Hathewaya proteolytica DSM 3090

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Hathewaya; Hathewaya proteolytica

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6J4B0|A0A1M6J4B0_9CLOT Ribonuclease Y OS=Hathewaya proteolytica DSM 3090 OX=1121331 GN=rny PE=3 SV=1
MM1 pKa = 7.63IEE3 pKa = 4.31KK4 pKa = 10.04CHH6 pKa = 6.45KK7 pKa = 9.98CHH9 pKa = 6.97SLDD12 pKa = 3.41IVDD15 pKa = 3.55VDD17 pKa = 4.56GVLMCNNCGEE27 pKa = 4.52ILNGDD32 pKa = 4.04CKK34 pKa = 10.97KK35 pKa = 10.57EE36 pKa = 3.69ISIKK40 pKa = 9.76IDD42 pKa = 3.22SVEE45 pKa = 4.12AQNHH49 pKa = 5.65AEE51 pKa = 4.06SSISQDD57 pKa = 2.95DD58 pKa = 3.9DD59 pKa = 4.98LNIEE63 pKa = 4.12ALEE66 pKa = 4.05NLNDD70 pKa = 3.75EE71 pKa = 5.09DD72 pKa = 4.87DD73 pKa = 4.29LSEE76 pKa = 4.23EE77 pKa = 4.55EE78 pKa = 5.01IGDD81 pKa = 3.8FQPFSDD87 pKa = 5.28LSYY90 pKa = 11.34LLDD93 pKa = 4.01SEE95 pKa = 5.52EE96 pKa = 5.06EE97 pKa = 3.97MDD99 pKa = 5.13SDD101 pKa = 5.44QEE103 pKa = 4.34DD104 pKa = 3.66VLNRR108 pKa = 11.84EE109 pKa = 4.41GQWCFIDD116 pKa = 5.73DD117 pKa = 4.1VDD119 pKa = 6.04DD120 pKa = 4.28LAEE123 pKa = 4.44DD124 pKa = 4.99LSYY127 pKa = 11.59EE128 pKa = 4.39DD129 pKa = 4.09YY130 pKa = 11.67NNAYY134 pKa = 8.4EE135 pKa = 4.22EE136 pKa = 4.84KK137 pKa = 10.73YY138 pKa = 10.33PGLLIRR144 pKa = 11.84KK145 pKa = 8.15

Molecular weight: 16.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6MP43|A0A1M6MP43_9CLOT Selenium metabolism protein YedF OS=Hathewaya proteolytica DSM 3090 OX=1121331 GN=SAMN02745248_01090 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.07KK9 pKa = 8.55RR10 pKa = 11.84QRR12 pKa = 11.84QKK14 pKa = 9.41EE15 pKa = 3.76HH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTANGRR28 pKa = 11.84NVLKK32 pKa = 10.56RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.6GRR39 pKa = 11.84KK40 pKa = 8.94KK41 pKa = 9.66LTAA44 pKa = 4.2

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2678

0

2678

832864

39

4443

311.0

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.568 ± 0.045

1.534 ± 0.024

5.571 ± 0.036

7.571 ± 0.057

4.296 ± 0.036

6.308 ± 0.044

1.483 ± 0.02

9.178 ± 0.049

8.888 ± 0.05

8.724 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.06 ± 0.026

6.009 ± 0.04

2.737 ± 0.022

2.662 ± 0.023

3.591 ± 0.029

6.331 ± 0.034

4.882 ± 0.038

6.748 ± 0.037

0.677 ± 0.013

4.18 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski