Mesorhizobium amorphae CCNWGS0123

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; Mesorhizobium amorphae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G6YGA2|G6YGA2_9RHIZ Transposase IS116/IS110/IS902 OS=Mesorhizobium amorphae CCNWGS0123 OX=1082933 GN=MEA186_25052 PE=4 SV=1
MM1 pKa = 7.25ATQIAGAGGTTTSFSNTPQAKK22 pKa = 10.03DD23 pKa = 3.35DD24 pKa = 3.8VFNYY28 pKa = 10.35TEE30 pKa = 4.05DD31 pKa = 3.43TVVIVSAAQSIILLDD46 pKa = 3.59VMKK49 pKa = 10.98NDD51 pKa = 4.04LGGNAKK57 pKa = 8.33TLYY60 pKa = 10.62SVDD63 pKa = 4.16DD64 pKa = 5.12GISASTATKK73 pKa = 10.0QYY75 pKa = 11.53APIDD79 pKa = 4.01LTTADD84 pKa = 3.78AQVSGVSAWEE94 pKa = 4.58DD95 pKa = 2.84IGGGVFIRR103 pKa = 11.84INNGKK108 pKa = 9.47VEE110 pKa = 4.34MNLSQYY116 pKa = 11.04LVLHH120 pKa = 6.51GFSSLQALGAGDD132 pKa = 4.74SINEE136 pKa = 4.16TFTYY140 pKa = 10.09AIKK143 pKa = 10.66LGNGTLSWASVSVNIQGSNDD163 pKa = 3.2GATITAAPGADD174 pKa = 3.12KK175 pKa = 10.67TVVEE179 pKa = 4.76AGGVANGTLNDD190 pKa = 4.34PSAQGQLILTDD201 pKa = 3.47IDD203 pKa = 3.55AGQNHH208 pKa = 5.94FQDD211 pKa = 4.19PASLQGSYY219 pKa = 9.34GTFTFDD225 pKa = 2.91TTTGAWTYY233 pKa = 11.72ALNQALADD241 pKa = 4.32PLTQGQAVTDD251 pKa = 3.99TLTVTSADD259 pKa = 3.08GTASYY264 pKa = 10.76NIVVNITGTNDD275 pKa = 3.33AAVLSADD282 pKa = 3.34VRR284 pKa = 11.84NLTEE288 pKa = 4.81GDD290 pKa = 3.35TAADD294 pKa = 3.28ISTSGTLTISDD305 pKa = 3.58VDD307 pKa = 3.9SPEE310 pKa = 3.94TFVAQVGTVGSYY322 pKa = 8.92GTFDD326 pKa = 3.72IDD328 pKa = 4.75SAGAWTYY335 pKa = 8.06TASSAHH341 pKa = 5.94NEE343 pKa = 4.1FVAGQHH349 pKa = 4.94YY350 pKa = 10.96VEE352 pKa = 5.3DD353 pKa = 4.65FDD355 pKa = 5.33VVSADD360 pKa = 3.51GTHH363 pKa = 5.25TTVHH367 pKa = 6.59IDD369 pKa = 2.98ILGG372 pKa = 3.59

Molecular weight: 38.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G6Y9T3|G6Y9T3_9RHIZ DNA-directed RNA polymerase subunit omega OS=Mesorhizobium amorphae CCNWGS0123 OX=1082933 GN=rpoZ PE=3 SV=1
MM1 pKa = 7.67SKK3 pKa = 8.45ITLRR7 pKa = 11.84AAVCRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84GFFLGNAFIFPGAAPANLRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84IIHH39 pKa = 5.57NRR41 pKa = 11.84RR42 pKa = 3.12

Molecular weight: 4.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7083

0

7083

2092589

25

8568

295.4

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.403 ± 0.037

0.832 ± 0.009

5.739 ± 0.025

5.559 ± 0.026

3.892 ± 0.02

8.552 ± 0.038

2.046 ± 0.014

5.429 ± 0.023

3.68 ± 0.025

9.911 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.017

2.701 ± 0.019

5.007 ± 0.023

3.042 ± 0.018

6.821 ± 0.032

5.581 ± 0.025

5.252 ± 0.026

7.474 ± 0.022

1.341 ± 0.014

2.231 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski