Amino acid dipepetide frequency for Isobaculum melis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.708AlaAla: 5.708 ± 0.113
0.523AlaCys: 0.523 ± 0.027
3.784AlaAsp: 3.784 ± 0.067
4.994AlaGlu: 4.994 ± 0.09
3.391AlaPhe: 3.391 ± 0.067
5.198AlaGly: 5.198 ± 0.098
1.329AlaHis: 1.329 ± 0.039
6.275AlaIle: 6.275 ± 0.08
5.153AlaLys: 5.153 ± 0.1
7.726AlaLeu: 7.726 ± 0.104
2.068AlaMet: 2.068 ± 0.051
2.889AlaAsn: 2.889 ± 0.059
2.205AlaPro: 2.205 ± 0.062
2.819AlaGln: 2.819 ± 0.065
2.198AlaArg: 2.198 ± 0.052
4.2AlaSer: 4.2 ± 0.07
4.857AlaThr: 4.857 ± 0.096
5.056AlaVal: 5.056 ± 0.089
0.58AlaTrp: 0.58 ± 0.028
2.758AlaTyr: 2.758 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.379CysAla: 0.379 ± 0.025
0.083CysCys: 0.083 ± 0.01
0.303CysAsp: 0.303 ± 0.023
0.341CysGlu: 0.341 ± 0.022
0.322CysPhe: 0.322 ± 0.023
0.524CysGly: 0.524 ± 0.026
0.176CysHis: 0.176 ± 0.014
0.456CysIle: 0.456 ± 0.024
0.272CysLys: 0.272 ± 0.02
0.735CysLeu: 0.735 ± 0.029
0.178CysMet: 0.178 ± 0.017
0.199CysAsn: 0.199 ± 0.015
0.244CysPro: 0.244 ± 0.018
0.309CysGln: 0.309 ± 0.021
0.213CysArg: 0.213 ± 0.017
0.402CysSer: 0.402 ± 0.024
0.386CysThr: 0.386 ± 0.02
0.408CysVal: 0.408 ± 0.024
0.045CysTrp: 0.045 ± 0.008
0.277CysTyr: 0.277 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.695AspAla: 3.695 ± 0.065
0.354AspCys: 0.354 ± 0.021
2.432AspAsp: 2.432 ± 0.065
4.289AspGlu: 4.289 ± 0.09
2.811AspPhe: 2.811 ± 0.062
3.386AspGly: 3.386 ± 0.071
0.993AspHis: 0.993 ± 0.038
3.969AspIle: 3.969 ± 0.086
3.229AspLys: 3.229 ± 0.075
5.122AspLeu: 5.122 ± 0.082
1.457AspMet: 1.457 ± 0.046
1.739AspAsn: 1.739 ± 0.055
1.72AspPro: 1.72 ± 0.044
2.194AspGln: 2.194 ± 0.052
1.543AspArg: 1.543 ± 0.041
2.678AspSer: 2.678 ± 0.051
2.88AspThr: 2.88 ± 0.069
3.519AspVal: 3.519 ± 0.079
0.537AspTrp: 0.537 ± 0.028
2.563AspTyr: 2.563 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
6.355GluAla: 6.355 ± 0.103
0.277GluCys: 0.277 ± 0.021
3.553GluAsp: 3.553 ± 0.076
6.46GluGlu: 6.46 ± 0.127
2.255GluPhe: 2.255 ± 0.053
3.783GluGly: 3.783 ± 0.078
1.284GluHis: 1.284 ± 0.039
5.861GluIle: 5.861 ± 0.094
6.903GluLys: 6.903 ± 0.094
6.662GluLeu: 6.662 ± 0.094
2.516GluMet: 2.516 ± 0.06
4.052GluAsn: 4.052 ± 0.069
1.918GluPro: 1.918 ± 0.053
3.733GluGln: 3.733 ± 0.079
2.799GluArg: 2.799 ± 0.073
3.224GluSer: 3.224 ± 0.072
4.348GluThr: 4.348 ± 0.078
5.217GluVal: 5.217 ± 0.099
0.669GluTrp: 0.669 ± 0.028
2.085GluTyr: 2.085 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.838PheAla: 2.838 ± 0.062
0.333PheCys: 0.333 ± 0.017
2.665PheAsp: 2.665 ± 0.062
3.027PheGlu: 3.027 ± 0.062
2.454PhePhe: 2.454 ± 0.072
3.111PheGly: 3.111 ± 0.063
0.811PheHis: 0.811 ± 0.033
3.789PheIle: 3.789 ± 0.078
2.74PheLys: 2.74 ± 0.059
4.387PheLeu: 4.387 ± 0.083
1.171PheMet: 1.171 ± 0.042
2.06PheAsn: 2.06 ± 0.048
1.565PhePro: 1.565 ± 0.045
1.588PheGln: 1.588 ± 0.048
1.198PheArg: 1.198 ± 0.044
3.566PheSer: 3.566 ± 0.066
2.617PheThr: 2.617 ± 0.072
3.165PheVal: 3.165 ± 0.074
0.414PheTrp: 0.414 ± 0.022
1.906PheTyr: 1.906 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.572GlyAla: 4.572 ± 0.096
0.502GlyCys: 0.502 ± 0.026
2.944GlyAsp: 2.944 ± 0.063
4.217GlyGlu: 4.217 ± 0.077
3.116GlyPhe: 3.116 ± 0.068
4.311GlyGly: 4.311 ± 0.101
1.24GlyHis: 1.24 ± 0.037
5.719GlyIle: 5.719 ± 0.082
4.966GlyLys: 4.966 ± 0.078
6.3GlyLeu: 6.3 ± 0.091
2.095GlyMet: 2.095 ± 0.049
2.996GlyAsn: 2.996 ± 0.067
1.317GlyPro: 1.317 ± 0.046
2.414GlyGln: 2.414 ± 0.054
2.185GlyArg: 2.185 ± 0.059
3.629GlySer: 3.629 ± 0.071
4.087GlyThr: 4.087 ± 0.085
4.456GlyVal: 4.456 ± 0.087
0.669GlyTrp: 0.669 ± 0.036
2.839GlyTyr: 2.839 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.175HisAla: 1.175 ± 0.037
0.171HisCys: 0.171 ± 0.016
0.928HisAsp: 0.928 ± 0.033
1.286HisGlu: 1.286 ± 0.047
1.152HisPhe: 1.152 ± 0.037
1.168HisGly: 1.168 ± 0.039
0.662HisHis: 0.662 ± 0.031
1.377HisIle: 1.377 ± 0.044
0.851HisLys: 0.851 ± 0.032
2.244HisLeu: 2.244 ± 0.053
0.415HisMet: 0.415 ± 0.023
0.6HisAsn: 0.6 ± 0.023
0.928HisPro: 0.928 ± 0.033
1.169HisGln: 1.169 ± 0.042
0.688HisArg: 0.688 ± 0.03
1.015HisSer: 1.015 ± 0.039
0.982HisThr: 0.982 ± 0.039
1.25HisVal: 1.25 ± 0.039
0.184HisTrp: 0.184 ± 0.015
1.042HisTyr: 1.042 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.359IleAla: 6.359 ± 0.101
0.678IleCys: 0.678 ± 0.029
4.615IleAsp: 4.615 ± 0.085
5.998IleGlu: 5.998 ± 0.104
3.397IlePhe: 3.397 ± 0.064
5.721IleGly: 5.721 ± 0.1
1.67IleHis: 1.67 ± 0.046
5.335IleIle: 5.335 ± 0.105
4.858IleLys: 4.858 ± 0.083
7.438IleLeu: 7.438 ± 0.111
1.692IleMet: 1.692 ± 0.053
2.924IleAsn: 2.924 ± 0.064
3.26IlePro: 3.26 ± 0.07
3.67IleGln: 3.67 ± 0.071
2.654IleArg: 2.654 ± 0.065
4.936IleSer: 4.936 ± 0.082
4.577IleThr: 4.577 ± 0.08
5.492IleVal: 5.492 ± 0.089
0.581IleTrp: 0.581 ± 0.029
2.774IleTyr: 2.774 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
4.729LysAla: 4.729 ± 0.085
0.279LysCys: 0.279 ± 0.019
3.798LysAsp: 3.798 ± 0.078
7.334LysGlu: 7.334 ± 0.117
1.983LysPhe: 1.983 ± 0.047
4.023LysGly: 4.023 ± 0.082
1.119LysHis: 1.119 ± 0.04
5.447LysIle: 5.447 ± 0.097
6.956LysLys: 6.956 ± 0.111
5.745LysLeu: 5.745 ± 0.094
2.668LysMet: 2.668 ± 0.056
4.198LysAsn: 4.198 ± 0.079
1.991LysPro: 1.991 ± 0.049
3.358LysGln: 3.358 ± 0.062
2.752LysArg: 2.752 ± 0.06
3.22LysSer: 3.22 ± 0.065
3.783LysThr: 3.783 ± 0.072
4.508LysVal: 4.508 ± 0.078
0.694LysTrp: 0.694 ± 0.034
2.343LysTyr: 2.343 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
8.278LeuAla: 8.278 ± 0.11
0.579LeuCys: 0.579 ± 0.031
4.902LeuAsp: 4.902 ± 0.074
6.428LeuGlu: 6.428 ± 0.104
5.061LeuPhe: 5.061 ± 0.113
6.18LeuGly: 6.18 ± 0.109
1.65LeuHis: 1.65 ± 0.046
7.318LeuIle: 7.318 ± 0.132
6.925LeuLys: 6.925 ± 0.089
10.544LeuLeu: 10.544 ± 0.187
2.748LeuMet: 2.748 ± 0.057
4.677LeuAsn: 4.677 ± 0.08
3.966LeuPro: 3.966 ± 0.079
3.525LeuGln: 3.525 ± 0.078
2.995LeuArg: 2.995 ± 0.064
6.967LeuSer: 6.967 ± 0.113
6.854LeuThr: 6.854 ± 0.107
6.416LeuVal: 6.416 ± 0.09
0.706LeuTrp: 0.706 ± 0.031
3.058LeuTyr: 3.058 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.0MetAla: 2.0 ± 0.055
0.136MetCys: 0.136 ± 0.015
1.422MetAsp: 1.422 ± 0.043
1.714MetGlu: 1.714 ± 0.051
0.96MetPhe: 0.96 ± 0.037
1.703MetGly: 1.703 ± 0.045
0.44MetHis: 0.44 ± 0.022
2.474MetIle: 2.474 ± 0.062
2.577MetLys: 2.577 ± 0.061
2.752MetLeu: 2.752 ± 0.062
1.084MetMet: 1.084 ± 0.046
1.604MetAsn: 1.604 ± 0.049
1.062MetPro: 1.062 ± 0.037
1.033MetGln: 1.033 ± 0.037
1.021MetArg: 1.021 ± 0.036
1.893MetSer: 1.893 ± 0.049
1.974MetThr: 1.974 ± 0.052
1.774MetVal: 1.774 ± 0.053
0.133MetTrp: 0.133 ± 0.014
0.702MetTyr: 0.702 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
2.77AsnAla: 2.77 ± 0.064
0.279AsnCys: 0.279 ± 0.019
2.29AsnAsp: 2.29 ± 0.053
3.599AsnGlu: 3.599 ± 0.068
1.89AsnPhe: 1.89 ± 0.054
3.308AsnGly: 3.308 ± 0.066
1.124AsnHis: 1.124 ± 0.034
3.347AsnIle: 3.347 ± 0.073
3.02AsnLys: 3.02 ± 0.066
4.174AsnLeu: 4.174 ± 0.083
1.186AsnMet: 1.186 ± 0.036
2.038AsnAsn: 2.038 ± 0.063
2.035AsnPro: 2.035 ± 0.05
2.987AsnGln: 2.987 ± 0.064
1.802AsnArg: 1.802 ± 0.046
2.056AsnSer: 2.056 ± 0.052
2.187AsnThr: 2.187 ± 0.064
2.78AsnVal: 2.78 ± 0.064
0.502AsnTrp: 0.502 ± 0.029
1.641AsnTyr: 1.641 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
2.548ProAla: 2.548 ± 0.068
0.148ProCys: 0.148 ± 0.014
1.809ProAsp: 1.809 ± 0.052
2.773ProGlu: 2.773 ± 0.056
1.725ProPhe: 1.725 ± 0.047
1.853ProGly: 1.853 ± 0.057
0.727ProHis: 0.727 ± 0.03
3.023ProIle: 3.023 ± 0.07
2.202ProLys: 2.202 ± 0.047
3.209ProLeu: 3.209 ± 0.069
0.853ProMet: 0.853 ± 0.031
1.686ProAsn: 1.686 ± 0.051
0.779ProPro: 0.779 ± 0.039
1.242ProGln: 1.242 ± 0.041
0.794ProArg: 0.794 ± 0.034
2.033ProSer: 2.033 ± 0.053
2.365ProThr: 2.365 ± 0.067
2.52ProVal: 2.52 ± 0.063
0.258ProTrp: 0.258 ± 0.017
1.325ProTyr: 1.325 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
3.45GlnAla: 3.45 ± 0.062
0.18GlnCys: 0.18 ± 0.014
1.863GlnAsp: 1.863 ± 0.05
3.263GlnGlu: 3.263 ± 0.069
1.881GlnPhe: 1.881 ± 0.052
2.315GlnGly: 2.315 ± 0.06
0.921GlnHis: 0.921 ± 0.037
3.106GlnIle: 3.106 ± 0.074
3.349GlnLys: 3.349 ± 0.072
5.135GlnLeu: 5.135 ± 0.083
1.297GlnMet: 1.297 ± 0.04
1.948GlnAsn: 1.948 ± 0.056
1.439GlnPro: 1.439 ± 0.036
2.588GlnGln: 2.588 ± 0.075
1.347GlnArg: 1.347 ± 0.044
2.143GlnSer: 2.143 ± 0.052
2.642GlnThr: 2.642 ± 0.058
3.193GlnVal: 3.193 ± 0.062
0.332GlnTrp: 0.332 ± 0.022
1.619GlnTyr: 1.619 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.096ArgAla: 2.096 ± 0.058
0.171ArgCys: 0.171 ± 0.015
1.549ArgAsp: 1.549 ± 0.049
2.474ArgGlu: 2.474 ± 0.063
1.567ArgPhe: 1.567 ± 0.052
1.937ArgGly: 1.937 ± 0.062
0.649ArgHis: 0.649 ± 0.032
2.773ArgIle: 2.773 ± 0.066
2.811ArgLys: 2.811 ± 0.064
3.365ArgLeu: 3.365 ± 0.071
1.116ArgMet: 1.116 ± 0.038
1.553ArgAsn: 1.553 ± 0.041
1.013ArgPro: 1.013 ± 0.041
1.347ArgGln: 1.347 ± 0.036
1.385ArgArg: 1.385 ± 0.045
1.692ArgSer: 1.692 ± 0.046
1.777ArgThr: 1.777 ± 0.045
2.232ArgVal: 2.232 ± 0.057
0.226ArgTrp: 0.226 ± 0.017
1.429ArgTyr: 1.429 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.728SerAla: 3.728 ± 0.073
0.36SerCys: 0.36 ± 0.026
2.934SerAsp: 2.934 ± 0.068
3.979SerGlu: 3.979 ± 0.078
3.186SerPhe: 3.186 ± 0.071
4.129SerGly: 4.129 ± 0.084
1.112SerHis: 1.112 ± 0.038
4.769SerIle: 4.769 ± 0.086
3.792SerLys: 3.792 ± 0.075
5.899SerLeu: 5.899 ± 0.095
1.625SerMet: 1.625 ± 0.047
2.523SerAsn: 2.523 ± 0.06
1.769SerPro: 1.769 ± 0.05
2.31SerGln: 2.31 ± 0.059
1.876SerArg: 1.876 ± 0.05
3.48SerSer: 3.48 ± 0.08
3.125SerThr: 3.125 ± 0.077
3.783SerVal: 3.783 ± 0.071
0.573SerTrp: 0.573 ± 0.026
2.504SerTyr: 2.504 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
4.605ThrAla: 4.605 ± 0.079
0.324ThrCys: 0.324 ± 0.025
3.181ThrAsp: 3.181 ± 0.076
4.029ThrGlu: 4.029 ± 0.08
2.968ThrPhe: 2.968 ± 0.074
4.393ThrGly: 4.393 ± 0.09
1.085ThrHis: 1.085 ± 0.039
5.349ThrIle: 5.349 ± 0.091
3.812ThrLys: 3.812 ± 0.063
6.036ThrLeu: 6.036 ± 0.088
1.418ThrMet: 1.418 ± 0.048
2.863ThrAsn: 2.863 ± 0.065
2.43ThrPro: 2.43 ± 0.068
1.975ThrGln: 1.975 ± 0.047
1.785ThrArg: 1.785 ± 0.05
3.417ThrSer: 3.417 ± 0.074
3.979ThrThr: 3.979 ± 0.095
4.366ThrVal: 4.366 ± 0.112
0.443ThrTrp: 0.443 ± 0.026
2.315ThrTyr: 2.315 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
5.7ValAla: 5.7 ± 0.085
0.502ValCys: 0.502 ± 0.025
3.588ValAsp: 3.588 ± 0.082
4.561ValGlu: 4.561 ± 0.088
2.869ValPhe: 2.869 ± 0.054
4.449ValGly: 4.449 ± 0.079
1.12ValHis: 1.12 ± 0.037
5.371ValIle: 5.371 ± 0.09
4.368ValLys: 4.368 ± 0.08
6.794ValLeu: 6.794 ± 0.115
1.845ValMet: 1.845 ± 0.046
2.801ValAsn: 2.801 ± 0.066
2.591ValPro: 2.591 ± 0.053
2.277ValGln: 2.277 ± 0.048
2.105ValArg: 2.105 ± 0.059
4.417ValSer: 4.417 ± 0.072
4.777ValThr: 4.777 ± 0.088
5.056ValVal: 5.056 ± 0.096
0.458ValTrp: 0.458 ± 0.024
2.201ValTyr: 2.201 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
0.48TrpAla: 0.48 ± 0.027
0.075TrpCys: 0.075 ± 0.01
0.472TrpAsp: 0.472 ± 0.028
0.498TrpGlu: 0.498 ± 0.026
0.415TrpPhe: 0.415 ± 0.025
0.573TrpGly: 0.573 ± 0.028
0.219TrpHis: 0.219 ± 0.015
0.618TrpIle: 0.618 ± 0.032
0.506TrpLys: 0.506 ± 0.024
1.057TrpLeu: 1.057 ± 0.038
0.242TrpMet: 0.242 ± 0.017
0.407TrpAsn: 0.407 ± 0.026
0.188TrpPro: 0.188 ± 0.017
0.522TrpGln: 0.522 ± 0.029
0.291TrpArg: 0.291 ± 0.023
0.496TrpSer: 0.496 ± 0.028
0.453TrpThr: 0.453 ± 0.025
0.515TrpVal: 0.515 ± 0.027
0.104TrpTrp: 0.104 ± 0.014
0.327TrpTyr: 0.327 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.245TyrAla: 2.245 ± 0.053
0.301TyrCys: 0.301 ± 0.02
2.078TyrAsp: 2.078 ± 0.055
2.692TyrGlu: 2.692 ± 0.063
2.001TyrPhe: 2.001 ± 0.055
2.512TyrGly: 2.512 ± 0.056
0.959TyrHis: 0.959 ± 0.036
2.254TyrIle: 2.254 ± 0.047
1.636TyrLys: 1.636 ± 0.045
4.454TyrLeu: 4.454 ± 0.077
0.781TyrMet: 0.781 ± 0.034
1.256TyrAsn: 1.256 ± 0.041
1.452TyrPro: 1.452 ± 0.045
2.951TyrGln: 2.951 ± 0.08
1.532TyrArg: 1.532 ± 0.044
1.992TyrSer: 1.992 ± 0.052
2.122TyrThr: 2.122 ± 0.061
2.145TyrVal: 2.145 ± 0.054
0.346TyrTrp: 0.346 ± 0.021
1.609TyrTyr: 1.609 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2700 proteins (818632 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski