Amino acid dipepetide frequency for Rhodobacteraceae bacterium 2CG4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.583AlaAla: 22.583 ± 0.234	1.213AlaCys: 1.213 ± 0.034	7.929AlaAsp: 7.929 ± 0.09	8.913AlaGlu: 8.913 ± 0.097	4.5AlaPhe: 4.5 ± 0.056	12.833AlaGly: 12.833 ± 0.116	2.627AlaHis: 2.627 ± 0.046	5.686AlaIle: 5.686 ± 0.076	2.564AlaLys: 2.564 ± 0.052	15.926AlaLeu: 15.926 ± 0.158	3.739AlaMet: 3.739 ± 0.051	2.444AlaAsn: 2.444 ± 0.042	7.21AlaPro: 7.21 ± 0.101	4.635AlaGln: 4.635 ± 0.056	11.665AlaArg: 11.665 ± 0.131	5.365AlaSer: 5.365 ± 0.065	5.976AlaThr: 5.976 ± 0.062	9.552AlaVal: 9.552 ± 0.093	1.724AlaTrp: 1.724 ± 0.041	2.446AlaTyr: 2.446 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.174CysAla: 1.174 ± 0.032	0.134CysCys: 0.134 ± 0.01	0.589CysAsp: 0.589 ± 0.022	0.453CysGlu: 0.453 ± 0.019	0.325CysPhe: 0.325 ± 0.016	0.998CysGly: 0.998 ± 0.031	0.261CysHis: 0.261 ± 0.015	0.351CysIle: 0.351 ± 0.017	0.14CysLys: 0.14 ± 0.01	0.803CysLeu: 0.803 ± 0.026	0.168CysMet: 0.168 ± 0.01	0.203CysAsn: 0.203 ± 0.013	0.536CysPro: 0.536 ± 0.021	0.217CysGln: 0.217 ± 0.015	0.752CysArg: 0.752 ± 0.025	0.405CysSer: 0.405 ± 0.018	0.457CysThr: 0.457 ± 0.019	0.634CysVal: 0.634 ± 0.022	0.121CysTrp: 0.121 ± 0.009	0.182CysTyr: 0.182 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.308AspAla: 8.308 ± 0.096	0.525AspCys: 0.525 ± 0.02	3.331AspAsp: 3.331 ± 0.055	3.446AspGlu: 3.446 ± 0.06	2.089AspPhe: 2.089 ± 0.038	6.12AspGly: 6.12 ± 0.083	1.256AspHis: 1.256 ± 0.037	2.75AspIle: 2.75 ± 0.042	1.287AspLys: 1.287 ± 0.039	6.21AspLeu: 6.21 ± 0.065	1.46AspMet: 1.46 ± 0.034	1.109AspAsn: 1.109 ± 0.03	4.207AspPro: 4.207 ± 0.063	1.56AspGln: 1.56 ± 0.033	5.23AspArg: 5.23 ± 0.063	2.148AspSer: 2.148 ± 0.035	2.754AspThr: 2.754 ± 0.052	4.01AspVal: 4.01 ± 0.059	1.207AspTrp: 1.207 ± 0.033	1.379AspTyr: 1.379 ± 0.032	0.001AspXaa: 0.001 ± 0.001
Glu	8.151GluAla: 8.151 ± 0.091	0.351GluCys: 0.351 ± 0.018	3.11GluAsp: 3.11 ± 0.048	2.799GluGlu: 2.799 ± 0.059	1.759GluPhe: 1.759 ± 0.035	4.319GluGly: 4.319 ± 0.061	1.207GluHis: 1.207 ± 0.031	3.226GluIle: 3.226 ± 0.057	1.585GluLys: 1.585 ± 0.044	5.151GluLeu: 5.151 ± 0.076	1.598GluMet: 1.598 ± 0.034	1.36GluAsn: 1.36 ± 0.035	2.765GluPro: 2.765 ± 0.053	1.948GluGln: 1.948 ± 0.036	4.731GluArg: 4.731 ± 0.071	2.071GluSer: 2.071 ± 0.045	3.513GluThr: 3.513 ± 0.052	4.239GluVal: 4.239 ± 0.06	0.669GluTrp: 0.669 ± 0.023	0.975GluTyr: 0.975 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.611PheAla: 4.611 ± 0.076	0.379PheCys: 0.379 ± 0.016	2.712PheAsp: 2.712 ± 0.049	1.969PheGlu: 1.969 ± 0.037	1.311PhePhe: 1.311 ± 0.034	3.708PheGly: 3.708 ± 0.062	0.73PheHis: 0.73 ± 0.027	1.384PheIle: 1.384 ± 0.036	0.652PheLys: 0.652 ± 0.023	3.393PheLeu: 3.393 ± 0.055	0.762PheMet: 0.762 ± 0.022	0.842PheAsn: 0.842 ± 0.028	1.633PhePro: 1.633 ± 0.036	0.906PheGln: 0.906 ± 0.021	2.43PheArg: 2.43 ± 0.044	1.799PheSer: 1.799 ± 0.035	1.838PheThr: 1.838 ± 0.04	2.793PheVal: 2.793 ± 0.055	0.55PheTrp: 0.55 ± 0.019	0.847PheTyr: 0.847 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	11.8GlyAla: 11.8 ± 0.111	0.945GlyCys: 0.945 ± 0.032	4.944GlyAsp: 4.944 ± 0.058	4.534GlyGlu: 4.534 ± 0.063	3.646GlyPhe: 3.646 ± 0.053	8.236GlyGly: 8.236 ± 0.118	1.973GlyHis: 1.973 ± 0.044	4.417GlyIle: 4.417 ± 0.06	2.476GlyLys: 2.476 ± 0.052	9.634GlyLeu: 9.634 ± 0.099	2.561GlyMet: 2.561 ± 0.044	1.934GlyAsn: 1.934 ± 0.045	4.302GlyPro: 4.302 ± 0.056	2.949GlyGln: 2.949 ± 0.05	7.34GlyArg: 7.34 ± 0.081	4.1GlySer: 4.1 ± 0.055	4.72GlyThr: 4.72 ± 0.058	6.607GlyVal: 6.607 ± 0.071	1.579GlyTrp: 1.579 ± 0.032	2.223GlyTyr: 2.223 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.67HisAla: 2.67 ± 0.047	0.247HisCys: 0.247 ± 0.012	1.324HisAsp: 1.324 ± 0.035	1.055HisGlu: 1.055 ± 0.03	0.758HisPhe: 0.758 ± 0.026	2.046HisGly: 2.046 ± 0.042	0.546HisHis: 0.546 ± 0.023	0.812HisIle: 0.812 ± 0.026	0.397HisLys: 0.397 ± 0.017	2.102HisLeu: 2.102 ± 0.039	0.516HisMet: 0.516 ± 0.019	0.385HisAsn: 0.385 ± 0.018	1.442HisPro: 1.442 ± 0.036	0.546HisGln: 0.546 ± 0.02	1.68HisArg: 1.68 ± 0.042	0.795HisSer: 0.795 ± 0.023	0.748HisThr: 0.748 ± 0.024	1.522HisVal: 1.522 ± 0.035	0.364HisTrp: 0.364 ± 0.016	0.524HisTyr: 0.524 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	7.013IleAla: 7.013 ± 0.071	0.544IleCys: 0.544 ± 0.02	3.358IleAsp: 3.358 ± 0.051	3.075IleGlu: 3.075 ± 0.05	1.549IlePhe: 1.549 ± 0.038	4.683IleGly: 4.683 ± 0.072	0.771IleHis: 0.771 ± 0.026	1.707IleIle: 1.707 ± 0.047	0.865IleLys: 0.865 ± 0.027	4.346IleLeu: 4.346 ± 0.074	0.868IleMet: 0.868 ± 0.026	1.044IleAsn: 1.044 ± 0.027	2.22IlePro: 2.22 ± 0.041	0.968IleGln: 0.968 ± 0.026	3.339IleArg: 3.339 ± 0.053	2.351IleSer: 2.351 ± 0.051	2.304IleThr: 2.304 ± 0.043	3.66IleVal: 3.66 ± 0.057	0.615IleTrp: 0.615 ± 0.021	1.041IleTyr: 1.041 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.967LysAla: 2.967 ± 0.049	0.135LysCys: 0.135 ± 0.011	1.235LysAsp: 1.235 ± 0.035	1.003LysGlu: 1.003 ± 0.032	0.651LysPhe: 0.651 ± 0.02	1.934LysGly: 1.934 ± 0.042	0.494LysHis: 0.494 ± 0.021	1.235LysIle: 1.235 ± 0.032	0.735LysLys: 0.735 ± 0.027	2.304LysLeu: 2.304 ± 0.047	0.62LysMet: 0.62 ± 0.023	0.533LysAsn: 0.533 ± 0.02	1.461LysPro: 1.461 ± 0.034	0.673LysGln: 0.673 ± 0.026	1.746LysArg: 1.746 ± 0.044	1.251LysSer: 1.251 ± 0.04	1.37LysThr: 1.37 ± 0.032	1.767LysVal: 1.767 ± 0.043	0.285LysTrp: 0.285 ± 0.014	0.482LysTyr: 0.482 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	15.133LeuAla: 15.133 ± 0.167	0.899LeuCys: 0.899 ± 0.029	6.589LeuAsp: 6.589 ± 0.079	5.384LeuGlu: 5.384 ± 0.063	3.544LeuPhe: 3.544 ± 0.069	8.915LeuGly: 8.915 ± 0.096	2.005LeuHis: 2.005 ± 0.038	4.681LeuIle: 4.681 ± 0.07	2.6LeuLys: 2.6 ± 0.048	9.86LeuLeu: 9.86 ± 0.118	2.339LeuMet: 2.339 ± 0.048	2.346LeuAsn: 2.346 ± 0.041	6.0LeuPro: 6.0 ± 0.076	2.611LeuGln: 2.611 ± 0.039	8.07LeuArg: 8.07 ± 0.089	6.081LeuSer: 6.081 ± 0.079	5.803LeuThr: 5.803 ± 0.074	7.309LeuVal: 7.309 ± 0.092	1.315LeuTrp: 1.315 ± 0.034	1.945LeuTyr: 1.945 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	3.236MetAla: 3.236 ± 0.053	0.159MetCys: 0.159 ± 0.011	1.259MetAsp: 1.259 ± 0.037	1.134MetGlu: 1.134 ± 0.029	0.755MetPhe: 0.755 ± 0.023	1.949MetGly: 1.949 ± 0.036	0.448MetHis: 0.448 ± 0.018	1.374MetIle: 1.374 ± 0.032	0.816MetLys: 0.816 ± 0.027	2.583MetLeu: 2.583 ± 0.046	0.659MetMet: 0.659 ± 0.025	0.7MetAsn: 0.7 ± 0.023	1.513MetPro: 1.513 ± 0.036	0.854MetGln: 0.854 ± 0.026	1.908MetArg: 1.908 ± 0.039	1.489MetSer: 1.489 ± 0.033	1.991MetThr: 1.991 ± 0.04	1.771MetVal: 1.771 ± 0.04	0.219MetTrp: 0.219 ± 0.012	0.351MetTyr: 0.351 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.818AsnAla: 2.818 ± 0.052	0.229AsnCys: 0.229 ± 0.015	1.167AsnAsp: 1.167 ± 0.041	0.939AsnGlu: 0.939 ± 0.028	0.788AsnPhe: 0.788 ± 0.022	1.943AsnGly: 1.943 ± 0.044	0.457AsnHis: 0.457 ± 0.019	1.082AsnIle: 1.082 ± 0.027	0.433AsnLys: 0.433 ± 0.018	2.277AsnLeu: 2.277 ± 0.042	0.588AsnMet: 0.588 ± 0.018	0.518AsnAsn: 0.518 ± 0.022	1.691AsnPro: 1.691 ± 0.038	0.547AsnGln: 0.547 ± 0.021	1.707AsnArg: 1.707 ± 0.036	0.924AsnSer: 0.924 ± 0.028	1.051AsnThr: 1.051 ± 0.032	1.62AsnVal: 1.62 ± 0.039	0.38AsnTrp: 0.38 ± 0.016	0.558AsnTyr: 0.558 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.743ProAla: 7.743 ± 0.113	0.4ProCys: 0.4 ± 0.018	4.286ProAsp: 4.286 ± 0.063	4.28ProGlu: 4.28 ± 0.067	1.953ProPhe: 1.953 ± 0.034	5.696ProGly: 5.696 ± 0.081	1.13ProHis: 1.13 ± 0.027	1.971ProIle: 1.971 ± 0.036	1.193ProLys: 1.193 ± 0.028	5.147ProLeu: 5.147 ± 0.067	1.251ProMet: 1.251 ± 0.028	1.139ProAsn: 1.139 ± 0.03	3.017ProPro: 3.017 ± 0.055	1.74ProGln: 1.74 ± 0.042	3.642ProArg: 3.642 ± 0.059	2.221ProSer: 2.221 ± 0.038	2.213ProThr: 2.213 ± 0.043	4.745ProVal: 4.745 ± 0.06	0.807ProTrp: 0.807 ± 0.025	1.095ProTyr: 1.095 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.234GlnAla: 4.234 ± 0.057	0.197GlnCys: 0.197 ± 0.011	1.666GlnAsp: 1.666 ± 0.039	1.361GlnGlu: 1.361 ± 0.037	0.875GlnPhe: 0.875 ± 0.028	2.447GlnGly: 2.447 ± 0.042	0.562GlnHis: 0.562 ± 0.019	1.742GlnIle: 1.742 ± 0.035	0.776GlnLys: 0.776 ± 0.026	2.711GlnLeu: 2.711 ± 0.043	0.918GlnMet: 0.918 ± 0.025	0.695GlnAsn: 0.695 ± 0.022	1.706GlnPro: 1.706 ± 0.037	1.033GlnGln: 1.033 ± 0.034	2.224GlnArg: 2.224 ± 0.043	1.289GlnSer: 1.289 ± 0.034	1.638GlnThr: 1.638 ± 0.034	2.454GlnVal: 2.454 ± 0.046	0.325GlnTrp: 0.325 ± 0.015	0.511GlnTyr: 0.511 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	10.695ArgAla: 10.695 ± 0.116	0.596ArgCys: 0.596 ± 0.023	4.804ArgAsp: 4.804 ± 0.065	3.989ArgGlu: 3.989 ± 0.061	2.99ArgPhe: 2.99 ± 0.051	5.856ArgGly: 5.856 ± 0.073	1.841ArgHis: 1.841 ± 0.043	4.253ArgIle: 4.253 ± 0.061	1.98ArgLys: 1.98 ± 0.041	8.832ArgLeu: 8.832 ± 0.1	2.185ArgMet: 2.185 ± 0.043	1.76ArgAsn: 1.76 ± 0.037	4.45ArgPro: 4.45 ± 0.062	2.59ArgGln: 2.59 ± 0.041	7.097ArgArg: 7.097 ± 0.102	3.37ArgSer: 3.37 ± 0.055	3.774ArgThr: 3.774 ± 0.052	5.302ArgVal: 5.302 ± 0.065	1.09ArgTrp: 1.09 ± 0.031	1.633ArgTyr: 1.633 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	5.762SerAla: 5.762 ± 0.073	0.438SerCys: 0.438 ± 0.018	2.75SerAsp: 2.75 ± 0.044	2.351SerGlu: 2.351 ± 0.037	1.89SerPhe: 1.89 ± 0.037	5.094SerGly: 5.094 ± 0.064	0.965SerHis: 0.965 ± 0.025	2.107SerIle: 2.107 ± 0.044	1.015SerLys: 1.015 ± 0.034	4.474SerLeu: 4.474 ± 0.067	1.164SerMet: 1.164 ± 0.029	1.089SerAsn: 1.089 ± 0.034	2.34SerPro: 2.34 ± 0.041	1.214SerGln: 1.214 ± 0.029	3.377SerArg: 3.377 ± 0.056	2.1SerSer: 2.1 ± 0.043	2.119SerThr: 2.119 ± 0.037	3.548SerVal: 3.548 ± 0.054	0.708SerTrp: 0.708 ± 0.021	1.109SerTyr: 1.109 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	6.578ThrAla: 6.578 ± 0.074	0.48ThrCys: 0.48 ± 0.02	2.982ThrAsp: 2.982 ± 0.055	2.807ThrGlu: 2.807 ± 0.045	1.734ThrPhe: 1.734 ± 0.043	5.615ThrGly: 5.615 ± 0.068	0.989ThrHis: 0.989 ± 0.028	2.213ThrIle: 2.213 ± 0.042	0.875ThrLys: 0.875 ± 0.029	5.787ThrLeu: 5.787 ± 0.077	1.126ThrMet: 1.126 ± 0.032	1.019ThrAsn: 1.019 ± 0.03	3.317ThrPro: 3.317 ± 0.045	1.227ThrGln: 1.227 ± 0.03	3.754ThrArg: 3.754 ± 0.055	2.228ThrSer: 2.228 ± 0.045	2.449ThrThr: 2.449 ± 0.049	4.183ThrVal: 4.183 ± 0.059	0.663ThrTrp: 0.663 ± 0.021	1.022ThrTyr: 1.022 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	9.981ValAla: 9.981 ± 0.1	0.663ValCys: 0.663 ± 0.025	4.076ValAsp: 4.076 ± 0.062	4.376ValGlu: 4.376 ± 0.059	2.832ValPhe: 2.832 ± 0.056	5.465ValGly: 5.465 ± 0.069	1.409ValHis: 1.409 ± 0.033	3.862ValIle: 3.862 ± 0.056	1.637ValLys: 1.637 ± 0.039	7.964ValLeu: 7.964 ± 0.097	1.864ValMet: 1.864 ± 0.039	1.695ValAsn: 1.695 ± 0.039	4.039ValPro: 4.039 ± 0.062	1.925ValGln: 1.925 ± 0.041	5.428ValArg: 5.428 ± 0.072	3.908ValSer: 3.908 ± 0.059	4.436ValThr: 4.436 ± 0.064	5.691ValVal: 5.691 ± 0.076	1.018ValTrp: 1.018 ± 0.027	1.515ValTyr: 1.515 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.501TrpAla: 1.501 ± 0.037	0.134TrpCys: 0.134 ± 0.009	0.793TrpAsp: 0.793 ± 0.024	0.576TrpGlu: 0.576 ± 0.02	0.514TrpPhe: 0.514 ± 0.024	1.063TrpGly: 1.063 ± 0.027	0.335TrpHis: 0.335 ± 0.015	0.696TrpIle: 0.696 ± 0.024	0.395TrpLys: 0.395 ± 0.018	1.726TrpLeu: 1.726 ± 0.041	0.362TrpMet: 0.362 ± 0.017	0.398TrpAsn: 0.398 ± 0.017	0.737TrpPro: 0.737 ± 0.022	0.591TrpGln: 0.591 ± 0.021	1.279TrpArg: 1.279 ± 0.035	0.772TrpSer: 0.772 ± 0.022	0.877TrpThr: 0.877 ± 0.028	0.923TrpVal: 0.923 ± 0.027	0.255TrpTrp: 0.255 ± 0.014	0.309TrpTyr: 0.309 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.571TyrAla: 2.571 ± 0.037	0.234TyrCys: 0.234 ± 0.013	1.48TyrAsp: 1.48 ± 0.038	1.083TyrGlu: 1.083 ± 0.034	0.79TyrPhe: 0.79 ± 0.022	2.001TyrGly: 2.001 ± 0.039	0.473TyrHis: 0.473 ± 0.017	0.771TyrIle: 0.771 ± 0.027	0.458TyrLys: 0.458 ± 0.02	2.201TyrLeu: 2.201 ± 0.038	0.419TyrMet: 0.419 ± 0.017	0.501TyrAsn: 0.501 ± 0.019	1.084TyrPro: 1.084 ± 0.029	0.632TyrGln: 0.632 ± 0.022	1.691TyrArg: 1.691 ± 0.033	0.944TyrSer: 0.944 ± 0.027	1.019TyrThr: 1.019 ± 0.026	1.455TyrVal: 1.455 ± 0.033	0.34TyrTrp: 0.34 ± 0.016	0.541TyrTyr: 0.541 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4349 proteins (1366446 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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