Sphingobacterium sp. JB170

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4094 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4K7Y1|A0A1R4K7Y1_9SPHI Putative membrane protein OS=Sphingobacterium sp. JB170 OX=1434842 GN=FM107_10445 PE=3 SV=1
MM1 pKa = 7.67EE2 pKa = 5.91LLKK5 pKa = 10.98AWDD8 pKa = 3.84YY9 pKa = 11.74NILATCGGMALCATCHH25 pKa = 5.12IQVLRR30 pKa = 11.84GAEE33 pKa = 3.98NLNEE37 pKa = 4.56PGDD40 pKa = 4.09MEE42 pKa = 6.85LDD44 pKa = 3.71MLDD47 pKa = 3.81TLPDD51 pKa = 3.28VTDD54 pKa = 3.63QSRR57 pKa = 11.84LACQLRR63 pKa = 11.84LGNEE67 pKa = 3.95NDD69 pKa = 3.42NLSIQIEE76 pKa = 4.46GSLADD81 pKa = 3.78NLSS84 pKa = 3.18

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4K255|A0A1R4K255_9SPHI Endo-1 4-beta-xylanase A OS=Sphingobacterium sp. JB170 OX=1434842 GN=FM107_09620 PE=4 SV=1
MM1 pKa = 7.39SSCLIFPAPSISAYY15 pKa = 8.89RR16 pKa = 11.84DD17 pKa = 2.95KK18 pKa = 11.12YY19 pKa = 10.14GRR21 pKa = 11.84GRR23 pKa = 11.84PGIAQEE29 pKa = 3.82RR30 pKa = 11.84HH31 pKa = 4.31LSKK34 pKa = 10.83RR35 pKa = 11.84ATT37 pKa = 3.4

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4094

0

4094

1274958

37

2131

311.4

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.135 ± 0.039

0.735 ± 0.013

5.578 ± 0.027

6.106 ± 0.037

4.893 ± 0.025

6.875 ± 0.04

1.956 ± 0.019

7.27 ± 0.035

6.465 ± 0.037

9.407 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.017

5.416 ± 0.035

3.668 ± 0.022

3.985 ± 0.022

4.36 ± 0.025

6.571 ± 0.033

5.57 ± 0.031

6.385 ± 0.029

1.195 ± 0.017

4.118 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski