Oryza barthii

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3HSL5|A0A0D3HSL5_9ORYZ LRRNT_2 domain-containing protein OS=Oryza barthii OX=65489 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 6.25ADD4 pKa = 4.51DD5 pKa = 5.13DD6 pKa = 4.69VSTAALTDD14 pKa = 3.75GGHH17 pKa = 5.17GQRR20 pKa = 11.84HH21 pKa = 5.88RR22 pKa = 11.84FTFLLPSQNAMMPPAPPTSIDD43 pKa = 4.76DD44 pKa = 3.95EE45 pKa = 7.24DD46 pKa = 6.48DD47 pKa = 4.38DD48 pKa = 5.57FSPDD52 pKa = 3.4DD53 pKa = 3.48VAQILSHH60 pKa = 6.81LLLNGVISGEE70 pKa = 4.24TALLLQILTVALHH83 pKa = 6.51FDD85 pKa = 3.55LGGGGGGGGHH95 pKa = 5.93GQNNDD100 pKa = 3.38EE101 pKa = 4.84DD102 pKa = 5.59AMMAAPPPSIDD113 pKa = 5.95DD114 pKa = 4.01EE115 pKa = 7.14DD116 pKa = 6.7DD117 pKa = 4.98DD118 pKa = 6.21DD119 pKa = 6.1GSPLLDD125 pKa = 3.57QVLCYY130 pKa = 10.68LLLNGIISGEE140 pKa = 3.89RR141 pKa = 11.84ALQILQNANMPLDD154 pKa = 4.56LDD156 pKa = 5.07LDD158 pKa = 4.33LEE160 pKa = 4.91DD161 pKa = 5.26GGANMPLDD169 pKa = 4.68LDD171 pKa = 4.24DD172 pKa = 5.24GGGFRR177 pKa = 11.84GVPASAAAVAGLEE190 pKa = 4.06KK191 pKa = 10.55QVFHH195 pKa = 6.84QFDD198 pKa = 3.46HH199 pKa = 7.13HH200 pKa = 7.77GGDD203 pKa = 5.27DD204 pKa = 5.04DD205 pKa = 7.14DD206 pKa = 6.84DD207 pKa = 6.95DD208 pKa = 4.16EE209 pKa = 6.75AKK211 pKa = 10.77DD212 pKa = 3.65SAAGCVICMEE222 pKa = 4.29EE223 pKa = 4.44FVAGDD228 pKa = 3.92EE229 pKa = 4.52VCAIPCAGNHH239 pKa = 5.69SFHH242 pKa = 6.58HH243 pKa = 5.99HH244 pKa = 7.39CITEE248 pKa = 3.92WLGCSNVCPLCRR260 pKa = 11.84HH261 pKa = 6.44ALPVEE266 pKa = 4.21EE267 pKa = 4.25QDD269 pKa = 4.12EE270 pKa = 4.77GGATT274 pKa = 3.39

Molecular weight: 28.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3HWM7|A0A0D3HWM7_9ORYZ Uncharacterized protein OS=Oryza barthii OX=65489 PE=4 SV=1
MM1 pKa = 7.67AWRR4 pKa = 11.84GAASRR9 pKa = 11.84TVLAAVRR16 pKa = 11.84RR17 pKa = 11.84PGPSPSAAARR27 pKa = 11.84ALRR30 pKa = 11.84APPPVAAPRR39 pKa = 11.84PAASHH44 pKa = 6.7PPSPPPPRR52 pKa = 11.84RR53 pKa = 11.84PHH55 pKa = 5.43GRR57 pKa = 11.84WRR59 pKa = 11.84RR60 pKa = 3.42

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34345

5869

40214

16234674

7

5436

403.7

44.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.824 ± 0.019

1.949 ± 0.006

5.422 ± 0.009

6.034 ± 0.012

3.683 ± 0.008

7.287 ± 0.014

2.5 ± 0.006

4.615 ± 0.01

4.956 ± 0.01

9.634 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.004

3.66 ± 0.011

5.368 ± 0.011

3.449 ± 0.01

6.245 ± 0.014

8.36 ± 0.015

4.849 ± 0.008

6.74 ± 0.01

1.325 ± 0.005

2.6 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski