Bacillus phage Silence

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LMD7|A0A0K1LMD7_9CAUD Endolysin OS=Bacillus phage Silence OX=1675599 GN=CPT_Silence26 PE=4 SV=1
MM1 pKa = 7.14EE2 pKa = 6.23HH3 pKa = 6.93EE4 pKa = 4.39LQDD7 pKa = 3.37IFKK10 pKa = 9.84MYY12 pKa = 10.51IEE14 pKa = 4.48VDD16 pKa = 3.47EE17 pKa = 4.57QGNITDD23 pKa = 4.07SLGGANVVPLKK34 pKa = 10.77DD35 pKa = 3.46YY36 pKa = 10.84TFFFLDD42 pKa = 3.48TAVVEE47 pKa = 4.25GEE49 pKa = 4.18EE50 pKa = 4.3MILPLVMEE58 pKa = 4.65NVFKK62 pKa = 11.13LKK64 pKa = 10.75LVINGFKK71 pKa = 10.65PEE73 pKa = 3.93FVLKK77 pKa = 10.7DD78 pKa = 3.7SNDD81 pKa = 3.49EE82 pKa = 4.18LTLEE86 pKa = 4.2MPGIAVFNDD95 pKa = 3.36DD96 pKa = 3.89PTTGEE101 pKa = 3.99EE102 pKa = 4.44TVV104 pKa = 3.3

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LLK3|A0A0K1LLK3_9CAUD DNA helicase OS=Bacillus phage Silence OX=1675599 GN=CPT_Silence54 PE=3 SV=1
MM1 pKa = 7.45IRR3 pKa = 11.84ALKK6 pKa = 10.23LAFIAFLSLILWLSIIGWIKK26 pKa = 10.07LIWRR30 pKa = 11.84ALHH33 pKa = 6.42EE34 pKa = 4.53FFF36 pKa = 6.11

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12515

30

1004

189.6

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.343 ± 0.659

0.871 ± 0.145

5.857 ± 0.324

6.92 ± 0.496

4.187 ± 0.186

7.191 ± 0.279

1.582 ± 0.124

7.127 ± 0.354

8.638 ± 0.528

8.326 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.14 ± 0.322

5.753 ± 0.257

3.044 ± 0.184

3.668 ± 0.352

4.251 ± 0.234

5.425 ± 0.258

5.865 ± 0.283

6.177 ± 0.224

1.159 ± 0.096

3.476 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski