Enterococcus sulfureus ATCC 49903

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus sulfureus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S0P0H6|S0P0H6_9ENTE Glucose-1-phosphate adenylyltransferase GlgD subunit OS=Enterococcus sulfureus ATCC 49903 OX=1140003 GN=I573_00119 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 4.06HH4 pKa = 5.93THH6 pKa = 6.26DD7 pKa = 4.18HH8 pKa = 5.82NHH10 pKa = 6.13DD11 pKa = 3.93HH12 pKa = 6.86DD13 pKa = 5.05HH14 pKa = 7.0EE15 pKa = 4.46GHH17 pKa = 5.2EE18 pKa = 5.19HH19 pKa = 5.75ITLVDD24 pKa = 3.5NEE26 pKa = 4.5GNEE29 pKa = 4.1TLYY32 pKa = 10.99EE33 pKa = 4.03ILLTIDD39 pKa = 3.3GQEE42 pKa = 3.92EE43 pKa = 4.06FGRR46 pKa = 11.84NYY48 pKa = 10.54VLLYY52 pKa = 9.37PAGVPEE58 pKa = 4.94DD59 pKa = 3.99EE60 pKa = 5.07DD61 pKa = 4.53VEE63 pKa = 4.41LLAYY67 pKa = 10.17AYY69 pKa = 10.29VEE71 pKa = 4.7KK72 pKa = 10.43EE73 pKa = 4.01DD74 pKa = 4.07GMEE77 pKa = 4.51GDD79 pKa = 4.48LEE81 pKa = 4.23QIEE84 pKa = 4.77TEE86 pKa = 4.57KK87 pKa = 10.41EE88 pKa = 3.02WDD90 pKa = 3.5MIEE93 pKa = 4.35EE94 pKa = 4.2VFNTFMAEE102 pKa = 4.02EE103 pKa = 3.96EE104 pKa = 4.43AEE106 pKa = 4.09

Molecular weight:
12.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S0P1D5|S0P1D5_9ENTE ABC transmembrane type-1 domain-containing protein OS=Enterococcus sulfureus ATCC 49903 OX=1140003 GN=I573_00689 PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.15QKK5 pKa = 7.56THH7 pKa = 6.75RR8 pKa = 11.84GLAKK12 pKa = 9.82RR13 pKa = 11.84VKK15 pKa = 8.84RR16 pKa = 11.84TGNGGLKK23 pKa = 10.07RR24 pKa = 11.84FRR26 pKa = 11.84AFTSHH31 pKa = 6.94RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.68ASMVSKK52 pKa = 10.82GDD54 pKa = 3.43YY55 pKa = 10.18KK56 pKa = 10.9RR57 pKa = 11.84IRR59 pKa = 11.84QQLARR64 pKa = 11.84MKK66 pKa = 10.67

Molecular weight:
7.86 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2176

0

2176

687799

29

3284

316.1

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.519 ± 0.053

0.606 ± 0.015

5.164 ± 0.043

7.041 ± 0.06

4.634 ± 0.051

6.332 ± 0.05

2.056 ± 0.027

7.447 ± 0.053

6.283 ± 0.048

9.976 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.027

4.039 ± 0.042

3.553 ± 0.036

4.757 ± 0.048

3.786 ± 0.046

5.954 ± 0.162

6.678 ± 0.085

6.963 ± 0.043

0.929 ± 0.02

3.71 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski