Tetrasphaera sp. F2B08

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Tetrasphaera; unclassified Tetrasphaera

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I1G518|A0A6I1G518_9MICO Xylulose kinase OS=Tetrasphaera sp. F2B08 OX=2653936 GN=xylB PE=3 SV=1
MM1 pKa = 7.09TRR3 pKa = 11.84STLTRR8 pKa = 11.84STLSVAAIGLLVLTGCSSVQDD29 pKa = 3.72EE30 pKa = 4.99SPAATATTAATSGEE44 pKa = 4.31SGSTAFLEE52 pKa = 4.37QFGLDD57 pKa = 3.51SSDD60 pKa = 3.82DD61 pKa = 3.47PRR63 pKa = 11.84DD64 pKa = 3.14IVTALDD70 pKa = 3.52RR71 pKa = 11.84TNDD74 pKa = 3.63DD75 pKa = 3.23RR76 pKa = 11.84DD77 pKa = 3.83SGVMGSVRR85 pKa = 11.84YY86 pKa = 9.43DD87 pKa = 3.01AVVFTTADD95 pKa = 3.34GEE97 pKa = 4.8TTVPIEE103 pKa = 4.44DD104 pKa = 3.66GFYY107 pKa = 10.74LALAPYY113 pKa = 10.2VDD115 pKa = 3.93QTHH118 pKa = 6.95DD119 pKa = 3.56CYY121 pKa = 11.57YY122 pKa = 10.93HH123 pKa = 7.07NLATCQGEE131 pKa = 4.5LVGEE135 pKa = 4.42DD136 pKa = 3.71LDD138 pKa = 4.29VTITTDD144 pKa = 3.4DD145 pKa = 4.13GEE147 pKa = 4.61VLVDD151 pKa = 3.77EE152 pKa = 4.97TVTTYY157 pKa = 11.67DD158 pKa = 3.12NGFVGFWLPRR168 pKa = 11.84DD169 pKa = 3.28ITGTIEE175 pKa = 3.84VTHH178 pKa = 7.25DD179 pKa = 3.52GRR181 pKa = 11.84SVTAPIATGPDD192 pKa = 3.51DD193 pKa = 4.02PTCVTTLQLTT203 pKa = 3.87

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I1GDH8|A0A6I1GDH8_9MICO L-2-hydroxyglutarate oxidase OS=Tetrasphaera sp. F2B08 OX=2653936 GN=lhgO PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3963

0

3963

1326481

26

2400

334.7

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.929 ± 0.054

0.702 ± 0.011

6.395 ± 0.034

5.954 ± 0.036

2.727 ± 0.022

9.141 ± 0.03

2.258 ± 0.02

3.851 ± 0.027

1.575 ± 0.024

10.103 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.015

1.667 ± 0.022

5.757 ± 0.031

2.628 ± 0.02

7.984 ± 0.051

5.471 ± 0.027

6.225 ± 0.029

9.288 ± 0.04

1.539 ± 0.016

1.903 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski