Caulobacter mirabilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Caulobacter

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2AXM9|A0A2D2AXM9_9CAUL AraC family transcriptional regulator OS=Caulobacter mirabilis OX=69666 GN=CSW64_10070 PE=4 SV=1
MM1 pKa = 7.16PQATVSMIYY10 pKa = 10.05CVQQATGTDD19 pKa = 3.08AGVNEE24 pKa = 5.55AIAGLPEE31 pKa = 4.35LLLPGEE37 pKa = 4.33FGGMPLQAIRR47 pKa = 11.84ALPGVIAAVDD57 pKa = 3.53AARR60 pKa = 11.84ADD62 pKa = 3.88PDD64 pKa = 3.75DD65 pKa = 5.74LYY67 pKa = 10.37ITTSTGGGRR76 pKa = 11.84DD77 pKa = 3.36NAIWPSAGGVVQIQAGQSEE96 pKa = 4.83TPDD99 pKa = 3.04VTVAFDD105 pKa = 3.49HH106 pKa = 6.24SQNLSLWDD114 pKa = 3.84YY115 pKa = 11.65DD116 pKa = 4.29SVSDD120 pKa = 5.17DD121 pKa = 4.36DD122 pKa = 5.33LLGSVTMFASEE133 pKa = 3.93QGQGEE138 pKa = 4.19IAKK141 pKa = 9.56LAKK144 pKa = 10.46SPIEE148 pKa = 3.56NSYY151 pKa = 9.75YY152 pKa = 9.01YY153 pKa = 10.53VVYY156 pKa = 10.41RR157 pKa = 11.84VDD159 pKa = 3.01

Molecular weight: 16.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2B3N3|A0A2D2B3N3_9CAUL Protein tyrosine phosphatase OS=Caulobacter mirabilis OX=69666 GN=CSW64_04120 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSRR10 pKa = 11.84LVRR13 pKa = 11.84KK14 pKa = 9.11RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.53GFRR20 pKa = 11.84LRR22 pKa = 11.84MSTKK26 pKa = 10.23NGAKK30 pKa = 9.76IIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.93GRR40 pKa = 11.84KK41 pKa = 8.85RR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4239

0

4239

1376727

28

4922

324.8

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.931 ± 0.061

0.699 ± 0.012

5.857 ± 0.03

5.469 ± 0.047

3.529 ± 0.024

9.33 ± 0.09

1.673 ± 0.021

4.251 ± 0.028

3.024 ± 0.035

9.998 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.023

2.321 ± 0.031

5.563 ± 0.04

3.008 ± 0.022

7.447 ± 0.052

4.972 ± 0.04

5.403 ± 0.067

7.637 ± 0.033

1.522 ± 0.018

2.211 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski