Streptococcus phage IC1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060QR77|A0A060QR77_9CAUD Putative phage Holliday junction resolvase OS=Streptococcus phage IC1 OX=1448276 PE=4 SV=1
MM1 pKa = 7.28FFSDD5 pKa = 5.64DD6 pKa = 3.53ILAIDD11 pKa = 4.18YY12 pKa = 10.11EE13 pKa = 4.32NEE15 pKa = 4.15EE16 pKa = 4.33IVTQQIYY23 pKa = 10.29FEE25 pKa = 4.9NGLPDD30 pKa = 4.88DD31 pKa = 4.65RR32 pKa = 11.84DD33 pKa = 3.7IYY35 pKa = 10.92CYY37 pKa = 10.86NPDD40 pKa = 4.59EE41 pKa = 5.02IEE43 pKa = 4.3LMQSTGLKK51 pKa = 10.09DD52 pKa = 3.55KK53 pKa = 10.88NGKK56 pKa = 8.29EE57 pKa = 4.02VFIGDD62 pKa = 3.33IVKK65 pKa = 8.82CTRR68 pKa = 11.84GCLHH72 pKa = 6.5EE73 pKa = 4.82VYY75 pKa = 10.55LEE77 pKa = 4.0KK78 pKa = 10.57EE79 pKa = 4.02YY80 pKa = 11.28GGTFIGGMPAVYY92 pKa = 10.19LKK94 pKa = 11.23GFGDD98 pKa = 4.85GYY100 pKa = 11.33AWTEE104 pKa = 3.77YY105 pKa = 11.1EE106 pKa = 4.26EE107 pKa = 4.98IIGNIYY113 pKa = 9.86EE114 pKa = 4.19NPEE117 pKa = 4.11LLEE120 pKa = 4.3VTHH123 pKa = 6.38EE124 pKa = 4.03

Molecular weight: 14.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060QNC5|A0A060QNC5_9CAUD Phage protein OS=Streptococcus phage IC1 OX=1448276 PE=4 SV=1
MM1 pKa = 6.96QRR3 pKa = 11.84RR4 pKa = 11.84EE5 pKa = 3.9IMNDD9 pKa = 3.93FIKK12 pKa = 10.46EE13 pKa = 3.43IGMAILWMFLGYY25 pKa = 10.88LLGEE29 pKa = 4.21RR30 pKa = 11.84STRR33 pKa = 11.84GGQIRR38 pKa = 3.63

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10978

38

2417

224.0

25.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.16 ± 0.625

0.656 ± 0.118

6.349 ± 0.358

8.253 ± 0.621

3.999 ± 0.285

6.723 ± 0.606

1.175 ± 0.149

6.34 ± 0.326

8.062 ± 0.351

7.998 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.27

5.347 ± 0.292

2.578 ± 0.222

4.327 ± 0.3

4.655 ± 0.389

6.313 ± 0.469

6.112 ± 0.467

6.531 ± 0.269

1.439 ± 0.208

3.689 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski