Amino acid dipepetide frequency for Lactococcus virus CB20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.815AlaAla: 0.815 ± 0.568
0.116AlaCys: 0.116 ± 0.129
2.795AlaAsp: 2.795 ± 0.657
4.774AlaGlu: 4.774 ± 0.779
3.493AlaPhe: 3.493 ± 1.044
4.308AlaGly: 4.308 ± 0.62
0.932AlaHis: 0.932 ± 0.289
4.891AlaIle: 4.891 ± 0.982
6.171AlaLys: 6.171 ± 0.922
6.171AlaLeu: 6.171 ± 1.034
2.678AlaMet: 2.678 ± 0.656
4.541AlaAsn: 4.541 ± 1.023
0.932AlaPro: 0.932 ± 0.376
2.678AlaGln: 2.678 ± 0.559
1.98AlaArg: 1.98 ± 0.394
3.493AlaSer: 3.493 ± 0.922
4.308AlaThr: 4.308 ± 0.934
3.377AlaVal: 3.377 ± 0.696
2.212AlaTrp: 2.212 ± 1.137
2.096AlaTyr: 2.096 ± 0.561
0.0AlaXaa: 0.0 ± 0.0
Cys
0.233CysAla: 0.233 ± 0.179
0.116CysCys: 0.116 ± 0.117
0.466CysAsp: 0.466 ± 0.229
0.349CysGlu: 0.349 ± 0.206
0.233CysPhe: 0.233 ± 0.169
0.815CysGly: 0.815 ± 0.374
0.233CysHis: 0.233 ± 0.16
0.349CysIle: 0.349 ± 0.187
0.815CysLys: 0.815 ± 0.386
0.582CysLeu: 0.582 ± 0.288
0.0CysMet: 0.0 ± 0.0
0.582CysAsn: 0.582 ± 0.276
0.349CysPro: 0.349 ± 0.22
0.233CysGln: 0.233 ± 0.16
0.582CysArg: 0.582 ± 0.275
0.116CysSer: 0.116 ± 0.125
0.233CysThr: 0.233 ± 0.146
0.466CysVal: 0.466 ± 0.254
0.116CysTrp: 0.116 ± 0.104
0.233CysTyr: 0.233 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
2.212AspAla: 2.212 ± 0.893
0.233AspCys: 0.233 ± 0.157
4.192AspAsp: 4.192 ± 0.936
4.075AspGlu: 4.075 ± 1.046
4.308AspPhe: 4.308 ± 0.613
3.959AspGly: 3.959 ± 0.629
0.932AspHis: 0.932 ± 0.367
4.192AspIle: 4.192 ± 0.841
4.774AspLys: 4.774 ± 0.683
6.521AspLeu: 6.521 ± 1.059
1.048AspMet: 1.048 ± 0.243
4.425AspAsn: 4.425 ± 0.746
1.397AspPro: 1.397 ± 0.464
0.699AspGln: 0.699 ± 0.395
1.747AspArg: 1.747 ± 0.42
2.795AspSer: 2.795 ± 0.564
3.959AspThr: 3.959 ± 0.742
2.795AspVal: 2.795 ± 0.559
0.932AspTrp: 0.932 ± 0.266
2.795AspTyr: 2.795 ± 0.633
0.0AspXaa: 0.0 ± 0.0
Glu
3.726GluAla: 3.726 ± 0.658
0.466GluCys: 0.466 ± 0.222
3.26GluAsp: 3.26 ± 0.846
4.541GluGlu: 4.541 ± 0.941
3.726GluPhe: 3.726 ± 0.597
2.445GluGly: 2.445 ± 0.529
0.699GluHis: 0.699 ± 0.329
5.123GluIle: 5.123 ± 0.863
5.24GluLys: 5.24 ± 1.092
9.781GluLeu: 9.781 ± 1.587
2.678GluMet: 2.678 ± 0.578
5.007GluAsn: 5.007 ± 0.749
1.164GluPro: 1.164 ± 0.386
3.493GluGln: 3.493 ± 0.781
2.096GluArg: 2.096 ± 0.42
4.075GluSer: 4.075 ± 0.488
4.774GluThr: 4.774 ± 0.675
3.959GluVal: 3.959 ± 0.717
0.932GluTrp: 0.932 ± 0.291
3.027GluTyr: 3.027 ± 0.814
0.0GluXaa: 0.0 ± 0.0
Phe
2.445PheAla: 2.445 ± 0.652
0.233PheCys: 0.233 ± 0.166
3.959PheAsp: 3.959 ± 0.696
3.144PheGlu: 3.144 ± 0.645
2.329PhePhe: 2.329 ± 0.826
2.096PheGly: 2.096 ± 0.379
0.116PheHis: 0.116 ± 0.114
3.377PheIle: 3.377 ± 0.769
3.843PheLys: 3.843 ± 0.735
2.329PheLeu: 2.329 ± 0.579
0.815PheMet: 0.815 ± 0.315
3.144PheAsn: 3.144 ± 0.907
0.815PhePro: 0.815 ± 0.307
1.514PheGln: 1.514 ± 0.503
1.048PheArg: 1.048 ± 0.255
4.658PheSer: 4.658 ± 1.358
3.493PheThr: 3.493 ± 0.597
3.027PheVal: 3.027 ± 0.522
0.349PheTrp: 0.349 ± 0.193
1.281PheTyr: 1.281 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
3.493GlyAla: 3.493 ± 1.255
0.233GlyCys: 0.233 ± 0.149
3.726GlyAsp: 3.726 ± 0.542
3.843GlyGlu: 3.843 ± 0.55
2.795GlyPhe: 2.795 ± 0.619
4.308GlyGly: 4.308 ± 0.919
0.699GlyHis: 0.699 ± 0.273
4.891GlyIle: 4.891 ± 1.791
6.87GlyLys: 6.87 ± 0.771
5.473GlyLeu: 5.473 ± 1.265
1.281GlyMet: 1.281 ± 0.407
3.377GlyAsn: 3.377 ± 0.688
0.349GlyPro: 0.349 ± 0.254
2.096GlyGln: 2.096 ± 0.475
1.63GlyArg: 1.63 ± 0.381
4.658GlySer: 4.658 ± 0.824
3.726GlyThr: 3.726 ± 1.123
5.706GlyVal: 5.706 ± 1.051
1.048GlyTrp: 1.048 ± 0.317
3.26GlyTyr: 3.26 ± 0.66
0.0GlyXaa: 0.0 ± 0.0
His
0.932HisAla: 0.932 ± 0.276
0.582HisCys: 0.582 ± 0.319
0.466HisAsp: 0.466 ± 0.233
0.466HisGlu: 0.466 ± 0.215
0.466HisPhe: 0.466 ± 0.246
0.932HisGly: 0.932 ± 0.391
0.116HisHis: 0.116 ± 0.127
1.048HisIle: 1.048 ± 0.311
0.699HisLys: 0.699 ± 0.281
1.048HisLeu: 1.048 ± 0.405
0.233HisMet: 0.233 ± 0.254
1.397HisAsn: 1.397 ± 0.485
0.349HisPro: 0.349 ± 0.18
0.233HisGln: 0.233 ± 0.16
0.233HisArg: 0.233 ± 0.17
0.466HisSer: 0.466 ± 0.257
1.048HisThr: 1.048 ± 0.388
0.932HisVal: 0.932 ± 0.419
0.0HisTrp: 0.0 ± 0.0
0.466HisTyr: 0.466 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
5.007IleAla: 5.007 ± 0.729
0.233IleCys: 0.233 ± 0.182
4.192IleAsp: 4.192 ± 0.522
6.055IleGlu: 6.055 ± 0.956
2.911IlePhe: 2.911 ± 0.557
3.726IleGly: 3.726 ± 1.057
1.048IleHis: 1.048 ± 0.402
5.589IleIle: 5.589 ± 0.75
6.986IleLys: 6.986 ± 0.685
5.473IleLeu: 5.473 ± 1.088
2.096IleMet: 2.096 ± 0.623
5.822IleAsn: 5.822 ± 0.69
1.63IlePro: 1.63 ± 0.421
2.678IleGln: 2.678 ± 0.466
1.397IleArg: 1.397 ± 0.379
4.075IleSer: 4.075 ± 0.877
4.658IleThr: 4.658 ± 0.62
4.541IleVal: 4.541 ± 0.813
1.281IleTrp: 1.281 ± 0.408
2.678IleTyr: 2.678 ± 0.502
0.0IleXaa: 0.0 ± 0.0
Lys
7.452LysAla: 7.452 ± 1.034
0.233LysCys: 0.233 ± 0.182
4.541LysAsp: 4.541 ± 0.752
7.103LysGlu: 7.103 ± 1.337
2.678LysPhe: 2.678 ± 0.494
5.24LysGly: 5.24 ± 0.933
1.048LysHis: 1.048 ± 0.389
4.774LysIle: 4.774 ± 0.921
8.5LysLys: 8.5 ± 1.356
7.219LysLeu: 7.219 ± 0.807
2.911LysMet: 2.911 ± 0.445
4.425LysAsn: 4.425 ± 0.697
1.747LysPro: 1.747 ± 0.463
3.726LysGln: 3.726 ± 0.829
3.959LysArg: 3.959 ± 0.793
5.706LysSer: 5.706 ± 1.121
5.24LysThr: 5.24 ± 1.03
5.822LysVal: 5.822 ± 0.717
1.63LysTrp: 1.63 ± 0.444
3.377LysTyr: 3.377 ± 0.569
0.0LysXaa: 0.0 ± 0.0
Leu
5.473LeuAla: 5.473 ± 0.773
0.349LeuCys: 0.349 ± 0.164
4.658LeuAsp: 4.658 ± 0.636
4.774LeuGlu: 4.774 ± 0.757
3.843LeuPhe: 3.843 ± 0.78
4.658LeuGly: 4.658 ± 1.009
1.397LeuHis: 1.397 ± 0.425
7.336LeuIle: 7.336 ± 1.064
8.5LeuLys: 8.5 ± 1.076
7.802LeuLeu: 7.802 ± 1.601
2.212LeuMet: 2.212 ± 0.615
5.356LeuAsn: 5.356 ± 1.037
3.144LeuPro: 3.144 ± 0.571
3.027LeuGln: 3.027 ± 0.484
2.795LeuArg: 2.795 ± 0.599
4.774LeuSer: 4.774 ± 0.723
6.288LeuThr: 6.288 ± 0.815
6.404LeuVal: 6.404 ± 0.926
1.514LeuTrp: 1.514 ± 0.366
4.658LeuTyr: 4.658 ± 0.956
0.0LeuXaa: 0.0 ± 0.0
Met
2.096MetAla: 2.096 ± 0.506
0.116MetCys: 0.116 ± 0.133
1.281MetAsp: 1.281 ± 0.353
1.747MetGlu: 1.747 ± 0.494
0.815MetPhe: 0.815 ± 0.406
1.048MetGly: 1.048 ± 0.353
0.466MetHis: 0.466 ± 0.244
2.445MetIle: 2.445 ± 0.556
2.329MetLys: 2.329 ± 0.544
2.096MetLeu: 2.096 ± 0.728
0.582MetMet: 0.582 ± 0.28
1.863MetAsn: 1.863 ± 0.508
0.932MetPro: 0.932 ± 0.353
1.514MetGln: 1.514 ± 0.399
0.466MetArg: 0.466 ± 0.218
1.63MetSer: 1.63 ± 0.39
1.863MetThr: 1.863 ± 0.516
1.397MetVal: 1.397 ± 0.379
0.349MetTrp: 0.349 ± 0.189
1.164MetTyr: 1.164 ± 0.381
0.0MetXaa: 0.0 ± 0.0
Asn
5.123AsnAla: 5.123 ± 1.175
0.233AsnCys: 0.233 ± 0.152
4.658AsnAsp: 4.658 ± 0.898
3.959AsnGlu: 3.959 ± 0.777
1.63AsnPhe: 1.63 ± 0.379
6.171AsnGly: 6.171 ± 0.738
1.164AsnHis: 1.164 ± 0.294
4.774AsnIle: 4.774 ± 0.754
6.288AsnLys: 6.288 ± 1.032
6.754AsnLeu: 6.754 ± 1.097
1.281AsnMet: 1.281 ± 0.359
3.493AsnAsn: 3.493 ± 0.895
2.445AsnPro: 2.445 ± 0.544
1.863AsnGln: 1.863 ± 0.44
1.514AsnArg: 1.514 ± 0.386
4.774AsnSer: 4.774 ± 0.639
3.843AsnThr: 3.843 ± 0.738
3.144AsnVal: 3.144 ± 0.532
1.397AsnTrp: 1.397 ± 0.406
2.445AsnTyr: 2.445 ± 0.643
0.0AsnXaa: 0.0 ± 0.0
Pro
1.863ProAla: 1.863 ± 0.518
0.116ProCys: 0.116 ± 0.132
1.747ProAsp: 1.747 ± 0.498
1.98ProGlu: 1.98 ± 0.564
0.932ProPhe: 0.932 ± 0.334
0.349ProGly: 0.349 ± 0.211
0.0ProHis: 0.0 ± 0.0
1.863ProIle: 1.863 ± 0.494
2.096ProLys: 2.096 ± 0.534
1.397ProLeu: 1.397 ± 0.351
0.582ProMet: 0.582 ± 0.252
2.678ProAsn: 2.678 ± 0.75
0.582ProPro: 0.582 ± 0.276
0.582ProGln: 0.582 ± 0.308
0.699ProArg: 0.699 ± 0.265
0.932ProSer: 0.932 ± 0.335
2.445ProThr: 2.445 ± 0.391
1.747ProVal: 1.747 ± 0.551
0.349ProTrp: 0.349 ± 0.216
0.815ProTyr: 0.815 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
3.493GlnAla: 3.493 ± 0.762
0.233GlnCys: 0.233 ± 0.172
1.63GlnAsp: 1.63 ± 0.436
2.445GlnGlu: 2.445 ± 0.462
1.048GlnPhe: 1.048 ± 0.372
2.795GlnGly: 2.795 ± 0.617
0.349GlnHis: 0.349 ± 0.192
1.281GlnIle: 1.281 ± 0.35
2.911GlnLys: 2.911 ± 0.785
3.26GlnLeu: 3.26 ± 0.615
0.815GlnMet: 0.815 ± 0.268
1.863GlnAsn: 1.863 ± 0.496
1.281GlnPro: 1.281 ± 0.398
1.397GlnGln: 1.397 ± 0.458
1.514GlnArg: 1.514 ± 0.488
1.863GlnSer: 1.863 ± 0.461
2.678GlnThr: 2.678 ± 0.44
2.212GlnVal: 2.212 ± 0.477
0.699GlnTrp: 0.699 ± 0.217
1.514GlnTyr: 1.514 ± 0.395
0.0GlnXaa: 0.0 ± 0.0
Arg
1.98ArgAla: 1.98 ± 0.535
0.466ArgCys: 0.466 ± 0.236
1.747ArgAsp: 1.747 ± 0.455
1.98ArgGlu: 1.98 ± 0.483
0.815ArgPhe: 0.815 ± 0.354
2.212ArgGly: 2.212 ± 0.441
0.582ArgHis: 0.582 ± 0.293
2.445ArgIle: 2.445 ± 0.527
3.726ArgLys: 3.726 ± 0.924
3.027ArgLeu: 3.027 ± 0.562
0.932ArgMet: 0.932 ± 0.41
2.445ArgAsn: 2.445 ± 0.652
0.466ArgPro: 0.466 ± 0.25
1.397ArgGln: 1.397 ± 0.333
1.863ArgArg: 1.863 ± 0.517
1.747ArgSer: 1.747 ± 0.37
1.514ArgThr: 1.514 ± 0.43
1.747ArgVal: 1.747 ± 0.451
0.349ArgTrp: 0.349 ± 0.207
1.63ArgTyr: 1.63 ± 0.457
0.0ArgXaa: 0.0 ± 0.0
Ser
5.123SerAla: 5.123 ± 1.444
0.815SerCys: 0.815 ± 0.388
3.959SerAsp: 3.959 ± 0.751
3.377SerGlu: 3.377 ± 0.551
2.911SerPhe: 2.911 ± 0.816
5.822SerGly: 5.822 ± 1.801
0.466SerHis: 0.466 ± 0.234
4.774SerIle: 4.774 ± 0.825
3.959SerLys: 3.959 ± 0.819
5.706SerLeu: 5.706 ± 0.836
1.863SerMet: 1.863 ± 0.322
3.843SerAsn: 3.843 ± 0.779
1.164SerPro: 1.164 ± 0.384
1.747SerGln: 1.747 ± 0.498
2.212SerArg: 2.212 ± 0.448
5.123SerSer: 5.123 ± 1.161
3.144SerThr: 3.144 ± 0.69
4.541SerVal: 4.541 ± 0.861
0.699SerTrp: 0.699 ± 0.292
1.98SerTyr: 1.98 ± 0.484
0.0SerXaa: 0.0 ± 0.0
Thr
5.007ThrAla: 5.007 ± 0.796
0.466ThrCys: 0.466 ± 0.265
3.493ThrAsp: 3.493 ± 0.641
5.706ThrGlu: 5.706 ± 0.691
3.027ThrPhe: 3.027 ± 0.751
5.24ThrGly: 5.24 ± 0.606
0.116ThrHis: 0.116 ± 0.126
4.541ThrIle: 4.541 ± 0.653
4.658ThrLys: 4.658 ± 0.56
5.822ThrLeu: 5.822 ± 1.001
1.164ThrMet: 1.164 ± 0.321
4.192ThrAsn: 4.192 ± 0.613
2.096ThrPro: 2.096 ± 0.354
2.562ThrGln: 2.562 ± 0.556
2.445ThrArg: 2.445 ± 0.514
4.541ThrSer: 4.541 ± 0.915
3.843ThrThr: 3.843 ± 0.75
4.658ThrVal: 4.658 ± 0.635
0.699ThrTrp: 0.699 ± 0.297
2.212ThrTyr: 2.212 ± 0.525
0.0ThrXaa: 0.0 ± 0.0
Val
3.61ValAla: 3.61 ± 0.574
0.699ValCys: 0.699 ± 0.343
4.075ValAsp: 4.075 ± 0.789
5.356ValGlu: 5.356 ± 0.58
2.562ValPhe: 2.562 ± 0.505
3.493ValGly: 3.493 ± 0.556
0.466ValHis: 0.466 ± 0.226
4.425ValIle: 4.425 ± 0.608
5.706ValLys: 5.706 ± 0.541
3.61ValLeu: 3.61 ± 0.703
1.747ValMet: 1.747 ± 0.534
3.61ValAsn: 3.61 ± 0.651
1.63ValPro: 1.63 ± 0.511
1.514ValGln: 1.514 ± 0.459
3.26ValArg: 3.26 ± 0.724
5.007ValSer: 5.007 ± 1.01
5.706ValThr: 5.706 ± 1.04
3.726ValVal: 3.726 ± 1.027
0.466ValTrp: 0.466 ± 0.218
2.096ValTyr: 2.096 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.815TrpAla: 0.815 ± 0.339
0.582TrpCys: 0.582 ± 0.304
1.048TrpAsp: 1.048 ± 0.476
0.815TrpGlu: 0.815 ± 0.293
1.164TrpPhe: 1.164 ± 0.488
0.699TrpGly: 0.699 ± 0.234
0.233TrpHis: 0.233 ± 0.203
0.582TrpIle: 0.582 ± 0.259
0.699TrpLys: 0.699 ± 0.25
1.863TrpLeu: 1.863 ± 0.453
0.466TrpMet: 0.466 ± 0.203
1.514TrpAsn: 1.514 ± 0.432
0.0TrpPro: 0.0 ± 0.0
0.932TrpGln: 0.932 ± 0.327
0.582TrpArg: 0.582 ± 0.297
1.164TrpSer: 1.164 ± 0.29
0.815TrpThr: 0.815 ± 0.284
0.582TrpVal: 0.582 ± 0.296
0.116TrpTrp: 0.116 ± 0.114
0.932TrpTyr: 0.932 ± 0.345
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.863TyrAla: 1.863 ± 0.581
0.582TyrCys: 0.582 ± 0.408
2.096TyrAsp: 2.096 ± 0.674
3.726TyrGlu: 3.726 ± 0.909
2.329TyrPhe: 2.329 ± 0.507
3.144TyrGly: 3.144 ± 0.63
1.048TyrHis: 1.048 ± 0.383
3.377TyrIle: 3.377 ± 0.762
2.678TyrLys: 2.678 ± 0.561
3.027TyrLeu: 3.027 ± 0.747
0.699TyrMet: 0.699 ± 0.296
3.377TyrAsn: 3.377 ± 0.614
1.281TyrPro: 1.281 ± 0.419
1.397TyrGln: 1.397 ± 0.573
1.048TyrArg: 1.048 ± 0.385
1.63TyrSer: 1.63 ± 0.439
2.911TyrThr: 2.911 ± 0.723
1.98TyrVal: 1.98 ± 0.472
0.466TyrTrp: 0.466 ± 0.212
1.98TyrTyr: 1.98 ± 0.55
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (8589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski