Amino acid dipepetide frequency for Azoarcus sp. CC-YHH848

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.516AlaAla: 20.516 ± 0.218
1.387AlaCys: 1.387 ± 0.033
7.525AlaAsp: 7.525 ± 0.086
8.405AlaGlu: 8.405 ± 0.105
4.14AlaPhe: 4.14 ± 0.057
12.726AlaGly: 12.726 ± 0.142
2.808AlaHis: 2.808 ± 0.05
5.059AlaIle: 5.059 ± 0.072
2.882AlaLys: 2.882 ± 0.056
15.715AlaLeu: 15.715 ± 0.147
2.845AlaMet: 2.845 ± 0.052
2.722AlaAsn: 2.722 ± 0.048
6.207AlaPro: 6.207 ± 0.085
4.545AlaGln: 4.545 ± 0.064
10.768AlaArg: 10.768 ± 0.125
5.598AlaSer: 5.598 ± 0.075
5.757AlaThr: 5.757 ± 0.092
9.501AlaVal: 9.501 ± 0.088
1.998AlaTrp: 1.998 ± 0.047
2.446AlaTyr: 2.446 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.219CysAla: 1.219 ± 0.034
0.127CysCys: 0.127 ± 0.009
0.496CysAsp: 0.496 ± 0.019
0.491CysGlu: 0.491 ± 0.02
0.295CysPhe: 0.295 ± 0.015
1.03CysGly: 1.03 ± 0.032
0.333CysHis: 0.333 ± 0.019
0.376CysIle: 0.376 ± 0.016
0.177CysLys: 0.177 ± 0.011
0.913CysLeu: 0.913 ± 0.028
0.176CysMet: 0.176 ± 0.014
0.217CysAsn: 0.217 ± 0.011
0.531CysPro: 0.531 ± 0.023
0.217CysGln: 0.217 ± 0.012
0.752CysArg: 0.752 ± 0.023
0.438CysSer: 0.438 ± 0.02
0.477CysThr: 0.477 ± 0.02
0.651CysVal: 0.651 ± 0.024
0.123CysTrp: 0.123 ± 0.011
0.23CysTyr: 0.23 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.176AspAla: 7.176 ± 0.089
0.501AspCys: 0.501 ± 0.023
2.964AspAsp: 2.964 ± 0.062
3.723AspGlu: 3.723 ± 0.062
2.164AspPhe: 2.164 ± 0.041
5.363AspGly: 5.363 ± 0.08
1.187AspHis: 1.187 ± 0.03
2.55AspIle: 2.55 ± 0.048
1.508AspLys: 1.508 ± 0.043
5.71AspLeu: 5.71 ± 0.071
1.07AspMet: 1.07 ± 0.027
1.281AspAsn: 1.281 ± 0.035
3.18AspPro: 3.18 ± 0.051
1.385AspGln: 1.385 ± 0.031
3.679AspArg: 3.679 ± 0.057
2.118AspSer: 2.118 ± 0.041
2.768AspThr: 2.768 ± 0.066
3.961AspVal: 3.961 ± 0.065
1.013AspTrp: 1.013 ± 0.028
1.533AspTyr: 1.533 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
8.077GluAla: 8.077 ± 0.085
0.445GluCys: 0.445 ± 0.018
2.732GluAsp: 2.732 ± 0.053
3.184GluGlu: 3.184 ± 0.06
1.836GluPhe: 1.836 ± 0.039
4.282GluGly: 4.282 ± 0.049
1.53GluHis: 1.53 ± 0.028
3.008GluIle: 3.008 ± 0.053
1.781GluLys: 1.781 ± 0.042
6.61GluLeu: 6.61 ± 0.076
1.27GluMet: 1.27 ± 0.033
1.426GluAsn: 1.426 ± 0.035
2.619GluPro: 2.619 ± 0.047
2.408GluGln: 2.408 ± 0.044
5.825GluArg: 5.825 ± 0.074
2.53GluSer: 2.53 ± 0.047
2.886GluThr: 2.886 ± 0.053
4.417GluVal: 4.417 ± 0.064
0.827GluTrp: 0.827 ± 0.023
1.247GluTyr: 1.247 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.495PheAla: 4.495 ± 0.06
0.358PheCys: 0.358 ± 0.016
2.529PheAsp: 2.529 ± 0.041
2.126PheGlu: 2.126 ± 0.045
1.39PhePhe: 1.39 ± 0.037
3.429PheGly: 3.429 ± 0.045
0.836PheHis: 0.836 ± 0.026
1.457PheIle: 1.457 ± 0.036
0.843PheLys: 0.843 ± 0.025
3.095PheLeu: 3.095 ± 0.048
0.693PheMet: 0.693 ± 0.024
0.975PheAsn: 0.975 ± 0.031
1.521PhePro: 1.521 ± 0.034
0.923PheGln: 0.923 ± 0.027
2.293PheArg: 2.293 ± 0.046
1.996PheSer: 1.996 ± 0.042
1.715PheThr: 1.715 ± 0.035
2.738PheVal: 2.738 ± 0.046
0.457PheTrp: 0.457 ± 0.021
0.831PheTyr: 0.831 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
9.819GlyAla: 9.819 ± 0.114
1.006GlyCys: 1.006 ± 0.031
4.414GlyAsp: 4.414 ± 0.08
5.792GlyGlu: 5.792 ± 0.072
3.283GlyPhe: 3.283 ± 0.054
8.029GlyGly: 8.029 ± 0.164
2.032GlyHis: 2.032 ± 0.043
4.111GlyIle: 4.111 ± 0.058
2.962GlyLys: 2.962 ± 0.048
9.546GlyLeu: 9.546 ± 0.097
2.225GlyMet: 2.225 ± 0.046
2.287GlyAsn: 2.287 ± 0.054
2.995GlyPro: 2.995 ± 0.061
2.956GlyGln: 2.956 ± 0.05
7.179GlyArg: 7.179 ± 0.086
4.477GlySer: 4.477 ± 0.087
4.615GlyThr: 4.615 ± 0.104
6.518GlyVal: 6.518 ± 0.077
1.425GlyTrp: 1.425 ± 0.039
2.365GlyTyr: 2.365 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.931HisAla: 2.931 ± 0.049
0.277HisCys: 0.277 ± 0.014
1.28HisAsp: 1.28 ± 0.031
1.219HisGlu: 1.219 ± 0.029
0.917HisPhe: 0.917 ± 0.025
2.428HisGly: 2.428 ± 0.045
0.666HisHis: 0.666 ± 0.023
0.884HisIle: 0.884 ± 0.026
0.505HisLys: 0.505 ± 0.022
2.518HisLeu: 2.518 ± 0.046
0.424HisMet: 0.424 ± 0.02
0.501HisAsn: 0.501 ± 0.017
1.623HisPro: 1.623 ± 0.037
0.623HisGln: 0.623 ± 0.022
1.728HisArg: 1.728 ± 0.035
0.925HisSer: 0.925 ± 0.03
1.04HisThr: 1.04 ± 0.027
1.567HisVal: 1.567 ± 0.036
0.392HisTrp: 0.392 ± 0.015
0.669HisTyr: 0.669 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.132IleAla: 6.132 ± 0.073
0.393IleCys: 0.393 ± 0.018
3.015IleAsp: 3.015 ± 0.045
3.102IleGlu: 3.102 ± 0.053
1.256IlePhe: 1.256 ± 0.037
4.216IleGly: 4.216 ± 0.063
1.003IleHis: 1.003 ± 0.028
1.44IleIle: 1.44 ± 0.037
1.157IleLys: 1.157 ± 0.03
3.716IleLeu: 3.716 ± 0.056
0.694IleMet: 0.694 ± 0.025
1.354IleAsn: 1.354 ± 0.032
2.108IlePro: 2.108 ± 0.044
1.149IleGln: 1.149 ± 0.026
3.097IleArg: 3.097 ± 0.045
2.087IleSer: 2.087 ± 0.037
2.252IleThr: 2.252 ± 0.069
3.55IleVal: 3.55 ± 0.059
0.405IleTrp: 0.405 ± 0.019
0.948IleTyr: 0.948 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
3.271LysAla: 3.271 ± 0.056
0.152LysCys: 0.152 ± 0.013
1.342LysAsp: 1.342 ± 0.035
1.38LysGlu: 1.38 ± 0.04
0.722LysPhe: 0.722 ± 0.026
2.068LysGly: 2.068 ± 0.049
0.574LysHis: 0.574 ± 0.021
1.265LysIle: 1.265 ± 0.031
1.051LysLys: 1.051 ± 0.031
2.932LysLeu: 2.932 ± 0.053
0.585LysMet: 0.585 ± 0.026
0.766LysAsn: 0.766 ± 0.026
1.594LysPro: 1.594 ± 0.044
0.91LysGln: 0.91 ± 0.026
1.946LysArg: 1.946 ± 0.042
1.376LysSer: 1.376 ± 0.035
1.446LysThr: 1.446 ± 0.036
2.208LysVal: 2.208 ± 0.045
0.321LysTrp: 0.321 ± 0.015
0.649LysTyr: 0.649 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
17.086LeuAla: 17.086 ± 0.184
0.992LeuCys: 0.992 ± 0.029
6.559LeuAsp: 6.559 ± 0.067
5.879LeuGlu: 5.879 ± 0.074
3.704LeuPhe: 3.704 ± 0.064
9.051LeuGly: 9.051 ± 0.096
2.389LeuHis: 2.389 ± 0.049
4.394LeuIle: 4.394 ± 0.064
3.042LeuLys: 3.042 ± 0.063
11.846LeuLeu: 11.846 ± 0.137
2.14LeuMet: 2.14 ± 0.043
2.549LeuAsn: 2.549 ± 0.046
6.588LeuPro: 6.588 ± 0.089
3.578LeuGln: 3.578 ± 0.044
8.529LeuArg: 8.529 ± 0.103
5.531LeuSer: 5.531 ± 0.072
5.51LeuThr: 5.51 ± 0.071
7.732LeuVal: 7.732 ± 0.079
1.292LeuTrp: 1.292 ± 0.033
2.229LeuTyr: 2.229 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.519MetAla: 2.519 ± 0.043
0.143MetCys: 0.143 ± 0.01
1.058MetAsp: 1.058 ± 0.031
1.074MetGlu: 1.074 ± 0.03
0.589MetPhe: 0.589 ± 0.024
1.573MetGly: 1.573 ± 0.04
0.453MetHis: 0.453 ± 0.021
0.892MetIle: 0.892 ± 0.03
0.863MetLys: 0.863 ± 0.024
2.389MetLeu: 2.389 ± 0.045
0.483MetMet: 0.483 ± 0.021
0.793MetAsn: 0.793 ± 0.025
1.295MetPro: 1.295 ± 0.033
0.821MetGln: 0.821 ± 0.028
1.51MetArg: 1.51 ± 0.037
1.38MetSer: 1.38 ± 0.034
1.206MetThr: 1.206 ± 0.033
1.483MetVal: 1.483 ± 0.033
0.197MetTrp: 0.197 ± 0.012
0.331MetTyr: 0.331 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.029AsnAla: 3.029 ± 0.058
0.246AsnCys: 0.246 ± 0.015
1.261AsnAsp: 1.261 ± 0.043
1.166AsnGlu: 1.166 ± 0.028
0.866AsnPhe: 0.866 ± 0.026
2.256AsnGly: 2.256 ± 0.049
0.503AsnHis: 0.503 ± 0.016
1.133AsnIle: 1.133 ± 0.031
0.624AsnLys: 0.624 ± 0.022
2.692AsnLeu: 2.692 ± 0.047
0.516AsnMet: 0.516 ± 0.019
0.658AsnAsn: 0.658 ± 0.023
1.767AsnPro: 1.767 ± 0.039
0.762AsnGln: 0.762 ± 0.026
1.825AsnArg: 1.825 ± 0.029
0.99AsnSer: 0.99 ± 0.029
1.298AsnThr: 1.298 ± 0.035
1.842AsnVal: 1.842 ± 0.041
0.415AsnTrp: 0.415 ± 0.018
0.659AsnTyr: 0.659 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
8.047ProAla: 8.047 ± 0.111
0.425ProCys: 0.425 ± 0.017
3.3ProAsp: 3.3 ± 0.052
3.581ProGlu: 3.581 ± 0.058
1.81ProPhe: 1.81 ± 0.039
4.676ProGly: 4.676 ± 0.073
1.164ProHis: 1.164 ± 0.031
1.784ProIle: 1.784 ± 0.04
1.165ProLys: 1.165 ± 0.035
5.435ProLeu: 5.435 ± 0.07
1.01ProMet: 1.01 ± 0.028
1.117ProAsn: 1.117 ± 0.034
3.219ProPro: 3.219 ± 0.077
1.934ProGln: 1.934 ± 0.038
3.327ProArg: 3.327 ± 0.059
2.266ProSer: 2.266 ± 0.039
2.171ProThr: 2.171 ± 0.039
4.329ProVal: 4.329 ± 0.059
0.767ProTrp: 0.767 ± 0.027
1.136ProTyr: 1.136 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.748GlnAla: 4.748 ± 0.068
0.232GlnCys: 0.232 ± 0.013
1.437GlnAsp: 1.437 ± 0.031
1.627GlnGlu: 1.627 ± 0.037
1.064GlnPhe: 1.064 ± 0.032
2.7GlnGly: 2.7 ± 0.045
0.779GlnHis: 0.779 ± 0.023
1.559GlnIle: 1.559 ± 0.036
0.895GlnLys: 0.895 ± 0.026
3.296GlnLeu: 3.296 ± 0.046
0.771GlnMet: 0.771 ± 0.024
0.748GlnAsn: 0.748 ± 0.027
1.973GlnPro: 1.973 ± 0.038
1.44GlnGln: 1.44 ± 0.037
2.855GlnArg: 2.855 ± 0.049
1.536GlnSer: 1.536 ± 0.036
1.602GlnThr: 1.602 ± 0.035
2.596GlnVal: 2.596 ± 0.053
0.513GlnTrp: 0.513 ± 0.019
0.727GlnTyr: 0.727 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.97ArgAla: 8.97 ± 0.108
0.649ArgCys: 0.649 ± 0.022
4.07ArgAsp: 4.07 ± 0.059
4.949ArgGlu: 4.949 ± 0.07
3.142ArgPhe: 3.142 ± 0.053
5.429ArgGly: 5.429 ± 0.064
2.216ArgHis: 2.216 ± 0.042
4.22ArgIle: 4.22 ± 0.052
1.998ArgLys: 1.998 ± 0.047
9.546ArgLeu: 9.546 ± 0.121
1.885ArgMet: 1.885 ± 0.037
2.017ArgAsn: 2.017 ± 0.041
3.994ArgPro: 3.994 ± 0.067
2.868ArgGln: 2.868 ± 0.05
6.694ArgArg: 6.694 ± 0.095
3.491ArgSer: 3.491 ± 0.05
3.42ArgThr: 3.42 ± 0.056
5.363ArgVal: 5.363 ± 0.074
1.171ArgTrp: 1.171 ± 0.031
2.095ArgTyr: 2.095 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.9SerAla: 5.9 ± 0.08
0.423SerCys: 0.423 ± 0.017
2.215SerAsp: 2.215 ± 0.048
2.264SerGlu: 2.264 ± 0.046
1.897SerPhe: 1.897 ± 0.039
5.222SerGly: 5.222 ± 0.097
1.043SerHis: 1.043 ± 0.027
2.158SerIle: 2.158 ± 0.046
1.12SerLys: 1.12 ± 0.029
5.119SerLeu: 5.119 ± 0.061
0.979SerMet: 0.979 ± 0.028
1.181SerAsn: 1.181 ± 0.035
2.572SerPro: 2.572 ± 0.039
1.338SerGln: 1.338 ± 0.032
3.504SerArg: 3.504 ± 0.053
2.449SerSer: 2.449 ± 0.05
2.466SerThr: 2.466 ± 0.05
3.473SerVal: 3.473 ± 0.051
0.676SerTrp: 0.676 ± 0.022
1.137SerTyr: 1.137 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
5.991ThrAla: 5.991 ± 0.082
0.415ThrCys: 0.415 ± 0.018
2.48ThrAsp: 2.48 ± 0.05
2.524ThrGlu: 2.524 ± 0.05
1.48ThrPhe: 1.48 ± 0.033
4.726ThrGly: 4.726 ± 0.091
1.086ThrHis: 1.086 ± 0.032
2.045ThrIle: 2.045 ± 0.055
0.946ThrLys: 0.946 ± 0.03
6.252ThrLeu: 6.252 ± 0.093
0.86ThrMet: 0.86 ± 0.026
1.103ThrAsn: 1.103 ± 0.034
3.137ThrPro: 3.137 ± 0.052
1.435ThrGln: 1.435 ± 0.033
3.551ThrArg: 3.551 ± 0.057
2.123ThrSer: 2.123 ± 0.05
2.467ThrThr: 2.467 ± 0.066
4.298ThrVal: 4.298 ± 0.095
0.674ThrTrp: 0.674 ± 0.021
1.046ThrTyr: 1.046 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
9.669ValAla: 9.669 ± 0.093
0.722ValCys: 0.722 ± 0.024
4.262ValAsp: 4.262 ± 0.063
4.673ValGlu: 4.673 ± 0.059
2.799ValPhe: 2.799 ± 0.049
5.757ValGly: 5.757 ± 0.068
1.609ValHis: 1.609 ± 0.037
3.346ValIle: 3.346 ± 0.052
2.047ValLys: 2.047 ± 0.051
8.584ValLeu: 8.584 ± 0.097
1.663ValMet: 1.663 ± 0.042
1.797ValAsn: 1.797 ± 0.048
3.914ValPro: 3.914 ± 0.06
2.325ValGln: 2.325 ± 0.037
5.672ValArg: 5.672 ± 0.075
4.015ValSer: 4.015 ± 0.062
3.708ValThr: 3.708 ± 0.073
6.192ValVal: 6.192 ± 0.088
0.937ValTrp: 0.937 ± 0.027
1.446ValTyr: 1.446 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.337TrpAla: 1.337 ± 0.034
0.135TrpCys: 0.135 ± 0.011
0.583TrpAsp: 0.583 ± 0.023
0.641TrpGlu: 0.641 ± 0.022
0.508TrpPhe: 0.508 ± 0.018
0.891TrpGly: 0.891 ± 0.031
0.388TrpHis: 0.388 ± 0.016
0.628TrpIle: 0.628 ± 0.025
0.398TrpLys: 0.398 ± 0.018
2.168TrpLeu: 2.168 ± 0.047
0.376TrpMet: 0.376 ± 0.016
0.425TrpAsn: 0.425 ± 0.019
0.684TrpPro: 0.684 ± 0.025
0.765TrpGln: 0.765 ± 0.026
1.353TrpArg: 1.353 ± 0.036
0.709TrpSer: 0.709 ± 0.023
0.651TrpThr: 0.651 ± 0.024
1.022TrpVal: 1.022 ± 0.027
0.237TrpTrp: 0.237 ± 0.014
0.334TrpTyr: 0.334 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.614TyrAla: 2.614 ± 0.041
0.242TyrCys: 0.242 ± 0.014
1.309TyrAsp: 1.309 ± 0.033
1.14TyrGlu: 1.14 ± 0.029
0.839TyrPhe: 0.839 ± 0.026
2.184TyrGly: 2.184 ± 0.05
0.516TyrHis: 0.516 ± 0.021
0.767TyrIle: 0.767 ± 0.024
0.594TyrLys: 0.594 ± 0.021
2.535TyrLeu: 2.535 ± 0.041
0.388TyrMet: 0.388 ± 0.015
0.593TyrAsn: 0.593 ± 0.022
1.143TyrPro: 1.143 ± 0.032
0.717TyrGln: 0.717 ± 0.025
2.103TyrArg: 2.103 ± 0.042
1.099TyrSer: 1.099 ± 0.03
1.183TyrThr: 1.183 ± 0.036
1.696TyrVal: 1.696 ± 0.034
0.394TyrTrp: 0.394 ± 0.017
0.596TyrTyr: 0.596 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4218 proteins (1398363 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski