Amino acid dipepetide frequency for Streptococcus phage Javan180

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.179AlaAla: 6.179 ± 1.57
0.524AlaCys: 0.524 ± 0.196
4.713AlaAsp: 4.713 ± 0.53
5.655AlaGlu: 5.655 ± 0.98
3.142AlaPhe: 3.142 ± 0.824
5.131AlaGly: 5.131 ± 1.299
0.524AlaHis: 0.524 ± 0.196
6.388AlaIle: 6.388 ± 0.834
8.064AlaLys: 8.064 ± 1.058
5.76AlaLeu: 5.76 ± 0.793
2.723AlaMet: 2.723 ± 0.677
3.246AlaAsn: 3.246 ± 0.572
1.047AlaPro: 1.047 ± 0.308
2.618AlaGln: 2.618 ± 0.49
2.828AlaArg: 2.828 ± 0.609
4.713AlaSer: 4.713 ± 0.643
5.131AlaThr: 5.131 ± 1.088
4.398AlaVal: 4.398 ± 1.416
0.733AlaTrp: 0.733 ± 0.241
1.676AlaTyr: 1.676 ± 0.338
0.0AlaXaa: 0.0 ± 0.0
Cys
0.314CysAla: 0.314 ± 0.159
0.0CysCys: 0.0 ± 0.0
0.419CysAsp: 0.419 ± 0.248
0.419CysGlu: 0.419 ± 0.241
0.105CysPhe: 0.105 ± 0.095
0.419CysGly: 0.419 ± 0.186
0.105CysHis: 0.105 ± 0.1
0.209CysIle: 0.209 ± 0.122
0.314CysLys: 0.314 ± 0.176
0.838CysLeu: 0.838 ± 0.244
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.105CysGln: 0.105 ± 0.085
0.419CysArg: 0.419 ± 0.203
0.105CysSer: 0.105 ± 0.095
0.209CysThr: 0.209 ± 0.148
0.524CysVal: 0.524 ± 0.237
0.105CysTrp: 0.105 ± 0.097
0.314CysTyr: 0.314 ± 0.194
0.0CysXaa: 0.0 ± 0.0
Asp
3.142AspAla: 3.142 ± 0.731
0.209AspCys: 0.209 ± 0.156
3.979AspAsp: 3.979 ± 0.629
4.294AspGlu: 4.294 ± 0.862
2.513AspPhe: 2.513 ± 0.395
4.922AspGly: 4.922 ± 0.606
0.838AspHis: 0.838 ± 0.323
4.922AspIle: 4.922 ± 0.738
6.912AspLys: 6.912 ± 0.831
6.702AspLeu: 6.702 ± 1.024
1.257AspMet: 1.257 ± 0.437
3.351AspAsn: 3.351 ± 0.694
1.99AspPro: 1.99 ± 0.357
1.571AspGln: 1.571 ± 0.35
3.037AspArg: 3.037 ± 0.642
5.446AspSer: 5.446 ± 0.738
3.246AspThr: 3.246 ± 0.554
4.817AspVal: 4.817 ± 0.687
0.524AspTrp: 0.524 ± 0.316
4.608AspTyr: 4.608 ± 0.782
0.0AspXaa: 0.0 ± 0.0
Glu
7.121GluAla: 7.121 ± 1.279
0.0GluCys: 0.0 ± 0.0
2.828GluAsp: 2.828 ± 0.64
5.341GluGlu: 5.341 ± 1.038
3.037GluPhe: 3.037 ± 0.469
3.979GluGly: 3.979 ± 0.577
1.257GluHis: 1.257 ± 0.375
5.864GluIle: 5.864 ± 0.827
5.446GluLys: 5.446 ± 0.816
8.483GluLeu: 8.483 ± 1.253
1.78GluMet: 1.78 ± 0.419
2.828GluAsn: 2.828 ± 0.631
1.466GluPro: 1.466 ± 0.412
3.037GluGln: 3.037 ± 0.436
3.142GluArg: 3.142 ± 0.572
3.037GluSer: 3.037 ± 0.562
3.246GluThr: 3.246 ± 0.64
4.294GluVal: 4.294 ± 0.859
0.314GluTrp: 0.314 ± 0.153
3.979GluTyr: 3.979 ± 0.71
0.0GluXaa: 0.0 ± 0.0
Phe
2.304PheAla: 2.304 ± 0.697
0.209PheCys: 0.209 ± 0.124
3.246PheAsp: 3.246 ± 0.612
2.828PheGlu: 2.828 ± 0.527
1.466PhePhe: 1.466 ± 0.352
3.561PheGly: 3.561 ± 0.74
0.524PheHis: 0.524 ± 0.267
1.885PheIle: 1.885 ± 0.477
4.608PheLys: 4.608 ± 0.658
2.513PheLeu: 2.513 ± 0.584
0.628PheMet: 0.628 ± 0.28
3.561PheAsn: 3.561 ± 0.699
0.838PhePro: 0.838 ± 0.337
0.733PheGln: 0.733 ± 0.228
1.99PheArg: 1.99 ± 0.467
2.723PheSer: 2.723 ± 0.496
2.094PheThr: 2.094 ± 0.381
2.304PheVal: 2.304 ± 0.536
0.209PheTrp: 0.209 ± 0.136
0.943PheTyr: 0.943 ± 0.37
0.0PheXaa: 0.0 ± 0.0
Gly
5.131GlyAla: 5.131 ± 1.212
0.0GlyCys: 0.0 ± 0.0
3.875GlyAsp: 3.875 ± 0.607
4.084GlyGlu: 4.084 ± 0.492
3.037GlyPhe: 3.037 ± 0.557
3.351GlyGly: 3.351 ± 0.658
1.047GlyHis: 1.047 ± 0.302
3.456GlyIle: 3.456 ± 0.605
6.598GlyLys: 6.598 ± 0.751
6.912GlyLeu: 6.912 ± 1.067
1.99GlyMet: 1.99 ± 0.356
3.037GlyAsn: 3.037 ± 0.579
2.199GlyPro: 2.199 ± 1.375
3.037GlyGln: 3.037 ± 0.638
2.932GlyArg: 2.932 ± 0.73
3.351GlySer: 3.351 ± 0.88
3.979GlyThr: 3.979 ± 0.776
4.189GlyVal: 4.189 ± 1.032
0.628GlyTrp: 0.628 ± 0.309
2.828GlyTyr: 2.828 ± 0.528
0.0GlyXaa: 0.0 ± 0.0
His
0.733HisAla: 0.733 ± 0.279
0.209HisCys: 0.209 ± 0.142
0.524HisAsp: 0.524 ± 0.243
1.466HisGlu: 1.466 ± 0.492
1.047HisPhe: 1.047 ± 0.284
0.733HisGly: 0.733 ± 0.23
0.419HisHis: 0.419 ± 0.263
0.943HisIle: 0.943 ± 0.242
1.047HisLys: 1.047 ± 0.324
0.733HisLeu: 0.733 ± 0.278
0.209HisMet: 0.209 ± 0.146
1.152HisAsn: 1.152 ± 0.397
0.419HisPro: 0.419 ± 0.164
0.628HisGln: 0.628 ± 0.235
0.628HisArg: 0.628 ± 0.264
0.943HisSer: 0.943 ± 0.29
0.838HisThr: 0.838 ± 0.313
1.047HisVal: 1.047 ± 0.381
0.209HisTrp: 0.209 ± 0.126
0.628HisTyr: 0.628 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
5.131IleAla: 5.131 ± 0.816
0.314IleCys: 0.314 ± 0.189
6.179IleAsp: 6.179 ± 0.909
5.236IleGlu: 5.236 ± 0.753
2.513IlePhe: 2.513 ± 0.653
3.77IleGly: 3.77 ± 0.955
1.361IleHis: 1.361 ± 0.413
5.027IleIle: 5.027 ± 0.541
7.854IleLys: 7.854 ± 1.018
3.77IleLeu: 3.77 ± 0.517
0.524IleMet: 0.524 ± 0.228
3.561IleAsn: 3.561 ± 0.751
1.571IlePro: 1.571 ± 0.325
2.618IleGln: 2.618 ± 0.45
2.618IleArg: 2.618 ± 0.528
4.608IleSer: 4.608 ± 1.027
5.446IleThr: 5.446 ± 0.584
3.979IleVal: 3.979 ± 0.536
0.209IleTrp: 0.209 ± 0.127
2.932IleTyr: 2.932 ± 0.616
0.0IleXaa: 0.0 ± 0.0
Lys
7.54LysAla: 7.54 ± 0.994
0.419LysCys: 0.419 ± 0.21
6.179LysAsp: 6.179 ± 0.689
6.388LysGlu: 6.388 ± 0.919
1.885LysPhe: 1.885 ± 0.457
6.493LysGly: 6.493 ± 0.87
1.676LysHis: 1.676 ± 0.409
6.702LysIle: 6.702 ± 0.679
7.331LysLys: 7.331 ± 1.398
8.378LysLeu: 8.378 ± 1.118
3.142LysMet: 3.142 ± 0.513
4.922LysAsn: 4.922 ± 0.711
1.78LysPro: 1.78 ± 0.532
4.503LysGln: 4.503 ± 0.542
4.294LysArg: 4.294 ± 0.697
5.446LysSer: 5.446 ± 0.729
5.969LysThr: 5.969 ± 0.779
6.074LysVal: 6.074 ± 0.995
0.838LysTrp: 0.838 ± 0.303
2.932LysTyr: 2.932 ± 0.632
0.0LysXaa: 0.0 ± 0.0
Leu
6.493LeuAla: 6.493 ± 0.92
0.105LeuCys: 0.105 ± 0.115
8.483LeuAsp: 8.483 ± 0.932
7.54LeuGlu: 7.54 ± 0.953
2.932LeuPhe: 2.932 ± 0.644
5.027LeuGly: 5.027 ± 0.915
1.257LeuHis: 1.257 ± 0.3
4.817LeuIle: 4.817 ± 0.651
7.54LeuLys: 7.54 ± 0.988
5.341LeuLeu: 5.341 ± 0.901
1.466LeuMet: 1.466 ± 0.449
4.294LeuAsn: 4.294 ± 0.78
2.304LeuPro: 2.304 ± 0.317
3.456LeuGln: 3.456 ± 0.851
3.665LeuArg: 3.665 ± 0.839
6.702LeuSer: 6.702 ± 0.771
4.817LeuThr: 4.817 ± 0.559
5.027LeuVal: 5.027 ± 0.531
0.524LeuTrp: 0.524 ± 0.241
2.618LeuTyr: 2.618 ± 0.655
0.0LeuXaa: 0.0 ± 0.0
Met
2.828MetAla: 2.828 ± 0.656
0.0MetCys: 0.0 ± 0.0
1.676MetAsp: 1.676 ± 0.337
1.361MetGlu: 1.361 ± 0.367
0.943MetPhe: 0.943 ± 0.308
1.676MetGly: 1.676 ± 0.454
0.105MetHis: 0.105 ± 0.097
2.513MetIle: 2.513 ± 0.534
1.152MetLys: 1.152 ± 0.433
1.885MetLeu: 1.885 ± 0.426
0.314MetMet: 0.314 ± 0.177
0.943MetAsn: 0.943 ± 0.27
0.524MetPro: 0.524 ± 0.201
1.047MetGln: 1.047 ± 0.284
1.257MetArg: 1.257 ± 0.33
1.885MetSer: 1.885 ± 0.352
1.676MetThr: 1.676 ± 0.387
1.047MetVal: 1.047 ± 0.319
0.0MetTrp: 0.0 ± 0.0
0.419MetTyr: 0.419 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.189AsnAla: 4.189 ± 0.677
0.419AsnCys: 0.419 ± 0.183
2.828AsnAsp: 2.828 ± 0.549
3.037AsnGlu: 3.037 ± 0.486
1.885AsnPhe: 1.885 ± 0.4
4.503AsnGly: 4.503 ± 0.635
1.466AsnHis: 1.466 ± 0.346
3.665AsnIle: 3.665 ± 0.831
4.503AsnLys: 4.503 ± 0.703
4.503AsnLeu: 4.503 ± 0.488
1.152AsnMet: 1.152 ± 0.323
2.094AsnAsn: 2.094 ± 0.495
1.152AsnPro: 1.152 ± 0.314
1.885AsnGln: 1.885 ± 0.408
1.676AsnArg: 1.676 ± 0.463
2.618AsnSer: 2.618 ± 0.617
2.723AsnThr: 2.723 ± 0.765
1.885AsnVal: 1.885 ± 0.511
0.524AsnTrp: 0.524 ± 0.204
1.571AsnTyr: 1.571 ± 0.355
0.0AsnXaa: 0.0 ± 0.0
Pro
1.676ProAla: 1.676 ± 0.409
0.209ProCys: 0.209 ± 0.122
1.78ProAsp: 1.78 ± 0.449
1.571ProGlu: 1.571 ± 0.467
1.152ProPhe: 1.152 ± 0.398
1.361ProGly: 1.361 ± 0.554
0.524ProHis: 0.524 ± 0.2
1.257ProIle: 1.257 ± 0.36
1.99ProLys: 1.99 ± 0.432
0.628ProLeu: 0.628 ± 0.284
0.524ProMet: 0.524 ± 0.23
1.047ProAsn: 1.047 ± 0.302
1.152ProPro: 1.152 ± 0.42
1.361ProGln: 1.361 ± 0.67
2.094ProArg: 2.094 ± 0.584
2.199ProSer: 2.199 ± 0.401
1.152ProThr: 1.152 ± 0.314
1.361ProVal: 1.361 ± 0.404
0.105ProTrp: 0.105 ± 0.117
0.943ProTyr: 0.943 ± 0.379
0.0ProXaa: 0.0 ± 0.0
Gln
3.665GlnAla: 3.665 ± 1.128
0.524GlnCys: 0.524 ± 0.262
1.571GlnAsp: 1.571 ± 0.427
3.037GlnGlu: 3.037 ± 0.466
0.733GlnPhe: 0.733 ± 0.23
3.456GlnGly: 3.456 ± 0.876
0.209GlnHis: 0.209 ± 0.152
2.513GlnIle: 2.513 ± 0.506
4.817GlnLys: 4.817 ± 0.784
3.875GlnLeu: 3.875 ± 0.688
0.838GlnMet: 0.838 ± 0.301
1.676GlnAsn: 1.676 ± 0.571
1.047GlnPro: 1.047 ± 0.303
1.676GlnGln: 1.676 ± 0.654
1.361GlnArg: 1.361 ± 0.39
4.608GlnSer: 4.608 ± 0.76
3.037GlnThr: 3.037 ± 0.587
1.99GlnVal: 1.99 ± 0.421
0.0GlnTrp: 0.0 ± 0.0
1.78GlnTyr: 1.78 ± 0.409
0.0GlnXaa: 0.0 ± 0.0
Arg
2.409ArgAla: 2.409 ± 0.519
0.419ArgCys: 0.419 ± 0.165
2.304ArgAsp: 2.304 ± 0.478
3.665ArgGlu: 3.665 ± 0.713
1.885ArgPhe: 1.885 ± 0.389
2.199ArgGly: 2.199 ± 0.486
0.314ArgHis: 0.314 ± 0.169
2.828ArgIle: 2.828 ± 0.456
4.713ArgLys: 4.713 ± 0.913
5.027ArgLeu: 5.027 ± 0.573
1.361ArgMet: 1.361 ± 0.396
1.885ArgAsn: 1.885 ± 0.452
0.943ArgPro: 0.943 ± 0.361
2.409ArgGln: 2.409 ± 0.462
2.094ArgArg: 2.094 ± 0.59
1.99ArgSer: 1.99 ± 0.429
1.361ArgThr: 1.361 ± 0.284
2.618ArgVal: 2.618 ± 0.717
0.419ArgTrp: 0.419 ± 0.191
2.199ArgTyr: 2.199 ± 0.482
0.0ArgXaa: 0.0 ± 0.0
Ser
5.655SerAla: 5.655 ± 1.584
0.105SerCys: 0.105 ± 0.104
4.294SerAsp: 4.294 ± 0.76
3.665SerGlu: 3.665 ± 0.728
2.828SerPhe: 2.828 ± 0.475
3.665SerGly: 3.665 ± 0.841
0.733SerHis: 0.733 ± 0.299
5.864SerIle: 5.864 ± 0.918
5.131SerLys: 5.131 ± 0.666
4.922SerLeu: 4.922 ± 0.807
2.409SerMet: 2.409 ± 0.655
2.409SerAsn: 2.409 ± 0.53
1.78SerPro: 1.78 ± 0.568
3.665SerGln: 3.665 ± 0.86
3.037SerArg: 3.037 ± 0.574
4.817SerSer: 4.817 ± 0.791
3.351SerThr: 3.351 ± 0.413
4.189SerVal: 4.189 ± 0.606
0.733SerTrp: 0.733 ± 0.335
2.409SerTyr: 2.409 ± 0.547
0.0SerXaa: 0.0 ± 0.0
Thr
4.084ThrAla: 4.084 ± 1.145
0.314ThrCys: 0.314 ± 0.202
3.77ThrAsp: 3.77 ± 0.666
3.665ThrGlu: 3.665 ± 0.724
3.665ThrPhe: 3.665 ± 0.538
4.398ThrGly: 4.398 ± 0.812
0.628ThrHis: 0.628 ± 0.268
3.77ThrIle: 3.77 ± 0.655
4.084ThrLys: 4.084 ± 0.701
6.179ThrLeu: 6.179 ± 0.855
0.838ThrMet: 0.838 ± 0.355
2.618ThrAsn: 2.618 ± 0.437
1.571ThrPro: 1.571 ± 0.41
1.99ThrGln: 1.99 ± 0.303
1.885ThrArg: 1.885 ± 0.421
3.246ThrSer: 3.246 ± 0.569
4.189ThrThr: 4.189 ± 0.843
5.655ThrVal: 5.655 ± 0.818
0.628ThrTrp: 0.628 ± 0.205
1.99ThrTyr: 1.99 ± 0.496
0.0ThrXaa: 0.0 ± 0.0
Val
3.979ValAla: 3.979 ± 1.042
0.524ValCys: 0.524 ± 0.286
4.713ValAsp: 4.713 ± 0.815
3.351ValGlu: 3.351 ± 0.569
2.723ValPhe: 2.723 ± 0.604
4.084ValGly: 4.084 ± 0.896
0.628ValHis: 0.628 ± 0.204
3.665ValIle: 3.665 ± 0.798
5.969ValLys: 5.969 ± 0.872
4.189ValLeu: 4.189 ± 0.627
1.257ValMet: 1.257 ± 0.424
3.037ValAsn: 3.037 ± 0.367
1.361ValPro: 1.361 ± 0.414
3.351ValGln: 3.351 ± 0.51
2.409ValArg: 2.409 ± 0.439
4.817ValSer: 4.817 ± 0.83
3.665ValThr: 3.665 ± 0.653
4.817ValVal: 4.817 ± 0.626
0.733ValTrp: 0.733 ± 0.23
2.828ValTyr: 2.828 ± 0.564
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.0TrpCys: 0.0 ± 0.0
1.047TrpAsp: 1.047 ± 0.291
0.628TrpGlu: 0.628 ± 0.296
0.524TrpPhe: 0.524 ± 0.308
0.943TrpGly: 0.943 ± 0.372
0.209TrpHis: 0.209 ± 0.136
0.628TrpIle: 0.628 ± 0.213
0.733TrpLys: 0.733 ± 0.253
0.419TrpLeu: 0.419 ± 0.17
0.105TrpMet: 0.105 ± 0.085
0.105TrpAsn: 0.105 ± 0.115
0.105TrpPro: 0.105 ± 0.1
0.314TrpGln: 0.314 ± 0.164
0.314TrpArg: 0.314 ± 0.195
0.838TrpSer: 0.838 ± 0.271
0.419TrpThr: 0.419 ± 0.314
0.209TrpVal: 0.209 ± 0.123
0.0TrpTrp: 0.0 ± 0.0
0.209TrpTyr: 0.209 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.723TyrAla: 2.723 ± 0.554
0.419TyrCys: 0.419 ± 0.263
3.875TyrAsp: 3.875 ± 0.783
3.246TyrGlu: 3.246 ± 0.615
1.152TyrPhe: 1.152 ± 0.398
1.99TyrGly: 1.99 ± 0.351
0.733TyrHis: 0.733 ± 0.426
2.304TyrIle: 2.304 ± 0.439
4.084TyrLys: 4.084 ± 0.635
3.351TyrLeu: 3.351 ± 0.839
0.628TyrMet: 0.628 ± 0.25
2.513TyrAsn: 2.513 ± 0.685
0.838TyrPro: 0.838 ± 0.316
2.409TyrGln: 2.409 ± 0.584
1.361TyrArg: 1.361 ± 0.358
1.571TyrSer: 1.571 ± 0.366
2.618TyrThr: 2.618 ± 0.504
1.676TyrVal: 1.676 ± 0.416
0.314TyrTrp: 0.314 ± 0.182
1.676TyrTyr: 1.676 ± 0.482
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 37 proteins (9550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski