Lutibacter sp. HS1-25

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Lutibacter; unclassified Lutibacter

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4V1MWV7|A0A4V1MWV7_9FLAO Peptidase M23 OS=Lutibacter sp. HS1-25 OX=2485000 GN=EC396_00325 PE=4 SV=1
MM1 pKa = 7.44IKK3 pKa = 10.21SIKK6 pKa = 10.05LAILSCFFILPVCAQTTIDD25 pKa = 3.75SFDD28 pKa = 3.77FEE30 pKa = 4.95FGLDD34 pKa = 3.14SWTQSAGDD42 pKa = 4.29DD43 pKa = 3.62FDD45 pKa = 3.95LTRR48 pKa = 11.84LSGGTPSAGTGPNIASSGNYY68 pKa = 9.92YY69 pKa = 9.8MFAEE73 pKa = 4.44MSSPRR78 pKa = 11.84LNGDD82 pKa = 3.22VAIFTSPVIDD92 pKa = 3.66LAGYY96 pKa = 6.92MQPTFSFDD104 pKa = 3.19YY105 pKa = 10.91HH106 pKa = 6.27MYY108 pKa = 9.96SSNSISDD115 pKa = 3.55VGTLALEE122 pKa = 4.13INEE125 pKa = 4.93NNTGWQSTSFSLTGSQNVDD144 pKa = 2.94QNTWLSGLVDD154 pKa = 4.2LLAYY158 pKa = 10.18QGSLVQFRR166 pKa = 11.84FVITRR171 pKa = 11.84ANSWQGDD178 pKa = 3.57IAFDD182 pKa = 4.05NIAIEE187 pKa = 4.37GTALVAGVEE196 pKa = 4.64IYY198 pKa = 11.03LSGNGISIPSGKK210 pKa = 7.99TAFSVTDD217 pKa = 3.58GTDD220 pKa = 3.5FGSVNLASSSIAQTFTIEE238 pKa = 4.16NWGLDD243 pKa = 3.38NLVLNNSPYY252 pKa = 10.91VVITGADD259 pKa = 3.21AANFSVDD266 pKa = 3.69LSSTTSPISSANSTTFQVTFSPLSLGSKK294 pKa = 8.18TATIQISNNDD304 pKa = 3.66SDD306 pKa = 3.78EE307 pKa = 4.2SLYY310 pKa = 10.63TFNIAGFGVQNFFDD324 pKa = 4.08SDD326 pKa = 3.42GDD328 pKa = 4.37GIFDD332 pKa = 4.95NVDD335 pKa = 2.89IDD337 pKa = 4.85DD338 pKa = 5.23DD339 pKa = 4.41NDD341 pKa = 4.77GILDD345 pKa = 4.54AIEE348 pKa = 4.86EE349 pKa = 4.4NTCKK353 pKa = 10.39NSSISIPANYY363 pKa = 9.81KK364 pKa = 10.02FLNEE368 pKa = 4.19TFGTLTPGTRR378 pKa = 11.84TTINTSYY385 pKa = 11.18NATTTYY391 pKa = 10.17CYY393 pKa = 10.69EE394 pKa = 4.71PGTGSGSSCGSTWILDD410 pKa = 3.56DD411 pKa = 3.85GEE413 pKa = 4.6YY414 pKa = 11.04VVVSKK419 pKa = 10.81IAGTIASDD427 pKa = 3.57PEE429 pKa = 4.56NIHH432 pKa = 7.35GDD434 pKa = 3.94LAWTNVLDD442 pKa = 3.77HH443 pKa = 6.87TPNDD447 pKa = 3.82GDD449 pKa = 3.75TGGMAVFNAAWAPGVFYY466 pKa = 8.22EE467 pKa = 4.4TTITGILPNVPITYY481 pKa = 10.16SFWVLNIMAQSTYY494 pKa = 10.78SGSILPNVTVQFLDD508 pKa = 3.48LLGNEE513 pKa = 4.18ITSIDD518 pKa = 3.23TGEE521 pKa = 4.41FGRR524 pKa = 11.84CAAGTGDD531 pKa = 3.55NTCAEE536 pKa = 4.35NEE538 pKa = 4.16WQNFTRR544 pKa = 11.84NDD546 pKa = 3.25IVLGNVDD553 pKa = 3.38KK554 pKa = 11.12FIVRR558 pKa = 11.84FINNAPGGAGNDD570 pKa = 3.6LALDD574 pKa = 5.07DD575 pKa = 4.62IVISQTLCDD584 pKa = 3.73TDD586 pKa = 3.75SDD588 pKa = 4.29GVADD592 pKa = 5.64IFDD595 pKa = 5.05LDD597 pKa = 4.12SDD599 pKa = 3.99NDD601 pKa = 4.68GIPDD605 pKa = 3.57VVEE608 pKa = 4.64AGLGSLSAGTAKK620 pKa = 10.29IPFPSGWVDD629 pKa = 3.55ANANGMHH636 pKa = 7.21DD637 pKa = 3.66AAEE640 pKa = 4.41GNIPLDD646 pKa = 3.52SDD648 pKa = 4.21GDD650 pKa = 3.94GTPNYY655 pKa = 10.45LDD657 pKa = 5.03LDD659 pKa = 4.06SDD661 pKa = 3.74NDD663 pKa = 4.41SIFDD667 pKa = 3.61VDD669 pKa = 3.79EE670 pKa = 4.8SGAGNIANATFQNGDD685 pKa = 3.08GDD687 pKa = 3.46ISGNGVGQGPDD698 pKa = 2.89TDD700 pKa = 4.1YY701 pKa = 11.49VRR703 pKa = 11.84EE704 pKa = 3.97TDD706 pKa = 3.81YY707 pKa = 11.64EE708 pKa = 4.38SDD710 pKa = 3.24GTLEE714 pKa = 4.05YY715 pKa = 9.41FTDD718 pKa = 5.98GILDD722 pKa = 3.41IYY724 pKa = 11.16DD725 pKa = 3.77YY726 pKa = 11.56FNGNTFEE733 pKa = 4.09TAYY736 pKa = 10.72GNEE739 pKa = 3.98NQGLGNTYY747 pKa = 10.65YY748 pKa = 11.28VLDD751 pKa = 4.37SDD753 pKa = 5.12ADD755 pKa = 4.41GIPDD759 pKa = 3.75YY760 pKa = 11.02MDD762 pKa = 3.21VTSDD766 pKa = 3.26GVTFDD771 pKa = 4.11ISHH774 pKa = 6.79TLYY777 pKa = 11.37ANLDD781 pKa = 3.77ANNDD785 pKa = 3.56GVIDD789 pKa = 3.99DD790 pKa = 4.71TNDD793 pKa = 3.3SEE795 pKa = 5.17GDD797 pKa = 3.93GIVDD801 pKa = 5.49LFDD804 pKa = 3.75TSDD807 pKa = 3.23ATFGSPRR814 pKa = 11.84DD815 pKa = 3.7LDD817 pKa = 4.11RR818 pKa = 11.84KK819 pKa = 9.12LHH821 pKa = 6.43LYY823 pKa = 10.35FDD825 pKa = 3.98GRR827 pKa = 11.84NDD829 pKa = 3.51YY830 pKa = 11.49AEE832 pKa = 5.57DD833 pKa = 3.46IAVINAWGEE842 pKa = 3.82VSMMCWVKK850 pKa = 10.35IDD852 pKa = 3.96PSSTGIQHH860 pKa = 7.41ILGQNEE866 pKa = 3.83FFIQLNADD874 pKa = 3.56KK875 pKa = 9.96TISAHH880 pKa = 6.97ASGSNLNYY888 pKa = 8.69STPLTLNQWVHH899 pKa = 5.92IATTYY904 pKa = 10.78SNSNNEE910 pKa = 3.74FKK912 pKa = 11.14LFINGQEE919 pKa = 3.99IAVDD923 pKa = 3.53TGISGSLSADD933 pKa = 2.98TSLFTLGKK941 pKa = 10.15IPGGNSNFYY950 pKa = 10.26HH951 pKa = 7.08GYY953 pKa = 8.77LDD955 pKa = 3.6EE956 pKa = 4.2VRR958 pKa = 11.84VFNKK962 pKa = 10.34ALSTNEE968 pKa = 3.76LQKK971 pKa = 10.41IVYY974 pKa = 9.33QEE976 pKa = 3.94IEE978 pKa = 4.2NNSGIVRR985 pKa = 11.84GSIIPMDD992 pKa = 3.43VTNYY996 pKa = 9.77IDD998 pKa = 3.63EE999 pKa = 4.53ATVNPLDD1006 pKa = 3.29WTYY1009 pKa = 10.73LQRR1012 pKa = 11.84YY1013 pKa = 8.32FKK1015 pKa = 10.27MDD1017 pKa = 3.17VYY1019 pKa = 11.09KK1020 pKa = 10.94DD1021 pKa = 4.54DD1022 pKa = 5.66IIDD1025 pKa = 4.4DD1026 pKa = 4.51LSTATVDD1033 pKa = 2.75IGTGAKK1039 pKa = 9.22IYY1041 pKa = 10.51NMKK1044 pKa = 10.44IIDD1047 pKa = 3.94VQNAPLPFVTNQSGTLPTAVNHH1069 pKa = 6.82SINGVNGNDD1078 pKa = 3.34VITYY1082 pKa = 8.81DD1083 pKa = 3.27WSIVKK1088 pKa = 9.81ILHH1091 pKa = 7.1DD1092 pKa = 4.36DD1093 pKa = 3.87VYY1095 pKa = 11.48FNNNQKK1101 pKa = 10.33HH1102 pKa = 5.96LGLFVNQLDD1111 pKa = 3.83ASLNPIEE1118 pKa = 6.2FSIQNNSEE1126 pKa = 4.65LNVSWYY1132 pKa = 10.84LKK1134 pKa = 10.65LDD1136 pKa = 3.44GFIDD1140 pKa = 4.87LEE1142 pKa = 4.64GKK1144 pKa = 9.29SQLVQGAEE1152 pKa = 4.12SVLDD1156 pKa = 3.94EE1157 pKa = 5.24DD1158 pKa = 3.7SAGYY1162 pKa = 9.83IEE1164 pKa = 5.33QDD1166 pKa = 3.32QQGTQSSYY1174 pKa = 11.38NYY1176 pKa = 10.26NYY1178 pKa = 7.06WTSSVSPIAAGAGTNNASNAIAEE1201 pKa = 4.29VLLDD1205 pKa = 3.6GTIEE1209 pKa = 4.28SNPVSINFKK1218 pKa = 9.53PAYY1221 pKa = 7.47TAADD1225 pKa = 3.86GAVTSPITLSSYY1237 pKa = 9.23WLYY1240 pKa = 10.6TFNGTNSDD1248 pKa = 3.39YY1249 pKa = 11.46DD1250 pKa = 3.41SWVSINEE1257 pKa = 3.74NSLLKK1262 pKa = 10.77AGEE1265 pKa = 4.72GYY1267 pKa = 8.83TMKK1270 pKa = 9.84GTSGVASITDD1280 pKa = 3.42CQNYY1284 pKa = 8.29VFKK1287 pKa = 10.91GKK1289 pKa = 8.78PHH1291 pKa = 6.57NGDD1294 pKa = 2.56ITLNIAINNDD1304 pKa = 3.35RR1305 pKa = 11.84LVGNPYY1311 pKa = 10.32PSALDD1316 pKa = 3.44ANAFILDD1323 pKa = 4.37NINDD1327 pKa = 4.18CGGNAATNIFNGALYY1342 pKa = 10.04FWDD1345 pKa = 3.69HH1346 pKa = 6.55FSGKK1350 pKa = 6.89THH1352 pKa = 6.62ILKK1355 pKa = 10.36EE1356 pKa = 4.22YY1357 pKa = 8.51VGGYY1361 pKa = 7.51ATYY1364 pKa = 9.86TIMGGAKK1371 pKa = 9.81AYY1373 pKa = 10.75ANDD1376 pKa = 3.37EE1377 pKa = 4.47RR1378 pKa = 11.84INNNGSSGVKK1388 pKa = 9.86VPEE1391 pKa = 4.03RR1392 pKa = 11.84YY1393 pKa = 9.38IPANQGFFVVAAIDD1407 pKa = 3.83TVSNSINTSVTGGTITFKK1425 pKa = 10.83NSQRR1429 pKa = 11.84KK1430 pKa = 9.28CIIEE1434 pKa = 4.12DD1435 pKa = 3.35SGNSVFLKK1443 pKa = 9.51STHH1446 pKa = 5.98KK1447 pKa = 10.81KK1448 pKa = 9.34EE1449 pKa = 4.51AIKK1452 pKa = 10.27EE1453 pKa = 4.08AIIEE1457 pKa = 3.99EE1458 pKa = 4.11DD1459 pKa = 3.44TRR1461 pKa = 11.84PKK1463 pKa = 10.23IRR1465 pKa = 11.84LLFKK1469 pKa = 10.93SPTGYY1474 pKa = 10.22EE1475 pKa = 3.86RR1476 pKa = 11.84QLLVGADD1483 pKa = 3.49QNASNDD1489 pKa = 3.06FDD1491 pKa = 4.48LGYY1494 pKa = 10.85DD1495 pKa = 3.51GLLIEE1500 pKa = 6.21DD1501 pKa = 4.02YY1502 pKa = 9.79TADD1505 pKa = 3.68MFWTFSGAKK1514 pKa = 9.45FVIQAIKK1521 pKa = 10.67EE1522 pKa = 3.98FDD1524 pKa = 3.41TNQQLKK1530 pKa = 10.59LGLVVEE1536 pKa = 4.71ANGLTTISIDD1546 pKa = 3.39SLEE1549 pKa = 4.37NFDD1552 pKa = 5.11TNTKK1556 pKa = 10.33CYY1558 pKa = 10.21IYY1560 pKa = 10.96DD1561 pKa = 3.48NLTNKK1566 pKa = 9.97TYY1568 pKa = 11.3DD1569 pKa = 3.44LTGNTAQIYY1578 pKa = 10.22LEE1580 pKa = 4.44AGTYY1584 pKa = 10.26LDD1586 pKa = 5.15RR1587 pKa = 11.84FDD1589 pKa = 6.38LRR1591 pKa = 11.84FKK1593 pKa = 10.67AQNTLAIDD1601 pKa = 3.64NAEE1604 pKa = 3.78ILANKK1609 pKa = 9.13IQVFVNNNSNEE1620 pKa = 3.94LQINNNLNTDD1630 pKa = 3.13IQSINLYY1637 pKa = 10.39NSLGQKK1643 pKa = 7.26MHH1645 pKa = 5.7TWNKK1649 pKa = 9.78NLQNTQLVFNLNIKK1663 pKa = 6.34TTGVYY1668 pKa = 8.25MAQVNTTEE1676 pKa = 4.18GSFTKK1681 pKa = 10.41KK1682 pKa = 10.71VIIEE1686 pKa = 4.0

Molecular weight:
182.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q1IQB9|A0A4Q1IQB9_9FLAO SusC/RagA family TonB-linked outer membrane protein OS=Lutibacter sp. HS1-25 OX=2485000 GN=EC396_02435 PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 3.92TITTDD27 pKa = 3.73KK28 pKa = 10.77PEE30 pKa = 4.26KK31 pKa = 10.61SLLTSNKK38 pKa = 9.63RR39 pKa = 11.84SGGRR43 pKa = 11.84NNKK46 pKa = 9.29GKK48 pKa = 8.29MTMRR52 pKa = 11.84YY53 pKa = 9.26IGGGHH58 pKa = 6.44KK59 pKa = 9.77KK60 pKa = 9.28QYY62 pKa = 10.44RR63 pKa = 11.84IIDD66 pKa = 3.77FKK68 pKa = 11.12RR69 pKa = 11.84NKK71 pKa = 10.0NGIPATVKK79 pKa = 10.05TIEE82 pKa = 4.0YY83 pKa = 9.86DD84 pKa = 3.47PNRR87 pKa = 11.84TAFIALLNYY96 pKa = 10.38ADD98 pKa = 3.68GEE100 pKa = 4.11KK101 pKa = 10.25RR102 pKa = 11.84YY103 pKa = 10.21IIAQNGLQVGQTVMSGEE120 pKa = 4.22GATPDD125 pKa = 3.47VGNTMLLSEE134 pKa = 4.99IPLGTTISCIEE145 pKa = 3.88LHH147 pKa = 6.66PGQGAVMARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.52YY171 pKa = 9.12ATVKK175 pKa = 10.42LPSGEE180 pKa = 3.83TRR182 pKa = 11.84MILLTCSATIGVVSNSDD199 pKa = 3.21HH200 pKa = 6.09QLLVSGKK207 pKa = 9.49AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVVMNPVDD229 pKa = 3.47HH230 pKa = 7.05PMGGGEE236 pKa = 4.28GKK238 pKa = 10.51SSGGHH243 pKa = 4.59PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.41GFKK256 pKa = 9.24TRR258 pKa = 11.84SKK260 pKa = 9.37TKK262 pKa = 10.4ASNKK266 pKa = 9.95YY267 pKa = 9.56IIEE270 pKa = 4.06RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.1KK274 pKa = 9.82

Molecular weight:
29.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3423

0

3423

1211269

23

2492

353.9

39.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.227 ± 0.036

0.774 ± 0.012

5.292 ± 0.031

6.473 ± 0.042

5.29 ± 0.031

6.338 ± 0.04

1.629 ± 0.019

8.462 ± 0.046

8.097 ± 0.052

9.086 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.021

6.932 ± 0.047

3.319 ± 0.026

3.252 ± 0.024

2.915 ± 0.024

6.433 ± 0.034

5.89 ± 0.043

6.229 ± 0.033

1.093 ± 0.016

4.182 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski