Streptomyces sp. TverLS-915

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C4NDN9|A0A1C4NDN9_9ACTN Transcriptional regulator TetR family OS=Streptomyces sp. TverLS-915 OX=1839763 GN=GA0115251_131118 PE=4 SV=1
MM1 pKa = 7.62SNPTNLRR8 pKa = 11.84YY9 pKa = 10.16SKK11 pKa = 9.51EE12 pKa = 4.21HH13 pKa = 5.94EE14 pKa = 4.35WLSAAADD21 pKa = 3.98GVSTVGITAYY31 pKa = 10.47AAGQLGDD38 pKa = 3.59VVYY41 pKa = 10.63AQLPEE46 pKa = 4.24VGDD49 pKa = 3.85SVEE52 pKa = 4.29AGEE55 pKa = 4.55TCGEE59 pKa = 4.12LEE61 pKa = 4.31STKK64 pKa = 10.61SVSDD68 pKa = 4.01LYY70 pKa = 11.51SPVNGEE76 pKa = 3.99VTEE79 pKa = 4.31VNSDD83 pKa = 3.63VVEE86 pKa = 4.72DD87 pKa = 4.11PSLVNSAPFEE97 pKa = 4.42GGWLFKK103 pKa = 11.02VRR105 pKa = 11.84VSEE108 pKa = 4.21EE109 pKa = 3.99PGDD112 pKa = 3.81LLSAEE117 pKa = 4.62EE118 pKa = 4.07YY119 pKa = 10.95AAFAGSDD126 pKa = 3.58DD127 pKa = 3.64

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C4K5M3|A0A1C4K5M3_9ACTN Putative efflux protein MATE family OS=Streptomyces sp. TverLS-915 OX=1839763 GN=GA0115251_112542 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.46GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6283

0

6283

2005380

24

6338

319.2

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.231 ± 0.05

0.729 ± 0.008

5.703 ± 0.026

5.862 ± 0.037

2.641 ± 0.015

9.909 ± 0.034

2.321 ± 0.017

2.664 ± 0.019

2.11 ± 0.03

10.547 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.532 ± 0.012

1.614 ± 0.017

6.568 ± 0.032

2.407 ± 0.018

8.364 ± 0.04

4.862 ± 0.022

6.097 ± 0.029

8.306 ± 0.032

1.487 ± 0.012

2.045 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski