Thermobifida phage P318

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0ID44|A0A3T0ID44_9CAUD FAD-dependent thymidylate synthase OS=Thermobifida phage P318 OX=2500138 PE=3 SV=1
MM1 pKa = 7.12STTTTSYY8 pKa = 10.97GHH10 pKa = 5.82WTNIVDD16 pKa = 4.31HH17 pKa = 6.65KK18 pKa = 11.1ASTVGQTVLRR28 pKa = 11.84FLTAADD34 pKa = 4.07PDD36 pKa = 3.74WAEE39 pKa = 3.89EE40 pKa = 4.14LEE42 pKa = 4.43EE43 pKa = 4.58NGVLEE48 pKa = 5.66DD49 pKa = 4.5IIEE52 pKa = 4.32EE53 pKa = 3.96YY54 pKa = 10.93RR55 pKa = 11.84NAINDD60 pKa = 4.03ALPAGIVLAGSEE72 pKa = 4.47FFGPVDD78 pKa = 4.23PDD80 pKa = 3.51PAEE83 pKa = 3.81WDD85 pKa = 2.74GDD87 pKa = 4.23YY88 pKa = 11.08PVTSDD93 pKa = 3.74GRR95 pKa = 11.84LDD97 pKa = 3.13IAAIVAEE104 pKa = 4.17VDD106 pKa = 3.19LGEE109 pKa = 4.33IVAKK113 pKa = 10.29FDD115 pKa = 3.59EE116 pKa = 4.73VDD118 pKa = 3.2PDD120 pKa = 4.08GYY122 pKa = 10.47VAAVATSADD131 pKa = 3.39VVVGNNCVVAVFEE144 pKa = 4.42RR145 pKa = 11.84QVTGYY150 pKa = 10.64RR151 pKa = 11.84SGEE154 pKa = 4.2DD155 pKa = 3.56GEE157 pKa = 4.38EE158 pKa = 4.14TPVYY162 pKa = 10.53SLGDD166 pKa = 3.36IVYY169 pKa = 9.67EE170 pKa = 4.3ADD172 pKa = 2.92TGIPMDD178 pKa = 5.17ADD180 pKa = 3.82DD181 pKa = 4.09ATTRR185 pKa = 11.84ATAAATEE192 pKa = 4.14LLEE195 pKa = 4.16EE196 pKa = 4.74AGWEE200 pKa = 4.3VVSEE204 pKa = 4.04WEE206 pKa = 4.0YY207 pKa = 11.47SDD209 pKa = 3.46NSVYY213 pKa = 11.37AHH215 pKa = 6.57ATRR218 pKa = 11.84HH219 pKa = 5.62NPNQYY224 pKa = 9.05TYY226 pKa = 10.7AIEE229 pKa = 4.02TSVDD233 pKa = 3.41GEE235 pKa = 4.37SWSVEE240 pKa = 4.37VISDD244 pKa = 3.54AQTAEE249 pKa = 4.22TPEE252 pKa = 4.08EE253 pKa = 4.17CASRR257 pKa = 11.84LLVDD261 pKa = 3.47WLGEE265 pKa = 4.13FEE267 pKa = 4.48EE268 pKa = 5.06TPQYY272 pKa = 10.3SRR274 pKa = 11.84CVVWEE279 pKa = 4.08GNIDD283 pKa = 4.11DD284 pKa = 4.27VDD286 pKa = 3.61KK287 pKa = 11.08AAAIAYY293 pKa = 7.92PPDD296 pKa = 4.0SYY298 pKa = 11.33PVTVYY303 pKa = 9.6WSIVRR308 pKa = 11.84GEE310 pKa = 4.25APYY313 pKa = 10.68AAAAEE318 pKa = 4.37PGPLGIPVPDD328 pKa = 3.59AVLEE332 pKa = 4.21LGARR336 pKa = 11.84FGLGQDD342 pKa = 3.13PDD344 pKa = 4.35VYY346 pKa = 11.62VMVAPAEE353 pKa = 4.01LAGPIPGEE361 pKa = 3.51LAYY364 pKa = 10.93YY365 pKa = 10.49RR366 pKa = 11.84GTLPYY371 pKa = 9.66ATALQVWAEE380 pKa = 3.77IQEE383 pKa = 4.22RR384 pKa = 11.84QEE386 pKa = 3.82RR387 pKa = 3.86

Molecular weight: 41.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R573|A0A3Q9R573_9CAUD Minor tail protein OS=Thermobifida phage P318 OX=2500138 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.12HH3 pKa = 4.6SHH5 pKa = 5.02QPIRR9 pKa = 11.84KK10 pKa = 8.24ILRR13 pKa = 11.84NSLPVVEE20 pKa = 5.4KK21 pKa = 10.98VDD23 pKa = 4.43DD24 pKa = 4.17LFFVSALEE32 pKa = 3.91IPRR35 pKa = 11.84NVVDD39 pKa = 4.37RR40 pKa = 11.84LNHH43 pKa = 5.62VSDD46 pKa = 4.52DD47 pKa = 3.84ASSGRR52 pKa = 11.84QIIHH56 pKa = 7.08ANRR59 pKa = 11.84SAPARR64 pKa = 11.84QCIQGRR70 pKa = 11.84QPVGVRR76 pKa = 11.84DD77 pKa = 3.61RR78 pKa = 11.84PGGKK82 pKa = 9.25RR83 pKa = 11.84FNRR86 pKa = 11.84LGRR89 pKa = 11.84QDD91 pKa = 4.23KK92 pKa = 10.86IVDD95 pKa = 3.86FVVNHH100 pKa = 6.14IQPIVFGLLGQGDD113 pKa = 4.86YY114 pKa = 8.91PTEE117 pKa = 4.38IGGEE121 pKa = 4.01INSLLKK127 pKa = 10.7HH128 pKa = 6.13GSCPFQLLTGQISAEE143 pKa = 4.22LKK145 pKa = 10.55PLTPLYY151 pKa = 10.3KK152 pKa = 10.23NPKK155 pKa = 7.87HH156 pKa = 6.34AVGGTPARR164 pKa = 11.84VRR166 pKa = 11.84LGQASLQLCWSDD178 pKa = 3.26VTNDD182 pKa = 2.98SDD184 pKa = 3.8VWGVHH189 pKa = 5.38HH190 pKa = 7.09NRR192 pKa = 11.84LFLSNVRR199 pKa = 11.84KK200 pKa = 10.03ASSFPCFSQVLAQRR214 pKa = 11.84RR215 pKa = 11.84IHH217 pKa = 6.23PVSAKK222 pKa = 8.39YY223 pKa = 9.75RR224 pKa = 11.84RR225 pKa = 11.84HH226 pKa = 6.34FGHH229 pKa = 7.18DD230 pKa = 3.06HH231 pKa = 6.03QSRR234 pKa = 11.84KK235 pKa = 9.74SGKK238 pKa = 9.58KK239 pKa = 9.4PGDD242 pKa = 3.25VFLTVFVFLLHH253 pKa = 6.49HH254 pKa = 6.75PIVLQRR260 pKa = 11.84PTVQISHH267 pKa = 5.54EE268 pKa = 4.06TSPRR272 pKa = 11.84VGGGNEE278 pKa = 3.67VTSPVKK284 pKa = 10.5QVGNRR289 pKa = 11.84LFHH292 pKa = 6.04HH293 pKa = 6.94HH294 pKa = 6.82SGIPCGSTFPANARR308 pKa = 11.84IASQDD313 pKa = 3.32RR314 pKa = 11.84PNGQLLVRR322 pKa = 11.84VTNLDD327 pKa = 2.78GRR329 pKa = 11.84LRR331 pKa = 11.84PNSDD335 pKa = 4.18RR336 pKa = 11.84IHH338 pKa = 5.91GHH340 pKa = 5.56QGHH343 pKa = 6.82EE344 pKa = 4.11DD345 pKa = 3.5ADD347 pKa = 4.13RR348 pKa = 11.84VGLSVRR354 pKa = 11.84VQNVDD359 pKa = 3.28NVSKK363 pKa = 10.72RR364 pKa = 11.84AVEE367 pKa = 3.93NFFARR372 pKa = 11.84DD373 pKa = 3.33GTFNHH378 pKa = 6.92CYY380 pKa = 9.99LRR382 pKa = 11.84SSLFYY387 pKa = 9.26QAKK390 pKa = 7.41WNYY393 pKa = 8.18WINGEE398 pKa = 4.29PPTVCRR404 pKa = 11.84GLSGLCQVTVPDD416 pKa = 4.04VPGFPPTLAAPWRR429 pKa = 11.84TAEE432 pKa = 4.35CPGNKK437 pKa = 9.42LARR440 pKa = 11.84QGSDD444 pKa = 2.71RR445 pKa = 11.84PAPPARR451 pKa = 11.84PP452 pKa = 3.42

Molecular weight: 50.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13907

62

1899

267.4

29.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.405 ± 0.598

0.748 ± 0.126

5.947 ± 0.388

7.313 ± 0.388

3.3 ± 0.124

8.262 ± 0.372

1.826 ± 0.218

5.242 ± 0.238

4.473 ± 0.34

7.644 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.138

3.574 ± 0.164

5.228 ± 0.292

3.056 ± 0.21

6.996 ± 0.286

6.285 ± 0.206

5.544 ± 0.262

8.046 ± 0.289

1.769 ± 0.126

3.243 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski