Amino acid dipepetide frequency for Mycobacterium phage Shauna1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.65AlaAla: 14.65 ± 1.732
1.046AlaCys: 1.046 ± 0.251
7.534AlaAsp: 7.534 ± 0.672
7.743AlaGlu: 7.743 ± 0.776
2.564AlaPhe: 2.564 ± 0.366
10.516AlaGly: 10.516 ± 1.166
2.564AlaHis: 2.564 ± 0.361
4.709AlaIle: 4.709 ± 0.704
4.081AlaLys: 4.081 ± 0.422
8.057AlaLeu: 8.057 ± 0.741
2.668AlaMet: 2.668 ± 0.353
2.721AlaAsn: 2.721 ± 0.338
5.18AlaPro: 5.18 ± 0.52
3.819AlaGln: 3.819 ± 0.408
7.482AlaArg: 7.482 ± 0.807
4.343AlaSer: 4.343 ± 0.381
5.075AlaThr: 5.075 ± 0.532
7.743AlaVal: 7.743 ± 0.667
2.93AlaTrp: 2.93 ± 0.385
2.197AlaTyr: 2.197 ± 0.291
0.0AlaXaa: 0.0 ± 0.0
Cys
1.151CysAla: 1.151 ± 0.33
0.157CysCys: 0.157 ± 0.093
1.413CysAsp: 1.413 ± 0.342
0.837CysGlu: 0.837 ± 0.205
0.209CysPhe: 0.209 ± 0.09
1.622CysGly: 1.622 ± 0.423
0.366CysHis: 0.366 ± 0.147
0.419CysIle: 0.419 ± 0.17
0.366CysLys: 0.366 ± 0.125
0.785CysLeu: 0.785 ± 0.225
0.366CysMet: 0.366 ± 0.132
0.471CysAsn: 0.471 ± 0.146
0.994CysPro: 0.994 ± 0.252
0.366CysGln: 0.366 ± 0.115
0.837CysArg: 0.837 ± 0.236
0.785CysSer: 0.785 ± 0.2
0.837CysThr: 0.837 ± 0.261
0.628CysVal: 0.628 ± 0.179
0.314CysTrp: 0.314 ± 0.137
0.157CysTyr: 0.157 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
7.429AspAla: 7.429 ± 0.597
1.046AspCys: 1.046 ± 0.233
4.604AspAsp: 4.604 ± 0.523
3.401AspGlu: 3.401 ± 0.439
2.25AspPhe: 2.25 ± 0.273
7.377AspGly: 7.377 ± 0.674
1.413AspHis: 1.413 ± 0.254
2.668AspIle: 2.668 ± 0.357
1.831AspLys: 1.831 ± 0.286
5.965AspLeu: 5.965 ± 0.561
0.942AspMet: 0.942 ± 0.207
0.994AspAsn: 0.994 ± 0.24
4.186AspPro: 4.186 ± 0.525
2.459AspGln: 2.459 ± 0.357
5.075AspArg: 5.075 ± 0.434
3.61AspSer: 3.61 ± 0.5
4.186AspThr: 4.186 ± 0.479
4.5AspVal: 4.5 ± 0.501
1.465AspTrp: 1.465 ± 0.276
1.779AspTyr: 1.779 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
6.331GluAla: 6.331 ± 0.54
0.732GluCys: 0.732 ± 0.23
3.349GluAsp: 3.349 ± 0.351
3.349GluGlu: 3.349 ± 0.541
1.988GluPhe: 1.988 ± 0.353
2.825GluGly: 2.825 ± 0.325
1.57GluHis: 1.57 ± 0.335
2.302GluIle: 2.302 ± 0.402
1.884GluLys: 1.884 ± 0.254
5.18GluLeu: 5.18 ± 0.627
2.04GluMet: 2.04 ± 0.291
1.936GluAsn: 1.936 ± 0.253
3.505GluPro: 3.505 ± 0.517
2.825GluGln: 2.825 ± 0.422
5.127GluArg: 5.127 ± 0.572
2.459GluSer: 2.459 ± 0.398
5.18GluThr: 5.18 ± 0.67
4.395GluVal: 4.395 ± 0.572
1.413GluTrp: 1.413 ± 0.246
1.622GluTyr: 1.622 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
3.244PheAla: 3.244 ± 0.377
0.209PheCys: 0.209 ± 0.105
2.721PheAsp: 2.721 ± 0.401
1.517PheGlu: 1.517 ± 0.317
0.523PhePhe: 0.523 ± 0.165
2.773PheGly: 2.773 ± 0.465
0.314PheHis: 0.314 ± 0.122
1.36PheIle: 1.36 ± 0.314
0.994PheLys: 0.994 ± 0.232
1.831PheLeu: 1.831 ± 0.297
0.994PheMet: 0.994 ± 0.254
0.837PheAsn: 0.837 ± 0.263
1.36PhePro: 1.36 ± 0.261
0.785PheGln: 0.785 ± 0.302
1.465PheArg: 1.465 ± 0.297
1.465PheSer: 1.465 ± 0.264
2.145PheThr: 2.145 ± 0.378
1.936PheVal: 1.936 ± 0.27
0.471PheTrp: 0.471 ± 0.168
0.994PheTyr: 0.994 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
8.267GlyAla: 8.267 ± 0.925
1.099GlyCys: 1.099 ± 0.311
5.965GlyAsp: 5.965 ± 0.521
4.133GlyGlu: 4.133 ± 0.56
2.459GlyPhe: 2.459 ± 0.326
10.464GlyGly: 10.464 ± 1.706
1.831GlyHis: 1.831 ± 0.279
3.924GlyIle: 3.924 ± 0.42
2.616GlyLys: 2.616 ± 0.422
5.703GlyLeu: 5.703 ± 0.554
2.093GlyMet: 2.093 ± 0.356
2.721GlyAsn: 2.721 ± 0.376
4.447GlyPro: 4.447 ± 0.516
2.773GlyGln: 2.773 ± 0.518
5.127GlyArg: 5.127 ± 0.556
6.383GlySer: 6.383 ± 0.876
6.435GlyThr: 6.435 ± 0.761
6.226GlyVal: 6.226 ± 0.591
2.459GlyTrp: 2.459 ± 0.316
2.145GlyTyr: 2.145 ± 0.403
0.0GlyXaa: 0.0 ± 0.0
His
1.57HisAla: 1.57 ± 0.292
0.471HisCys: 0.471 ± 0.153
0.785HisAsp: 0.785 ± 0.179
1.413HisGlu: 1.413 ± 0.271
0.68HisPhe: 0.68 ± 0.206
1.831HisGly: 1.831 ± 0.339
0.889HisHis: 0.889 ± 0.274
1.517HisIle: 1.517 ± 0.319
0.889HisLys: 0.889 ± 0.193
1.517HisLeu: 1.517 ± 0.268
0.523HisMet: 0.523 ± 0.141
0.732HisAsn: 0.732 ± 0.153
1.884HisPro: 1.884 ± 0.281
0.628HisGln: 0.628 ± 0.176
2.354HisArg: 2.354 ± 0.381
0.523HisSer: 0.523 ± 0.152
1.413HisThr: 1.413 ± 0.303
1.256HisVal: 1.256 ± 0.305
0.366HisTrp: 0.366 ± 0.127
0.68HisTyr: 0.68 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
5.337IleAla: 5.337 ± 0.509
0.785IleCys: 0.785 ± 0.243
4.29IleAsp: 4.29 ± 0.541
3.715IleGlu: 3.715 ± 0.408
0.994IlePhe: 0.994 ± 0.236
3.192IleGly: 3.192 ± 0.39
1.36IleHis: 1.36 ± 0.233
1.413IleIle: 1.413 ± 0.248
0.942IleLys: 0.942 ± 0.231
2.04IleLeu: 2.04 ± 0.369
0.262IleMet: 0.262 ± 0.101
1.988IleAsn: 1.988 ± 0.312
3.244IlePro: 3.244 ± 0.366
1.308IleGln: 1.308 ± 0.216
2.982IleArg: 2.982 ± 0.436
1.936IleSer: 1.936 ± 0.405
3.976IleThr: 3.976 ± 0.531
2.564IleVal: 2.564 ± 0.321
0.785IleTrp: 0.785 ± 0.205
0.785IleTyr: 0.785 ± 0.213
0.0IleXaa: 0.0 ± 0.0
Lys
3.767LysAla: 3.767 ± 0.402
0.366LysCys: 0.366 ± 0.166
1.465LysAsp: 1.465 ± 0.227
1.308LysGlu: 1.308 ± 0.288
1.203LysPhe: 1.203 ± 0.242
2.825LysGly: 2.825 ± 0.367
1.046LysHis: 1.046 ± 0.261
0.994LysIle: 0.994 ± 0.268
1.465LysLys: 1.465 ± 0.472
2.354LysLeu: 2.354 ± 0.424
0.576LysMet: 0.576 ± 0.167
0.889LysAsn: 0.889 ± 0.218
2.616LysPro: 2.616 ± 0.46
1.203LysGln: 1.203 ± 0.215
2.511LysArg: 2.511 ± 0.432
2.093LysSer: 2.093 ± 0.307
2.145LysThr: 2.145 ± 0.303
2.616LysVal: 2.616 ± 0.377
0.785LysTrp: 0.785 ± 0.19
1.046LysTyr: 1.046 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
7.586LeuAla: 7.586 ± 0.768
1.046LeuCys: 1.046 ± 0.27
5.075LeuAsp: 5.075 ± 0.691
3.505LeuGlu: 3.505 ± 0.448
2.093LeuPhe: 2.093 ± 0.271
5.127LeuGly: 5.127 ± 0.561
0.837LeuHis: 0.837 ± 0.224
3.035LeuIle: 3.035 ± 0.504
1.936LeuLys: 1.936 ± 0.302
5.232LeuLeu: 5.232 ± 0.628
1.727LeuMet: 1.727 ± 0.299
2.511LeuAsn: 2.511 ± 0.397
5.389LeuPro: 5.389 ± 0.574
2.668LeuGln: 2.668 ± 0.413
5.337LeuArg: 5.337 ± 0.677
5.232LeuSer: 5.232 ± 0.628
5.441LeuThr: 5.441 ± 0.585
4.97LeuVal: 4.97 ± 0.517
1.36LeuTrp: 1.36 ± 0.283
1.884LeuTyr: 1.884 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
2.093MetAla: 2.093 ± 0.344
0.314MetCys: 0.314 ± 0.143
1.413MetAsp: 1.413 ± 0.297
1.308MetGlu: 1.308 ± 0.252
0.785MetPhe: 0.785 ± 0.235
1.465MetGly: 1.465 ± 0.346
0.314MetHis: 0.314 ± 0.114
0.889MetIle: 0.889 ± 0.225
0.523MetLys: 0.523 ± 0.192
1.622MetLeu: 1.622 ± 0.266
0.732MetMet: 0.732 ± 0.215
0.994MetAsn: 0.994 ± 0.183
1.674MetPro: 1.674 ± 0.253
0.576MetGln: 0.576 ± 0.165
1.779MetArg: 1.779 ± 0.274
2.773MetSer: 2.773 ± 0.354
2.145MetThr: 2.145 ± 0.307
1.046MetVal: 1.046 ± 0.299
0.262MetTrp: 0.262 ± 0.115
0.471MetTyr: 0.471 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.505AsnAla: 3.505 ± 0.423
0.366AsnCys: 0.366 ± 0.129
1.36AsnAsp: 1.36 ± 0.263
1.622AsnGlu: 1.622 ± 0.302
0.576AsnPhe: 0.576 ± 0.21
4.186AsnGly: 4.186 ± 0.404
0.837AsnHis: 0.837 ± 0.205
1.413AsnIle: 1.413 ± 0.337
1.203AsnLys: 1.203 ± 0.276
2.721AsnLeu: 2.721 ± 0.363
0.471AsnMet: 0.471 ± 0.157
1.727AsnAsn: 1.727 ± 0.328
2.354AsnPro: 2.354 ± 0.325
1.151AsnGln: 1.151 ± 0.286
1.988AsnArg: 1.988 ± 0.275
1.517AsnSer: 1.517 ± 0.276
2.093AsnThr: 2.093 ± 0.336
1.988AsnVal: 1.988 ± 0.307
0.628AsnTrp: 0.628 ± 0.147
0.471AsnTyr: 0.471 ± 0.152
0.0AsnXaa: 0.0 ± 0.0
Pro
5.86ProAla: 5.86 ± 0.727
0.942ProCys: 0.942 ± 0.244
4.604ProAsp: 4.604 ± 0.56
4.133ProGlu: 4.133 ± 0.475
1.779ProPhe: 1.779 ± 0.338
6.435ProGly: 6.435 ± 0.662
1.674ProHis: 1.674 ± 0.284
2.302ProIle: 2.302 ± 0.287
2.25ProLys: 2.25 ± 0.377
4.238ProLeu: 4.238 ± 0.522
1.727ProMet: 1.727 ± 0.324
1.884ProAsn: 1.884 ± 0.316
3.192ProPro: 3.192 ± 0.507
2.145ProGln: 2.145 ± 0.368
3.505ProArg: 3.505 ± 0.5
2.511ProSer: 2.511 ± 0.343
3.767ProThr: 3.767 ± 0.502
4.813ProVal: 4.813 ± 0.616
1.046ProTrp: 1.046 ± 0.195
1.517ProTyr: 1.517 ± 0.29
0.0ProXaa: 0.0 ± 0.0
Gln
4.29GlnAla: 4.29 ± 0.584
0.314GlnCys: 0.314 ± 0.115
1.57GlnAsp: 1.57 ± 0.247
1.988GlnGlu: 1.988 ± 0.36
1.151GlnPhe: 1.151 ± 0.206
2.25GlnGly: 2.25 ± 0.394
0.785GlnHis: 0.785 ± 0.22
1.622GlnIle: 1.622 ± 0.275
1.046GlnLys: 1.046 ± 0.205
3.296GlnLeu: 3.296 ± 0.428
0.68GlnMet: 0.68 ± 0.199
0.837GlnAsn: 0.837 ± 0.212
2.145GlnPro: 2.145 ± 0.338
1.046GlnGln: 1.046 ± 0.264
2.407GlnArg: 2.407 ± 0.366
2.25GlnSer: 2.25 ± 0.324
1.465GlnThr: 1.465 ± 0.261
2.668GlnVal: 2.668 ± 0.292
0.785GlnTrp: 0.785 ± 0.196
0.942GlnTyr: 0.942 ± 0.264
0.0GlnXaa: 0.0 ± 0.0
Arg
6.854ArgAla: 6.854 ± 0.733
1.36ArgCys: 1.36 ± 0.294
4.604ArgAsp: 4.604 ± 0.473
5.232ArgGlu: 5.232 ± 0.706
2.145ArgPhe: 2.145 ± 0.366
3.976ArgGly: 3.976 ± 0.386
1.203ArgHis: 1.203 ± 0.285
3.558ArgIle: 3.558 ± 0.468
2.825ArgLys: 2.825 ± 0.404
5.441ArgLeu: 5.441 ± 0.634
2.407ArgMet: 2.407 ± 0.377
2.459ArgAsn: 2.459 ± 0.365
3.558ArgPro: 3.558 ± 0.409
2.197ArgGln: 2.197 ± 0.33
6.592ArgArg: 6.592 ± 0.982
4.343ArgSer: 4.343 ± 0.483
3.819ArgThr: 3.819 ± 0.518
5.127ArgVal: 5.127 ± 0.573
2.25ArgTrp: 2.25 ± 0.272
1.831ArgTyr: 1.831 ± 0.281
0.0ArgXaa: 0.0 ± 0.0
Ser
7.011SerAla: 7.011 ± 1.127
0.471SerCys: 0.471 ± 0.156
3.819SerAsp: 3.819 ± 0.411
2.93SerGlu: 2.93 ± 0.381
1.674SerPhe: 1.674 ± 0.282
6.121SerGly: 6.121 ± 0.83
0.837SerHis: 0.837 ± 0.149
3.087SerIle: 3.087 ± 0.368
2.407SerLys: 2.407 ± 0.414
3.715SerLeu: 3.715 ± 0.37
1.256SerMet: 1.256 ± 0.262
2.04SerAsn: 2.04 ± 0.401
2.93SerPro: 2.93 ± 0.391
1.413SerGln: 1.413 ± 0.237
4.133SerArg: 4.133 ± 0.434
3.715SerSer: 3.715 ± 0.521
3.349SerThr: 3.349 ± 0.421
4.29SerVal: 4.29 ± 0.471
1.099SerTrp: 1.099 ± 0.218
1.046SerTyr: 1.046 ± 0.207
0.0SerXaa: 0.0 ± 0.0
Thr
6.697ThrAla: 6.697 ± 0.625
0.68ThrCys: 0.68 ± 0.201
3.924ThrAsp: 3.924 ± 0.603
3.924ThrGlu: 3.924 ± 0.433
1.308ThrPhe: 1.308 ± 0.224
6.069ThrGly: 6.069 ± 0.719
1.622ThrHis: 1.622 ± 0.315
3.453ThrIle: 3.453 ± 0.372
2.093ThrLys: 2.093 ± 0.295
3.715ThrLeu: 3.715 ± 0.455
1.36ThrMet: 1.36 ± 0.249
2.459ThrAsn: 2.459 ± 0.37
5.127ThrPro: 5.127 ± 0.571
2.197ThrGln: 2.197 ± 0.337
4.029ThrArg: 4.029 ± 0.414
4.186ThrSer: 4.186 ± 0.509
5.598ThrThr: 5.598 ± 0.777
6.435ThrVal: 6.435 ± 0.694
1.099ThrTrp: 1.099 ± 0.222
2.093ThrTyr: 2.093 ± 0.344
0.0ThrXaa: 0.0 ± 0.0
Val
7.848ValAla: 7.848 ± 0.568
1.099ValCys: 1.099 ± 0.213
5.18ValAsp: 5.18 ± 0.567
5.127ValGlu: 5.127 ± 0.551
2.25ValPhe: 2.25 ± 0.328
5.18ValGly: 5.18 ± 0.642
1.256ValHis: 1.256 ± 0.253
3.192ValIle: 3.192 ± 0.477
2.407ValLys: 2.407 ± 0.318
5.18ValLeu: 5.18 ± 0.619
1.151ValMet: 1.151 ± 0.224
2.511ValAsn: 2.511 ± 0.41
3.819ValPro: 3.819 ± 0.35
2.302ValGln: 2.302 ± 0.374
4.813ValArg: 4.813 ± 0.662
4.866ValSer: 4.866 ± 0.509
5.337ValThr: 5.337 ± 0.477
6.174ValVal: 6.174 ± 0.772
1.674ValTrp: 1.674 ± 0.273
1.517ValTyr: 1.517 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
1.831TrpAla: 1.831 ± 0.315
0.157TrpCys: 0.157 ± 0.094
1.727TrpAsp: 1.727 ± 0.285
1.046TrpGlu: 1.046 ± 0.261
0.732TrpPhe: 0.732 ± 0.216
1.151TrpGly: 1.151 ± 0.268
0.628TrpHis: 0.628 ± 0.174
1.099TrpIle: 1.099 ± 0.233
0.837TrpLys: 0.837 ± 0.19
1.57TrpLeu: 1.57 ± 0.394
0.837TrpMet: 0.837 ± 0.204
0.471TrpAsn: 0.471 ± 0.131
1.099TrpPro: 1.099 ± 0.253
0.889TrpGln: 0.889 ± 0.246
2.145TrpArg: 2.145 ± 0.374
1.622TrpSer: 1.622 ± 0.318
1.831TrpThr: 1.831 ± 0.32
1.517TrpVal: 1.517 ± 0.314
0.628TrpTrp: 0.628 ± 0.173
0.471TrpTyr: 0.471 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.321
0.419TyrCys: 0.419 ± 0.128
1.779TyrAsp: 1.779 ± 0.402
1.779TyrGlu: 1.779 ± 0.28
0.576TyrPhe: 0.576 ± 0.158
1.884TyrGly: 1.884 ± 0.39
0.576TyrHis: 0.576 ± 0.163
0.942TyrIle: 0.942 ± 0.246
0.68TyrLys: 0.68 ± 0.208
1.831TyrLeu: 1.831 ± 0.276
0.314TyrMet: 0.314 ± 0.113
0.994TyrAsn: 0.994 ± 0.222
1.622TyrPro: 1.622 ± 0.247
0.785TyrGln: 0.785 ± 0.195
2.145TyrArg: 2.145 ± 0.345
0.732TyrSer: 0.732 ± 0.201
1.831TyrThr: 1.831 ± 0.303
1.988TyrVal: 1.988 ± 0.327
0.471TyrTrp: 0.471 ± 0.177
0.576TyrTyr: 0.576 ± 0.145
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (19114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski