Lake Sarah-associated circular virus-29

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA95|A0A126GA95_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-29 OX=1685756 PE=4 SV=1
MM1 pKa = 7.27SVSDD5 pKa = 4.25DD6 pKa = 3.97FEE8 pKa = 7.01AGAEE12 pKa = 4.13PIQLYY17 pKa = 10.12GALLEE22 pKa = 4.19ICEE25 pKa = 4.12IFAYY29 pKa = 8.25QHH31 pKa = 5.75EE32 pKa = 4.68VAPTTGYY39 pKa = 10.22RR40 pKa = 11.84HH41 pKa = 4.6YY42 pKa = 10.61QGYY45 pKa = 10.34LEE47 pKa = 5.49LVTKK51 pKa = 10.49NRR53 pKa = 11.84HH54 pKa = 3.83TWIQNQLLKK63 pKa = 10.78HH64 pKa = 5.73GLHH67 pKa = 6.47FEE69 pKa = 4.28YY70 pKa = 10.79LVGAKK75 pKa = 9.76GLPKK79 pKa = 10.05QAWAYY84 pKa = 7.26ATKK87 pKa = 10.36EE88 pKa = 4.1DD89 pKa = 4.14TRR91 pKa = 11.84ADD93 pKa = 4.04GPWTFGEE100 pKa = 4.26PRR102 pKa = 11.84DD103 pKa = 3.81SEE105 pKa = 4.36KK106 pKa = 11.27ANKK109 pKa = 9.53SWLFVKK115 pKa = 10.38AIKK118 pKa = 10.52SGATDD123 pKa = 4.67AEE125 pKa = 4.54LCDD128 pKa = 4.31SFPGMMTNSFSNANRR143 pKa = 11.84IRR145 pKa = 11.84QAYY148 pKa = 8.11GVQLPEE154 pKa = 4.19PEE156 pKa = 3.83RR157 pKa = 11.84TVPLEE162 pKa = 3.96LYY164 pKa = 10.48LFYY167 pKa = 11.02GPSGTGKK174 pKa = 9.76SQFARR179 pKa = 11.84DD180 pKa = 3.51QARR183 pKa = 11.84LAKK186 pKa = 9.17MNPYY190 pKa = 9.71VLPIGKK196 pKa = 9.33DD197 pKa = 3.47FWVTPAMCGKK207 pKa = 9.94KK208 pKa = 10.47YY209 pKa = 10.95YY210 pKa = 10.19IIDD213 pKa = 3.98DD214 pKa = 4.26FKK216 pKa = 11.85SNLGLSDD223 pKa = 4.69LLRR226 pKa = 11.84LLDD229 pKa = 4.15TYY231 pKa = 10.9PVEE234 pKa = 4.6APNKK238 pKa = 9.42GIASSEE244 pKa = 3.89FSSLNGGVFNNFVRR258 pKa = 11.84WPYY261 pKa = 10.87VVDD264 pKa = 3.21ARR266 pKa = 11.84NYY268 pKa = 8.35CHH270 pKa = 6.75YY271 pKa = 9.77NQSITSS277 pKa = 3.6

Molecular weight: 31.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA95|A0A126GA95_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-29 OX=1685756 PE=4 SV=1
MM1 pKa = 7.05CTHH4 pKa = 6.87ARR6 pKa = 11.84TWMPCRR12 pKa = 11.84KK13 pKa = 7.65MARR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84VTRR21 pKa = 11.84KK22 pKa = 8.22MKK24 pKa = 10.4KK25 pKa = 8.54PRR27 pKa = 11.84ASARR31 pKa = 11.84RR32 pKa = 11.84NPTQEE37 pKa = 4.59FLMKK41 pKa = 9.83PAIRR45 pKa = 11.84ARR47 pKa = 11.84GSRR50 pKa = 11.84SKK52 pKa = 10.69RR53 pKa = 11.84QGYY56 pKa = 8.34MSAAMGTGLMPCTKK70 pKa = 9.76RR71 pKa = 11.84YY72 pKa = 8.41MDD74 pKa = 5.58SILDD78 pKa = 3.7PSGDD82 pKa = 3.69AGRR85 pKa = 11.84GACVPSGFPIPSQKK99 pKa = 10.31ARR101 pKa = 11.84AFLRR105 pKa = 11.84GTMSTGTGGNGYY117 pKa = 9.57ILFTPSLGNDD127 pKa = 3.05NAIVRR132 pKa = 11.84FTTAANTAVGTEE144 pKa = 3.84ALNNAVYY151 pKa = 8.6TASNSSISAGKK162 pKa = 10.09LPYY165 pKa = 9.32STAQIASGNIDD176 pKa = 3.32ARR178 pKa = 11.84IVSAMIRR185 pKa = 11.84VRR187 pKa = 11.84FAGAEE192 pKa = 4.07DD193 pKa = 3.71NRR195 pKa = 11.84SGIVTLLEE203 pKa = 4.86DD204 pKa = 4.88PDD206 pKa = 4.35HH207 pKa = 7.0NSLFTQTPAQVASFEE222 pKa = 4.26GATRR226 pKa = 11.84EE227 pKa = 4.06RR228 pKa = 11.84PNGDD232 pKa = 3.5GAWSQICWSGPCKK245 pKa = 8.83QTEE248 pKa = 4.11VEE250 pKa = 4.43YY251 pKa = 11.42VNTATQTTQTDD262 pKa = 2.74IMAILINGTTNSAGAPGPQPFEE284 pKa = 3.76YY285 pKa = 10.01EE286 pKa = 3.38AWINCEE292 pKa = 3.94YY293 pKa = 10.34IGRR296 pKa = 11.84DD297 pKa = 3.78AIGKK301 pKa = 8.04TDD303 pKa = 3.54NQNDD307 pKa = 3.87QMGMDD312 pKa = 3.8AVTSIFKK319 pKa = 10.53KK320 pKa = 10.41FAGTPQVQVNPEE332 pKa = 4.03SAPSIRR338 pKa = 11.84QAIRR342 pKa = 11.84THH344 pKa = 5.56QMSVSGSALPRR355 pKa = 11.84PKK357 pKa = 9.86TLAGDD362 pKa = 4.67LIEE365 pKa = 4.66GAVTAFNPGWGRR377 pKa = 11.84LWGSLRR383 pKa = 11.84DD384 pKa = 3.76RR385 pKa = 11.84IRR387 pKa = 11.84ARR389 pKa = 11.84KK390 pKa = 8.65

Molecular weight: 42.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

667

277

390

333.5

36.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.195 ± 1.19

1.649 ± 0.129

4.498 ± 0.35

4.348 ± 0.898

3.898 ± 0.727

8.546 ± 0.607

1.349 ± 0.514

5.097 ± 0.708

4.648 ± 0.71

6.297 ± 1.716

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.999 ± 0.751

5.097 ± 0.027

5.997 ± 0.139

4.198 ± 0.143

6.747 ± 1.519

7.196 ± 0.439

7.196 ± 1.347

4.498 ± 0.35

1.799 ± 0.231

3.748 ± 1.503
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski