Wuhan House Fly Virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alphadrosrhavirus; Hubei alphadrosrhavirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KRN2|A0A0B5KRN2_9RHAB ORF3 OS=Wuhan House Fly Virus 2 OX=1608105 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.45SMCTFQPCVMGAAAHH16 pKa = 6.26VYY18 pKa = 10.03NADD21 pKa = 3.0KK22 pKa = 10.63CAPAEE27 pKa = 4.27SPFGSEE33 pKa = 4.05DD34 pKa = 3.35DD35 pKa = 3.63QAIAEE40 pKa = 4.64GPDD43 pKa = 3.42TLEE46 pKa = 4.4LDD48 pKa = 3.78SVTSYY53 pKa = 11.41NSLMRR58 pKa = 11.84DD59 pKa = 4.34FYY61 pKa = 11.39AGEE64 pKa = 4.08PTTLHH69 pKa = 6.41SEE71 pKa = 4.2LDD73 pKa = 3.51ADD75 pKa = 4.69TVCNMPTISEE85 pKa = 4.48EE86 pKa = 3.91IFGNASSPKK95 pKa = 9.22EE96 pKa = 3.93NVSSSRR102 pKa = 11.84EE103 pKa = 3.6IIAFLDD109 pKa = 3.19SWTSFTEE116 pKa = 3.74INQLKK121 pKa = 9.8EE122 pKa = 3.88SPHH125 pKa = 6.3YY126 pKa = 10.37FFEE129 pKa = 4.75KK130 pKa = 9.26AQIMIKK136 pKa = 10.64APDD139 pKa = 3.66TLKK142 pKa = 10.94EE143 pKa = 3.89IMSGFRR149 pKa = 11.84VLAQVNTALLTEE161 pKa = 4.35FLLRR165 pKa = 11.84IVGSSNPDD173 pKa = 3.83DD174 pKa = 3.7IKK176 pKa = 10.92MFSALARR183 pKa = 11.84GAMYY187 pKa = 10.32ISSGFQKK194 pKa = 10.9LEE196 pKa = 3.91CTKK199 pKa = 10.55SYY201 pKa = 10.92EE202 pKa = 4.16ILSHH206 pKa = 6.85NMEE209 pKa = 5.15LLTHH213 pKa = 6.48HH214 pKa = 6.68VSAVKK219 pKa = 10.82DD220 pKa = 3.77NVTSQSDD227 pKa = 3.58MVEE230 pKa = 4.12SLRR233 pKa = 11.84EE234 pKa = 4.01SVNKK238 pKa = 9.74VVSVTSKK245 pKa = 11.31LEE247 pKa = 4.17AEE249 pKa = 4.62AKK251 pKa = 10.76LLDD254 pKa = 3.5IKK256 pKa = 10.64AAKK259 pKa = 9.6NSPYY263 pKa = 9.75HH264 pKa = 6.6ASSLPSSSSVGTPIQITGYY283 pKa = 10.01KK284 pKa = 8.92CDD286 pKa = 3.55PHH288 pKa = 8.63IIVCNKK294 pKa = 8.75EE295 pKa = 3.8GKK297 pKa = 10.04VDD299 pKa = 3.83VEE301 pKa = 4.33ATLKK305 pKa = 10.86ANKK308 pKa = 9.54DD309 pKa = 3.19LSSIHH314 pKa = 6.94KK315 pKa = 8.68KK316 pKa = 8.31TLDD319 pKa = 3.26VLALFKK325 pKa = 10.43PAALQFLMAVDD336 pKa = 4.26WSTLNSLMPVDD347 pKa = 4.69PEE349 pKa = 4.78LSVKK353 pKa = 10.03QAGEE357 pKa = 3.53MLYY360 pKa = 10.17TKK362 pKa = 10.41FRR364 pKa = 11.84SLYY367 pKa = 7.9GNRR370 pKa = 11.84MPRR373 pKa = 11.84QPEE376 pKa = 4.2TLTLRR381 pKa = 11.84QGKK384 pKa = 8.35NN385 pKa = 2.84

Molecular weight: 42.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KTL0|A0A0B5KTL0_9RHAB Glycoprotein OS=Wuhan House Fly Virus 2 OX=1608105 GN=G PE=4 SV=1
MM1 pKa = 7.73SDD3 pKa = 4.34NIPSHH8 pKa = 5.26SAGRR12 pKa = 11.84IDD14 pKa = 3.83KK15 pKa = 10.3VEE17 pKa = 4.66HH18 pKa = 6.68IISDD22 pKa = 3.61FTEE25 pKa = 4.4QISLILQGTIEE36 pKa = 4.32LSGPEE41 pKa = 4.0CDD43 pKa = 3.77YY44 pKa = 11.33SIFMWRR50 pKa = 11.84VCCAIAYY57 pKa = 8.11DD58 pKa = 3.64VTYY61 pKa = 10.39KK62 pKa = 10.86KK63 pKa = 10.57KK64 pKa = 8.95VTRR67 pKa = 11.84NTQYY71 pKa = 11.38LIASLIYY78 pKa = 10.24QSLRR82 pKa = 11.84CHH84 pKa = 5.88SQLFSSLQPHH94 pKa = 6.57YY95 pKa = 11.04GPLLEE100 pKa = 4.7LVCPIKK106 pKa = 10.91VRR108 pKa = 11.84GYY110 pKa = 8.14TEE112 pKa = 3.97YY113 pKa = 10.35PIEE116 pKa = 4.26TRR118 pKa = 11.84SFPQLNIIRR127 pKa = 11.84EE128 pKa = 3.88RR129 pKa = 11.84SFRR132 pKa = 11.84YY133 pKa = 9.33QVTDD137 pKa = 3.16VKK139 pKa = 10.3QLRR142 pKa = 11.84IRR144 pKa = 11.84SRR146 pKa = 11.84IQLQACQFSDD156 pKa = 3.21NFRR159 pKa = 11.84AEE161 pKa = 4.07ARR163 pKa = 11.84LKK165 pKa = 10.8GYY167 pKa = 10.52VPLLPPSTDD176 pKa = 2.4EE177 pKa = 4.15WIVYY181 pKa = 9.61FSEE184 pKa = 4.55LEE186 pKa = 3.94NAIDD190 pKa = 3.36STGRR194 pKa = 11.84YY195 pKa = 8.88DD196 pKa = 3.97QIDD199 pKa = 3.93VSAHH203 pKa = 4.84IAKK206 pKa = 10.25GIEE209 pKa = 3.89ALTPSKK215 pKa = 10.58PNEE218 pKa = 4.01SVSFVKK224 pKa = 10.5KK225 pKa = 10.75LKK227 pKa = 10.52QFMSKK232 pKa = 9.7SSKK235 pKa = 9.71NAVVEE240 pKa = 4.2ARR242 pKa = 11.84ALAPSHH248 pKa = 7.04PSDD251 pKa = 3.73CLEE254 pKa = 4.78IISSPKK260 pKa = 10.02VVNQQVILSRR270 pKa = 11.84QPKK273 pKa = 9.56NNN275 pKa = 3.31

Molecular weight: 31.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4031

275

2233

806.2

91.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.483 ± 0.677

1.96 ± 0.133

4.986 ± 0.397

5.408 ± 0.252

5.11 ± 0.544

4.838 ± 0.328

2.506 ± 0.082

7.12 ± 0.336

6.773 ± 0.145

10.146 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.233

4.589 ± 0.251

5.036 ± 0.185

3.597 ± 0.407

4.118 ± 0.302

9.799 ± 0.546

5.334 ± 0.247

5.83 ± 0.149

1.439 ± 0.282

3.126 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski