Streptococcus satellite phage Javan734

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZVE4|A0A4D5ZVE4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan734 OX=2558828 GN=JavanS734_0019 PE=4 SV=1
MM1 pKa = 7.22TLDD4 pKa = 4.01LGKK7 pKa = 8.39MTQAEE12 pKa = 4.45FDD14 pKa = 3.72EE15 pKa = 4.66VMADD19 pKa = 3.21IKK21 pKa = 11.13ARR23 pKa = 11.84NPNLLQFITDD33 pKa = 3.81LLDD36 pKa = 3.75RR37 pKa = 11.84KK38 pKa = 8.57VTPEE42 pKa = 4.1EE43 pKa = 4.0VDD45 pKa = 3.88DD46 pKa = 4.43LLNMEE51 pKa = 4.04QAEE54 pKa = 4.0QVEE57 pKa = 4.75YY58 pKa = 10.8IEE60 pKa = 5.26NYY62 pKa = 7.17QAKK65 pKa = 10.08AA66 pKa = 3.19

Molecular weight:
7.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZVD4|A0A4D5ZVD4_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan734 OX=2558828 GN=JavanS734_0009 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84CEE4 pKa = 4.37MMPKK8 pKa = 9.9SNYY11 pKa = 9.57RR12 pKa = 11.84EE13 pKa = 3.74MRR15 pKa = 11.84FISSLGGKK23 pKa = 9.41LLATRR28 pKa = 11.84ILFTVTIANSQQ39 pKa = 3.1

Molecular weight:
4.5 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3069

39

507

139.5

16.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.442 ± 0.46

0.978 ± 0.209

5.409 ± 0.364

7.983 ± 0.556

4.301 ± 0.54

4.725 ± 0.532

1.857 ± 0.27

7.429 ± 0.423

9.677 ± 0.608

10.231 ± 0.604

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.426

5.767 ± 0.405

3.128 ± 0.4

3.975 ± 0.336

4.888 ± 0.383

5.539 ± 0.491

5.474 ± 0.49

5.213 ± 0.399

0.912 ± 0.19

4.431 ± 0.35

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski