Collinsella sp. CAG:289

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; environmental samples

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7D2U1|R7D2U1_9ACTN Uncharacterized protein OS=Collinsella sp. CAG:289 OX=1262851 GN=BN589_01420 PE=4 SV=1
MM1 pKa = 7.17SQFASVVVSLEE12 pKa = 4.09DD13 pKa = 4.03KK14 pKa = 10.84IEE16 pKa = 4.26NPGDD20 pKa = 3.63TLPVAGHH27 pKa = 6.87IDD29 pKa = 3.3AATYY33 pKa = 10.15KK34 pKa = 10.49AGEE37 pKa = 4.28KK38 pKa = 10.45SFSLDD43 pKa = 3.3DD44 pKa = 4.85GISYY48 pKa = 11.23DD49 pKa = 4.43VMLTNTGDD57 pKa = 3.48GVLATGIVRR66 pKa = 11.84AHH68 pKa = 5.23VTGEE72 pKa = 3.91CDD74 pKa = 2.83RR75 pKa = 11.84CLDD78 pKa = 3.58VAHH81 pKa = 6.67FEE83 pKa = 4.32VAGEE87 pKa = 3.88IEE89 pKa = 4.4EE90 pKa = 4.68YY91 pKa = 11.07YY92 pKa = 10.9LFEE95 pKa = 4.42EE96 pKa = 5.03PEE98 pKa = 4.31DD99 pKa = 4.01PEE101 pKa = 4.54SYY103 pKa = 10.87EE104 pKa = 4.77DD105 pKa = 3.8GFEE108 pKa = 4.19LVSEE112 pKa = 4.76DD113 pKa = 3.46RR114 pKa = 11.84TIDD117 pKa = 3.59LSDD120 pKa = 5.18AIADD124 pKa = 4.54AIVMDD129 pKa = 3.98TPFVVLCRR137 pKa = 11.84PDD139 pKa = 3.59CAGLCPTCGANLNEE153 pKa = 4.64APCACEE159 pKa = 5.68DD160 pKa = 3.88KK161 pKa = 11.33QKK163 pKa = 11.4DD164 pKa = 3.54DD165 pKa = 3.9YY166 pKa = 11.51AASDD170 pKa = 4.08SNPFAALKK178 pKa = 10.13NLKK181 pKa = 9.86FDD183 pKa = 3.83EE184 pKa = 4.51

Molecular weight: 19.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7D8K0|R7D8K0_9ACTN Transcriptional regulator SARP family OS=Collinsella sp. CAG:289 OX=1262851 GN=BN589_01219 PE=4 SV=1
MM1 pKa = 7.65IGHH4 pKa = 6.83HH5 pKa = 6.61CGGFLAVVEE14 pKa = 4.33HH15 pKa = 6.85RR16 pKa = 11.84SIEE19 pKa = 3.72IHH21 pKa = 6.09QRR23 pKa = 11.84DD24 pKa = 3.44ARR26 pKa = 11.84IIKK29 pKa = 10.33LKK31 pKa = 9.67MRR33 pKa = 11.84QHH35 pKa = 6.86RR36 pKa = 11.84DD37 pKa = 3.02LVFRR41 pKa = 11.84PIEE44 pKa = 4.1RR45 pKa = 11.84RR46 pKa = 11.84CHH48 pKa = 6.6DD49 pKa = 3.48IEE51 pKa = 4.82LVGNLLMCKK60 pKa = 9.81RR61 pKa = 11.84RR62 pKa = 11.84QPVLDD67 pKa = 3.57EE68 pKa = 4.29GRR70 pKa = 11.84TYY72 pKa = 9.67TCKK75 pKa = 10.37RR76 pKa = 11.84HH77 pKa = 6.85DD78 pKa = 3.75DD79 pKa = 3.49HH80 pKa = 8.71HH81 pKa = 8.03GDD83 pKa = 4.05GEE85 pKa = 4.41DD86 pKa = 3.67SSKK89 pKa = 9.46HH90 pKa = 4.25TLRR93 pKa = 11.84KK94 pKa = 9.18RR95 pKa = 11.84PMRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84SYY102 pKa = 9.99CRR104 pKa = 11.84RR105 pKa = 11.84HH106 pKa = 4.38IRR108 pKa = 11.84LRR110 pKa = 11.84LVSSKK115 pKa = 10.95AITHH119 pKa = 6.32AAHH122 pKa = 7.12RR123 pKa = 11.84FDD125 pKa = 3.6ARR127 pKa = 11.84GVIAQLLTEE136 pKa = 4.33RR137 pKa = 11.84LHH139 pKa = 8.4VYY141 pKa = 9.01VNCAA145 pKa = 2.91

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1652

0

1652

528494

30

1804

319.9

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.097 ± 0.07

1.7 ± 0.029

6.364 ± 0.054

6.526 ± 0.058

3.634 ± 0.036

7.928 ± 0.057

2.154 ± 0.032

5.563 ± 0.047

4.087 ± 0.056

9.101 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.03

3.149 ± 0.035

4.131 ± 0.046

3.055 ± 0.033

5.88 ± 0.064

6.011 ± 0.054

5.411 ± 0.053

7.796 ± 0.054

0.922 ± 0.019

2.743 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski