Sphingobium amiense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3954 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494W7Y4|A0A494W7Y4_9SPHN DUF3617 domain-containing protein OS=Sphingobium amiense OX=135719 GN=SAMIE_1027590 PE=4 SV=1
MM1 pKa = 7.79PKK3 pKa = 10.4LIVVNRR9 pKa = 11.84AGEE12 pKa = 3.97EE13 pKa = 3.86QEE15 pKa = 4.08IDD17 pKa = 3.64GEE19 pKa = 4.58TGLSVMEE26 pKa = 4.73VIRR29 pKa = 11.84DD30 pKa = 3.56HH31 pKa = 7.32GFDD34 pKa = 4.23EE35 pKa = 5.43LLALCGGCCSCATCHH50 pKa = 6.48VYY52 pKa = 10.52VDD54 pKa = 3.85PAFAGALPPMSEE66 pKa = 5.07DD67 pKa = 3.85EE68 pKa = 4.43NDD70 pKa = 4.07LLDD73 pKa = 4.81SSDD76 pKa = 3.59HH77 pKa = 6.39RR78 pKa = 11.84NEE80 pKa = 3.85TSRR83 pKa = 11.84LSCQVQLTGALDD95 pKa = 3.67GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494WAD8|A0A494WAD8_9SPHN WYL domain-containing protein OS=Sphingobium amiense OX=135719 GN=SAMIE_1031180 PE=4 SV=1
MM1 pKa = 7.07TALATHH7 pKa = 7.09LAAFLRR13 pKa = 11.84EE14 pKa = 3.96HH15 pKa = 7.16LPRR18 pKa = 11.84EE19 pKa = 4.51RR20 pKa = 11.84NVSPHH25 pKa = 5.41TVTTYY30 pKa = 11.44ANCFALLVRR39 pKa = 11.84FAANHH44 pKa = 6.14LKK46 pKa = 10.3RR47 pKa = 11.84RR48 pKa = 11.84PTDD51 pKa = 3.35LTIEE55 pKa = 4.55DD56 pKa = 4.72FDD58 pKa = 4.7PALIMAFLDD67 pKa = 3.46HH68 pKa = 7.92SEE70 pKa = 4.19EE71 pKa = 3.93ARR73 pKa = 11.84KK74 pKa = 10.01NSPRR78 pKa = 11.84TRR80 pKa = 11.84NARR83 pKa = 11.84LAAIRR88 pKa = 11.84AFFRR92 pKa = 11.84YY93 pKa = 9.38VEE95 pKa = 4.05YY96 pKa = 10.4RR97 pKa = 11.84VPACLDD103 pKa = 3.15LALRR107 pKa = 11.84VRR109 pKa = 11.84AVPTKK114 pKa = 10.23RR115 pKa = 11.84TDD117 pKa = 3.1TTLIDD122 pKa = 3.61YY123 pKa = 7.56LTRR126 pKa = 11.84DD127 pKa = 4.29EE128 pKa = 4.47ITALLDD134 pKa = 3.88APDD137 pKa = 4.11PQSRR141 pKa = 11.84LGTRR145 pKa = 11.84DD146 pKa = 2.87RR147 pKa = 11.84AMLHH151 pKa = 6.15LAYY154 pKa = 10.37AGGLRR159 pKa = 11.84VAEE162 pKa = 4.2LLSLQMQDD170 pKa = 3.17FPEE173 pKa = 4.79RR174 pKa = 11.84SLATVHH180 pKa = 6.97IIGKK184 pKa = 8.23GRR186 pKa = 11.84RR187 pKa = 11.84EE188 pKa = 4.05RR189 pKa = 11.84ILPLWRR195 pKa = 11.84EE196 pKa = 4.12TQASLRR202 pKa = 11.84AWLAVRR208 pKa = 11.84PQCQAMEE215 pKa = 4.28IFLNAHH221 pKa = 6.44GEE223 pKa = 3.96PMTRR227 pKa = 11.84DD228 pKa = 3.04GFAFRR233 pKa = 11.84LAKK236 pKa = 10.24HH237 pKa = 5.52VATAAKK243 pKa = 9.25KK244 pKa = 10.13QPSLLRR250 pKa = 11.84KK251 pKa = 9.25RR252 pKa = 11.84VTPHH256 pKa = 5.67VLRR259 pKa = 11.84HH260 pKa = 4.92SCAMHH265 pKa = 6.13TLAATGDD272 pKa = 3.51IRR274 pKa = 11.84KK275 pKa = 8.74VALWLGHH282 pKa = 6.83ASIQSTEE289 pKa = 3.76AYY291 pKa = 10.05LRR293 pKa = 11.84ADD295 pKa = 3.93PEE297 pKa = 4.18EE298 pKa = 4.21KK299 pKa = 10.66LQILAAHH306 pKa = 7.17GSPLVKK312 pKa = 9.78PGRR315 pKa = 11.84FRR317 pKa = 11.84PPADD321 pKa = 3.92PLIMMLNDD329 pKa = 2.95VRR331 pKa = 11.84KK332 pKa = 9.13RR333 pKa = 11.84ASPRR337 pKa = 11.84GRR339 pKa = 11.84SAPGIQSPP347 pKa = 3.87

Molecular weight: 39.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3954

0

3954

1349417

100

2803

341.3

36.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.277 ± 0.063

0.795 ± 0.012

6.055 ± 0.029

5.302 ± 0.035

3.512 ± 0.026

8.79 ± 0.039

2.078 ± 0.02

5.013 ± 0.025

2.896 ± 0.028

9.979 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.019

2.455 ± 0.024

5.372 ± 0.03

3.224 ± 0.021

7.622 ± 0.043

5.304 ± 0.027

5.078 ± 0.028

7.024 ± 0.031

1.45 ± 0.015

2.262 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski