Immundisolibacter cernigliae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Immundisolibacterales; Immundisolibacteraceae; Immundisolibacter

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1YSF9|A0A1B1YSF9_9GAMM Uncharacterized protein OS=Immundisolibacter cernigliae OX=1810504 GN=PG2T_05295 PE=4 SV=1
MM1 pKa = 7.41LAATASASLLATDD14 pKa = 4.78AASAGAKK21 pKa = 10.06FSIDD25 pKa = 3.23DD26 pKa = 3.86THH28 pKa = 6.12WVSVGAGLRR37 pKa = 11.84TSFSSVEE44 pKa = 3.98DD45 pKa = 3.59AAPDD49 pKa = 3.79DD50 pKa = 4.07GRR52 pKa = 11.84SNDD55 pKa = 3.46FTVDD59 pKa = 4.2SIRR62 pKa = 11.84LYY64 pKa = 11.4LNAQIHH70 pKa = 5.12QYY72 pKa = 10.57ILLEE76 pKa = 4.42FNTEE80 pKa = 4.06RR81 pKa = 11.84YY82 pKa = 7.8DD83 pKa = 3.87TGDD86 pKa = 3.77DD87 pKa = 3.5DD88 pKa = 6.67DD89 pKa = 5.83IRR91 pKa = 11.84MLDD94 pKa = 4.36AIAKK98 pKa = 7.18FTIAPEE104 pKa = 3.84FNIWAGRR111 pKa = 11.84HH112 pKa = 5.53LPPSDD117 pKa = 3.34RR118 pKa = 11.84ANLDD122 pKa = 2.99GPYY125 pKa = 10.47YY126 pKa = 10.5LNAWTFPIAQAYY138 pKa = 6.76PAIFAGRR145 pKa = 11.84DD146 pKa = 3.08EE147 pKa = 4.54GVSVNGSIDD156 pKa = 3.43EE157 pKa = 4.45GVFGYY162 pKa = 11.27ALGVYY167 pKa = 10.27DD168 pKa = 6.17GMDD171 pKa = 3.05ASYY174 pKa = 11.37SGITDD179 pKa = 4.47LNNPNQDD186 pKa = 3.81DD187 pKa = 4.02NLLFAGRR194 pKa = 11.84LQWALWDD201 pKa = 4.34PEE203 pKa = 4.26PGFYY207 pKa = 7.65TTSSYY212 pKa = 10.87FGEE215 pKa = 4.26KK216 pKa = 10.46NILTFGAAAQYY227 pKa = 10.74QSDD230 pKa = 4.09GTGTMASPGDD240 pKa = 3.66FFGWNVDD247 pKa = 3.24ALLEE251 pKa = 4.24RR252 pKa = 11.84KK253 pKa = 9.89VGDD256 pKa = 3.48GGAVSLEE263 pKa = 3.84GAYY266 pKa = 10.57YY267 pKa = 10.4DD268 pKa = 4.28YY269 pKa = 11.8DD270 pKa = 5.11HH271 pKa = 7.76DD272 pKa = 5.44DD273 pKa = 3.48EE274 pKa = 4.96MPAFGYY280 pKa = 10.02QGNGYY285 pKa = 9.17YY286 pKa = 10.94LLASFLTPQKK296 pKa = 10.65FGIGKK301 pKa = 7.99LQPHH305 pKa = 5.79VRR307 pKa = 11.84YY308 pKa = 9.32QAVDD312 pKa = 3.56DD313 pKa = 5.38DD314 pKa = 4.37NAPDD318 pKa = 3.56HH319 pKa = 7.09DD320 pKa = 4.02RR321 pKa = 11.84WEE323 pKa = 4.51LGLGYY328 pKa = 10.0IIDD331 pKa = 3.95GQKK334 pKa = 10.87AKK336 pKa = 10.97VIATYY341 pKa = 10.35GADD344 pKa = 3.75DD345 pKa = 3.94FDD347 pKa = 5.88GGEE350 pKa = 4.14TTDD353 pKa = 4.64FFILGVQLQII363 pKa = 4.54

Molecular weight: 39.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1YWA4|A0A1B1YWA4_9GAMM EF-P post-translational modification enzyme B OS=Immundisolibacter cernigliae OX=1810504 GN=PG2T_12415 PE=3 SV=1
MM1 pKa = 7.59PRR3 pKa = 11.84TRR5 pKa = 11.84ILTAIVLLGLVLLLLWRR22 pKa = 11.84GTLVWLLGVFAAVALRR38 pKa = 11.84AAWEE42 pKa = 3.99WAGLCSGLSGARR54 pKa = 11.84RR55 pKa = 11.84WLLLATLGASLAGAYY70 pKa = 9.1ALPASWTAVALAAGLAWWALGLRR93 pKa = 11.84LVLCYY98 pKa = 9.6PVLPDD103 pKa = 4.13WIDD106 pKa = 3.43RR107 pKa = 11.84PLAQAAIILLALAPAWLALARR128 pKa = 11.84LADD131 pKa = 3.89GQRR134 pKa = 11.84PLLLLCLALVWAADD148 pKa = 3.3SGAYY152 pKa = 9.95LVGRR156 pKa = 11.84RR157 pKa = 11.84FGRR160 pKa = 11.84RR161 pKa = 11.84KK162 pKa = 9.88LCPQVSPGKK171 pKa = 8.44TVEE174 pKa = 4.08GLLGGLVGAALIGLVAGLALGLDD197 pKa = 4.3GLRR200 pKa = 11.84LAALVGLATACAAVSVVGDD219 pKa = 3.62LAEE222 pKa = 4.35SLFKK226 pKa = 10.75RR227 pKa = 11.84RR228 pKa = 11.84AGVKK232 pKa = 10.0DD233 pKa = 3.7SSHH236 pKa = 6.94LLPGHH241 pKa = 6.33GGVLDD246 pKa = 5.45RR247 pKa = 11.84IDD249 pKa = 4.28ALIAAAPLFALTAPLLVRR267 pKa = 11.84GG268 pKa = 4.23

Molecular weight: 27.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2904

0

2904

932638

41

3242

321.2

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.257 ± 0.074

0.988 ± 0.018

5.754 ± 0.033

5.209 ± 0.039

3.35 ± 0.029

8.823 ± 0.046

2.368 ± 0.026

4.045 ± 0.029

2.315 ± 0.032

11.582 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.022

2.239 ± 0.025

5.583 ± 0.035

3.857 ± 0.028

7.877 ± 0.044

4.451 ± 0.033

4.83 ± 0.039

7.552 ± 0.038

1.427 ± 0.022

2.418 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski