Lake Sarah-associated circular virus-14

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA43|A0A126GA43_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-14 OX=1685740 PE=3 SV=1
MM1 pKa = 7.32PSSYY5 pKa = 10.36RR6 pKa = 11.84RR7 pKa = 11.84VVFTLNNYY15 pKa = 7.42TNEE18 pKa = 4.03ILTSICNYY26 pKa = 10.35AEE28 pKa = 4.34SNCRR32 pKa = 11.84YY33 pKa = 10.25AIIAKK38 pKa = 9.4EE39 pKa = 3.98VAPTTGTPHH48 pKa = 6.32LQGFLHH54 pKa = 6.57FKK56 pKa = 10.14NPKK59 pKa = 4.89THH61 pKa = 5.91KK62 pKa = 9.26TLGKK66 pKa = 9.57VLPGGHH72 pKa = 6.62FLHH75 pKa = 7.23AKK77 pKa = 10.44GSDD80 pKa = 3.44EE81 pKa = 4.79DD82 pKa = 4.05SQVYY86 pKa = 9.72CSKK89 pKa = 10.51EE90 pKa = 4.0DD91 pKa = 3.61PSPWEE96 pKa = 4.15FGTMCHH102 pKa = 5.63QGKK105 pKa = 10.26RR106 pKa = 11.84SDD108 pKa = 4.21LDD110 pKa = 3.84DD111 pKa = 6.27AILTLNEE118 pKa = 4.12SGGDD122 pKa = 3.63LKK124 pKa = 10.74RR125 pKa = 11.84VAMEE129 pKa = 3.56HH130 pKa = 5.93AGAYY134 pKa = 8.75VRR136 pKa = 11.84YY137 pKa = 8.97HH138 pKa = 6.85RR139 pKa = 11.84GFAAYY144 pKa = 9.29KK145 pKa = 10.44SLVCATAPRR154 pKa = 11.84DD155 pKa = 3.86FKK157 pKa = 10.99TKK159 pKa = 10.44LVVLFGPPGTGKK171 pKa = 8.03TRR173 pKa = 11.84AAYY176 pKa = 9.27EE177 pKa = 3.89LAGEE181 pKa = 4.22NPYY184 pKa = 10.43PKK186 pKa = 9.93PRR188 pKa = 11.84GEE190 pKa = 3.73WWDD193 pKa = 4.54GYY195 pKa = 10.68CGNNGVIIDD204 pKa = 5.2DD205 pKa = 4.44FYY207 pKa = 11.9GWLKK211 pKa = 10.53FDD213 pKa = 4.24EE214 pKa = 4.6LLKK217 pKa = 10.86ISDD220 pKa = 4.19RR221 pKa = 11.84YY222 pKa = 9.39PYY224 pKa = 10.07RR225 pKa = 11.84VPIKK229 pKa = 10.33GGYY232 pKa = 9.47EE233 pKa = 3.93NFCSKK238 pKa = 10.54IIIITSNIDD247 pKa = 2.61ISKK250 pKa = 8.93WYY252 pKa = 10.3KK253 pKa = 10.02FDD255 pKa = 4.38GYY257 pKa = 11.21DD258 pKa = 3.16PAALYY263 pKa = 9.93RR264 pKa = 11.84RR265 pKa = 11.84CTKK268 pKa = 10.08YY269 pKa = 10.51LRR271 pKa = 11.84CEE273 pKa = 3.93KK274 pKa = 10.79DD275 pKa = 3.54VFCSMEE281 pKa = 4.46DD282 pKa = 3.54DD283 pKa = 3.84YY284 pKa = 11.91LVKK287 pKa = 10.43INYY290 pKa = 8.84

Molecular weight: 33.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA43|A0A126GA43_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-14 OX=1685740 PE=3 SV=1
MM1 pKa = 7.76PRR3 pKa = 11.84TKK5 pKa = 9.96TYY7 pKa = 10.39RR8 pKa = 11.84KK9 pKa = 10.01KK10 pKa = 10.73SGKK13 pKa = 9.84KK14 pKa = 8.29YY15 pKa = 10.59NKK17 pKa = 9.27QKK19 pKa = 9.74TYY21 pKa = 10.38RR22 pKa = 11.84KK23 pKa = 9.69KK24 pKa = 10.9SYY26 pKa = 8.42GTKK29 pKa = 9.27KK30 pKa = 10.16KK31 pKa = 9.83SYY33 pKa = 10.11KK34 pKa = 8.88RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.68YY38 pKa = 10.4GNPVNQPMVSKK49 pKa = 10.71SLSAEE54 pKa = 3.67QAYY57 pKa = 10.9LKK59 pKa = 10.2MIMNPCEE66 pKa = 3.89SLLAYY71 pKa = 10.05GPSNGEE77 pKa = 3.72IEE79 pKa = 4.36SGYY82 pKa = 11.16LMRR85 pKa = 11.84TTYY88 pKa = 10.08RR89 pKa = 11.84VKK91 pKa = 10.27LHH93 pKa = 6.85RR94 pKa = 11.84IKK96 pKa = 10.84EE97 pKa = 4.11RR98 pKa = 11.84FNGYY102 pKa = 8.97VIWFPDD108 pKa = 3.5FTNAGIGSQVKK119 pKa = 10.31ANNPYY124 pKa = 10.43YY125 pKa = 10.32DD126 pKa = 4.16ANGTDD131 pKa = 3.76VKK133 pKa = 11.23NLTAGNCFVWEE144 pKa = 4.5CNDD147 pKa = 4.43AGAIPQNSTIGIAPATTSAYY167 pKa = 9.99YY168 pKa = 10.49GYY170 pKa = 10.86GFSDD174 pKa = 3.7LGDD177 pKa = 3.95GGSASSIKK185 pKa = 10.73DD186 pKa = 3.16PAYY189 pKa = 10.29NWASANIVSQIRR201 pKa = 11.84CLAACMTWQYY211 pKa = 11.29TGTLKK216 pKa = 10.12DD217 pKa = 3.59TAGQMCTVANITPYY231 pKa = 11.12QLMYY235 pKa = 11.34GNGGTDD241 pKa = 3.32NAPISISDD249 pKa = 3.96MISLNKK255 pKa = 10.37SYY257 pKa = 11.35DD258 pKa = 3.53RR259 pKa = 11.84IAVQKK264 pKa = 10.75YY265 pKa = 6.75EE266 pKa = 4.26VKK268 pKa = 9.2HH269 pKa = 5.46TPSTLGSLYY278 pKa = 10.42RR279 pKa = 11.84DD280 pKa = 3.25TDD282 pKa = 3.32KK283 pKa = 11.11PYY285 pKa = 10.39TDD287 pKa = 3.97AATGQTSKK295 pKa = 9.79TGDD298 pKa = 3.33TNGNCFVKK306 pKa = 10.66GNAVEE311 pKa = 4.33TVDD314 pKa = 3.67EE315 pKa = 4.78FGVGALTGTTPTTISLEE332 pKa = 3.93ASHH335 pKa = 6.93INGIGFCWTGIDD347 pKa = 3.36ATVAQNIEE355 pKa = 3.75IEE357 pKa = 4.16MTKK360 pKa = 9.93IFEE363 pKa = 4.23WTPSGKK369 pKa = 10.06YY370 pKa = 9.91LATSSNAVSRR380 pKa = 11.84PIMTTDD386 pKa = 3.65QIMSSLNNRR395 pKa = 11.84MPGWEE400 pKa = 4.14SRR402 pKa = 11.84HH403 pKa = 5.13QRR405 pKa = 11.84MTPAQVSAVSGQAGVPNGGG424 pKa = 3.31

Molecular weight: 46.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

714

290

424

357.0

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.283 ± 0.636

2.521 ± 0.548

5.182 ± 0.809

4.062 ± 0.656

3.081 ± 0.828

8.964 ± 0.406

1.681 ± 0.637

5.742 ± 0.133

7.423 ± 0.3

5.462 ± 1.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.674

5.882 ± 0.827

5.042 ± 0.281

2.661 ± 0.961

4.342 ± 0.491

7.283 ± 1.043

7.983 ± 1.457

4.762 ± 0.039

1.681 ± 0.026

6.443 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski