Amino acid dipepetide frequency for butyrate-producing bacterium SS3/4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.456AlaAla: 9.456 ± 0.161	1.149AlaCys: 1.149 ± 0.041	5.236AlaAsp: 5.236 ± 0.091	6.192AlaGlu: 6.192 ± 0.092	3.247AlaPhe: 3.247 ± 0.066	7.008AlaGly: 7.008 ± 0.105	1.205AlaHis: 1.205 ± 0.04	4.814AlaIle: 4.814 ± 0.088	5.494AlaLys: 5.494 ± 0.078	7.127AlaLeu: 7.127 ± 0.111	2.725AlaMet: 2.725 ± 0.055	2.553AlaAsn: 2.553 ± 0.054	2.378AlaPro: 2.378 ± 0.064	2.208AlaGln: 2.208 ± 0.052	3.066AlaArg: 3.066 ± 0.054	4.163AlaSer: 4.163 ± 0.078	3.251AlaThr: 3.251 ± 0.073	7.049AlaVal: 7.049 ± 0.107	0.693AlaTrp: 0.693 ± 0.03	2.701AlaTyr: 2.701 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.092CysAla: 1.092 ± 0.036	0.273CysCys: 0.273 ± 0.021	0.814CysAsp: 0.814 ± 0.035	0.905CysGlu: 0.905 ± 0.032	0.613CysPhe: 0.613 ± 0.025	1.574CysGly: 1.574 ± 0.046	0.326CysHis: 0.326 ± 0.023	0.901CysIle: 0.901 ± 0.032	0.757CysLys: 0.757 ± 0.031	1.203CysLeu: 1.203 ± 0.042	0.479CysMet: 0.479 ± 0.025	0.486CysAsn: 0.486 ± 0.025	0.672CysPro: 0.672 ± 0.033	0.39CysGln: 0.39 ± 0.023	0.828CysArg: 0.828 ± 0.036	0.868CysSer: 0.868 ± 0.037	0.773CysThr: 0.773 ± 0.03	1.065CysVal: 1.065 ± 0.037	0.097CysTrp: 0.097 ± 0.01	0.511CysTyr: 0.511 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.485AspAla: 4.485 ± 0.079	0.804AspCys: 0.804 ± 0.034	2.942AspAsp: 2.942 ± 0.073	4.66AspGlu: 4.66 ± 0.094	2.492AspPhe: 2.492 ± 0.05	5.052AspGly: 5.052 ± 0.097	1.094AspHis: 1.094 ± 0.043	3.857AspIle: 3.857 ± 0.073	3.285AspLys: 3.285 ± 0.069	4.88AspLeu: 4.88 ± 0.096	1.992AspMet: 1.992 ± 0.044	1.903AspAsn: 1.903 ± 0.05	2.15AspPro: 2.15 ± 0.051	1.412AspGln: 1.412 ± 0.037	2.627AspArg: 2.627 ± 0.054	3.048AspSer: 3.048 ± 0.074	2.992AspThr: 2.992 ± 0.056	3.867AspVal: 3.867 ± 0.084	0.537AspTrp: 0.537 ± 0.026	2.54AspTyr: 2.54 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	5.733GluAla: 5.733 ± 0.092	0.871GluCys: 0.871 ± 0.034	4.028GluAsp: 4.028 ± 0.076	6.732GluGlu: 6.732 ± 0.123	2.663GluPhe: 2.663 ± 0.059	4.159GluGly: 4.159 ± 0.084	1.425GluHis: 1.425 ± 0.044	5.52GluIle: 5.52 ± 0.099	6.976GluLys: 6.976 ± 0.118	6.652GluLeu: 6.652 ± 0.109	2.474GluMet: 2.474 ± 0.068	4.187GluAsn: 4.187 ± 0.074	1.935GluPro: 1.935 ± 0.057	2.669GluGln: 2.669 ± 0.063	3.671GluArg: 3.671 ± 0.07	3.506GluSer: 3.506 ± 0.061	4.355GluThr: 4.355 ± 0.087	4.366GluVal: 4.366 ± 0.086	0.699GluTrp: 0.699 ± 0.027	2.889GluTyr: 2.889 ± 0.066	0.0GluXaa: 0.0 ± 0.0
Phe	3.186PheAla: 3.186 ± 0.069	0.817PheCys: 0.817 ± 0.033	2.402PheAsp: 2.402 ± 0.051	2.622PheGlu: 2.622 ± 0.066	1.727PhePhe: 1.727 ± 0.055	3.279PheGly: 3.279 ± 0.06	0.862PheHis: 0.862 ± 0.037	2.466PheIle: 2.466 ± 0.06	1.956PheLys: 1.956 ± 0.046	4.145PheLeu: 4.145 ± 0.083	1.26PheMet: 1.26 ± 0.045	1.405PheAsn: 1.405 ± 0.042	1.426PhePro: 1.426 ± 0.043	1.18PheGln: 1.18 ± 0.032	1.889PheArg: 1.889 ± 0.059	2.738PheSer: 2.738 ± 0.054	2.329PheThr: 2.329 ± 0.057	2.829PheVal: 2.829 ± 0.065	0.393PheTrp: 0.393 ± 0.021	1.683PheTyr: 1.683 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	5.645GlyAla: 5.645 ± 0.092	1.242GlyCys: 1.242 ± 0.047	3.762GlyAsp: 3.762 ± 0.073	4.937GlyGlu: 4.937 ± 0.086	3.171GlyPhe: 3.171 ± 0.067	5.514GlyGly: 5.514 ± 0.153	1.442GlyHis: 1.442 ± 0.048	6.313GlyIle: 6.313 ± 0.093	5.894GlyLys: 5.894 ± 0.083	6.137GlyLeu: 6.137 ± 0.094	2.69GlyMet: 2.69 ± 0.058	3.399GlyAsn: 3.399 ± 0.13	1.477GlyPro: 1.477 ± 0.044	2.116GlyGln: 2.116 ± 0.059	3.307GlyArg: 3.307 ± 0.073	4.53GlySer: 4.53 ± 0.093	4.94GlyThr: 4.94 ± 0.096	5.122GlyVal: 5.122 ± 0.086	0.861GlyTrp: 0.861 ± 0.043	3.223GlyTyr: 3.223 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	1.239HisAla: 1.239 ± 0.041	0.291HisCys: 0.291 ± 0.017	0.9HisAsp: 0.9 ± 0.038	1.194HisGlu: 1.194 ± 0.036	0.812HisPhe: 0.812 ± 0.031	1.382HisGly: 1.382 ± 0.044	0.442HisHis: 0.442 ± 0.044	1.254HisIle: 1.254 ± 0.041	0.881HisLys: 0.881 ± 0.03	1.543HisLeu: 1.543 ± 0.05	0.663HisMet: 0.663 ± 0.032	0.656HisAsn: 0.656 ± 0.028	0.938HisPro: 0.938 ± 0.036	0.459HisGln: 0.459 ± 0.024	0.826HisArg: 0.826 ± 0.032	0.94HisSer: 0.94 ± 0.032	0.999HisThr: 0.999 ± 0.04	1.271HisVal: 1.271 ± 0.043	0.164HisTrp: 0.164 ± 0.014	0.69HisTyr: 0.69 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.668IleAla: 5.668 ± 0.092	1.268IleCys: 1.268 ± 0.045	3.819IleAsp: 3.819 ± 0.074	4.305IleGlu: 4.305 ± 0.074	2.813IlePhe: 2.813 ± 0.062	5.177IleGly: 5.177 ± 0.089	1.323IleHis: 1.323 ± 0.04	4.266IleIle: 4.266 ± 0.083	3.548IleLys: 3.548 ± 0.067	6.694IleLeu: 6.694 ± 0.109	1.923IleMet: 1.923 ± 0.053	2.537IleAsn: 2.537 ± 0.057	3.368IlePro: 3.368 ± 0.06	2.003IleGln: 2.003 ± 0.052	3.886IleArg: 3.886 ± 0.08	4.385IleSer: 4.385 ± 0.072	3.948IleThr: 3.948 ± 0.072	4.662IleVal: 4.662 ± 0.078	0.613IleTrp: 0.613 ± 0.028	2.502IleTyr: 2.502 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.206LysAla: 5.206 ± 0.093	0.639LysCys: 0.639 ± 0.03	3.925LysAsp: 3.925 ± 0.077	6.518LysGlu: 6.518 ± 0.098	2.021LysPhe: 2.021 ± 0.041	3.97LysGly: 3.97 ± 0.076	1.005LysHis: 1.005 ± 0.034	4.763LysIle: 4.763 ± 0.071	6.281LysLys: 6.281 ± 0.098	5.236LysLeu: 5.236 ± 0.085	2.276LysMet: 2.276 ± 0.049	3.591LysAsn: 3.591 ± 0.071	2.095LysPro: 2.095 ± 0.056	2.159LysGln: 2.159 ± 0.058	3.41LysArg: 3.41 ± 0.067	3.239LysSer: 3.239 ± 0.071	3.955LysThr: 3.955 ± 0.062	4.038LysVal: 4.038 ± 0.074	0.606LysTrp: 0.606 ± 0.03	2.572LysTyr: 2.572 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	7.449LeuAla: 7.449 ± 0.104	1.426LeuCys: 1.426 ± 0.042	5.015LeuAsp: 5.015 ± 0.073	6.034LeuGlu: 6.034 ± 0.085	3.796LeuPhe: 3.796 ± 0.082	6.16LeuGly: 6.16 ± 0.094	1.487LeuHis: 1.487 ± 0.044	5.584LeuIle: 5.584 ± 0.093	6.192LeuLys: 6.192 ± 0.086	8.099LeuLeu: 8.099 ± 0.117	2.619LeuMet: 2.619 ± 0.061	3.646LeuAsn: 3.646 ± 0.068	3.588LeuPro: 3.588 ± 0.069	2.418LeuGln: 2.418 ± 0.056	3.691LeuArg: 3.691 ± 0.071	6.03LeuSer: 6.03 ± 0.096	5.118LeuThr: 5.118 ± 0.084	5.506LeuVal: 5.506 ± 0.094	0.759LeuTrp: 0.759 ± 0.032	3.23LeuTyr: 3.23 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.797MetAla: 2.797 ± 0.051	0.345MetCys: 0.345 ± 0.02	1.987MetAsp: 1.987 ± 0.048	2.745MetGlu: 2.745 ± 0.056	1.184MetPhe: 1.184 ± 0.037	2.31MetGly: 2.31 ± 0.062	0.435MetHis: 0.435 ± 0.023	2.297MetIle: 2.297 ± 0.06	2.731MetLys: 2.731 ± 0.062	2.57MetLeu: 2.57 ± 0.056	1.051MetMet: 1.051 ± 0.035	1.55MetAsn: 1.55 ± 0.046	1.172MetPro: 1.172 ± 0.039	0.986MetGln: 0.986 ± 0.032	1.402MetArg: 1.402 ± 0.037	1.843MetSer: 1.843 ± 0.041	1.891MetThr: 1.891 ± 0.046	2.028MetVal: 2.028 ± 0.049	0.235MetTrp: 0.235 ± 0.018	0.879MetTyr: 0.879 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.228AsnAla: 3.228 ± 0.07	0.555AsnCys: 0.555 ± 0.03	1.98AsnAsp: 1.98 ± 0.052	2.653AsnGlu: 2.653 ± 0.058	1.602AsnPhe: 1.602 ± 0.047	3.828AsnGly: 3.828 ± 0.121	0.717AsnHis: 0.717 ± 0.03	2.91AsnIle: 2.91 ± 0.062	2.222AsnLys: 2.222 ± 0.057	3.564AsnLeu: 3.564 ± 0.071	1.451AsnMet: 1.451 ± 0.045	1.56AsnAsn: 1.56 ± 0.059	2.026AsnPro: 2.026 ± 0.047	1.307AsnGln: 1.307 ± 0.048	2.102AsnArg: 2.102 ± 0.05	2.339AsnSer: 2.339 ± 0.049	2.22AsnThr: 2.22 ± 0.061	2.829AsnVal: 2.829 ± 0.058	0.384AsnTrp: 0.384 ± 0.026	1.603AsnTyr: 1.603 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.965ProAla: 2.965 ± 0.072	0.456ProCys: 0.456 ± 0.022	2.525ProAsp: 2.525 ± 0.055	3.727ProGlu: 3.727 ± 0.073	1.497ProPhe: 1.497 ± 0.046	2.794ProGly: 2.794 ± 0.071	0.578ProHis: 0.578 ± 0.026	2.089ProIle: 2.089 ± 0.044	1.955ProLys: 1.955 ± 0.049	2.804ProLeu: 2.804 ± 0.065	1.003ProMet: 1.003 ± 0.033	1.156ProAsn: 1.156 ± 0.039	0.784ProPro: 0.784 ± 0.036	0.988ProGln: 0.988 ± 0.036	1.05ProArg: 1.05 ± 0.036	1.795ProSer: 1.795 ± 0.043	1.667ProThr: 1.667 ± 0.047	3.228ProVal: 3.228 ± 0.059	0.341ProTrp: 0.341 ± 0.019	1.483ProTyr: 1.483 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.489GlnAla: 2.489 ± 0.055	0.319GlnCys: 0.319 ± 0.018	1.478GlnAsp: 1.478 ± 0.047	2.303GlnGlu: 2.303 ± 0.049	1.081GlnPhe: 1.081 ± 0.036	1.88GlnGly: 1.88 ± 0.051	0.429GlnHis: 0.429 ± 0.023	2.189GlnIle: 2.189 ± 0.051	2.387GlnLys: 2.387 ± 0.062	2.348GlnLeu: 2.348 ± 0.05	1.139GlnMet: 1.139 ± 0.038	1.478GlnAsn: 1.478 ± 0.048	0.881GlnPro: 0.881 ± 0.034	0.913GlnGln: 0.913 ± 0.036	1.293GlnArg: 1.293 ± 0.037	1.547GlnSer: 1.547 ± 0.04	1.739GlnThr: 1.739 ± 0.045	1.926GlnVal: 1.926 ± 0.044	0.311GlnTrp: 0.311 ± 0.021	1.327GlnTyr: 1.327 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	3.115ArgAla: 3.115 ± 0.065	0.665ArgCys: 0.665 ± 0.03	2.395ArgAsp: 2.395 ± 0.061	3.943ArgGlu: 3.943 ± 0.084	1.917ArgPhe: 1.917 ± 0.048	2.762ArgGly: 2.762 ± 0.06	0.81ArgHis: 0.81 ± 0.031	3.65ArgIle: 3.65 ± 0.064	3.513ArgLys: 3.513 ± 0.074	4.049ArgLeu: 4.049 ± 0.08	1.641ArgMet: 1.641 ± 0.044	1.975ArgAsn: 1.975 ± 0.055	1.472ArgPro: 1.472 ± 0.04	1.666ArgGln: 1.666 ± 0.05	2.567ArgArg: 2.567 ± 0.059	2.329ArgSer: 2.329 ± 0.057	2.473ArgThr: 2.473 ± 0.054	2.829ArgVal: 2.829 ± 0.062	0.362ArgTrp: 0.362 ± 0.018	1.861ArgTyr: 1.861 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.483SerAla: 4.483 ± 0.078	0.793SerCys: 0.793 ± 0.027	3.235SerAsp: 3.235 ± 0.067	3.829SerGlu: 3.829 ± 0.074	2.533SerPhe: 2.533 ± 0.049	5.599SerGly: 5.599 ± 0.091	0.993SerHis: 0.993 ± 0.039	3.753SerIle: 3.753 ± 0.075	2.935SerLys: 2.935 ± 0.065	4.911SerLeu: 4.911 ± 0.078	1.951SerMet: 1.951 ± 0.055	2.081SerAsn: 2.081 ± 0.061	1.745SerPro: 1.745 ± 0.049	1.719SerGln: 1.719 ± 0.052	2.695SerArg: 2.695 ± 0.072	3.503SerSer: 3.503 ± 0.077	2.922SerThr: 2.922 ± 0.062	4.269SerVal: 4.269 ± 0.067	0.594SerTrp: 0.594 ± 0.024	2.206SerTyr: 2.206 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	5.226ThrAla: 5.226 ± 0.102	0.71ThrCys: 0.71 ± 0.029	3.258ThrAsp: 3.258 ± 0.069	4.083ThrGlu: 4.083 ± 0.077	2.243ThrPhe: 2.243 ± 0.05	5.103ThrGly: 5.103 ± 0.09	0.85ThrHis: 0.85 ± 0.036	3.796ThrIle: 3.796 ± 0.075	3.158ThrLys: 3.158 ± 0.056	4.715ThrLeu: 4.715 ± 0.082	1.555ThrMet: 1.555 ± 0.048	1.948ThrAsn: 1.948 ± 0.052	2.32ThrPro: 2.32 ± 0.057	1.316ThrGln: 1.316 ± 0.043	2.038ThrArg: 2.038 ± 0.048	2.901ThrSer: 2.901 ± 0.068	3.067ThrThr: 3.067 ± 0.08	4.729ThrVal: 4.729 ± 0.086	0.545ThrTrp: 0.545 ± 0.028	1.987ThrTyr: 1.987 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.909ValAla: 4.909 ± 0.097	1.275ValCys: 1.275 ± 0.04	3.906ValAsp: 3.906 ± 0.077	4.644ValGlu: 4.644 ± 0.08	3.054ValPhe: 3.054 ± 0.064	4.517ValGly: 4.517 ± 0.084	1.135ValHis: 1.135 ± 0.037	5.262ValIle: 5.262 ± 0.086	4.523ValLys: 4.523 ± 0.088	6.669ValLeu: 6.669 ± 0.108	2.131ValMet: 2.131 ± 0.054	2.861ValAsn: 2.861 ± 0.06	2.856ValPro: 2.856 ± 0.053	1.892ValGln: 1.892 ± 0.054	3.151ValArg: 3.151 ± 0.075	4.466ValSer: 4.466 ± 0.073	4.352ValThr: 4.352 ± 0.08	4.867ValVal: 4.867 ± 0.091	0.571ValTrp: 0.571 ± 0.025	2.55ValTyr: 2.55 ± 0.063	0.0ValXaa: 0.0 ± 0.0
Trp	0.537TrpAla: 0.537 ± 0.025	0.142TrpCys: 0.142 ± 0.013	0.546TrpAsp: 0.546 ± 0.028	0.665TrpGlu: 0.665 ± 0.03	0.418TrpPhe: 0.418 ± 0.03	0.64TrpGly: 0.64 ± 0.03	0.166TrpHis: 0.166 ± 0.012	0.628TrpIle: 0.628 ± 0.031	0.785TrpLys: 0.785 ± 0.032	0.875TrpLeu: 0.875 ± 0.035	0.337TrpMet: 0.337 ± 0.018	0.503TrpAsn: 0.503 ± 0.025	0.2TrpPro: 0.2 ± 0.016	0.364TrpGln: 0.364 ± 0.022	0.398TrpArg: 0.398 ± 0.022	0.456TrpSer: 0.456 ± 0.021	0.428TrpThr: 0.428 ± 0.026	0.534TrpVal: 0.534 ± 0.026	0.118TrpTrp: 0.118 ± 0.013	0.477TrpTyr: 0.477 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.808TyrAla: 2.808 ± 0.053	0.586TyrCys: 0.586 ± 0.027	2.463TyrAsp: 2.463 ± 0.057	2.819TyrGlu: 2.819 ± 0.058	1.71TyrPhe: 1.71 ± 0.043	3.014TyrGly: 3.014 ± 0.063	0.819TyrHis: 0.819 ± 0.029	2.398TyrIle: 2.398 ± 0.057	2.03TyrLys: 2.03 ± 0.054	3.626TyrLeu: 3.626 ± 0.071	1.08TyrMet: 1.08 ± 0.039	1.603TyrAsn: 1.603 ± 0.047	1.402TyrPro: 1.402 ± 0.04	1.294TyrGln: 1.294 ± 0.041	2.096TyrArg: 2.096 ± 0.052	2.123TyrSer: 2.123 ± 0.048	2.129TyrThr: 2.129 ± 0.055	2.578TyrVal: 2.578 ± 0.059	0.335TyrTrp: 0.335 ± 0.021	1.735TyrTyr: 1.735 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2993 proteins (854591 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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