Amino acid dipepetide frequency for Proteus phage Privateer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.29AlaAla: 3.29 ± 0.539
0.63AlaCys: 0.63 ± 0.154
2.52AlaAsp: 2.52 ± 0.359
3.92AlaGlu: 3.92 ± 0.586
2.31AlaPhe: 2.31 ± 0.317
3.85AlaGly: 3.85 ± 0.531
0.98AlaHis: 0.98 ± 0.195
3.78AlaIle: 3.78 ± 0.421
3.5AlaLys: 3.5 ± 0.316
4.2AlaLeu: 4.2 ± 0.409
1.54AlaMet: 1.54 ± 0.227
3.08AlaAsn: 3.08 ± 0.298
1.96AlaPro: 1.96 ± 0.255
2.52AlaGln: 2.52 ± 0.353
2.8AlaArg: 2.8 ± 0.332
3.92AlaSer: 3.92 ± 0.414
3.22AlaThr: 3.22 ± 0.433
3.745AlaVal: 3.745 ± 0.371
0.35AlaTrp: 0.35 ± 0.112
1.82AlaTyr: 1.82 ± 0.251
0.0AlaXaa: 0.0 ± 0.0
Cys
0.595CysAla: 0.595 ± 0.168
0.245CysCys: 0.245 ± 0.102
0.805CysAsp: 0.805 ± 0.19
0.91CysGlu: 0.91 ± 0.193
0.595CysPhe: 0.595 ± 0.163
1.155CysGly: 1.155 ± 0.268
0.28CysHis: 0.28 ± 0.112
0.77CysIle: 0.77 ± 0.174
0.735CysLys: 0.735 ± 0.175
0.875CysLeu: 0.875 ± 0.168
0.315CysMet: 0.315 ± 0.127
0.63CysAsn: 0.63 ± 0.138
0.525CysPro: 0.525 ± 0.152
0.385CysGln: 0.385 ± 0.113
0.385CysArg: 0.385 ± 0.106
0.875CysSer: 0.875 ± 0.197
0.63CysThr: 0.63 ± 0.138
0.63CysVal: 0.63 ± 0.133
0.105CysTrp: 0.105 ± 0.066
0.49CysTyr: 0.49 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
3.605AspAla: 3.605 ± 0.508
0.875AspCys: 0.875 ± 0.196
3.955AspAsp: 3.955 ± 0.44
4.305AspGlu: 4.305 ± 0.466
2.59AspPhe: 2.59 ± 0.393
5.18AspGly: 5.18 ± 0.413
1.225AspHis: 1.225 ± 0.224
5.215AspIle: 5.215 ± 0.498
5.04AspLys: 5.04 ± 0.522
5.95AspLeu: 5.95 ± 0.512
1.89AspMet: 1.89 ± 0.253
4.48AspAsn: 4.48 ± 0.377
2.38AspPro: 2.38 ± 0.291
1.855AspGln: 1.855 ± 0.312
3.01AspArg: 3.01 ± 0.324
5.25AspSer: 5.25 ± 0.498
3.85AspThr: 3.85 ± 0.419
3.885AspVal: 3.885 ± 0.295
1.015AspTrp: 1.015 ± 0.195
2.835AspTyr: 2.835 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
4.655GluAla: 4.655 ± 0.467
0.945GluCys: 0.945 ± 0.213
4.865GluAsp: 4.865 ± 0.519
6.615GluGlu: 6.615 ± 0.593
2.87GluPhe: 2.87 ± 0.27
3.955GluGly: 3.955 ± 0.397
1.085GluHis: 1.085 ± 0.162
4.48GluIle: 4.48 ± 0.401
4.935GluLys: 4.935 ± 0.499
6.09GluLeu: 6.09 ± 0.552
2.73GluMet: 2.73 ± 0.256
4.095GluAsn: 4.095 ± 0.437
1.96GluPro: 1.96 ± 0.351
2.345GluGln: 2.345 ± 0.327
3.185GluArg: 3.185 ± 0.408
4.76GluSer: 4.76 ± 0.682
3.57GluThr: 3.57 ± 0.301
5.075GluVal: 5.075 ± 0.492
0.735GluTrp: 0.735 ± 0.136
2.905GluTyr: 2.905 ± 0.34
0.0GluXaa: 0.0 ± 0.0
Phe
1.61PheAla: 1.61 ± 0.256
0.525PheCys: 0.525 ± 0.161
3.99PheAsp: 3.99 ± 0.456
2.73PheGlu: 2.73 ± 0.302
1.61PhePhe: 1.61 ± 0.267
2.905PheGly: 2.905 ± 0.391
0.91PheHis: 0.91 ± 0.175
2.94PheIle: 2.94 ± 0.422
4.025PheLys: 4.025 ± 0.463
2.94PheLeu: 2.94 ± 0.372
1.225PheMet: 1.225 ± 0.215
3.22PheAsn: 3.22 ± 0.364
1.26PhePro: 1.26 ± 0.227
1.33PheGln: 1.33 ± 0.201
1.575PheArg: 1.575 ± 0.255
3.43PheSer: 3.43 ± 0.326
2.135PheThr: 2.135 ± 0.305
2.87PheVal: 2.87 ± 0.377
0.35PheTrp: 0.35 ± 0.117
1.82PheTyr: 1.82 ± 0.319
0.0PheXaa: 0.0 ± 0.0
Gly
3.815GlyAla: 3.815 ± 0.461
0.735GlyCys: 0.735 ± 0.201
4.445GlyAsp: 4.445 ± 0.421
4.55GlyGlu: 4.55 ± 0.371
3.185GlyPhe: 3.185 ± 0.328
4.585GlyGly: 4.585 ± 0.659
1.015GlyHis: 1.015 ± 0.196
4.13GlyIle: 4.13 ± 0.414
5.705GlyLys: 5.705 ± 0.517
5.075GlyLeu: 5.075 ± 0.42
2.205GlyMet: 2.205 ± 0.312
3.99GlyAsn: 3.99 ± 0.478
0.56GlyPro: 0.56 ± 0.162
1.925GlyGln: 1.925 ± 0.301
2.59GlyArg: 2.59 ± 0.306
4.865GlySer: 4.865 ± 0.545
3.64GlyThr: 3.64 ± 0.35
4.9GlyVal: 4.9 ± 0.492
1.26GlyTrp: 1.26 ± 0.227
2.8GlyTyr: 2.8 ± 0.327
0.0GlyXaa: 0.0 ± 0.0
His
0.665HisAla: 0.665 ± 0.202
0.455HisCys: 0.455 ± 0.119
1.05HisAsp: 1.05 ± 0.156
1.05HisGlu: 1.05 ± 0.186
0.875HisPhe: 0.875 ± 0.138
1.05HisGly: 1.05 ± 0.196
0.175HisHis: 0.175 ± 0.089
1.225HisIle: 1.225 ± 0.211
1.26HisLys: 1.26 ± 0.226
1.575HisLeu: 1.575 ± 0.19
0.385HisMet: 0.385 ± 0.104
1.085HisAsn: 1.085 ± 0.195
0.84HisPro: 0.84 ± 0.155
0.455HisGln: 0.455 ± 0.126
1.435HisArg: 1.435 ± 0.211
1.54HisSer: 1.54 ± 0.278
0.805HisThr: 0.805 ± 0.222
0.98HisVal: 0.98 ± 0.219
0.175HisTrp: 0.175 ± 0.075
0.84HisTyr: 0.84 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
3.43IleAla: 3.43 ± 0.304
0.77IleCys: 0.77 ± 0.176
5.32IleAsp: 5.32 ± 0.461
4.865IleGlu: 4.865 ± 0.469
2.73IlePhe: 2.73 ± 0.352
4.025IleGly: 4.025 ± 0.459
1.4IleHis: 1.4 ± 0.273
4.41IleIle: 4.41 ± 0.386
5.845IleLys: 5.845 ± 0.523
5.075IleLeu: 5.075 ± 0.46
1.68IleMet: 1.68 ± 0.252
4.515IleAsn: 4.515 ± 0.375
3.29IlePro: 3.29 ± 0.336
1.855IleGln: 1.855 ± 0.287
3.325IleArg: 3.325 ± 0.402
4.655IleSer: 4.655 ± 0.453
4.41IleThr: 4.41 ± 0.481
3.64IleVal: 3.64 ± 0.407
0.455IleTrp: 0.455 ± 0.114
2.695IleTyr: 2.695 ± 0.331
0.0IleXaa: 0.0 ± 0.0
Lys
4.76LysAla: 4.76 ± 0.623
0.42LysCys: 0.42 ± 0.151
6.755LysAsp: 6.755 ± 0.56
6.895LysGlu: 6.895 ± 0.712
2.45LysPhe: 2.45 ± 0.311
4.97LysGly: 4.97 ± 0.425
1.295LysHis: 1.295 ± 0.178
5.11LysIle: 5.11 ± 0.42
6.755LysLys: 6.755 ± 0.542
6.51LysLeu: 6.51 ± 0.517
2.59LysMet: 2.59 ± 0.343
3.885LysAsn: 3.885 ± 0.378
3.325LysPro: 3.325 ± 0.432
2.345LysGln: 2.345 ± 0.303
3.43LysArg: 3.43 ± 0.349
4.34LysSer: 4.34 ± 0.326
4.76LysThr: 4.76 ± 0.42
4.865LysVal: 4.865 ± 0.383
1.225LysTrp: 1.225 ± 0.203
3.43LysTyr: 3.43 ± 0.414
0.0LysXaa: 0.0 ± 0.0
Leu
4.655LeuAla: 4.655 ± 0.513
1.12LeuCys: 1.12 ± 0.27
6.23LeuAsp: 6.23 ± 0.401
4.83LeuGlu: 4.83 ± 0.446
3.01LeuPhe: 3.01 ± 0.35
4.795LeuGly: 4.795 ± 0.488
1.33LeuHis: 1.33 ± 0.217
5.635LeuIle: 5.635 ± 0.455
5.915LeuLys: 5.915 ± 0.655
5.81LeuLeu: 5.81 ± 0.595
2.17LeuMet: 2.17 ± 0.254
4.375LeuAsn: 4.375 ± 0.427
2.905LeuPro: 2.905 ± 0.276
2.765LeuGln: 2.765 ± 0.373
3.745LeuArg: 3.745 ± 0.402
7.245LeuSer: 7.245 ± 0.46
4.165LeuThr: 4.165 ± 0.363
4.445LeuVal: 4.445 ± 0.439
0.56LeuTrp: 0.56 ± 0.146
3.08LeuTyr: 3.08 ± 0.336
0.0LeuXaa: 0.0 ± 0.0
Met
1.995MetAla: 1.995 ± 0.272
0.42MetCys: 0.42 ± 0.133
1.645MetAsp: 1.645 ± 0.213
1.925MetGlu: 1.925 ± 0.256
1.75MetPhe: 1.75 ± 0.273
1.855MetGly: 1.855 ± 0.376
0.42MetHis: 0.42 ± 0.117
1.855MetIle: 1.855 ± 0.288
3.185MetLys: 3.185 ± 0.329
2.1MetLeu: 2.1 ± 0.315
1.26MetMet: 1.26 ± 0.202
1.89MetAsn: 1.89 ± 0.25
1.015MetPro: 1.015 ± 0.188
0.665MetGln: 0.665 ± 0.151
1.26MetArg: 1.26 ± 0.229
2.38MetSer: 2.38 ± 0.255
1.89MetThr: 1.89 ± 0.271
1.575MetVal: 1.575 ± 0.28
0.315MetTrp: 0.315 ± 0.094
0.77MetTyr: 0.77 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.395AsnAla: 3.395 ± 0.404
0.735AsnCys: 0.735 ± 0.175
3.045AsnAsp: 3.045 ± 0.286
4.13AsnGlu: 4.13 ± 0.367
2.485AsnPhe: 2.485 ± 0.303
4.165AsnGly: 4.165 ± 0.374
1.085AsnHis: 1.085 ± 0.241
4.48AsnIle: 4.48 ± 0.402
5.845AsnLys: 5.845 ± 0.433
4.655AsnLeu: 4.655 ± 0.366
1.75AsnMet: 1.75 ± 0.247
3.85AsnAsn: 3.85 ± 0.366
2.415AsnPro: 2.415 ± 0.253
2.31AsnGln: 2.31 ± 0.297
3.08AsnArg: 3.08 ± 0.293
4.165AsnSer: 4.165 ± 0.42
2.905AsnThr: 2.905 ± 0.266
2.73AsnVal: 2.73 ± 0.281
0.805AsnTrp: 0.805 ± 0.192
1.89AsnTyr: 1.89 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
1.33ProAla: 1.33 ± 0.221
0.525ProCys: 0.525 ± 0.131
2.59ProAsp: 2.59 ± 0.292
3.08ProGlu: 3.08 ± 0.381
2.31ProPhe: 2.31 ± 0.346
1.33ProGly: 1.33 ± 0.22
0.63ProHis: 0.63 ± 0.161
2.275ProIle: 2.275 ± 0.278
2.52ProLys: 2.52 ± 0.409
1.89ProLeu: 1.89 ± 0.254
1.085ProMet: 1.085 ± 0.197
1.82ProAsn: 1.82 ± 0.291
0.91ProPro: 0.91 ± 0.161
1.225ProGln: 1.225 ± 0.228
0.945ProArg: 0.945 ± 0.162
2.66ProSer: 2.66 ± 0.386
2.03ProThr: 2.03 ± 0.244
2.66ProVal: 2.66 ± 0.375
0.42ProTrp: 0.42 ± 0.105
1.575ProTyr: 1.575 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
1.925GlnAla: 1.925 ± 0.263
0.14GlnCys: 0.14 ± 0.067
1.995GlnAsp: 1.995 ± 0.301
2.45GlnGlu: 2.45 ± 0.334
1.33GlnPhe: 1.33 ± 0.253
1.96GlnGly: 1.96 ± 0.368
0.385GlnHis: 0.385 ± 0.112
2.24GlnIle: 2.24 ± 0.308
2.17GlnLys: 2.17 ± 0.263
2.555GlnLeu: 2.555 ± 0.337
1.26GlnMet: 1.26 ± 0.237
2.065GlnAsn: 2.065 ± 0.36
1.12GlnPro: 1.12 ± 0.244
0.945GlnGln: 0.945 ± 0.257
1.4GlnArg: 1.4 ± 0.193
1.785GlnSer: 1.785 ± 0.268
1.715GlnThr: 1.715 ± 0.3
2.275GlnVal: 2.275 ± 0.336
0.42GlnTrp: 0.42 ± 0.138
1.05GlnTyr: 1.05 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
2.625ArgAla: 2.625 ± 0.276
0.455ArgCys: 0.455 ± 0.138
3.22ArgAsp: 3.22 ± 0.351
2.765ArgGlu: 2.765 ± 0.295
2.415ArgPhe: 2.415 ± 0.329
2.625ArgGly: 2.625 ± 0.372
0.875ArgHis: 0.875 ± 0.151
2.975ArgIle: 2.975 ± 0.331
3.815ArgLys: 3.815 ± 0.418
3.675ArgLeu: 3.675 ± 0.371
1.61ArgMet: 1.61 ± 0.245
2.94ArgAsn: 2.94 ± 0.349
1.295ArgPro: 1.295 ± 0.218
0.945ArgGln: 0.945 ± 0.227
2.59ArgArg: 2.59 ± 0.398
2.975ArgSer: 2.975 ± 0.342
2.135ArgThr: 2.135 ± 0.276
2.66ArgVal: 2.66 ± 0.299
0.56ArgTrp: 0.56 ± 0.179
1.575ArgTyr: 1.575 ± 0.245
0.0ArgXaa: 0.0 ± 0.0
Ser
3.5SerAla: 3.5 ± 0.435
0.98SerCys: 0.98 ± 0.196
4.445SerAsp: 4.445 ± 0.435
4.62SerGlu: 4.62 ± 0.443
3.92SerPhe: 3.92 ± 0.459
5.46SerGly: 5.46 ± 0.511
1.47SerHis: 1.47 ± 0.27
5.04SerIle: 5.04 ± 0.402
6.195SerLys: 6.195 ± 0.676
5.46SerLeu: 5.46 ± 0.489
1.715SerMet: 1.715 ± 0.216
4.095SerAsn: 4.095 ± 0.377
2.345SerPro: 2.345 ± 0.307
2.24SerGln: 2.24 ± 0.316
2.94SerArg: 2.94 ± 0.347
4.585SerSer: 4.585 ± 0.477
4.235SerThr: 4.235 ± 0.422
5.425SerVal: 5.425 ± 0.513
0.525SerTrp: 0.525 ± 0.131
2.625SerTyr: 2.625 ± 0.315
0.0SerXaa: 0.0 ± 0.0
Thr
2.975ThrAla: 2.975 ± 0.38
0.525ThrCys: 0.525 ± 0.17
3.22ThrAsp: 3.22 ± 0.441
3.29ThrGlu: 3.29 ± 0.278
2.38ThrPhe: 2.38 ± 0.279
4.76ThrGly: 4.76 ± 0.394
1.05ThrHis: 1.05 ± 0.166
3.92ThrIle: 3.92 ± 0.486
3.64ThrLys: 3.64 ± 0.4
5.95ThrLeu: 5.95 ± 0.463
1.085ThrMet: 1.085 ± 0.171
3.01ThrAsn: 3.01 ± 0.341
2.24ThrPro: 2.24 ± 0.286
1.75ThrGln: 1.75 ± 0.252
2.065ThrArg: 2.065 ± 0.257
3.85ThrSer: 3.85 ± 0.383
2.73ThrThr: 2.73 ± 0.338
3.675ThrVal: 3.675 ± 0.408
0.665ThrTrp: 0.665 ± 0.199
2.135ThrTyr: 2.135 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
2.66ValAla: 2.66 ± 0.304
0.805ValCys: 0.805 ± 0.21
4.445ValAsp: 4.445 ± 0.39
5.18ValGlu: 5.18 ± 0.464
2.415ValPhe: 2.415 ± 0.341
3.955ValGly: 3.955 ± 0.373
1.225ValHis: 1.225 ± 0.18
4.445ValIle: 4.445 ± 0.449
4.83ValLys: 4.83 ± 0.424
4.585ValLeu: 4.585 ± 0.425
1.995ValMet: 1.995 ± 0.276
4.06ValAsn: 4.06 ± 0.47
2.03ValPro: 2.03 ± 0.263
1.96ValGln: 1.96 ± 0.296
2.975ValArg: 2.975 ± 0.336
4.97ValSer: 4.97 ± 0.458
3.85ValThr: 3.85 ± 0.34
3.99ValVal: 3.99 ± 0.345
0.63ValTrp: 0.63 ± 0.143
2.205ValTyr: 2.205 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
0.56TrpAla: 0.56 ± 0.126
0.14TrpCys: 0.14 ± 0.075
0.735TrpAsp: 0.735 ± 0.204
1.085TrpGlu: 1.085 ± 0.212
0.665TrpPhe: 0.665 ± 0.174
0.945TrpGly: 0.945 ± 0.168
0.245TrpHis: 0.245 ± 0.087
0.945TrpIle: 0.945 ± 0.195
0.735TrpLys: 0.735 ± 0.181
0.91TrpLeu: 0.91 ± 0.198
0.35TrpMet: 0.35 ± 0.113
0.665TrpAsn: 0.665 ± 0.161
0.105TrpPro: 0.105 ± 0.064
0.21TrpGln: 0.21 ± 0.09
0.42TrpArg: 0.42 ± 0.152
1.015TrpSer: 1.015 ± 0.261
0.455TrpThr: 0.455 ± 0.131
0.7TrpVal: 0.7 ± 0.14
0.245TrpTrp: 0.245 ± 0.106
0.175TrpTyr: 0.175 ± 0.078
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.89TyrAla: 1.89 ± 0.228
0.49TyrCys: 0.49 ± 0.165
3.115TyrAsp: 3.115 ± 0.391
2.66TyrGlu: 2.66 ± 0.287
1.505TyrPhe: 1.505 ± 0.247
2.45TyrGly: 2.45 ± 0.272
0.84TyrHis: 0.84 ± 0.193
2.52TyrIle: 2.52 ± 0.309
3.465TyrLys: 3.465 ± 0.376
2.905TyrLeu: 2.905 ± 0.315
1.295TyrMet: 1.295 ± 0.182
2.17TyrAsn: 2.17 ± 0.197
1.33TyrPro: 1.33 ± 0.26
1.155TyrGln: 1.155 ± 0.2
1.61TyrArg: 1.61 ± 0.251
2.555TyrSer: 2.555 ± 0.296
1.785TyrThr: 1.785 ± 0.258
2.485TyrVal: 2.485 ± 0.285
0.49TyrTrp: 0.49 ± 0.15
1.54TyrTyr: 1.54 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 144 proteins (28573 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski