Amino acid dipepetide frequency for Microbacterium phage Margaery

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.775AlaAla: 17.775 ± 1.63
1.114AlaCys: 1.114 ± 0.314
8.525AlaAsp: 8.525 ± 0.865
11.032AlaGlu: 11.032 ± 1.101
2.897AlaPhe: 2.897 ± 0.366
9.751AlaGly: 9.751 ± 1.05
2.396AlaHis: 2.396 ± 0.39
5.349AlaIle: 5.349 ± 0.438
3.065AlaLys: 3.065 ± 0.462
12.537AlaLeu: 12.537 ± 1.08
3.232AlaMet: 3.232 ± 0.396
3.176AlaAsn: 3.176 ± 0.393
7.355AlaPro: 7.355 ± 0.841
4.012AlaGln: 4.012 ± 0.401
9.862AlaArg: 9.862 ± 0.857
6.575AlaSer: 6.575 ± 0.663
7.522AlaThr: 7.522 ± 0.629
7.021AlaVal: 7.021 ± 0.669
2.675AlaTrp: 2.675 ± 0.398
2.619AlaTyr: 2.619 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
0.78CysAla: 0.78 ± 0.222
0.111CysCys: 0.111 ± 0.128
0.501CysAsp: 0.501 ± 0.159
0.613CysGlu: 0.613 ± 0.188
0.111CysPhe: 0.111 ± 0.086
1.727CysGly: 1.727 ± 0.441
0.39CysHis: 0.39 ± 0.139
0.446CysIle: 0.446 ± 0.167
0.111CysLys: 0.111 ± 0.075
0.111CysLeu: 0.111 ± 0.074
0.056CysMet: 0.056 ± 0.058
0.167CysAsn: 0.167 ± 0.089
1.17CysPro: 1.17 ± 0.256
0.223CysGln: 0.223 ± 0.117
0.836CysArg: 0.836 ± 0.222
0.501CysSer: 0.501 ± 0.171
0.279CysThr: 0.279 ± 0.136
0.501CysVal: 0.501 ± 0.171
0.167CysTrp: 0.167 ± 0.099
0.223CysTyr: 0.223 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
9.138AspAla: 9.138 ± 0.891
0.892AspCys: 0.892 ± 0.245
5.405AspAsp: 5.405 ± 0.92
5.126AspGlu: 5.126 ± 0.605
1.56AspPhe: 1.56 ± 0.297
7.912AspGly: 7.912 ± 0.785
1.059AspHis: 1.059 ± 0.215
2.452AspIle: 2.452 ± 0.451
0.613AspLys: 0.613 ± 0.188
4.346AspLeu: 4.346 ± 0.586
1.282AspMet: 1.282 ± 0.268
1.393AspAsn: 1.393 ± 0.246
4.179AspPro: 4.179 ± 0.45
1.226AspGln: 1.226 ± 0.251
4.012AspArg: 4.012 ± 0.546
2.73AspSer: 2.73 ± 0.416
3.232AspThr: 3.232 ± 0.389
4.848AspVal: 4.848 ± 0.629
1.17AspTrp: 1.17 ± 0.232
1.337AspTyr: 1.337 ± 0.29
0.0AspXaa: 0.0 ± 0.0
Glu
8.358GluAla: 8.358 ± 0.643
0.557GluCys: 0.557 ± 0.175
4.346GluAsp: 4.346 ± 0.782
6.185GluGlu: 6.185 ± 0.682
1.504GluPhe: 1.504 ± 0.287
4.29GluGly: 4.29 ± 0.433
2.953GluHis: 2.953 ± 0.443
3.956GluIle: 3.956 ± 0.541
1.894GluLys: 1.894 ± 0.325
3.232GluLeu: 3.232 ± 0.444
2.117GluMet: 2.117 ± 0.283
1.894GluAsn: 1.894 ± 0.357
5.015GluPro: 5.015 ± 0.762
3.12GluGln: 3.12 ± 0.454
6.909GluArg: 6.909 ± 0.703
2.34GluSer: 2.34 ± 0.324
4.068GluThr: 4.068 ± 0.464
4.903GluVal: 4.903 ± 0.55
1.95GluTrp: 1.95 ± 0.322
1.783GluTyr: 1.783 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.507PheAla: 2.507 ± 0.42
0.334PheCys: 0.334 ± 0.148
1.616PheAsp: 1.616 ± 0.329
1.783PheGlu: 1.783 ± 0.287
0.39PhePhe: 0.39 ± 0.176
2.396PheGly: 2.396 ± 0.334
0.613PheHis: 0.613 ± 0.17
0.78PheIle: 0.78 ± 0.185
0.39PheLys: 0.39 ± 0.144
1.504PheLeu: 1.504 ± 0.264
0.724PheMet: 0.724 ± 0.195
0.334PheAsn: 0.334 ± 0.124
1.003PhePro: 1.003 ± 0.218
0.669PheGln: 0.669 ± 0.258
1.56PheArg: 1.56 ± 0.308
0.669PheSer: 0.669 ± 0.238
2.507PheThr: 2.507 ± 0.37
1.114PheVal: 1.114 ± 0.263
0.39PheTrp: 0.39 ± 0.178
0.334PheTyr: 0.334 ± 0.138
0.0PheXaa: 0.0 ± 0.0
Gly
9.472GlyAla: 9.472 ± 0.968
0.836GlyCys: 0.836 ± 0.262
5.683GlyAsp: 5.683 ± 0.628
6.296GlyGlu: 6.296 ± 0.554
2.229GlyPhe: 2.229 ± 0.366
8.469GlyGly: 8.469 ± 1.004
1.894GlyHis: 1.894 ± 0.367
4.458GlyIle: 4.458 ± 0.554
2.842GlyLys: 2.842 ± 0.359
6.241GlyLeu: 6.241 ± 0.822
2.73GlyMet: 2.73 ± 0.386
2.786GlyAsn: 2.786 ± 0.457
3.845GlyPro: 3.845 ± 0.502
2.34GlyGln: 2.34 ± 0.385
5.795GlyArg: 5.795 ± 0.62
4.402GlySer: 4.402 ± 0.595
6.965GlyThr: 6.965 ± 0.665
6.018GlyVal: 6.018 ± 0.469
2.507GlyTrp: 2.507 ± 0.397
2.786GlyTyr: 2.786 ± 0.458
0.0GlyXaa: 0.0 ± 0.0
His
2.34HisAla: 2.34 ± 0.398
0.167HisCys: 0.167 ± 0.097
2.062HisAsp: 2.062 ± 0.388
2.062HisGlu: 2.062 ± 0.355
0.223HisPhe: 0.223 ± 0.108
2.34HisGly: 2.34 ± 0.392
0.446HisHis: 0.446 ± 0.18
0.947HisIle: 0.947 ± 0.2
0.334HisLys: 0.334 ± 0.135
1.839HisLeu: 1.839 ± 0.337
0.39HisMet: 0.39 ± 0.164
0.501HisAsn: 0.501 ± 0.154
0.836HisPro: 0.836 ± 0.23
0.167HisGln: 0.167 ± 0.092
1.672HisArg: 1.672 ± 0.341
0.836HisSer: 0.836 ± 0.181
1.282HisThr: 1.282 ± 0.342
1.449HisVal: 1.449 ± 0.288
0.167HisTrp: 0.167 ± 0.103
0.501HisTyr: 0.501 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
6.854IleAla: 6.854 ± 0.693
0.111IleCys: 0.111 ± 0.077
3.343IleAsp: 3.343 ± 0.45
3.845IleGlu: 3.845 ± 0.427
0.836IlePhe: 0.836 ± 0.229
4.123IleGly: 4.123 ± 0.543
1.114IleHis: 1.114 ± 0.295
2.452IleIle: 2.452 ± 0.541
0.947IleLys: 0.947 ± 0.231
2.619IleLeu: 2.619 ± 0.384
0.78IleMet: 0.78 ± 0.205
1.114IleAsn: 1.114 ± 0.228
3.455IlePro: 3.455 ± 0.599
1.672IleGln: 1.672 ± 0.323
3.065IleArg: 3.065 ± 0.417
2.396IleSer: 2.396 ± 0.333
5.293IleThr: 5.293 ± 0.611
3.622IleVal: 3.622 ± 0.414
0.501IleTrp: 0.501 ± 0.154
0.78IleTyr: 0.78 ± 0.195
0.0IleXaa: 0.0 ± 0.0
Lys
4.458LysAla: 4.458 ± 0.506
0.111LysCys: 0.111 ± 0.077
1.672LysAsp: 1.672 ± 0.285
0.557LysGlu: 0.557 ± 0.153
0.334LysPhe: 0.334 ± 0.167
2.675LysGly: 2.675 ± 0.395
0.724LysHis: 0.724 ± 0.186
1.003LysIle: 1.003 ± 0.212
0.111LysLys: 0.111 ± 0.083
0.613LysLeu: 0.613 ± 0.308
0.78LysMet: 0.78 ± 0.188
0.78LysAsn: 0.78 ± 0.19
1.672LysPro: 1.672 ± 0.346
0.446LysGln: 0.446 ± 0.149
2.73LysArg: 2.73 ± 0.496
1.393LysSer: 1.393 ± 0.332
0.892LysThr: 0.892 ± 0.288
2.34LysVal: 2.34 ± 0.426
0.557LysTrp: 0.557 ± 0.176
0.613LysTyr: 0.613 ± 0.167
0.0LysXaa: 0.0 ± 0.0
Leu
9.361LeuAla: 9.361 ± 0.736
0.557LeuCys: 0.557 ± 0.222
4.402LeuAsp: 4.402 ± 0.486
3.51LeuGlu: 3.51 ± 0.43
1.839LeuPhe: 1.839 ± 0.564
6.352LeuGly: 6.352 ± 0.974
0.892LeuHis: 0.892 ± 0.263
3.51LeuIle: 3.51 ± 0.695
0.78LeuLys: 0.78 ± 0.233
5.795LeuLeu: 5.795 ± 0.758
1.114LeuMet: 1.114 ± 0.216
1.56LeuAsn: 1.56 ± 0.313
4.012LeuPro: 4.012 ± 0.411
2.117LeuGln: 2.117 ± 0.349
5.126LeuArg: 5.126 ± 0.543
4.346LeuSer: 4.346 ± 0.725
6.575LeuThr: 6.575 ± 0.815
5.015LeuVal: 5.015 ± 0.624
1.114LeuTrp: 1.114 ± 0.219
1.449LeuTyr: 1.449 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
2.786MetAla: 2.786 ± 0.335
0.167MetCys: 0.167 ± 0.093
1.393MetAsp: 1.393 ± 0.268
1.003MetGlu: 1.003 ± 0.255
0.167MetPhe: 0.167 ± 0.111
1.56MetGly: 1.56 ± 0.307
0.446MetHis: 0.446 ± 0.14
1.616MetIle: 1.616 ± 0.271
0.724MetLys: 0.724 ± 0.217
1.393MetLeu: 1.393 ± 0.359
0.279MetMet: 0.279 ± 0.125
0.892MetAsn: 0.892 ± 0.17
2.006MetPro: 2.006 ± 0.307
0.39MetGln: 0.39 ± 0.166
1.449MetArg: 1.449 ± 0.308
2.229MetSer: 2.229 ± 0.319
2.842MetThr: 2.842 ± 0.36
1.114MetVal: 1.114 ± 0.238
0.446MetTrp: 0.446 ± 0.173
0.501MetTyr: 0.501 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
3.176AsnAla: 3.176 ± 0.557
0.279AsnCys: 0.279 ± 0.126
0.836AsnAsp: 0.836 ± 0.199
1.56AsnGlu: 1.56 ± 0.364
0.557AsnPhe: 0.557 ± 0.186
2.73AsnGly: 2.73 ± 0.364
0.724AsnHis: 0.724 ± 0.22
1.226AsnIle: 1.226 ± 0.278
0.557AsnLys: 0.557 ± 0.23
1.56AsnLeu: 1.56 ± 0.237
0.446AsnMet: 0.446 ± 0.132
0.557AsnAsn: 0.557 ± 0.194
2.619AsnPro: 2.619 ± 0.471
0.334AsnGln: 0.334 ± 0.128
1.894AsnArg: 1.894 ± 0.292
1.337AsnSer: 1.337 ± 0.278
1.56AsnThr: 1.56 ± 0.306
2.34AsnVal: 2.34 ± 0.296
0.557AsnTrp: 0.557 ± 0.17
0.334AsnTyr: 0.334 ± 0.127
0.0AsnXaa: 0.0 ± 0.0
Pro
7.244ProAla: 7.244 ± 0.782
0.39ProCys: 0.39 ± 0.138
4.736ProAsp: 4.736 ± 0.479
6.909ProGlu: 6.909 ± 0.882
1.059ProPhe: 1.059 ± 0.267
4.569ProGly: 4.569 ± 0.635
1.226ProHis: 1.226 ± 0.275
2.953ProIle: 2.953 ± 0.346
1.839ProLys: 1.839 ± 0.289
3.51ProLeu: 3.51 ± 0.492
1.17ProMet: 1.17 ± 0.239
2.507ProAsn: 2.507 ± 0.513
4.625ProPro: 4.625 ± 0.777
1.393ProGln: 1.393 ± 0.308
3.399ProArg: 3.399 ± 0.382
3.9ProSer: 3.9 ± 0.484
4.848ProThr: 4.848 ± 0.653
4.235ProVal: 4.235 ± 0.464
0.947ProTrp: 0.947 ± 0.249
1.504ProTyr: 1.504 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
5.238GlnAla: 5.238 ± 0.504
0.334GlnCys: 0.334 ± 0.195
1.393GlnAsp: 1.393 ± 0.257
1.282GlnGlu: 1.282 ± 0.294
0.557GlnPhe: 0.557 ± 0.166
2.396GlnGly: 2.396 ± 0.314
0.613GlnHis: 0.613 ± 0.196
1.839GlnIle: 1.839 ± 0.283
0.613GlnLys: 0.613 ± 0.183
0.892GlnLeu: 0.892 ± 0.386
0.836GlnMet: 0.836 ± 0.172
0.501GlnAsn: 0.501 ± 0.154
1.003GlnPro: 1.003 ± 0.311
0.724GlnGln: 0.724 ± 0.169
1.95GlnArg: 1.95 ± 0.334
1.839GlnSer: 1.839 ± 0.291
2.507GlnThr: 2.507 ± 0.401
2.229GlnVal: 2.229 ± 0.315
0.724GlnTrp: 0.724 ± 0.246
1.17GlnTyr: 1.17 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
9.361ArgAla: 9.361 ± 0.885
0.724ArgCys: 0.724 ± 0.239
4.29ArgAsp: 4.29 ± 0.657
5.461ArgGlu: 5.461 ± 0.745
1.95ArgPhe: 1.95 ± 0.329
5.962ArgGly: 5.962 ± 0.673
1.226ArgHis: 1.226 ± 0.25
3.009ArgIle: 3.009 ± 0.402
3.065ArgLys: 3.065 ± 0.616
5.628ArgLeu: 5.628 ± 0.52
2.117ArgMet: 2.117 ± 0.389
1.783ArgAsn: 1.783 ± 0.266
2.897ArgPro: 2.897 ± 0.467
2.619ArgGln: 2.619 ± 0.475
7.522ArgArg: 7.522 ± 0.78
3.287ArgSer: 3.287 ± 0.377
5.126ArgThr: 5.126 ± 0.622
4.848ArgVal: 4.848 ± 0.515
1.727ArgTrp: 1.727 ± 0.317
2.062ArgTyr: 2.062 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
6.408SerAla: 6.408 ± 0.827
0.223SerCys: 0.223 ± 0.104
2.619SerAsp: 2.619 ± 0.365
2.563SerGlu: 2.563 ± 0.353
1.393SerPhe: 1.393 ± 0.309
4.625SerGly: 4.625 ± 0.745
1.003SerHis: 1.003 ± 0.225
2.842SerIle: 2.842 ± 0.43
1.282SerLys: 1.282 ± 0.235
4.068SerLeu: 4.068 ± 0.574
1.003SerMet: 1.003 ± 0.245
1.003SerAsn: 1.003 ± 0.243
3.232SerPro: 3.232 ± 0.479
1.337SerGln: 1.337 ± 0.292
3.9SerArg: 3.9 ± 0.611
2.173SerSer: 2.173 ± 0.39
4.458SerThr: 4.458 ± 0.498
4.402SerVal: 4.402 ± 0.548
1.059SerTrp: 1.059 ± 0.304
0.892SerTyr: 0.892 ± 0.24
0.0SerXaa: 0.0 ± 0.0
Thr
8.971ThrAla: 8.971 ± 0.823
0.892ThrCys: 0.892 ± 0.238
4.903ThrAsp: 4.903 ± 0.483
3.845ThrGlu: 3.845 ± 0.55
1.839ThrPhe: 1.839 ± 0.36
7.299ThrGly: 7.299 ± 0.701
1.114ThrHis: 1.114 ± 0.243
5.126ThrIle: 5.126 ± 0.717
2.062ThrLys: 2.062 ± 0.337
5.182ThrLeu: 5.182 ± 0.502
1.616ThrMet: 1.616 ± 0.27
1.56ThrAsn: 1.56 ± 0.371
6.798ThrPro: 6.798 ± 0.781
2.062ThrGln: 2.062 ± 0.413
4.903ThrArg: 4.903 ± 0.615
3.176ThrSer: 3.176 ± 0.4
5.126ThrThr: 5.126 ± 0.607
5.516ThrVal: 5.516 ± 0.721
1.337ThrTrp: 1.337 ± 0.274
1.616ThrTyr: 1.616 ± 0.322
0.0ThrXaa: 0.0 ± 0.0
Val
8.637ValAla: 8.637 ± 0.756
0.557ValCys: 0.557 ± 0.22
3.845ValAsp: 3.845 ± 0.53
4.29ValGlu: 4.29 ± 0.432
1.226ValPhe: 1.226 ± 0.229
6.073ValGly: 6.073 ± 0.581
0.947ValHis: 0.947 ± 0.283
3.399ValIle: 3.399 ± 0.399
2.062ValLys: 2.062 ± 0.339
5.07ValLeu: 5.07 ± 0.416
1.504ValMet: 1.504 ± 0.328
1.616ValAsn: 1.616 ± 0.339
5.126ValPro: 5.126 ± 0.545
1.894ValGln: 1.894 ± 0.417
4.625ValArg: 4.625 ± 0.499
3.399ValSer: 3.399 ± 0.549
7.244ValThr: 7.244 ± 0.673
5.238ValVal: 5.238 ± 0.543
1.337ValTrp: 1.337 ± 0.27
1.839ValTyr: 1.839 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
2.173TrpAla: 2.173 ± 0.47
0.446TrpCys: 0.446 ± 0.155
0.78TrpAsp: 0.78 ± 0.165
1.616TrpGlu: 1.616 ± 0.305
0.501TrpPhe: 0.501 ± 0.191
1.393TrpGly: 1.393 ± 0.284
0.501TrpHis: 0.501 ± 0.154
0.613TrpIle: 0.613 ± 0.213
0.724TrpLys: 0.724 ± 0.228
1.56TrpLeu: 1.56 ± 0.302
0.446TrpMet: 0.446 ± 0.141
0.501TrpAsn: 0.501 ± 0.174
1.226TrpPro: 1.226 ± 0.259
1.17TrpGln: 1.17 ± 0.255
1.449TrpArg: 1.449 ± 0.253
1.504TrpSer: 1.504 ± 0.272
1.56TrpThr: 1.56 ± 0.288
1.337TrpVal: 1.337 ± 0.253
0.557TrpTrp: 0.557 ± 0.205
0.223TrpTyr: 0.223 ± 0.106
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.455TyrAla: 3.455 ± 0.506
0.223TyrCys: 0.223 ± 0.139
1.56TyrAsp: 1.56 ± 0.329
1.839TyrGlu: 1.839 ± 0.33
0.446TyrPhe: 0.446 ± 0.257
1.95TyrGly: 1.95 ± 0.328
0.279TyrHis: 0.279 ± 0.124
0.947TyrIle: 0.947 ± 0.227
0.446TyrLys: 0.446 ± 0.154
1.727TyrLeu: 1.727 ± 0.305
0.724TyrMet: 0.724 ± 0.2
0.557TyrAsn: 0.557 ± 0.205
0.892TyrPro: 0.892 ± 0.173
0.724TyrGln: 0.724 ± 0.179
2.006TyrArg: 2.006 ± 0.34
1.504TyrSer: 1.504 ± 0.269
1.059TyrThr: 1.059 ± 0.235
1.839TyrVal: 1.839 ± 0.348
0.39TyrTrp: 0.39 ± 0.147
0.557TyrTyr: 0.557 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17948 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski