Salmonella phage SEN1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Eganvirus; Salmonella virus SEN1

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3ULF6|A0A0M3ULF6_9CAUD Putative tail fibers protein OS=Salmonella phage SEN1 OX=1647455 GN=SEN1_19 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.07VRR4 pKa = 11.84SMQGDD9 pKa = 3.84TLDD12 pKa = 4.21VICARR17 pKa = 11.84YY18 pKa = 9.04YY19 pKa = 10.75GRR21 pKa = 11.84TEE23 pKa = 4.12GVVEE27 pKa = 4.42TVLQANPGLSEE38 pKa = 4.6LGVILPHH45 pKa = 6.21GTAIDD50 pKa = 4.16LPDD53 pKa = 3.87VPSSPVTEE61 pKa = 4.95TINLWEE67 pKa = 4.05

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4RCN2|A0A0M4RCN2_9CAUD Lysis protein LysC OS=Salmonella phage SEN1 OX=1647455 GN=SEN1_12 PE=4 SV=1
MM1 pKa = 7.51TNLDD5 pKa = 3.82PRR7 pKa = 11.84QLWRR11 pKa = 11.84RR12 pKa = 11.84TARR15 pKa = 11.84TGKK18 pKa = 10.2SDD20 pKa = 3.01ACKK23 pKa = 10.13TMHH26 pKa = 7.22LLHH29 pKa = 7.04ARR31 pKa = 11.84LKK33 pKa = 10.02NGKK36 pKa = 8.6FAEE39 pKa = 4.31KK40 pKa = 8.53WHH42 pKa = 6.61KK43 pKa = 9.34KK44 pKa = 8.02TGIHH48 pKa = 6.53GAGLNQVYY56 pKa = 10.25

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

9093

39

814

211.5

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.415 ± 0.545

0.979 ± 0.135

6.137 ± 0.303

6.093 ± 0.3

3.09 ± 0.28

6.84 ± 0.372

2.002 ± 0.2

5.51 ± 0.202

5.664 ± 0.315

9.106 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.14

3.915 ± 0.22

4.179 ± 0.223

3.926 ± 0.255

6.346 ± 0.452

6.214 ± 0.231

6.522 ± 0.434

6.412 ± 0.353

1.595 ± 0.15

2.507 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski