Amino acid dipepetide frequency for Escherichia phage slur16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.811AlaAla: 5.811 ± 0.47
1.335AlaCys: 1.335 ± 0.186
4.328AlaAsp: 4.328 ± 0.331
4.872AlaGlu: 4.872 ± 0.456
2.77AlaPhe: 2.77 ± 0.245
4.995AlaGly: 4.995 ± 0.492
1.014AlaHis: 1.014 ± 0.127
3.833AlaIle: 3.833 ± 0.323
5.564AlaLys: 5.564 ± 0.441
5.267AlaLeu: 5.267 ± 0.425
2.374AlaMet: 2.374 ± 0.259
3.783AlaAsn: 3.783 ± 0.355
2.102AlaPro: 2.102 ± 0.262
2.077AlaGln: 2.077 ± 0.247
3.017AlaArg: 3.017 ± 0.229
3.437AlaSer: 3.437 ± 0.31
4.031AlaThr: 4.031 ± 0.48
4.575AlaVal: 4.575 ± 0.355
1.286AlaTrp: 1.286 ± 0.166
3.239AlaTyr: 3.239 ± 0.292
0.0AlaXaa: 0.0 ± 0.0
Cys
0.964CysAla: 0.964 ± 0.165
0.42CysCys: 0.42 ± 0.117
0.866CysAsp: 0.866 ± 0.157
1.063CysGlu: 1.063 ± 0.162
0.519CysPhe: 0.519 ± 0.108
1.41CysGly: 1.41 ± 0.175
0.445CysHis: 0.445 ± 0.112
0.791CysIle: 0.791 ± 0.176
0.989CysLys: 0.989 ± 0.177
1.162CysLeu: 1.162 ± 0.191
0.495CysMet: 0.495 ± 0.114
0.841CysAsn: 0.841 ± 0.137
0.767CysPro: 0.767 ± 0.14
0.445CysGln: 0.445 ± 0.106
0.989CysArg: 0.989 ± 0.16
0.816CysSer: 0.816 ± 0.128
0.742CysThr: 0.742 ± 0.158
1.286CysVal: 1.286 ± 0.156
0.371CysTrp: 0.371 ± 0.082
0.717CysTyr: 0.717 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
4.229AspAla: 4.229 ± 0.3
1.187AspCys: 1.187 ± 0.177
4.179AspAsp: 4.179 ± 0.379
4.402AspGlu: 4.402 ± 0.375
3.338AspPhe: 3.338 ± 0.302
5.218AspGly: 5.218 ± 0.389
1.533AspHis: 1.533 ± 0.177
4.08AspIle: 4.08 ± 0.329
4.797AspLys: 4.797 ± 0.401
6.009AspLeu: 6.009 ± 0.351
1.978AspMet: 1.978 ± 0.29
3.61AspAsn: 3.61 ± 0.339
3.536AspPro: 3.536 ± 0.31
1.83AspGln: 1.83 ± 0.186
2.967AspArg: 2.967 ± 0.262
3.511AspSer: 3.511 ± 0.32
2.992AspThr: 2.992 ± 0.304
4.723AspVal: 4.723 ± 0.343
1.212AspTrp: 1.212 ± 0.167
2.794AspTyr: 2.794 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
5.119GluAla: 5.119 ± 0.336
0.767GluCys: 0.767 ± 0.145
4.822GluAsp: 4.822 ± 0.38
6.85GluGlu: 6.85 ± 0.646
2.943GluPhe: 2.943 ± 0.235
5.44GluGly: 5.44 ± 0.401
1.212GluHis: 1.212 ± 0.192
4.451GluIle: 4.451 ± 0.379
4.352GluLys: 4.352 ± 0.415
6.504GluLeu: 6.504 ± 0.486
2.275GluMet: 2.275 ± 0.27
3.462GluAsn: 3.462 ± 0.314
2.003GluPro: 2.003 ± 0.212
1.954GluGln: 1.954 ± 0.232
2.992GluArg: 2.992 ± 0.237
3.363GluSer: 3.363 ± 0.317
2.893GluThr: 2.893 ± 0.285
5.514GluVal: 5.514 ± 0.351
1.286GluTrp: 1.286 ± 0.18
2.967GluTyr: 2.967 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.473PheAla: 2.473 ± 0.253
0.519PheCys: 0.519 ± 0.115
3.215PheAsp: 3.215 ± 0.321
3.19PheGlu: 3.19 ± 0.323
1.484PhePhe: 1.484 ± 0.195
3.116PheGly: 3.116 ± 0.306
0.841PheHis: 0.841 ± 0.132
2.597PheIle: 2.597 ± 0.308
2.893PheLys: 2.893 ± 0.229
3.165PheLeu: 3.165 ± 0.282
1.36PheMet: 1.36 ± 0.184
2.374PheAsn: 2.374 ± 0.257
1.41PhePro: 1.41 ± 0.2
1.138PheGln: 1.138 ± 0.2
1.879PheArg: 1.879 ± 0.192
2.695PheSer: 2.695 ± 0.301
2.201PheThr: 2.201 ± 0.209
2.893PheVal: 2.893 ± 0.235
0.668PheTrp: 0.668 ± 0.107
2.201PheTyr: 2.201 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
4.55GlyAla: 4.55 ± 0.439
1.063GlyCys: 1.063 ± 0.177
5.069GlyAsp: 5.069 ± 0.351
4.97GlyGlu: 4.97 ± 0.292
3.413GlyPhe: 3.413 ± 0.329
5.218GlyGly: 5.218 ± 0.561
1.36GlyHis: 1.36 ± 0.2
3.957GlyIle: 3.957 ± 0.288
5.564GlyLys: 5.564 ± 0.378
4.946GlyLeu: 4.946 ± 0.34
1.904GlyMet: 1.904 ± 0.198
3.882GlyAsn: 3.882 ± 0.3
1.41GlyPro: 1.41 ± 0.256
1.954GlyGln: 1.954 ± 0.211
3.363GlyArg: 3.363 ± 0.279
3.536GlySer: 3.536 ± 0.294
4.402GlyThr: 4.402 ± 0.711
4.847GlyVal: 4.847 ± 0.346
1.335GlyTrp: 1.335 ± 0.174
3.808GlyTyr: 3.808 ± 0.322
0.0GlyXaa: 0.0 ± 0.0
His
0.94HisAla: 0.94 ± 0.169
0.445HisCys: 0.445 ± 0.098
1.063HisAsp: 1.063 ± 0.192
1.014HisGlu: 1.014 ± 0.165
0.915HisPhe: 0.915 ± 0.135
1.039HisGly: 1.039 ± 0.142
0.371HisHis: 0.371 ± 0.104
0.989HisIle: 0.989 ± 0.171
1.484HisLys: 1.484 ± 0.194
1.756HisLeu: 1.756 ± 0.195
0.47HisMet: 0.47 ± 0.109
0.989HisAsn: 0.989 ± 0.153
1.286HisPro: 1.286 ± 0.226
0.593HisGln: 0.593 ± 0.122
0.964HisArg: 0.964 ± 0.162
1.113HisSer: 1.113 ± 0.188
1.459HisThr: 1.459 ± 0.183
1.063HisVal: 1.063 ± 0.186
0.321HisTrp: 0.321 ± 0.098
1.039HisTyr: 1.039 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.08IleAla: 4.08 ± 0.314
0.94IleCys: 0.94 ± 0.165
4.006IleAsp: 4.006 ± 0.354
3.586IleGlu: 3.586 ± 0.256
2.102IlePhe: 2.102 ± 0.26
3.635IleGly: 3.635 ± 0.298
1.261IleHis: 1.261 ± 0.171
3.808IleIle: 3.808 ± 0.332
3.61IleLys: 3.61 ± 0.358
4.723IleLeu: 4.723 ± 0.407
1.682IleMet: 1.682 ± 0.185
3.091IleAsn: 3.091 ± 0.289
2.745IlePro: 2.745 ± 0.26
2.028IleGln: 2.028 ± 0.229
2.967IleArg: 2.967 ± 0.263
3.462IleSer: 3.462 ± 0.348
3.66IleThr: 3.66 ± 0.3
4.377IleVal: 4.377 ± 0.334
0.692IleTrp: 0.692 ± 0.121
2.226IleTyr: 2.226 ± 0.25
0.0IleXaa: 0.0 ± 0.0
Lys
5.712LysAla: 5.712 ± 0.396
1.236LysCys: 1.236 ± 0.169
5.045LysAsp: 5.045 ± 0.425
5.391LysGlu: 5.391 ± 0.485
2.72LysPhe: 2.72 ± 0.27
4.55LysGly: 4.55 ± 0.355
1.311LysHis: 1.311 ± 0.205
4.575LysIle: 4.575 ± 0.354
4.723LysLys: 4.723 ± 0.378
4.822LysLeu: 4.822 ± 0.338
2.226LysMet: 2.226 ± 0.244
2.893LysAsn: 2.893 ± 0.246
2.374LysPro: 2.374 ± 0.259
2.3LysGln: 2.3 ± 0.193
2.77LysArg: 2.77 ± 0.246
3.462LysSer: 3.462 ± 0.322
3.957LysThr: 3.957 ± 0.281
5.094LysVal: 5.094 ± 0.408
1.014LysTrp: 1.014 ± 0.144
2.77LysTyr: 2.77 ± 0.254
0.0LysXaa: 0.0 ± 0.0
Leu
5.589LeuAla: 5.589 ± 0.33
0.989LeuCys: 0.989 ± 0.181
6.059LeuAsp: 6.059 ± 0.409
6.429LeuGlu: 6.429 ± 0.541
2.597LeuPhe: 2.597 ± 0.274
4.55LeuGly: 4.55 ± 0.364
1.731LeuHis: 1.731 ± 0.219
4.229LeuIle: 4.229 ± 0.355
5.119LeuLys: 5.119 ± 0.377
5.02LeuLeu: 5.02 ± 0.425
2.127LeuMet: 2.127 ± 0.24
4.426LeuAsn: 4.426 ± 0.323
3.932LeuPro: 3.932 ± 0.242
2.671LeuGln: 2.671 ± 0.28
3.487LeuArg: 3.487 ± 0.327
4.426LeuSer: 4.426 ± 0.294
4.476LeuThr: 4.476 ± 0.365
5.119LeuVal: 5.119 ± 0.315
1.434LeuTrp: 1.434 ± 0.18
2.819LeuTyr: 2.819 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
2.473MetAla: 2.473 ± 0.261
0.42MetCys: 0.42 ± 0.109
1.335MetAsp: 1.335 ± 0.179
1.731MetGlu: 1.731 ± 0.202
1.113MetPhe: 1.113 ± 0.183
1.756MetGly: 1.756 ± 0.218
0.47MetHis: 0.47 ± 0.115
1.879MetIle: 1.879 ± 0.211
2.967MetLys: 2.967 ± 0.258
2.522MetLeu: 2.522 ± 0.219
0.816MetMet: 0.816 ± 0.147
1.063MetAsn: 1.063 ± 0.174
0.989MetPro: 0.989 ± 0.151
1.113MetGln: 1.113 ± 0.165
1.212MetArg: 1.212 ± 0.156
2.052MetSer: 2.052 ± 0.189
1.583MetThr: 1.583 ± 0.194
1.41MetVal: 1.41 ± 0.171
0.643MetTrp: 0.643 ± 0.126
0.767MetTyr: 0.767 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
3.808AsnAla: 3.808 ± 0.3
0.767AsnCys: 0.767 ± 0.132
2.992AsnAsp: 2.992 ± 0.241
2.597AsnGlu: 2.597 ± 0.229
2.448AsnPhe: 2.448 ± 0.269
4.575AsnGly: 4.575 ± 0.409
0.915AsnHis: 0.915 ± 0.152
3.066AsnIle: 3.066 ± 0.274
3.487AsnLys: 3.487 ± 0.293
4.105AsnLeu: 4.105 ± 0.306
1.236AsnMet: 1.236 ± 0.154
2.918AsnAsn: 2.918 ± 0.332
2.275AsnPro: 2.275 ± 0.223
1.607AsnGln: 1.607 ± 0.183
2.25AsnArg: 2.25 ± 0.2
2.646AsnSer: 2.646 ± 0.274
3.116AsnThr: 3.116 ± 0.3
2.918AsnVal: 2.918 ± 0.257
0.915AsnTrp: 0.915 ± 0.146
1.632AsnTyr: 1.632 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
2.151ProAla: 2.151 ± 0.266
0.593ProCys: 0.593 ± 0.114
3.338ProAsp: 3.338 ± 0.297
3.833ProGlu: 3.833 ± 0.29
2.028ProPhe: 2.028 ± 0.21
2.498ProGly: 2.498 ± 0.295
0.692ProHis: 0.692 ± 0.134
1.607ProIle: 1.607 ± 0.201
2.374ProLys: 2.374 ± 0.306
2.819ProLeu: 2.819 ± 0.232
0.866ProMet: 0.866 ± 0.147
1.607ProAsn: 1.607 ± 0.234
1.162ProPro: 1.162 ± 0.217
1.385ProGln: 1.385 ± 0.198
1.558ProArg: 1.558 ± 0.23
2.695ProSer: 2.695 ± 0.237
2.077ProThr: 2.077 ± 0.217
3.066ProVal: 3.066 ± 0.285
0.371ProTrp: 0.371 ± 0.121
1.385ProTyr: 1.385 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
2.695GlnAla: 2.695 ± 0.265
0.346GlnCys: 0.346 ± 0.107
1.805GlnAsp: 1.805 ± 0.214
2.547GlnGlu: 2.547 ± 0.276
1.36GlnPhe: 1.36 ± 0.185
1.682GlnGly: 1.682 ± 0.194
0.495GlnHis: 0.495 ± 0.109
1.855GlnIle: 1.855 ± 0.216
1.929GlnLys: 1.929 ± 0.251
2.77GlnLeu: 2.77 ± 0.211
1.063GlnMet: 1.063 ± 0.169
1.212GlnAsn: 1.212 ± 0.181
1.385GlnPro: 1.385 ± 0.198
1.78GlnGln: 1.78 ± 0.266
1.558GlnArg: 1.558 ± 0.153
1.484GlnSer: 1.484 ± 0.208
1.682GlnThr: 1.682 ± 0.212
2.3GlnVal: 2.3 ± 0.332
0.767GlnTrp: 0.767 ± 0.132
1.335GlnTyr: 1.335 ± 0.221
0.0GlnXaa: 0.0 ± 0.0
Arg
2.498ArgAla: 2.498 ± 0.237
0.989ArgCys: 0.989 ± 0.145
2.918ArgAsp: 2.918 ± 0.253
2.695ArgGlu: 2.695 ± 0.31
1.731ArgPhe: 1.731 ± 0.21
2.819ArgGly: 2.819 ± 0.298
0.668ArgHis: 0.668 ± 0.125
2.72ArgIle: 2.72 ± 0.19
3.462ArgLys: 3.462 ± 0.26
3.882ArgLeu: 3.882 ± 0.28
1.632ArgMet: 1.632 ± 0.229
2.25ArgAsn: 2.25 ± 0.263
1.311ArgPro: 1.311 ± 0.196
1.508ArgGln: 1.508 ± 0.182
2.671ArgArg: 2.671 ± 0.245
2.572ArgSer: 2.572 ± 0.224
2.572ArgThr: 2.572 ± 0.318
3.487ArgVal: 3.487 ± 0.279
0.841ArgTrp: 0.841 ± 0.143
2.052ArgTyr: 2.052 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
3.907SerAla: 3.907 ± 0.339
1.039SerCys: 1.039 ± 0.157
3.61SerAsp: 3.61 ± 0.268
3.314SerGlu: 3.314 ± 0.293
2.498SerPhe: 2.498 ± 0.241
4.97SerGly: 4.97 ± 0.42
1.162SerHis: 1.162 ± 0.173
3.091SerIle: 3.091 ± 0.266
3.833SerLys: 3.833 ± 0.372
3.437SerLeu: 3.437 ± 0.301
1.533SerMet: 1.533 ± 0.166
2.77SerAsn: 2.77 ± 0.266
2.3SerPro: 2.3 ± 0.224
1.706SerGln: 1.706 ± 0.231
2.448SerArg: 2.448 ± 0.23
3.19SerSer: 3.19 ± 0.313
3.388SerThr: 3.388 ± 0.277
4.031SerVal: 4.031 ± 0.343
0.866SerTrp: 0.866 ± 0.158
2.127SerTyr: 2.127 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
4.031ThrAla: 4.031 ± 0.384
0.915ThrCys: 0.915 ± 0.157
3.165ThrAsp: 3.165 ± 0.321
3.66ThrGlu: 3.66 ± 0.327
2.819ThrPhe: 2.819 ± 0.267
4.328ThrGly: 4.328 ± 0.344
1.261ThrHis: 1.261 ± 0.185
3.981ThrIle: 3.981 ± 0.307
3.338ThrLys: 3.338 ± 0.33
4.698ThrLeu: 4.698 ± 0.41
1.113ThrMet: 1.113 ± 0.156
2.448ThrAsn: 2.448 ± 0.322
2.473ThrPro: 2.473 ± 0.277
1.83ThrGln: 1.83 ± 0.215
2.127ThrArg: 2.127 ± 0.236
3.462ThrSer: 3.462 ± 0.416
3.437ThrThr: 3.437 ± 0.414
4.575ThrVal: 4.575 ± 0.402
0.989ThrTrp: 0.989 ± 0.149
2.25ThrTyr: 2.25 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
4.748ValAla: 4.748 ± 0.403
1.088ValCys: 1.088 ± 0.168
6.009ValAsp: 6.009 ± 0.407
5.292ValGlu: 5.292 ± 0.423
3.314ValPhe: 3.314 ± 0.276
4.649ValGly: 4.649 ± 0.309
1.286ValHis: 1.286 ± 0.217
4.154ValIle: 4.154 ± 0.301
4.822ValLys: 4.822 ± 0.393
4.278ValLeu: 4.278 ± 0.309
1.78ValMet: 1.78 ± 0.216
3.462ValAsn: 3.462 ± 0.373
2.102ValPro: 2.102 ± 0.236
2.003ValGln: 2.003 ± 0.215
3.066ValArg: 3.066 ± 0.24
3.981ValSer: 3.981 ± 0.309
4.278ValThr: 4.278 ± 0.387
6.998ValVal: 6.998 ± 0.444
1.459ValTrp: 1.459 ± 0.201
2.918ValTyr: 2.918 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
0.989TrpAla: 0.989 ± 0.196
0.371TrpCys: 0.371 ± 0.096
1.162TrpAsp: 1.162 ± 0.13
1.731TrpGlu: 1.731 ± 0.205
0.717TrpPhe: 0.717 ± 0.139
1.039TrpGly: 1.039 ± 0.201
0.321TrpHis: 0.321 ± 0.104
1.236TrpIle: 1.236 ± 0.184
1.187TrpLys: 1.187 ± 0.192
1.41TrpLeu: 1.41 ± 0.226
0.42TrpMet: 0.42 ± 0.102
0.866TrpAsn: 0.866 ± 0.168
0.569TrpPro: 0.569 ± 0.114
0.544TrpGln: 0.544 ± 0.121
0.816TrpArg: 0.816 ± 0.144
0.841TrpSer: 0.841 ± 0.153
1.138TrpThr: 1.138 ± 0.152
1.088TrpVal: 1.088 ± 0.135
0.495TrpTrp: 0.495 ± 0.112
0.915TrpTyr: 0.915 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.943TyrAla: 2.943 ± 0.244
0.668TyrCys: 0.668 ± 0.123
3.141TyrAsp: 3.141 ± 0.298
2.003TyrGlu: 2.003 ± 0.258
1.484TyrPhe: 1.484 ± 0.189
3.066TyrGly: 3.066 ± 0.27
0.964TyrHis: 0.964 ± 0.15
1.78TyrIle: 1.78 ± 0.247
2.473TyrLys: 2.473 ± 0.208
3.932TyrLeu: 3.932 ± 0.313
0.94TyrMet: 0.94 ± 0.139
2.324TyrAsn: 2.324 ± 0.239
1.978TyrPro: 1.978 ± 0.221
1.583TyrGln: 1.583 ± 0.198
2.176TyrArg: 2.176 ± 0.246
2.473TyrSer: 2.473 ± 0.27
2.819TyrThr: 2.819 ± 0.251
2.324TyrVal: 2.324 ± 0.212
0.89TyrTrp: 0.89 ± 0.174
2.226TyrTyr: 2.226 ± 0.261
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 206 proteins (40440 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski