Streptococcus phage Javan617

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AQ76|A0A4D6AQ76_9CAUD Uncharacterized protein OS=Streptococcus phage Javan617 OX=2548283 GN=Javan617_0047 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 10.54KK3 pKa = 10.47LYY5 pKa = 9.38YY6 pKa = 9.43TYY8 pKa = 11.09RR9 pKa = 11.84NYY11 pKa = 10.6TSEE14 pKa = 4.37DD15 pKa = 3.74LIEE18 pKa = 5.28AIQEE22 pKa = 3.99LTDD25 pKa = 4.62DD26 pKa = 4.0KK27 pKa = 11.6FEE29 pKa = 5.95LIINTIAGSNITIKK43 pKa = 10.61QEE45 pKa = 3.65ILNLGEE51 pKa = 3.95

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AQA9|A0A4D6AQA9_9CAUD Terminase large subunit OS=Streptococcus phage Javan617 OX=2548283 GN=Javan617_0023 PE=4 SV=1
MM1 pKa = 7.59DD2 pKa = 3.75VKK4 pKa = 11.3VKK6 pKa = 10.73VDD8 pKa = 3.74LKK10 pKa = 10.88GLEE13 pKa = 4.45KK14 pKa = 10.46KK15 pKa = 10.61VSPQSFARR23 pKa = 11.84GQLAMTNQMLLDD35 pKa = 3.66MNKK38 pKa = 9.42YY39 pKa = 9.34VPARR43 pKa = 11.84SEE45 pKa = 3.95ALRR48 pKa = 11.84SSGHH52 pKa = 5.96ANKK55 pKa = 9.01DD56 pKa = 3.56TIIWSSPYY64 pKa = 10.89ARR66 pKa = 11.84IRR68 pKa = 11.84FYY70 pKa = 11.23NRR72 pKa = 11.84KK73 pKa = 9.41LKK75 pKa = 10.95LFFSDD80 pKa = 2.86KK81 pKa = 9.42QRR83 pKa = 11.84RR84 pKa = 11.84FFFANKK90 pKa = 9.98DD91 pKa = 3.13KK92 pKa = 11.27LLAQKK97 pKa = 9.16PRR99 pKa = 11.84PGTGPRR105 pKa = 11.84WDD107 pKa = 4.19LKK109 pKa = 11.3AKK111 pKa = 10.25AKK113 pKa = 10.5HH114 pKa = 5.67GNDD117 pKa = 3.07WGKK120 pKa = 10.3VALKK124 pKa = 10.87GMGISKK130 pKa = 10.51

Molecular weight: 14.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10273

51

1176

197.6

22.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.97 ± 1.125

0.428 ± 0.092

6.434 ± 0.485

6.96 ± 0.676

4.439 ± 0.259

6.269 ± 0.649

1.382 ± 0.182

7.885 ± 0.274

8.342 ± 0.616

8.274 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.152

5.617 ± 0.279

2.833 ± 0.233

3.631 ± 0.237

4.011 ± 0.278

7.077 ± 0.437

6.113 ± 0.446

5.753 ± 0.304

0.964 ± 0.119

4.049 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski