Pelagirhabdus alkalitolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pelagirhabdus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1G6GQV9|A0A1G6GQV9_9BACI Histidine kinase OS=Pelagirhabdus alkalitolerans OX=1612202 GN=SAMN05421734_101408 PE=4 SV=1
MM1 pKa = 6.69MKK3 pKa = 10.17KK4 pKa = 9.77YY5 pKa = 11.0VKK7 pKa = 10.48GFLFAVLITLVLAGCGNGDD26 pKa = 4.12EE27 pKa = 4.92NGNDD31 pKa = 3.29QAGDD35 pKa = 3.83SEE37 pKa = 4.75AEE39 pKa = 4.12AVEE42 pKa = 4.15LTLFSSVTNEE52 pKa = 3.2SDD54 pKa = 3.2QAIFEE59 pKa = 4.27EE60 pKa = 4.73VIEE63 pKa = 4.31RR64 pKa = 11.84FEE66 pKa = 4.25EE67 pKa = 4.37EE68 pKa = 3.99NDD70 pKa = 4.37HH71 pKa = 7.14IQIDD75 pKa = 4.37YY76 pKa = 10.77NFPTSEE82 pKa = 4.43YY83 pKa = 8.69EE84 pKa = 3.75SMMRR88 pKa = 11.84VRR90 pKa = 11.84MGADD94 pKa = 3.33DD95 pKa = 4.34MPDD98 pKa = 3.16LFDD101 pKa = 3.36THH103 pKa = 5.67GWAVNRR109 pKa = 11.84YY110 pKa = 8.83GDD112 pKa = 3.62YY113 pKa = 11.34TMDD116 pKa = 4.17LRR118 pKa = 11.84DD119 pKa = 4.15MDD121 pKa = 4.0WVDD124 pKa = 4.07NLDD127 pKa = 4.0PSLEE131 pKa = 4.34PIITDD136 pKa = 4.06DD137 pKa = 3.95DD138 pKa = 4.69GKK140 pKa = 11.41VYY142 pKa = 10.32AYY144 pKa = 10.06PMNQANDD151 pKa = 3.55GLTYY155 pKa = 10.76NVGILEE161 pKa = 4.74EE162 pKa = 4.16YY163 pKa = 10.63DD164 pKa = 2.97IDD166 pKa = 4.01VPEE169 pKa = 4.59TFDD172 pKa = 3.78EE173 pKa = 4.93LMDD176 pKa = 4.26ALRR179 pKa = 11.84EE180 pKa = 4.09IRR182 pKa = 11.84DD183 pKa = 3.52QSDD186 pKa = 4.08GEE188 pKa = 4.44VTPLWFPGSDD198 pKa = 3.37RR199 pKa = 11.84YY200 pKa = 11.12SLGYY204 pKa = 10.21FLDD207 pKa = 3.68IMSTPLLVTHH217 pKa = 6.97EE218 pKa = 4.1DD219 pKa = 3.06HH220 pKa = 8.04DD221 pKa = 4.58YY222 pKa = 11.54SEE224 pKa = 4.26EE225 pKa = 4.07LKK227 pKa = 10.9AGEE230 pKa = 4.44YY231 pKa = 10.73GWDD234 pKa = 3.41EE235 pKa = 4.0YY236 pKa = 11.42TYY238 pKa = 11.07LPEE241 pKa = 6.2KK242 pKa = 9.57LLQMQEE248 pKa = 3.92EE249 pKa = 4.49DD250 pKa = 4.03LLNVDD255 pKa = 3.77VLTAQTHH262 pKa = 5.11EE263 pKa = 4.18QTQLFAQDD271 pKa = 3.32KK272 pKa = 9.43IGFVMGTLPVDD283 pKa = 4.65SIHH286 pKa = 6.61EE287 pKa = 4.16LNEE290 pKa = 4.74DD291 pKa = 3.44IEE293 pKa = 4.69LGIMPVPPIHH303 pKa = 7.81DD304 pKa = 4.15GGEE307 pKa = 4.01KK308 pKa = 9.95SWIGGEE314 pKa = 4.05RR315 pKa = 11.84FTLAVAQNSDD325 pKa = 3.36HH326 pKa = 7.05PEE328 pKa = 3.63EE329 pKa = 4.26AKK331 pKa = 10.71QFIEE335 pKa = 5.72FLAQPDD341 pKa = 3.54IAQKK345 pKa = 10.35VAEE348 pKa = 4.46GTNLPSGLEE357 pKa = 3.97NVSAEE362 pKa = 3.78IYY364 pKa = 9.87YY365 pKa = 9.84QDD367 pKa = 4.19FYY369 pKa = 11.88EE370 pKa = 5.0EE371 pKa = 4.25YY372 pKa = 10.42DD373 pKa = 3.77DD374 pKa = 6.16VIVEE378 pKa = 4.29PYY380 pKa = 9.85FDD382 pKa = 5.48RR383 pKa = 11.84EE384 pKa = 4.07FLPSGMWDD392 pKa = 3.4VYY394 pKa = 11.55GEE396 pKa = 4.21TAQEE400 pKa = 3.97LLSGRR405 pKa = 11.84MTPEE409 pKa = 3.4EE410 pKa = 4.16VTEE413 pKa = 4.24QMAAEE418 pKa = 4.1YY419 pKa = 10.62NRR421 pKa = 11.84LYY423 pKa = 10.98DD424 pKa = 3.78EE425 pKa = 4.47EE426 pKa = 4.33

Molecular weight:
48.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1G6LCH0|A0A1G6LCH0_9BACI Uncharacterized protein OS=Pelagirhabdus alkalitolerans OX=1612202 GN=SAMN05421734_10835 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 8.86HH12 pKa = 6.39KK13 pKa = 9.52KK14 pKa = 7.68VHH16 pKa = 5.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.35NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.36KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight:
5.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2406

0

2406

744324

31

2683

309.4

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.419 ± 0.048

0.55 ± 0.012

6.432 ± 0.054

7.779 ± 0.066

4.375 ± 0.042

6.273 ± 0.049

2.359 ± 0.029

7.811 ± 0.05

6.088 ± 0.06

9.662 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.025

4.411 ± 0.034

3.397 ± 0.028

4.295 ± 0.032

4.02 ± 0.032

6.155 ± 0.035

5.659 ± 0.034

7.024 ± 0.039

0.888 ± 0.017

3.656 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski