Thermus virus P23-77

Taxonomy: Viruses; Varidnaviria; Helvetiavirae; Dividoviricota; Laserviricetes; Halopanivirales; Sphaerolipoviridae; Gammasphaerolipovirus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8CHM7|C8CHM7_9VIRU VP29 OS=Thermus virus P23-77 OX=1714272 PE=4 SV=1
MM1 pKa = 7.96GDD3 pKa = 3.64TGDD6 pKa = 3.69GSYY9 pKa = 9.51LTLYY13 pKa = 10.22GYY15 pKa = 11.21AWLGLLDD22 pKa = 3.74RR23 pKa = 11.84KK24 pKa = 9.4EE25 pKa = 3.76NRR27 pKa = 11.84FRR29 pKa = 11.84LFQAQVPGEE38 pKa = 4.46GPWPLSDD45 pKa = 3.79PRR47 pKa = 11.84GPDD50 pKa = 2.92VAVWVEE56 pKa = 4.13QEE58 pKa = 4.45VPPLPHH64 pKa = 7.11DD65 pKa = 3.99PRR67 pKa = 11.84EE68 pKa = 4.07VRR70 pKa = 11.84HH71 pKa = 5.68MALAFDD77 pKa = 3.51QAARR81 pKa = 11.84HH82 pKa = 4.66VLAYY86 pKa = 10.1EE87 pKa = 3.97RR88 pKa = 11.84GGQVWVRR95 pKa = 11.84QWDD98 pKa = 3.65PVSQVFVMRR107 pKa = 11.84GPFPGVDD114 pKa = 3.1PFLVQDD120 pKa = 3.48ATVGYY125 pKa = 8.43FPPDD129 pKa = 3.19SDD131 pKa = 3.73VLLFHH136 pKa = 7.15LSPDD140 pKa = 3.03RR141 pKa = 11.84TALVMRR147 pKa = 11.84AQRR150 pKa = 11.84EE151 pKa = 4.49LYY153 pKa = 7.85ATPHH157 pKa = 5.82VIEE160 pKa = 4.71TFPSPVVLDD169 pKa = 3.56QAVALPYY176 pKa = 10.29QIEE179 pKa = 4.25LLGSFLDD186 pKa = 3.93ALGEE190 pKa = 4.14TGVVVRR196 pKa = 11.84SEE198 pKa = 4.38VYY200 pKa = 9.22PVRR203 pKa = 11.84VGDD206 pKa = 4.09ALGPATFTAPATGAYY221 pKa = 8.95IPVVVIQDD229 pKa = 4.45LGTDD233 pKa = 3.68TLGTGAFAAPTTGAYY248 pKa = 9.45IPVVVIQDD256 pKa = 4.35LGTEE260 pKa = 4.17ALGSGTFTAPSTGAYY275 pKa = 8.82IPVVVVQDD283 pKa = 4.38LGTEE287 pKa = 4.08ALATGTFTAPATGTYY302 pKa = 9.91VLVVVVQDD310 pKa = 3.92LTASQYY316 pKa = 10.67TDD318 pKa = 2.99GLGYY322 pKa = 10.11EE323 pKa = 4.32ALGTGTFAAPTTGSYY338 pKa = 9.27TLAA341 pKa = 5.26

Molecular weight: 36.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8CHL2|C8CHL2_9VIRU Uncharacterized protein OS=Thermus virus P23-77 OX=1714272 PE=4 SV=1
MM1 pKa = 7.85GGRR4 pKa = 11.84QTFRR8 pKa = 11.84LLIIGKK14 pKa = 9.17SGSGKK19 pKa = 8.61STLARR24 pKa = 11.84QVIRR28 pKa = 11.84AMEE31 pKa = 3.88GRR33 pKa = 11.84YY34 pKa = 8.96RR35 pKa = 11.84HH36 pKa = 5.78LVIVNRR42 pKa = 11.84KK43 pKa = 6.84TEE45 pKa = 3.93FAEE48 pKa = 3.97LAEE51 pKa = 4.04GRR53 pKa = 11.84FRR55 pKa = 11.84VKK57 pKa = 10.64EE58 pKa = 4.38DD59 pKa = 3.56GDD61 pKa = 3.85PGPALRR67 pKa = 11.84RR68 pKa = 11.84YY69 pKa = 9.59RR70 pKa = 11.84RR71 pKa = 11.84VHH73 pKa = 5.0FHH75 pKa = 4.4VTGYY79 pKa = 10.74DD80 pKa = 3.33PRR82 pKa = 11.84PFLDD86 pKa = 4.01ALGQEE91 pKa = 4.79LMRR94 pKa = 11.84MRR96 pKa = 11.84DD97 pKa = 3.26VLLVVDD103 pKa = 5.02EE104 pKa = 4.33AHH106 pKa = 6.4QFFPRR111 pKa = 11.84GQVPKK116 pKa = 10.89GLFEE120 pKa = 4.2VLTGGRR126 pKa = 11.84EE127 pKa = 3.82NGHH130 pKa = 5.49NVVFVTQMLKK140 pKa = 10.75GAVGGIDD147 pKa = 3.08PGVRR151 pKa = 11.84RR152 pKa = 11.84QASHH156 pKa = 6.64LVAFRR161 pKa = 11.84VTEE164 pKa = 4.02PAEE167 pKa = 4.15VASVAEE173 pKa = 4.34MFPEE177 pKa = 4.3LGEE180 pKa = 4.24RR181 pKa = 11.84VQHH184 pKa = 6.03LRR186 pKa = 11.84RR187 pKa = 11.84PEE189 pKa = 4.26GGLPPEE195 pKa = 4.36YY196 pKa = 10.36GVRR199 pKa = 11.84DD200 pKa = 3.75LDD202 pKa = 3.72RR203 pKa = 11.84DD204 pKa = 3.42RR205 pKa = 11.84SGLVLRR211 pKa = 11.84DD212 pKa = 3.19PRR214 pKa = 11.84DD215 pKa = 3.11PRR217 pKa = 11.84RR218 pKa = 11.84RR219 pKa = 11.84VWVPLL224 pKa = 3.52

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

5621

35

425

151.9

16.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.119 ± 0.663

0.747 ± 0.179

3.896 ± 0.256

5.675 ± 0.428

2.597 ± 0.267

9.998 ± 0.481

1.228 ± 0.155

3.434 ± 0.304

3.096 ± 0.474

10.532 ± 0.433

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.975 ± 0.146

1.939 ± 0.287

8.023 ± 0.511

4.341 ± 0.272

8.308 ± 0.692

4.892 ± 0.342

4.892 ± 0.461

8.308 ± 0.471

2.028 ± 0.207

2.971 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski