Scytonema tolypothrichoides VB-61278

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Scytonemataceae; Scytonema; Scytonema tolypothrichoides

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0C2M242|A0A0C2M242_9CYAN Ankyrin OS=Scytonema tolypothrichoides VB-61278 OX=1233231 GN=SD80_26705 PE=4 SV=1
MM1 pKa = 7.26ATYY4 pKa = 10.56KK5 pKa = 9.5VTLINDD11 pKa = 3.46AEE13 pKa = 4.92GINKK17 pKa = 8.82TIEE20 pKa = 4.13VPDD23 pKa = 4.14DD24 pKa = 3.89EE25 pKa = 5.48YY26 pKa = 11.12ILDD29 pKa = 3.65IAEE32 pKa = 4.68EE33 pKa = 4.2EE34 pKa = 4.59DD35 pKa = 3.87LDD37 pKa = 4.68LPYY40 pKa = 10.71SCRR43 pKa = 11.84SGSCSDD49 pKa = 3.57CAGLIKK55 pKa = 10.65SGTVDD60 pKa = 3.17QSDD63 pKa = 4.01GNFLDD68 pKa = 5.78DD69 pKa = 4.28DD70 pKa = 4.72QIDD73 pKa = 3.3QGYY76 pKa = 9.37VLTCIAKK83 pKa = 7.64PTSDD87 pKa = 3.45VTIEE91 pKa = 3.91THH93 pKa = 6.75KK94 pKa = 11.01KK95 pKa = 10.58DD96 pKa = 5.07DD97 pKa = 3.62IEE99 pKa = 4.06

Molecular weight:
10.85 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0C2M4I7|A0A0C2M4I7_9CYAN CopG family transcriptional regulator OS=Scytonema tolypothrichoides VB-61278 OX=1233231 GN=SD80_23470 PE=4 SV=1
MM1 pKa = 6.86QASLALRR8 pKa = 11.84NRR10 pKa = 11.84PSTKK14 pKa = 10.22LLCIFNAQQQGRR26 pKa = 11.84RR27 pKa = 11.84QTAEE31 pKa = 3.59GRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.99EE36 pKa = 4.16SFNDD40 pKa = 3.62GLNQNNMQVKK50 pKa = 9.93SATAYY55 pKa = 9.64DD56 pKa = 4.07QGWLAYY62 pKa = 9.49IRR64 pKa = 11.84TGG66 pKa = 3.39

Molecular weight:
7.5 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5625

2

5627

1720987

29

2331

305.8

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.105 ± 0.034

1.012 ± 0.011

4.728 ± 0.022

6.256 ± 0.03

3.998 ± 0.022

6.752 ± 0.036

1.911 ± 0.019

6.427 ± 0.025

4.928 ± 0.031

10.906 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.87 ± 0.014

4.18 ± 0.026

4.718 ± 0.025

5.358 ± 0.031

5.296 ± 0.028

6.4 ± 0.029

5.722 ± 0.025

7.045 ± 0.027

1.393 ± 0.016

2.994 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski