Peptoclostridium litorale DSM 5388

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptoclostridium; Peptoclostridium litorale

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A069RD74|A0A069RD74_PEPLI DNA topoisomerase 1 OS=Peptoclostridium litorale DSM 5388 OX=1121324 GN=topA PE=3 SV=1
MM1 pKa = 7.44GFAKK5 pKa = 10.0RR6 pKa = 11.84VITALVFSLLTAASSFAQLPNGSIVIYY33 pKa = 10.82NEE35 pKa = 4.65GYY37 pKa = 10.61DD38 pKa = 3.36ISLLFNSDD46 pKa = 2.57YY47 pKa = 11.14DD48 pKa = 3.88AQINAAASSHH58 pKa = 6.8PEE60 pKa = 3.55NIYY63 pKa = 11.15YY64 pKa = 10.34NISGTWTEE72 pKa = 4.17VFSGEE77 pKa = 3.97TAEE80 pKa = 5.96DD81 pKa = 4.09FKK83 pKa = 11.41SWPQIQYY90 pKa = 10.56NDD92 pKa = 3.75PDD94 pKa = 3.88GFTRR98 pKa = 11.84LYY100 pKa = 10.6GPQNGSPIQSDD111 pKa = 4.37LLWSLSGEE119 pKa = 4.08ILIDD123 pKa = 4.61GPDD126 pKa = 3.9GDD128 pKa = 5.03PDD130 pKa = 3.83SEE132 pKa = 4.52NYY134 pKa = 9.31MLLKK138 pKa = 10.36FNKK141 pKa = 9.34PLAQGDD147 pKa = 4.24YY148 pKa = 10.65TSLIDD153 pKa = 5.59DD154 pKa = 4.98LGSGQFLSVNNDD166 pKa = 2.44IVLSNISAVEE176 pKa = 3.88WSPEE180 pKa = 4.12SPDD183 pKa = 3.61EE184 pKa = 4.0LVLYY188 pKa = 10.61VSGAANIPSPATISFKK204 pKa = 11.2LKK206 pKa = 10.18GGLIKK211 pKa = 10.95GQDD214 pKa = 3.5GSALSQEE221 pKa = 4.25YY222 pKa = 10.65LGVSKK227 pKa = 9.41TVFNSHH233 pKa = 6.11YY234 pKa = 11.08GEE236 pKa = 4.26

Molecular weight: 25.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A069RHL2|A0A069RHL2_PEPLI ABC-type cobalamin/Fe3+-siderophores transport system ATPase component OS=Peptoclostridium litorale DSM 5388 OX=1121324 GN=CLIT_2c01070 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.74RR21 pKa = 11.84MKK23 pKa = 9.63SSNGKK28 pKa = 10.04RR29 pKa = 11.84ILKK32 pKa = 9.68KK33 pKa = 9.73RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.25

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2948

0

2948

924342

29

2827

313.5

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.802 ± 0.057

1.131 ± 0.019

5.806 ± 0.041

7.881 ± 0.055

4.248 ± 0.034

7.409 ± 0.041

1.571 ± 0.019

8.887 ± 0.049

7.85 ± 0.044

8.228 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.31 ± 0.026

4.529 ± 0.027

2.984 ± 0.023

2.545 ± 0.02

4.08 ± 0.031

6.929 ± 0.045

4.382 ± 0.032

7.083 ± 0.041

0.677 ± 0.015

3.667 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski