Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Nitrobacter; Nitrobacter hamburgensis

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1QNN8|Q1QNN8_NITHX Flagellar FlbT OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14) OX=323097 GN=Nham_1335 PE=4 SV=1
MM1 pKa = 7.42GAYY4 pKa = 9.38KK5 pKa = 10.36ISIDD9 pKa = 4.96SITCLSQKK17 pKa = 9.41DD18 pKa = 3.76AKK20 pKa = 10.69GVAEE24 pKa = 4.29VWLLAQSDD32 pKa = 3.86GGPPVRR38 pKa = 11.84YY39 pKa = 8.43PHH41 pKa = 7.12APLSAQNMDD50 pKa = 4.96EE51 pKa = 4.26GDD53 pKa = 3.32TWSIDD58 pKa = 3.08GDD60 pKa = 3.96LVLEE64 pKa = 5.25FDD66 pKa = 4.2GCCNLTIYY74 pKa = 10.26EE75 pKa = 4.04QDD77 pKa = 3.34FNIDD81 pKa = 2.92INATDD86 pKa = 4.09FLGCASFTSGANDD99 pKa = 3.92ANDD102 pKa = 4.18KK103 pKa = 10.62IATNGSDD110 pKa = 4.46SGDD113 pKa = 3.27SDD115 pKa = 3.78YY116 pKa = 11.83SKK118 pKa = 10.95FAISFSWVNN127 pKa = 3.12

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q1QH74|DAPE_NITHX Succinyl-diaminopimelate desuccinylase OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14) OX=323097 GN=dapE PE=3 SV=2
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATTGGRR28 pKa = 11.84KK29 pKa = 8.78ILAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4243

0

4243

1297934

31

2845

305.9

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.179 ± 0.046

0.872 ± 0.012

5.821 ± 0.029

5.261 ± 0.039

3.642 ± 0.025

8.168 ± 0.05

2.116 ± 0.017

5.317 ± 0.024

3.75 ± 0.035

9.598 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.017

2.819 ± 0.03

5.241 ± 0.032

3.172 ± 0.023

7.534 ± 0.047

5.786 ± 0.032

5.49 ± 0.034

7.363 ± 0.031

1.321 ± 0.018

2.211 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski