Hubei tombus-like virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG15|A0A1L3KG15_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 22 OX=1923269 PE=4 SV=1
MM1 pKa = 7.78EE2 pKa = 4.72GRR4 pKa = 11.84KK5 pKa = 8.87PRR7 pKa = 11.84AGVRR11 pKa = 11.84RR12 pKa = 11.84EE13 pKa = 3.72SDD15 pKa = 3.14GGSWRR20 pKa = 11.84AHH22 pKa = 3.97VHH24 pKa = 5.35SGEE27 pKa = 3.95ASRR30 pKa = 11.84EE31 pKa = 3.97RR32 pKa = 11.84IEE34 pKa = 5.2DD35 pKa = 3.57VEE37 pKa = 5.14DD38 pKa = 4.41EE39 pKa = 4.09IARR42 pKa = 11.84CEE44 pKa = 4.03EE45 pKa = 3.94ILRR48 pKa = 11.84SSRR51 pKa = 11.84SSWRR55 pKa = 11.84QAAPRR60 pKa = 11.84GAADD64 pKa = 3.74SKK66 pKa = 11.15RR67 pKa = 11.84SGQEE71 pKa = 3.33GRR73 pKa = 11.84RR74 pKa = 11.84SGRR77 pKa = 11.84HH78 pKa = 5.04DD79 pKa = 3.18QGASHH84 pKa = 6.6QGPQGSSSPKK94 pKa = 7.85QAARR98 pKa = 11.84KK99 pKa = 9.47GRR101 pKa = 11.84EE102 pKa = 3.93QEE104 pKa = 5.48DD105 pKa = 3.77PVQPQSSGSSSDD117 pKa = 3.66GEE119 pKa = 4.05EE120 pKa = 4.27GQPPEE125 pKa = 4.55VPALAIPTRR134 pKa = 11.84DD135 pKa = 3.51EE136 pKa = 3.94EE137 pKa = 4.54RR138 pKa = 11.84SEE140 pKa = 4.26SGSGSSGEE148 pKa = 4.17EE149 pKa = 4.13TEE151 pKa = 4.75SEE153 pKa = 4.19GDD155 pKa = 3.73EE156 pKa = 4.4EE157 pKa = 4.21PAQAGQAVQEE167 pKa = 4.64DD168 pKa = 3.85ATEE171 pKa = 4.3PVDD174 pKa = 6.12DD175 pKa = 6.34PDD177 pKa = 4.76DD178 pKa = 3.98CWDD181 pKa = 3.52VGGGANAGCINTGLVNFLKK200 pKa = 10.81AKK202 pKa = 10.07LAYY205 pKa = 9.41KK206 pKa = 10.24PRR208 pKa = 11.84FKK210 pKa = 11.15NGVLDD215 pKa = 3.78QALVNYY221 pKa = 9.83AVAQAQIYY229 pKa = 8.76MRR231 pKa = 11.84DD232 pKa = 3.27HH233 pKa = 7.31PARR236 pKa = 11.84VPEE239 pKa = 4.23DD240 pKa = 3.39KK241 pKa = 11.4YY242 pKa = 11.44EE243 pKa = 4.08VFASSIMQAIRR254 pKa = 11.84DD255 pKa = 3.54ISVEE259 pKa = 4.03EE260 pKa = 3.83NNLRR264 pKa = 11.84KK265 pKa = 10.05LEE267 pKa = 4.2LEE269 pKa = 3.99AAALTRR275 pKa = 11.84HH276 pKa = 5.54TNRR279 pKa = 11.84LTVRR283 pKa = 11.84SKK285 pKa = 11.06EE286 pKa = 3.95FVYY289 pKa = 9.51DD290 pKa = 3.78TGAGRR295 pKa = 11.84FSWRR299 pKa = 11.84YY300 pKa = 6.47WFPKK304 pKa = 9.48ASLVRR309 pKa = 11.84GVPTHH314 pKa = 6.79PIEE317 pKa = 4.32EE318 pKa = 4.5EE319 pKa = 4.06EE320 pKa = 4.29IEE322 pKa = 4.18MGGRR326 pKa = 11.84WVDD329 pKa = 2.99IASVFRR335 pKa = 11.84RR336 pKa = 11.84AIRR339 pKa = 11.84QPIVHH344 pKa = 5.23TTIPRR349 pKa = 11.84LLGQEE354 pKa = 3.93MLGFLAA360 pKa = 5.5

Molecular weight: 39.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG15|A0A1L3KG15_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 22 OX=1923269 PE=4 SV=1
MM1 pKa = 7.51FDD3 pKa = 3.05VSRR6 pKa = 11.84ANFSCKK12 pKa = 9.1HH13 pKa = 4.54QRR15 pKa = 11.84EE16 pKa = 4.05MHH18 pKa = 5.73LVEE21 pKa = 4.56DD22 pKa = 3.57HH23 pKa = 6.53VLRR26 pKa = 11.84QEE28 pKa = 3.85RR29 pKa = 11.84FAFHH33 pKa = 6.51NCAPNEE39 pKa = 4.04LDD41 pKa = 3.19SLMRR45 pKa = 11.84RR46 pKa = 11.84HH47 pKa = 6.79VIPQVSPSQEE57 pKa = 3.92YY58 pKa = 10.24IRR60 pKa = 11.84FADD63 pKa = 3.5KK64 pKa = 10.46EE65 pKa = 4.09LRR67 pKa = 11.84ALWRR71 pKa = 11.84SIGAPRR77 pKa = 11.84LSPLDD82 pKa = 4.3DD83 pKa = 4.1EE84 pKa = 5.49SLLSTRR90 pKa = 11.84SRR92 pKa = 11.84HH93 pKa = 4.54VQRR96 pKa = 11.84RR97 pKa = 11.84WKK99 pKa = 10.45RR100 pKa = 11.84IAEE103 pKa = 4.14EE104 pKa = 3.68AATRR108 pKa = 11.84PFSPKK113 pKa = 9.47EE114 pKa = 3.75ALVKK118 pKa = 10.93AFIKK122 pKa = 9.92YY123 pKa = 8.69EE124 pKa = 4.14KK125 pKa = 10.53YY126 pKa = 10.63GLEE129 pKa = 4.0HH130 pKa = 7.71LDD132 pKa = 3.17INKK135 pKa = 9.24PPRR138 pKa = 11.84AIQARR143 pKa = 11.84GPLFTFRR150 pKa = 11.84LAKK153 pKa = 9.6YY154 pKa = 8.92LVPIEE159 pKa = 4.05HH160 pKa = 7.03KK161 pKa = 8.97MWNWKK166 pKa = 9.36PKK168 pKa = 7.74WNHH171 pKa = 4.77GLRR174 pKa = 11.84VFAKK178 pKa = 9.83GRR180 pKa = 11.84NAAEE184 pKa = 4.14RR185 pKa = 11.84AHH187 pKa = 7.13DD188 pKa = 4.44LLKK191 pKa = 10.84LSQVYY196 pKa = 10.34GSATAMIGIDD206 pKa = 3.26HH207 pKa = 6.97SKK209 pKa = 9.92FDD211 pKa = 3.67SRR213 pKa = 11.84VHH215 pKa = 6.43VEE217 pKa = 3.79HH218 pKa = 6.87LKK220 pKa = 10.3ISHH223 pKa = 6.8RR224 pKa = 11.84FNARR228 pKa = 11.84FYY230 pKa = 10.27RR231 pKa = 11.84GQDD234 pKa = 3.1GRR236 pKa = 11.84DD237 pKa = 3.22LSRR240 pKa = 11.84LMRR243 pKa = 11.84CQLKK247 pKa = 10.34NKK249 pKa = 9.72CVTEE253 pKa = 3.75NGIFYY258 pKa = 10.09VVEE261 pKa = 4.11GRR263 pKa = 11.84RR264 pKa = 11.84MSGDD268 pKa = 3.03IDD270 pKa = 3.79TAKK273 pKa = 10.74GNCEE277 pKa = 3.52INLTVLRR284 pKa = 11.84YY285 pKa = 9.28ILRR288 pKa = 11.84GLRR291 pKa = 11.84GAIYY295 pKa = 10.56LDD297 pKa = 4.17GDD299 pKa = 4.04DD300 pKa = 5.08SVVACHH306 pKa = 6.97PDD308 pKa = 3.44DD309 pKa = 3.75VDD311 pKa = 3.56EE312 pKa = 4.26VVRR315 pKa = 11.84RR316 pKa = 11.84VKK318 pKa = 10.7SGAGTGMQSTVEE330 pKa = 4.0VFDD333 pKa = 4.38RR334 pKa = 11.84FSKK337 pKa = 11.36VEE339 pKa = 4.03FCQARR344 pKa = 11.84PIYY347 pKa = 10.49TNGKK351 pKa = 7.18WMMMRR356 pKa = 11.84NPVKK360 pKa = 10.4AISNMCLMMKK370 pKa = 10.37LPEE373 pKa = 4.46EE374 pKa = 4.74GVATRR379 pKa = 11.84LATIGEE385 pKa = 4.66GEE387 pKa = 4.11LHH389 pKa = 6.75ASSGCPTIYY398 pKa = 10.41PFAKK402 pKa = 9.87KK403 pKa = 9.94LRR405 pKa = 11.84RR406 pKa = 11.84HH407 pKa = 5.76GNVDD411 pKa = 3.08EE412 pKa = 5.68AYY414 pKa = 10.27FEE416 pKa = 4.31YY417 pKa = 10.35RR418 pKa = 11.84HH419 pKa = 6.1KK420 pKa = 10.96LNRR423 pKa = 11.84DD424 pKa = 4.73LIPKK428 pKa = 9.73EE429 pKa = 3.96PDD431 pKa = 2.93HH432 pKa = 6.33EE433 pKa = 6.15AEE435 pKa = 4.2ASAWDD440 pKa = 3.77AWDD443 pKa = 3.76LPPYY447 pKa = 10.02YY448 pKa = 10.47NRR450 pKa = 11.84LSVV453 pKa = 3.17

Molecular weight: 52.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1213

360

453

404.3

45.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.574 ± 0.584

1.566 ± 0.306

5.276 ± 0.429

7.749 ± 1.265

3.38 ± 0.505

7.749 ± 1.058

2.721 ± 0.693

4.452 ± 0.122

4.782 ± 0.725

6.843 ± 0.935

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.431

3.545 ± 0.545

5.688 ± 0.408

3.133 ± 0.883

8.574 ± 1.176

7.42 ± 0.812

4.617 ± 1.406

6.678 ± 0.354

1.731 ± 0.027

3.215 ± 0.695
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski