Xanthomonas phage Bosa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pamexvirus; unclassified Pamexvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A679KER9|A0A679KER9_9CAUD Uncharacterized protein OS=Xanthomonas phage Bosa OX=2674976 PE=4 SV=1
MM1 pKa = 7.57AAYY4 pKa = 10.03SCNICGKK11 pKa = 10.25GFDD14 pKa = 5.49FGDD17 pKa = 4.69CSCDD21 pKa = 3.56SQPEE25 pKa = 4.11EE26 pKa = 4.45ASRR29 pKa = 11.84ALPTVEE35 pKa = 3.73QVAIAFVRR43 pKa = 11.84ILRR46 pKa = 11.84EE47 pKa = 4.01TVGEE51 pKa = 3.8EE52 pKa = 4.06DD53 pKa = 3.68YY54 pKa = 11.84AKK56 pKa = 10.31IVALNAAEE64 pKa = 4.71LNPDD68 pKa = 3.27VCNSGDD74 pKa = 3.61FCDD77 pKa = 4.56SNMVMDD83 pKa = 5.15AALKK87 pKa = 10.51EE88 pKa = 4.39FGVDD92 pKa = 3.49PAPEE96 pKa = 4.09EE97 pKa = 5.01DD98 pKa = 4.41DD99 pKa = 5.76FEE101 pKa = 4.7MPDD104 pKa = 3.77DD105 pKa = 4.86VHH107 pKa = 7.38VLWNAAWDD115 pKa = 3.87RR116 pKa = 11.84AKK118 pKa = 11.1EE119 pKa = 3.92LMQQASS125 pKa = 3.32

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A679KLM4|A0A679KLM4_9CAUD Phage protein OS=Xanthomonas phage Bosa OX=2674976 PE=4 SV=1
MM1 pKa = 7.87PNTARR6 pKa = 11.84TIKK9 pKa = 10.64EE10 pKa = 3.76EE11 pKa = 3.66VRR13 pKa = 11.84ILGRR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.68RR20 pKa = 11.84ALTYY24 pKa = 9.93PAMVDD29 pKa = 3.54IIKK32 pKa = 10.42ARR34 pKa = 11.84HH35 pKa = 5.83PEE37 pKa = 3.89ANTSVKK43 pKa = 8.91TVQWYY48 pKa = 10.09ASRR51 pKa = 11.84LRR53 pKa = 11.84RR54 pKa = 11.84DD55 pKa = 3.63GEE57 pKa = 4.22DD58 pKa = 3.26VNVKK62 pKa = 10.35DD63 pKa = 4.94GRR65 pKa = 11.84TAPGKK70 pKa = 8.28PTVHH74 pKa = 6.78

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

19317

50

1189

241.5

26.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.359 ± 0.424

0.885 ± 0.12

6.492 ± 0.245

6.492 ± 0.293

3.448 ± 0.202

8.278 ± 0.297

1.884 ± 0.188

3.774 ± 0.169

4.939 ± 0.355

8.64 ± 0.162

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.148

3.163 ± 0.11

5.239 ± 0.248

4.079 ± 0.197

6.347 ± 0.282

5.964 ± 0.277

5.767 ± 0.298

6.963 ± 0.179

1.869 ± 0.15

2.827 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski