLeu: 5.694 ± 0.415
1.564AsnMet: 1.564 ± 0.172
5.227AsnAsn: 5.227 ± 0.444
2.66AsnPro: 2.66 ± 0.304
2.287AsnGln: 2.287 ± 0.223
2.45AsnArg: 2.45 ± 0.239
3.967AsnSer: 3.967 ± 0.291
3.711AsnThr: 3.711 ± 0.259
3.664AsnVal: 3.664 ± 0.304
0.607AsnTrp: 0.607 ± 0.119
3.104AsnTyr: 3.104 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
1.097ProAla: 1.097 ± 0.186
0.07ProCys: 0.07 ± 0.037
1.54ProAsp: 1.54 ± 0.223
2.427ProGlu: 2.427 ± 0.307
1.073ProPhe: 1.073 ± 0.15
1.027ProGly: 1.027 ± 0.174
0.467ProHis: 0.467 ± 0.102
1.914ProIle: 1.914 ± 0.231
2.474ProLys: 2.474 ± 0.258
1.984ProLeu: 1.984 ± 0.19
0.63ProMet: 0.63 ± 0.12
2.077ProAsn: 2.077 ± 0.222
0.817ProPro: 0.817 ± 0.289
1.237ProGln: 1.237 ± 0.212
0.817ProArg: 0.817 ± 0.146
2.217ProSer: 2.217 ± 0.287
1.914ProThr: 1.914 ± 0.276
1.727ProVal: 1.727 ± 0.223
0.163ProTrp: 0.163 ± 0.06
1.68ProTyr: 1.68 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
2.17GlnAla: 2.17 ± 0.226
0.093GlnCys: 0.093 ± 0.048
2.87GlnAsp: 2.87 ± 0.279
3.477GlnGlu: 3.477 ± 0.348
1.634GlnPhe: 1.634 ± 0.188
2.567GlnGly: 2.567 ± 0.28
0.677GlnHis: 0.677 ± 0.128
2.1GlnIle: 2.1 ± 0.27
2.147GlnLys: 2.147 ± 0.215
3.314GlnLeu: 3.314 ± 0.316
0.817GlnMet: 0.817 ± 0.136
1.564GlnAsn: 1.564 ± 0.194
1.003GlnPro: 1.003 ± 0.192
1.984GlnGln: 1.984 ± 0.404
1.167GlnArg: 1.167 ± 0.156
2.544GlnSer: 2.544 ± 0.227
1.75GlnThr: 1.75 ± 0.21
2.03GlnVal: 2.03 ± 0.283
0.327GlnTrp: 0.327 ± 0.1
1.89GlnTyr: 1.89 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
1.447ArgAla: 1.447 ± 0.185
0.187ArgCys: 0.187 ± 0.072
2.987ArgAsp: 2.987 ± 0.236
2.544ArgGlu: 2.544 ± 0.241
1.82ArgPhe: 1.82 ± 0.184
2.194ArgGly: 2.194 ± 0.202
0.467ArgHis: 0.467 ± 0.09
2.66ArgIle: 2.66 ± 0.258
3.36ArgLys: 3.36 ± 0.342
2.87ArgLeu: 2.87 ± 0.258
0.91ArgMet: 0.91 ± 0.121
2.03ArgAsn: 2.03 ± 0.226
1.167ArgPro: 1.167 ± 0.165
1.447ArgGln: 1.447 ± 0.173
1.54ArgArg: 1.54 ± 0.247
1.61ArgSer: 1.61 ± 0.218
2.054ArgThr: 2.054 ± 0.272
2.59ArgVal: 2.59 ± 0.251
0.327ArgTrp: 0.327 ± 0.086
1.634ArgTyr: 1.634 ± 0.214
0.0ArgXaa: 0.0 ± 0.0
Ser
2.847SerAla: 2.847 ± 0.289
0.187SerCys: 0.187 ± 0.068
4.107SerAsp: 4.107 ± 0.303
4.247SerGlu: 4.247 ± 0.29
3.104SerPhe: 3.104 ± 0.298
3.921SerGly: 3.921 ± 0.415
1.307SerHis: 1.307 ± 0.175
5.041SerIle: 5.041 ± 0.384
5.717SerLys: 5.717 ± 0.434
5.321SerLeu: 5.321 ± 0.353
1.657SerMet: 1.657 ± 0.151
4.574SerAsn: 4.574 ± 0.319
1.96SerPro: 1.96 ± 0.199
2.17SerGln: 2.17 ± 0.261
1.937SerArg: 1.937 ± 0.202
4.714SerSer: 4.714 ± 0.481
3.944SerThr: 3.944 ± 0.319
3.991SerVal: 3.991 ± 0.283
0.677SerTrp: 0.677 ± 0.135
3.781SerTyr: 3.781 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
2.45ThrAla: 2.45 ± 0.217
0.163ThrCys: 0.163 ± 0.058
4.271ThrAsp: 4.271 ± 0.361
5.321ThrGlu: 5.321 ± 0.386
2.684ThrPhe: 2.684 ± 0.277
4.107ThrGly: 4.107 ± 0.328
1.003ThrHis: 1.003 ± 0.183
5.134ThrIle: 5.134 ± 0.435
4.807ThrLys: 4.807 ± 0.331
4.597ThrLeu: 4.597 ± 0.308
1.05ThrMet: 1.05 ± 0.14
3.43ThrAsn: 3.43 ± 0.267
2.287ThrPro: 2.287 ± 0.283
2.287ThrGln: 2.287 ± 0.274
2.287ThrArg: 2.287 ± 0.253
4.061ThrSer: 4.061 ± 0.345
3.337ThrThr: 3.337 ± 0.39
4.294ThrVal: 4.294 ± 0.332
0.537ThrTrp: 0.537 ± 0.117
2.497ThrTyr: 2.497 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
2.59ValAla: 2.59 ± 0.268
0.537ValCys: 0.537 ± 0.114
4.294ValAsp: 4.294 ± 0.264
5.741ValGlu: 5.741 ± 0.485
2.777ValPhe: 2.777 ± 0.312
3.501ValGly: 3.501 ± 0.255
1.073ValHis: 1.073 ± 0.163
4.877ValIle: 4.877 ± 0.344
5.647ValLys: 5.647 ± 0.419
5.297ValLeu: 5.297 ± 0.347
1.284ValMet: 1.284 ± 0.19
4.084ValAsn: 4.084 ± 0.324
1.68ValPro: 1.68 ± 0.196
1.914ValGln: 1.914 ± 0.183
2.17ValArg: 2.17 ± 0.214
4.597ValSer: 4.597 ± 0.358
4.294ValThr: 4.294 ± 0.338
3.827ValVal: 3.827 ± 0.356
0.443ValTrp: 0.443 ± 0.099
3.104ValTyr: 3.104 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
0.513TrpAla: 0.513 ± 0.11
0.047TrpCys: 0.047 ± 0.028
0.583TrpAsp: 0.583 ± 0.115
0.887TrpGlu: 0.887 ± 0.126
0.35TrpPhe: 0.35 ± 0.109
0.653TrpGly: 0.653 ± 0.138
0.093TrpHis: 0.093 ± 0.052
0.607TrpIle: 0.607 ± 0.113
0.677TrpLys: 0.677 ± 0.125
0.42TrpLeu: 0.42 ± 0.11
0.14TrpMet: 0.14 ± 0.054
0.677TrpAsn: 0.677 ± 0.115
0.0TrpPro: 0.0 ± 0.0
0.35TrpGln: 0.35 ± 0.104
0.163TrpArg: 0.163 ± 0.046
0.467TrpSer: 0.467 ± 0.11
0.443TrpThr: 0.443 ± 0.095
0.467TrpVal: 0.467 ± 0.09
0.163TrpTrp: 0.163 ± 0.072
0.653TrpTyr: 0.653 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.1TyrAla: 2.1 ± 0.245
0.327TyrCys: 0.327 ± 0.078
3.921TyrAsp: 3.921 ± 0.302
3.29TyrGlu: 3.29 ± 0.285
1.797TyrPhe: 1.797 ± 0.227
2.824TyrGly: 2.824 ± 0.277
0.793TyrHis: 0.793 ± 0.132
3.524TyrIle: 3.524 ± 0.325
4.084TyrLys: 4.084 ± 0.317
4.317TyrLeu: 4.317 ± 0.321
1.54TyrMet: 1.54 ± 0.204
3.547TyrAsn: 3.547 ± 0.32
1.307TyrPro: 1.307 ± 0.186
1.867TyrGln: 1.867 ± 0.208
1.75TyrArg: 1.75 ± 0.188
2.8TyrSer: 2.8 ± 0.255
3.244TyrThr: 3.244 ± 0.309
3.174TyrVal: 3.174 ± 0.297
0.397TyrTrp: 0.397 ± 0.107
3.244TyrTyr: 3.244 ± 0.319
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.023XaaLys: 0.023 ± 0.023
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 211 proteins (42852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski