Mycobacterium phage Phlei

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9BDN6|A0A0N9BDN6_9CAUD Uncharacterized protein OS=Mycobacterium phage Phlei OX=1690684 PE=4 SV=1
MM1 pKa = 7.32NNIDD5 pKa = 3.49IANIRR10 pKa = 11.84DD11 pKa = 3.68IANHH15 pKa = 6.43IYY17 pKa = 10.26YY18 pKa = 9.54EE19 pKa = 4.07VWEE22 pKa = 4.2AEE24 pKa = 3.81RR25 pKa = 11.84PVFAEE30 pKa = 4.17QIAARR35 pKa = 11.84PVALEE40 pKa = 3.31TDD42 pKa = 3.78YY43 pKa = 11.48AIRR46 pKa = 11.84AGDD49 pKa = 3.69ALLFIGPDD57 pKa = 3.17QQYY60 pKa = 10.87PDD62 pKa = 5.0GICWAIYY69 pKa = 9.62TSIKK73 pKa = 10.52DD74 pKa = 3.51VEE76 pKa = 4.32LGEE79 pKa = 4.5FYY81 pKa = 11.14SLGGWAIDD89 pKa = 3.76DD90 pKa = 4.12AEE92 pKa = 4.24TAEE95 pKa = 4.24RR96 pKa = 11.84EE97 pKa = 3.78IGEE100 pKa = 4.43IISTLNEE107 pKa = 3.78LSGEE111 pKa = 4.17QQ112 pKa = 3.5

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N6WN50|A0A0N6WN50_9CAUD Uncharacterized protein OS=Mycobacterium phage Phlei OX=1690684 PE=4 SV=1
MM1 pKa = 7.35TVRR4 pKa = 11.84KK5 pKa = 8.75PKK7 pKa = 10.25KK8 pKa = 9.26RR9 pKa = 11.84YY10 pKa = 8.6PPANVTARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84SCVDD24 pKa = 3.19CQAEE28 pKa = 4.74GITTKK33 pKa = 10.51RR34 pKa = 11.84KK35 pKa = 9.77APHH38 pKa = 6.55PGPRR42 pKa = 11.84CTTHH46 pKa = 6.52HH47 pKa = 6.63RR48 pKa = 11.84AKK50 pKa = 10.43KK51 pKa = 8.07AQRR54 pKa = 11.84RR55 pKa = 11.84NSSWAARR62 pKa = 11.84IQVTYY67 pKa = 10.57GITEE71 pKa = 3.77EE72 pKa = 4.74EE73 pKa = 4.25YY74 pKa = 8.74WKK76 pKa = 10.31IYY78 pKa = 9.35EE79 pKa = 4.18FQGGSCYY86 pKa = 9.86ICRR89 pKa = 11.84RR90 pKa = 11.84ATGQKK95 pKa = 9.67KK96 pKa = 9.76RR97 pKa = 11.84LSVDD101 pKa = 3.18HH102 pKa = 6.62DD103 pKa = 3.92HH104 pKa = 6.59KK105 pKa = 9.96TGIVRR110 pKa = 11.84GLLCASCNRR119 pKa = 11.84YY120 pKa = 9.59VLGHH124 pKa = 6.6LRR126 pKa = 11.84DD127 pKa = 4.01DD128 pKa = 4.17PEE130 pKa = 5.18ALEE133 pKa = 4.07RR134 pKa = 11.84AIDD137 pKa = 3.84YY138 pKa = 10.43LEE140 pKa = 4.41WPPAVALLGEE150 pKa = 4.9RR151 pKa = 11.84IAPIEE156 pKa = 4.07AEE158 pKa = 4.25KK159 pKa = 11.34VEE161 pKa = 4.11LRR163 pKa = 11.84AA164 pKa = 4.36

Molecular weight: 18.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15301

42

1100

188.9

20.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.378 ± 0.333

0.85 ± 0.116

7.045 ± 0.203

7.372 ± 0.335

3.379 ± 0.132

7.81 ± 0.378

1.64 ± 0.142

5.438 ± 0.205

4.699 ± 0.222

8.104 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.103

3.64 ± 0.147

5.032 ± 0.255

3.385 ± 0.15

5.875 ± 0.276

5.555 ± 0.219

5.843 ± 0.29

7.32 ± 0.237

2.046 ± 0.122

3.229 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski